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Effects of growth factors on growth and functions of endocrine cells

Research Project

Project/Area Number 61480254
Research Category

Grant-in-Aid for General Scientific Research (B)

Allocation TypeSingle-year Grants
Research Field 内分泌・代謝学
Research InstitutionTokyo Women's Medical College

Principal Investigator

TOSHIO Tsushima  Tokyo Women's Medical College, 内科2, 教授 (90101089)

Co-Investigator(Kenkyū-buntansha) MOTOYASU Saji  Tokyo Women's Medical College, 内科2, 助手 (80162395)
YOSHITO Ohba  Tokyo Women's Medical College, 内科2, 助手 (90160613)
EIJI Ohmura  Tokyo Women's Medical College, 内科2, 助手 (90119917)
Project Period (FY) 1986 – 1988
Project Status Completed (Fiscal Year 1988)
Budget Amount *help
¥5,700,000 (Direct Cost: ¥5,700,000)
Fiscal Year 1988: ¥1,500,000 (Direct Cost: ¥1,500,000)
Fiscal Year 1987: ¥1,500,000 (Direct Cost: ¥1,500,000)
Fiscal Year 1986: ¥2,700,000 (Direct Cost: ¥2,700,000)
Keywordsthyroid / parathyoid / growth factors / IGF-I / EGF / TGF- TGF- / autocrine factor / オートクリン因子 / 甲状腺癌 / 甲状腺細胞 / 細胞増殖 / ヨード取りこみ / TGFβ / TGFα / TSH / ヨード代謝 / 受容体 / MSA
Research Abstract

The effects of peptide growth factors on proliferation and differentialed functions of endocrine cells were studied. Thyroid cells of human, pig and rat were found have receptors for insulin-lile growth factor (IGF)-i and IGF-II. igfs stimulated DNA synthesis and growth of cultured porcine thyroid cells and the effect was potentiated by epidermal growth factor (EGF). Furhtermore, IGF-I stimulated TSH-induced iodide uptake and organification without change in TSH-stimulated cAMP production. preliminary studies have also shown that IGF-I stimulate thyroglobulin production. Tissue content of immunoreactive IGF-I and IGF-I binding proteins were significantly higher in human papillary cancer than in surrounding normal tissues. Medium conditioned by an established human thyroid cancer cells contained IGF-like activity and mRNA for IGF-I was identified by northern blotting. Furthermore, growth of this cell line was inhibited by antigody to IGF-I receptors. This obervations suggest that IGF-I act as an autocrine growth facator in some of human thyroid cancer cells. We also found that the size of goiter is positively correlated with plasma concentration of IGF-I in acromegaly. Thus, IGF-I may be involved in growth and functions of thyroid cells. TGF- , like EGF, stimulated growth but inhibited differentiated functions of procine thyroid cells. On the other hand, TAF- inhibited growth stimulated be either IGF-I or TGF- and TSH-insuced iodine metabolism. The inhibitory effect was not mediated by inhibition of cAMP production. Platelet-derived growth factor (PDGF) or fibroblast growth factotor (FGF) was without effect on growth and differentiated functions of thyroid cells. We also identified IGF-I and IGF-II receptors in human parathyroid cells. IGF-I, IGF-II, EGF and IGF stimulated DNA synthesis. Thus, we found that many growth factors affect growth and functions of endocrine cells.

Report

(4 results)
  • 1988 Annual Research Report   Final Research Report Summary
  • 1987 Annual Research Report
  • 1986 Annual Research Report
  • Research Products

    (29 results)

All Other

All Publications (29 results)

