| Project/Area Number |
61570601
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
General surgery
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| Research Institution | TOHOKU UNIVERSITY |
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Principal Investigator |
HAYASHI Yutaka Assistant Professor, Division of Pediatric Surgery, Tohoku Univ. Sch. Med., 医学部付属病院 小児外科学, 講師 (40125638)
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| Co-Investigator(Kenkyū-buntansha) |
KIMURA Michio Assistant Professor, Second Department of Surgery, TohokuUniv. Sch. Med., 医学部付属病院 第二外科学, 助手 (00142951)
NISHIHIRA Tetsuro Assistant Professor, Second Department of Surgery, Tohoku Univ. Sch. Med., 医学部第二外科学, 講師 (50101142)
OHI Ryoji Professor, Division of Pediatric Surgery, Tohoku Univ. Sch. Med., 医学部付属病院 小児外科学, 教授 (50004734)
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| Project Period (FY) |
1986 – 1987
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| Project Status |
Completed (Fiscal Year 1987)
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| Budget Amount *help |
¥2,100,000 (Direct Cost: ¥2,100,000)
Fiscal Year 1987: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 1986: ¥1,400,000 (Direct Cost: ¥1,400,000)
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| Keywords | Malignant tumor in childhood / Neuroblastoma / Flowcytometry / Malignant potentiality / Screening test of anticancer agents / モノクローナル抗体 / 抗癌剤スクリーニング / モノクロナル抗体 |
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| Research Abstract |
We projected to study malignant petentiality and to screen anticancer agents for malignant tumors in childhood using flowcytometric analysis. Three kinds of human neuroblastoma cell lines were used for the screening test of anticnacer agents (MMC,ADR,CDDP) by flowcytometric DNA analysis and its results were compared with the screening test by growth inhibition test. Flowcytometric procedure is excellent by its rapidity and quantitative analysis of cell damages. Cultured cells are needed to turn around the cell cycle at least one time for flowcytometric analysis. Accordingly, growth promotion of tumor cells in primary culture was a problem to be solved for the clinical application of this analysis. We found that the medium of cultured human monocytes from bone marrow included a substance which promoted growth of cultured neuroblastoma cells. The activity of this substance is suggested to correlate to granulocyte-macrophage conlony-stimulating factor. We think that this substance is extremely useful for the screening test of primary cultured cells.
We suceeded in producing of monoclonal antibodies against human neuroblastoma cell lines (TN-2,NB-1,GOTO). MWT-31 and T-93-13 are products of a cell fusion between X-63-Ag8-653 mouse myeloma cell line and spleen cells froma BALB/c mouse immunized with TN-2. MWT-31 binds to neuroblastoma cells and peripheral nerves. T-93-13 binds to neuroblastoma cells. N-14-4 is a product of those cells immunized with NB-1 and this antibody binds to neuroblastoma cells and peripheral nerves. G-16-2-D is a product of them immunized with GOTO and this binds to neuroblastoma cells. Antigens in neuroblastoma cell lines against MWT-31, N-14-4 and G-16-2-D increase by the addition of DB-cAMP or retinoic acid. These monoclonal antibodies seem to be useful for thediagnosis and treatment of neuroblastoma.
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