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Studies on the molecular breeding model by expression vectors

Research Project

Project/Area Number 62440008
Research Category

Grant-in-Aid for General Scientific Research (A)

Allocation TypeSingle-year Grants
Research Field Breeding science
Research InstitutionNagoya University

Principal Investigator

HIRAI Atsushi  Nagoya U., F.Agric. Assoc. Professor, 農学部, 助教授 (60023470)

Co-Investigator(Kenkyū-buntansha) YAMAGATA Hideo  Nagoya U., F.Agric. Assoc. Professor, 農学部, 助教授 (20023468)
HATTORI Kazumi  Nagoya U., F.Agric. Assis. Professor, 農学部, 助手 (40023494)
FUTSUHARA Yuzo  Nagoya U., F.Agric. Professor, 農学部, 教授 (70023405)
Project Period (FY) 1987 – 1989
Project Status Completed (Fiscal Year 1989)
Budget Amount *help
¥20,500,000 (Direct Cost: ¥20,500,000)
Fiscal Year 1989: ¥3,100,000 (Direct Cost: ¥3,100,000)
Fiscal Year 1988: ¥7,800,000 (Direct Cost: ¥7,800,000)
Fiscal Year 1987: ¥9,600,000 (Direct Cost: ¥9,600,000)
KeywordsRice / Chloroplast DNA / Mitochondrial DNA / Physical map / Molecular breeding / Cytoplasmic genome / Molecular cloning / 葉緑体ゲノム / psbA / クローニング / プロモーター / ターミネーター
Research Abstract

Chloroplast and mitochondrial genomes code for important genes for agriculture. However, since they are inherited maternally in most cases, it is difficult to improve these genomes by ordinally breeding methods. New breedings based on the molecular biology are expected to help programs to improve these genomes. As a model of the molecular breeding, we have conducted the analysis of molecular structures of chloroplast and mitochondrial genomes in rice.
1.Structure of chloroplast DNA from Oryza sativa A physical map and an overlapping clone bank of the ctDNA were constructed. Nucleotide sequences of genes which are important for photosynthesis, rbcL, atpB/E and psbA, were determined. The expression of these genes were also studied by S_1 mapping, primer extension and Northern analysis.
2.Complete nucleotide sequence of the ctDNA Using our clone bank, the complete nucleotide sequence of the ctDNA was determined. It is a circular and 134,525 bases long. All genes existed in tobacco ctDNA were also found in rice.
3.Cloning of ctDNA from wild species in the genus Oryza Chloroplast DNAs were isolated from Oryza australiensis O. punctata and O.officinalis, and fragments were cloned into lambda DNA. A physical map and an overlapping clone bank of each DNA were constructed.
4.Structure of mitochondrial DNA from oryza sativa Mitochondrial DNA was isolated from green leaves of Oryza sativa, and fragments were cloned into lambda DNA. A genetic and physical map of the DNA was constructed by "genome walking". The size of the master circle of mtDNA from rice is 528 kb. The DNA contain many fragments homologous to ctDNA fragments.

Report

(4 results)
  • 1989 Annual Research Report   Final Research Report Summary
  • 1988 Annual Research Report
  • 1987 Annual Research Report
  • Research Products

    (36 results)

All Other

All Publications (36 results)

  • [Publications] Nishizawa,Y.: "Nucleotide sequence and expression of the gene for the large subunit of rice ribulose 1,5-bisphosphate carboxylase." Jpn.J.Genet.62. 389-395 (1987)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Kanno,A.: "The nucleotide sequence and expression of the gene for the 32 kd quinone-binding protein from rice." Plant Science. 59. 95-99 (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Hiratsuka,J.: "The complete sequence of the rice(Oryza sativa)chloroplast genom." Mol.Gen.Genet.217. 185-194 (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Abe,T.: "Selection of higher regenerative callus and change in isozyme pattern in rice(Oryza sativa L.)" Theor.Appl.Genet.78. 648-652 (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Nishizawa.Y.: "The nucleotide sequence and expression of genes for the beta and epsilon subunits of ATp synthase from rice(Oryza sativa L.)" Jpn.J.Genet.64. 223-229 (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Shimada,H.: "A physical map and clone bank of the rice chloroplast genome." Plant Mol.Biol.Report.7. 284-291 (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Hirai,A.: "BIOTECHNOLOGY IN AGRICULTURE AND FORESTRY,Vol.14 Molecular Structure of Chloroplast DNA from Rice" Springer-Verlag, (inpress)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Nishizawa, Y.: "Nucleotide sequence and expression of the gene for the large subunit of rice ribulose 1, 5-bisphos-phate carboxylase." Jpn. J. Genet.62. 389-395 ((1987))

