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Coupled Expression of The Genes Encoding The Constituents of The Glycine Cleavage System in Chicken

Research Project

Project/Area Number 62570101
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field General medical chemistry
Research InstitutionToyama Medical and Pharmaceutical University

Principal Investigator

HIRAGA Koichi  Department of Biochemistry, School of Medicine, Professor., 医学部, 教授 (40004733)

Co-Investigator(Kenkyū-buntansha) YAMAMOTO Masayuki  Department of Biochemistry, School of Medicine, Associate Professor., 医学部, 助教授 (50166823)
Project Period (FY) 1987 – 1988
Project Status Completed (Fiscal Year 1988)
Budget Amount *help
¥2,100,000 (Direct Cost: ¥2,100,000)
Fiscal Year 1988: ¥900,000 (Direct Cost: ¥900,000)
Fiscal Year 1987: ¥1,200,000 (Direct Cost: ¥1,200,000)
Keywordscoupled expression of the genes / glycine decarboxylase cDNA / H-protein cDNA / glycine cleavage system / 遺伝子の臓器特異的発現調節 / H-蛋白cDNA / 鶏ゲノムDNAライブラリー / グリシン脱炭酸酵素遺伝子 / H-蛋白遺伝子 / 高グリシン血症
Research Abstract

several clones of cDNA encoding qlycine decarboxylase, a constituent of the glycine cleavage system, were isolated from chicken liver cDNA expression libraries with a specific antibody. The overlapping cDNA clones evenly coded in an identical and open reading frame for the partial primary structures of the enzyme, and sequence of the 3,490 base long glycine decarboxylse cDNA was determined. H-protein cDNA was also cloned by immunoscreening, and this encoded the precursor from of chicken h-protein of 164 amino acid residues within the 840 base long cDNA sequence. Using both cDNA fragments we could analyze the mode of expression of the glycine decarboxylase and h-protein genes.
Glycine decarboxylase mRNA aboundance varied in parallel with the content of the enzyme system in liver, kidney, and brain, which reveal specific activities of the overall reaction at a tatio of 35:11:1. in contrast, heart, spleen, and skeletal muscle mitochondria inactive in the reaction contained significant but small amounts of active H-protein and its mRNAs, whereas glycine decarboxylase and its mRNA were not found, indicationg that tissue-specific distribution of the enzyme system is primarily determined by expression of the hlycine decarboxylase gene. We defined them as the basal expression of the H-protein gene. Excluding the basal expression, relative efficiencies of run-off transcription on the glycine decarboxylase and H-protein genes appeared to be equal and the equimolar level of glycine decarboxylase and H-protein mRNAs are maintaianed in liver, kidney, and brain, irrespective of the difference in the amounts of expression of the genes. It is suggested that the magnitude of the glycine cleavage activity is specified by the coordinate mechanism which resides in regulation for biosynthesis of the components of the glycine cleavage system, except that the glycine decarboxylase gene expression alone is repressed in the cells of mesenchymal origin.

Report

(3 results)
  • 1988 Annual Research Report   Final Research Report Summary
  • 1987 Annual Research Report
  • Research Products

    (22 results)

All Other

All Publications (22 results)

  • [Publications] Hiraga,K.;Kure,S.;Yamamoto,M.;Ishiguro,Y.;Suzuki,T.: Biochem.Biophys.Res.Commun.151. 758-762 (1988)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Kume,A.;Kure,S.;Tada,K.;Hiraga,K.: Biochem.Biophys.Res.Commun.154. 292-297 (1988)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Yamamoto,M.;Hiraga,K.: J.Biol.Chem.Submitted.

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Kure,S.;Kume,A.;Ishiguro,Y.;Hiraga,K.: J.Biol.Chem.Submitted.

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Kume,A.;Sakakibara,T.;Koyata,H.;Ishiguro,Y.;Kure,S.;Tada,K.;Hiraga,K.: J.Biol.Chem.Submitted.

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] 平賀紘一: 蛋白質・核酸・酵素. 33. 526-528 (1988)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Hiraga, K.; Kure, S.; Yamamoto, M.; Ishiguro, Y.; Suzuki, T.: "Cloning of cDNA encoding human H-protein, a constituent of the glycine cleavage system" Biochem. Biophys. Res. Commun.151. 758-762 (1988)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Kume, A.; Kure, S.; Tada, K.; Hiraga, K.: "The impaired expression of glycine decarboxylase in patients with hyperglycinemias" Biochem. Biophys. Res. Commun.154. 292-297 (1988)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Yamamoto, M.; Hiraga, K.: "The Glycine Cleavagy System: Molecular Cloning of cDNA and The Gene Encoding Chicken H-Protein, and Evidence for Multiple Use of The Polyadenylation Consensus to Form The H-Protein mRNAs." J. Biol. Chem.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Kure, S.; Kume, A.; Ishiguro, Y.; Hiraga, K.: "The Glycine Cleavage System: Molecular Cloning of Chicken Glycine Decarboxylase cDNA and The Coupled Expression of The Glycine Decarboxylase and H-Protein Genes in Chicken" J. Biol. Chem.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Kume, A.; Sakakibara, T.; Koyata, H.; Ishiguro, Y.; Kure, S.; Tada, K.;Hiraga, K.: "The Glycine Cleavage System: Molecular Cloning and Structural Analysis of Human Glycine Decarboxylase cDNA, and Molecular Evolution of Glycine Decarboxylase" J. Biol. Chem.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Hiraga,K.;Kure,S.;Yamamoto,M.;Ishiguro,Y.;Suzuki,T.: Biochem.Biophys.Res.Commun.151. 758-762 (1988)

    • Related Report
      1988 Annual Research Report
  • [Publications] Kume,A.;Kure,S.;Tada,K.;Hiraga,K.: Biochem.Biophys.Res.Commun.154. 292-297 (1988)

    • Related Report
      1988 Annual Research Report
  • [Publications] Yamaoto,N.;Hiraga,K.: J.Biol.Chem.

    • Related Report
      1988 Annual Research Report
  • [Publications] Kure,S.;Kume,A.;Ishiguro,Y.;Hiraga,K.: J.Biol.Chem.

    • Related Report
      1988 Annual Research Report
  • [Publications] Kume,A.;Sakakibara,T.;Koyata,H.;Ishiguro,Y.;Kure,S.;Tada,K.;Hiraga,K.: J.Bilo.Chem.

    • Related Report
      1988 Annual Research Report
  • [Publications] 平賀紘一: 蛋白質・核酸・酵素. 33. 526-528 (1988)

    • Related Report
      1988 Annual Research Report
  • [Publications] Koichi Hiraga: Biochem, Biopheys, Res. Commun. (1988)

    • Related Report
      1987 Annual Research Report
  • [Publications] 平賀紘一: 蛋白質・核酸・酵素. (1988)

    • Related Report
      1987 Annual Research Report
  • [Publications] 平賀紘一: 生化学. 59. 977 (1987)

    • Related Report
      1987 Annual Research Report
  • [Publications] 呉繁夫: 生化学. 59. 879 (1987)

    • Related Report
      1987 Annual Research Report
  • [Publications] 石黒義久: 生化学. 59. 902 (1987)

    • Related Report
      1987 Annual Research Report

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Published: 1987-04-01   Modified: 2016-04-21  

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