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Regulation of gene expression in erythropoiesis

Research Project

Project/Area Number 61480428
Research Category

Grant-in-Aid for General Scientific Research (B)

Allocation TypeSingle-year Grants
Research Field Biological pharmacy
Research InstitutionTOHOKU UNIVERSITY (1987)
The University of Tokyo (1986)

Principal Investigator

OBINATA Masuo  The Research Institute for Tuberculosis & Cancer, Tohoku Univ., 抗酸菌病研究所, 教授 (10099971)

Project Period (FY) 1986 – 1987
Project Status Completed (Fiscal Year 1987)
Budget Amount *help
¥6,800,000 (Direct Cost: ¥6,800,000)
Fiscal Year 1987: ¥2,000,000 (Direct Cost: ¥2,000,000)
Fiscal Year 1986: ¥4,800,000 (Direct Cost: ¥4,800,000)
Keywordserythropoiesis / erythropoietin / CFUE / commitment / c-myc gene / 細胞内シグナル伝達 / CFUーE / cーmyc遺伝子 / CFU-E / src遺伝子
Research Abstract

The induced differentiation of murine erythroleukemia(MEL) cells in vitro is a good system to analyze the molecular events underlining the commitment process of cell differentiation. We found that TSA8 cells, one of MEL cells were committed to respond to erythropoietin(epo) and to form the differentiated colonies of erythroid progenitor cells, when the cells were treated with DMSO. In the induced process, the surface receptors for epo did not change in their number and in their affinities, thus, the intracellular factors induced in the cells are required for the acquisition of responsiveness to epo. The involvement of two independent factors are shown to be required for the commitment; one is the cycloheximide-sensitive factor which appears just after the addition of DMSO, the other is the Na^+/H^+ transporter which is sensitive to anyloride derivatives and appears in the later process of commitment. Thus, the commitment may be controlled by the sequential appearance of two factors, The signal transduction induced by epo was analyzed and the results indicated that a cAMP pathway is mainly involved in the epo action.
To examine the involvement of cellular onco genes in the commitment process, the methallothionein promoter-linked c-myc gene was introduced into MEL cells and the effect of the regulated, but over-produced c-myc products was analyzed. The results indicated that the level of c-myc determines the probability of commitment of differentiation of Mel cells, Furthermore, c-myc may function to link the commitment process and the timing of expression of tissue-specific genes.

Report

(3 results)
  • 1987 Annual Research Report   Final Research Report Summary
  • 1986 Annual Research Report
  • Research Products

    (22 results)

All Other

All Publications (22 results)

  • [Publications] Y. Mishina;T. Kato;A. Urabe;F. Takaku;S. Natori & M. Obinata: Develop. Growth & Different.28. 1-6 (1986)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] Y. Kobayashi;D. Miyamoto;M. Asada;M. Obinate & T. Osawa: J. Biochem.100. 727-733 (1986)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] S. Takada & M. Obinata: Cell Differentiation. 21. 111-118 (1987)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] M. Noguchi;H. Fukumoto;Y. Mishina & M. Obinata: Development. 101. 169-174 (1987)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] Y. Mishina;Y. Matsui & M. Obinata: Cell Differentiation.

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] Y,Mishina, T,Kato, AUrabe, F,Takaku, S,Natori & M,Obinata: "Unique pattern of gene expression in the erythroid precursor cells" Develp. Growth & Different. 28. 1-6 (1986)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] Y,Kobayashi, D,Miyamoto, M,Asada, M,Obinata & T,Osawa: "Cloning and expression of human lymphotoxin mRNA derived from a human T cell hybridoma" J. Biochem.100. 727-733 (1986)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] K,Kawamura, H,Satow, L.Do Id, S,Sakai, T,Takada & M,Obinata: "Modulation of transferred mouse 26k casein gene into mouse L cells with glucocorticoid hormone" J. Biochem.101. 103-110 (1987)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] S,Takada & M,Obinata: "Characterization of trans-acting factor(s) regulating <beta>-globin gene expression in in vivo competition" Cell differentiation. 21. 1118-118 (1987)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] T,Noguchi, H,Fukumoto, Y,Mishina & M,Obinata: "Factors controllin ginduction of commitment of murine erythroleukemia (TSA8) cells of CFR-E(colony forming unit-erythroid)" Development. 101. 169-174 (1987)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] Y.Mishina;T.Kato;A.Urabe;F.Takaku;S.Natori;M.Obinata: Develop.Growth & Different.28. 1-6 (1986)

    • Related Report
      1987 Annual Research Report
  • [Publications] Y.Kobayashi;D.Miyamoto;M.Asada;M.Obinata;T.Osawa: J.Biochem.100. 727-733 (1986)

    • Related Report
      1987 Annual Research Report
  • [Publications] K.Kawamura;H.Satow;L.Do IK;S.Sakai;T.Takada;M.Obinata: J.Biochem.101. 103-110 (1987)

    • Related Report
      1987 Annual Research Report
  • [Publications] S.Takada;M.Obinata: Cell Differentiation. 21. 111-118 (1987)

    • Related Report
      1987 Annual Research Report
  • [Publications] T.Noguchi;H.Fukumoto;Y.Mishina;M.Obinata: Development. 101. 169-174 (1987)

    • Related Report
      1987 Annual Research Report
  • [Publications] Y.Mishina;Y.Matsui;M.Obinata: Cell Differentiation.

    • Related Report
      1987 Annual Research Report
  • [Publications] Y.Mishina;T.Kato;A.Urabe;F.Takaku;S.Natori;M.Obinata: Develop.Growth & Different.28. 1-6 (1986)

    • Related Report
      1986 Annual Research Report
  • [Publications] H.Satow;S.Sakai;M.Obinata: J.Biochem.99. 1639-1643 (1986)

    • Related Report
      1986 Annual Research Report
  • [Publications] Y.Kobayashi;D.Miyamoto;M.Asada;M.Obinata;T.Osawa: J.Biochem.100. 727-733 (1986)

    • Related Report
      1986 Annual Research Report
  • [Publications] K.Kawamura;H.Satow;L.Do Ik;S.Sakai;T.Takada;M.Obinata: J.Biochem.101. 103-110 (1987)

    • Related Report
      1986 Annual Research Report
  • [Publications] I.Ishida;M.Obinata;T.Deguchi: J.Biol.Chem.

    • Related Report
      1986 Annual Research Report
  • [Publications] S.Takada,M.Obinata: Cell Differentiation.

    • Related Report
      1986 Annual Research Report

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Published: 1987-03-31   Modified: 2016-04-21  

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