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Hirai, A.: "Analysis of chloroplast genoms in parasexual hybrid calli." Ciba Found. Symp. 137:113-118 (1988).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Yamagata, H.: "Use of Bacillus brevis for efficient synthesis and secretion of human epidermal growth factor." Proc. Natl. Acad. Sci. USA 86:3589-3593(1989).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Kanno, A.: "The nucleotide sequence and expression of the gene for the 32 kd quinone-binding protein from rice." Plant Science 59:95-99 (1989).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Balito, L. P.: "Effects of gamma-ray irradiation on the growth of calli in Nicotiana species." Japan. J. Breed. 39(1):29-37 (1989).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Hattori, K.: "Genetic studies on chloroplast proteins in rice. I. Simple analytical procedure for the analysis of chlorophyll protein complex." Japan. J. Breed.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Abe, T.: "Selection of higher regenerative callus and change in isozyme pattern in rice(Oryza sativa L.)" Theor. Appl. Genet. 78:648-652 (1989).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Hiratsuka, J.: "The complete sequence of the rice (Oryza sativa) chloroplast genome; Intermolecular recombination between distinct tRNA genes accounts for a major plastid DNA inversion during the evolution of the cereals." Mol. Gen. Genet. 217:185-194(1989).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Nishizawa, Y.: "The nucleotide sequences and expression of genes for the beta and epsilon subunits of ATP synthase from rice (Oryza sativa L.)." Jpn. J. Genet. 64:223-229 (1989).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Shimada, H.: "A physical map and clone bank of the rice (Oryza sativa) chloroplast genome." Plant Mol. Biol. Rep.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Hiratsuka,J.: "The complete sequence of the rice(Oryza sativa)chloroplast genome." Mol.Gen.Genet.217. 185-194 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] Nishizawa,Y.: "The nucleotide sequences and expression of genes for the beta and epsilon subunits of ATp synthase from rice(Oryza sativa L.)." Jpn.J.Genet.64. 223-229 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] Shimada,H.: "A physical map and clone bank of the rice chloroplast genome." Plant Mol.Biol.Report.7. 284-291 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] Abe,T.: "Selection of higher regenerative callus and change in isozyme pattern in rice(Oryza sativa L.)" Theor.Appl.Genet.78. 648-652 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] Yamagata,H.: "Use of Bacillus brevis for efficient synthesis and secretion of human epidermal growth factor." Proc.Natl.Acad.Sci.USA. 86. 3589-3593 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] Hattori,K.: "Genetic studies on chloroplast proteins in rice.I.Simple analytical procedure for the analysis of chlorophyll proetin complex." Japan.J.Breed.

    • Related Report
      1989 Annual Research Report
  • [Publications] Hirai,A.: "BIOTECHNOLOGY IN AGRICULTURE AND FORESTRY,Vol.14 Molecular Structure of Chloroplast DNA from Rice" SpringerーVerlag,

    • Related Report
      1989 Annual Research Report
  • [Publications] A.Kanno: Plant Science. 59. 95-99 (1989)

    • Related Report
      1988 Annual Research Report
  • [Publications] J.Hiratsuka: Mol.Gen.Genet.

    • Related Report
      1988 Annual Research Report
  • [Publications] Y.Nishizawa: Jpn.J.Genet.submitted.

    • Related Report
      1988 Annual Research Report
  • [Publications] L.P.Balito: Japan.J.Breed.39. 29-37 (1989)

    • Related Report
      1988 Annual Research Report
  • [Publications] ADACHI,Takahiro: Journal of Bacteriology.171. (1989)

    • Related Report
      1988 Annual Research Report
  • [Publications] YAMAGATA,Hideo: Proceedings of the National Academy of Sciences of the United States of America.

    • Related Report
      1988 Annual Research Report
  • [Publications] Nishizawa,Y.;Hirai,A.: Jpn. J. Genet.62. 389-395 (1987)

    • Related Report
      1987 Annual Research Report
  • [Publications] Akada,S.; Xu,Y.;Machii,H.;Hirai,A.; Kung,S.: Plant Physiol.83. 28 (1987)

    • Related Report
      1987 Annual Research Report
  • [Publications] Futsuhara,Y.;Hattori,H.; Kitano,H.: Rice Genet. Newsletter. 4. (1987)

    • Related Report
      1987 Annual Research Report
  • [Publications] Jia,Y.;Abe,T.;Futsuhara: Rice Genet. Newsletter. 4. (1987)

    • Related Report
      1987 Annual Research Report
  • [Publications] Yamagata,H.;Adachi,T.;Tsuboi,A.;Takao;M.Sasaki;Tsukagoshi,N.;Udaka,S.: J. Bacteriol.169. 1239-1245 (1987)

    • Related Report
      1987 Annual Research Report
  • [Publications] Kawazu,T.;Nakanishi,Y.;Uozumi,N.;Sasaki,T.;Yamagata,H.;Udaka,S.: J. Bacteriol.169. 1564-1570 (1987)

    • Related Report
      1987 Annual Research Report

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Published: 1987-04-01   Modified: 2016-04-21  

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