  • [Publications] Tsushima T;Ohba Y;Emoto N;Shizume K;Imai: Biochem Biophys Res Commun. 133. 951-957 (1985)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Saji M;Tsushima T; Isozaki O;Murakami H;Ohba Y;Sato K;Arai M;Shizume K: Endocrinology. 121. 749-756 (1987)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Saji M;Isozaki O;Tsushima T;Arai M;Miyakawa M;Ohba Y;Tsuchiya Y;et al.: Acta Endocrinol. 119. 145-151 (1988)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Ozawa M;Sato K;Han DC;Kawakami M;Tsushima T;Shizume K: Endocrinology. 123. 1461-1467 (1988)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Tsushima T;Arai M;Saji M;Ohba Y;Murakami H;Ohmura E;Shizume K: Endocrinology. 123. 1178-1194 (1988)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Tsushima T: Gunma Symposia on Endocrinology. 25. 149-166 (1988)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Tsushima,T; Ohba,Y; Emoto,N; Shizume,K; Imai Y: "Identification and initial characterization of transforming growth factor-like mitogens in human anterior pituitary" Biochem Biophy Res Commun. 133. 951-957 (1985)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Saji,M; Tsushima,T; Isozaki,O; Murakami,H; Ohba,Y; Sato,K; Arai,M; Shizume,K.: "Interaction of insulin-like growth factor I with porcine thyroid cells cultured in monolayer" Endocrinology. 121. 749-756 (1987)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Saji,M; Isozaki,O; Tsushima,T; Arai,M; Miyakawa,M; Ohba,Y; Tsuchiya,Y; Shizume,K.: "The inhibitory effect of iodine on growth of rat thyroid (FRTL-5) cells" Acta Endocrinol. 119. 145-151 (1988)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Ozawa,M; Sato,K; Han,DC; Kawakami,M; Tsushima,T; Shizume,K.: "Effects of tumor necrosis factor- /cachectin on thyroid hormone metabolism in mice." Endocrinology. 123. 1461-1467 (1988)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Tsushima,T; Arai,M; Saji,M; Ohba,Y; Murakami,H; Ohmura,E; Shizume,K.: "Effects of transforming growth factor- on deoxyribonucleic acid synthesis and iodine metabolism in porcine thyorid cells in culture." Endocrinology. 123. 1178-1194 (1988)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Ozawa.M;Sato.K;Han.DC;Kawakami.M;Tsushima.T;Shizume.K,: Endocrinology. 123. 1461-1467 (1988)

    • Related Report
      1988 Annual Research Report
  • [Publications] Saji.M;Isozaki.O;Tsushima.T;Arai.M;Miyakawa.M;Ohba.Y;Tsuchiya.Y;Sano.T,: Acta Endocrinol(Copenh). 119. 145-151 (1988)

    • Related Report
      1988 Annual Research Report
  • [Publications] Tsushima.T;Arai.M;Saji.M;Ohba.Y;Murakami.H;Ohmura.E;Shizume.K,: Endocrinology. 123. 1178-1194 (1988)

    • Related Report
      1988 Annual Research Report
  • [Publications] Miyakawa.M;Saji.M;Tsushima.T;Wakai.K;Shizume.K,: J Clin Endocrinol Metab. 67. 973-977 (1988)

    • Related Report
      1988 Annual Research Report
  • [Publications] Tsushima.T.: Gunma Symposia on Endocrinology. 25. 149-166 (1988)

    • Related Report
      1988 Annual Research Report
  • [Publications] Ohmura.E;Okada.M;Ohba.Y;Onoda.N;Sano T;Tsushima.T;Shizume.K.: J Endocrinol. 118. 423 (1988)

    • Related Report
      1988 Annual Research Report
  • [Publications] Emoto.N;Tsushima.T;Shizume.K;et al: Acta Endocrinologica. 114. 283-291 (1987)

    • Related Report
      1987 Annual Research Report
  • [Publications] Sato.K;Han.D.C;Fujii.Y;Thushima.T;and.Shizume.K: Endocrinology. 120. 1873-1881 (1987)

    • Related Report
      1987 Annual Research Report
  • [Publications] Ohmura.E;Tshushima.T;Emoto.N;et al: Life Science. 41. 691-696 (1987)

    • Related Report
      1987 Annual Research Report
  • [Publications] Saji.M;Tshushima.T.et al: Endocrinology. 121. 749-756 (1987)

    • Related Report
      1987 Annual Research Report
  • [Publications] Ohmura.E;Emoto.N;Tshushima.T.et al: Hepato-Gastroenterology. 34. 1044-1048 (1987)

    • Related Report
      1987 Annual Research Report
  • [Publications] Tsushima.T;et al: Endocrinology. (1988)

    • Related Report
      1987 Annual Research Report
  • [Publications] Isozaki O;Tsushima T;Shizume K et.al.: J Clinical Endocrinology and Metabolism. 61. 1105-1111 (1985)

    • Related Report
      1986 Annual Research Report
  • [Publications] Hizuka N;Takano K;Tanaka I;Honda N;Tsushima T;Shizume K: J Clinical Endocrnology and Metabolism. 61. 1066-1085 (1985)

    • Related Report
      1986 Annual Research Report
  • [Publications] Han DC;Sato K;Fujii Y;Tsushima T;Shizume K: Endocrinology. 119. 1076-1082 (1986)

    • Related Report
      1986 Annual Research Report
  • [Publications] Masuda A;Tsushima T et al: J Clinical Endocrinology Investigation. (1986)

    • Related Report
      1986 Annual Research Report
  • [Publications] Emoto N;Tsushima T et al.: Acta Endocrinol. 114. 283-291 (1987)

    • Related Report
      1986 Annual Research Report
  • [Publications] Saji M;Tsushima T et al.: Endocrinology. (1987)

    • Related Report
      1986 Annual Research Report

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Published: 1987-03-31   Modified: 2016-04-21  

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