| Project/Area Number |
62570455
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
Dermatology
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| Research Institution | Nagasaki University |
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Principal Investigator |
SADAMORI Naoki Nagasaki University, School of Medicine, Lecturer, 医学部, 講師 (30039896)
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| Co-Investigator(Kenkyū-buntansha) |
TOKUNAGA Seiji Nagasaki University, School of Medicine, Resident, 医学部, 医員
ITOYAMA Takahiro Nagasaki University, School of Medicine, Resident, 医学部, 医員
SASAGAWA Ippei Nagasaki University, School of Medicine, Resident, 医学部, 医員
NAKAMURA Hideo Nagasaki University, School of Medicine, Resident, 医学部, 医員
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| Project Period (FY) |
1987 – 1989
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| Project Status |
Completed (Fiscal Year 1989)
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| Budget Amount *help |
¥2,100,000 (Direct Cost: ¥2,100,000)
Fiscal Year 1989: ¥400,000 (Direct Cost: ¥400,000)
Fiscal Year 1988: ¥400,000 (Direct Cost: ¥400,000)
Fiscal Year 1987: ¥1,300,000 (Direct Cost: ¥1,300,000)
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| Keywords | Atomic Bomb Survivors / Cultured Skin Cells / Chromosome Abnormalities |
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| Research Abstract |
[Introduction] In this study, to clarify the carcinogenesis of skin cancer in atomic bomb survivors, cytogenetic studies of cultured skin cells were performed.
[Methods] Fresh skin tissue was cultured in CO_2 incubator on MEM medium for 2 to 7 weeks. The cells were allowed to proliferate and at an appropriate time with colcemide for 4 hours to prepare chromosome specimens.
[Results] Structural abnormality was observed in only one out of the total 293 cultured skin cells(0.3%) in non-exposed subjects. On the other hand, in Case 1 exposed at 1250m from the hypocenter without any shielding, 42 out of 220 cultured skin cells(19,1%) showed chromosome abnormalities. In case 2 exposed directly at 1390m from the hypocenter, 16 out of 21 cultured skin cells revealed structural chromosome abnormalities. In Cases 1 and 2, there were several clones with the same abnormal karyotype. In a clone of t(1;20)(p11;q13) in Case 1, breakpoints of 1p11 and 20q13 have been assigned as the loci of proto-oncogenes N-ras and src, respectively. In a clone of t(2;13)(q14;q33) in Case 7, breakpoints of 2q14 and 13q33 have been assigned as the loci of proto-concogenes lca and cbt, respectively. As summary of the results in this study, the frequency of cells with chromosomal abnormalities seems to depend on the distance from the hypocenter and shielding at exposure and not to be based on normal or scar tissues.
[Discussion] In some proximally exposed atomic bomb survivors, several abnormal clones with translocation exist in cultured skin cells. It is of interest to note a close relationship between the breakpoints on these abnormal clones and the loci of proto-oncogenes.
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