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Co-operative Research Study on Bacterial Surface Molecules and Bioresponse Modifying Activities in Host Animals.

Research Project

Project/Area Number 63304036
Research Category

Grant-in-Aid for Co-operative Research (A)

Allocation TypeSingle-year Grants
Research Field 細菌学
Research InstitutionOsaka City University

Principal Investigator

YANO Ikuya  Osaka City University Mekical School, Professor, 医学部, 教授 (60047008)

Co-Investigator(Kenkyū-buntansha) AMAKO Kazunobu  Kyushu University Medical School, Professor, 医学部, 教授 (20078752)
KAWATA Tomio  Tokushima University Medical School, Professor, 医学部, 教授 (60035368)
KANEMASA Yasuhiro  Okayama University Medical School, Professor, 医学部, 教授 (80033059)
KATO Keijiro  Okayama University Dental School, Professor, 歯学部, 教授 (50028718)
NAKAE Taiji  Tokai University Medical School, Professor, 医学部, 教授 (50102851)
三輪谷 俊夫  大阪大学, 微生物病研究所, 教授 (60029759)
横田 健  順天堂大学, 医学部, 教授 (10052953)
Project Period (FY) 1988 – 1989
Project Status Completed (Fiscal Year 1990)
Budget Amount *help
¥12,400,000 (Direct Cost: ¥12,400,000)
Fiscal Year 1989: ¥6,100,000 (Direct Cost: ¥6,100,000)
Fiscal Year 1988: ¥6,300,000 (Direct Cost: ¥6,300,000)
KeywordsOuter membrane permeability / Opportunistic pathogen / Mycoloyl glycolipids / Cord factor / Lipopolysaccharides / Lipoteichoic acids / Enterotoxigenic E. coli / Listerial infection / 細菌細胞壁表層物質 / 感染要員 / 生体防禦調節物質 / 細菌表層蛋白質(外膜蛋白質) / 細胞壁両親媒性物質 / 細胞壁脂質 / 細菌細胞構成的病原因子 / 細菌表層タン白質 / 細胞膜脂質 / テイコ酸 / 両親媒性物質 / リポ多糖 / 病原細菌定着因子 / 薬剤透過障害因子 / 感染防御抗原
Research Abstract

According to the initial plannings, we have investigated on the structure-bioresponse modifying activities of the surface molecules of gram-positive, gram-negative and acid-fast bacteria. In gram-negative bacteria, porin subunit structure which is related to the permeability of the outer membrane was investigated and the properties especially of Pseudomonas and Alcaligenes species were demonstrated by Nakae et al. In acid-fast bacteria, the fine molecular structure of mycolic acids and mycoloyl glycolipids such as cord factor. Since it has been known that these characteristic glycolipids possessed unique activities, the essential structure for immunomodifying activities was investigated in host animals. Related to this acid-fast bacteria, a new amphipatic fraction resembling to gram-negative LPS was isolated by Tamura. This fraction showed similar activities to the gram-negtive endotoxin, but less toxic. Since we have no information available about such gram-positive endotoxin, the structure analysis and mechanism of action on host animals would be greatly expected in future. Also recently, the heterogeneity of structure and activity of gram-negative LPS. Takada et al investigated on various less toxic gram-negative LPS isolated from anaerobic bacteria. In gram-positive bacteria, teichoic acids (membrane- and wall teichoic acids) are one of the characteristic components. Kato et al reported the isolation and characterization of less toxic LTA which can generate a non-toxic TNF. Activities of LTA on host animals should be more investigated in future. Exotoxin production and its secretion from the bacterial cells were also investigated especially with enterotoxigenic E. Coli and Vibrio parahaemolyticus. Fine structure analysis of gram-poam-positive bacteria was investigated by using electronmicroscopy by Amako and Kawata et al.
We hope further development and progress of all the above projects in future and financial support from GRANT-IN-AID FOR SCIENTIFIC RESEARCH.

Report

(3 results)
  • 1990 Final Research Report Summary
  • 1989 Annual Research Report
  • 1988 Annual Research Report
  • Research Products

    (43 results)

All Other

All Publications (43 results)

  • [Publications] Yoshihara,E.and Nakae,T.: "Purification of porins in the outer membrane of Pseudomonas aeruginosa that form small cliffusion pores" The Journal of Biological Chemistry. 264. 6297-6301 (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Gotoh,N.et al.: "Role of protein F in maintaining structural integrity of the Pseudomonas aeruginosa outer membrane" Journal of Bacteriology. 171. 983-990 (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Takada,H.et al.: "Structural reguirements of lipid A species activation of clotling enzymes from the horseshoe crab,and human complement cascade" European Journal of Biochemistry. 175. 573-580 (1988)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Usami,It.et al.: "Antitumor effects of streptococcal lipoteichoic acids on Meth A tibrosarcoma" British Journal of Caucer. 57. 70-73 (1988)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Masuda,N.et al.: "Characterization and reassembly of a regular array in the cell wall of Clostridium difficiel GAI 4131." Microbiology and Immunology. 33. 287-298 (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Takade,A.et al.: "The SubstitutionーFixation of Staphylococcus aureus" Journal of Electron Microseopy. 37. 215-217 (1988)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Serushago,B.A.etal.: "Role antibodies against outer ーmembsane proteins in murial resistance to infection with encapsulated Klebsiella preumonide" Journal of General Microbiology. 135. 2259-2268 (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Seki,K.et al.: "A simple method for observation of phagocytosis on bacterial thinー lajer" Microbiology and immunology. 33. 81-85 (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Eya,S.et al.: "Overproduction of truncated subunit a of H^<【plus-minus】>ATPase causes growth inhibition of Escherichia coli" Journal of Bacteriology. 171. 6853-6858 (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Matsunaga,I et al.: "Mycolyl glyco lipids stimulate macrophages to release a chemotactic factor" FEMS Microbiology Letters. 67. 49-54 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Takada,H.etal.: "Bacterial and tungal products.In M.A.Bray and J.Morlay(eds.)Handbook of Experimental Pharmacology,vol,85.The Pharmacology of Lymphocytes" SpringerーVerlag,Berlin, 555-575 (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Yano,I.etal.: "Biology of Actinoiry cltes '88(ed.by Y.Okami etal.)" Japan Scientific Societies press,Tokyo., 468-477 (1988)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Takada, H. et al.: "Structural requirement of lipid A species in activating of clotting enzymes from the horse shoe crab, and the human complement cascade" Eur. J. Biochem. 175. 573-580 (1988)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Usami, H. et al.: "Antitumor effect of streptococcal lipoteichoic acids on Meth A fibrosarcoma" British J. Cancer. 57. 70-73 (1988)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Takade, A. et al.: "The substitution-fixation of Staphylococcus aureus" J. of Electron Microscopy. 37. 215-217 (1988)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Yano, I. et al.: "Molecular structure and immunopharmacological activities of the new glycolipids containing mycolic acids in actinomycetales. in Biology of Actinomycetes '88 (edited by Okami, Y. et al.)" Biology of Actinomycetes. 88.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Yoshihara, E. and Nakae, T.: "Purification of porins in the outermembrane of Pseudomonas aeruginosa that form small diffusion pores." J. Biol. Chem. 264. 6297-6301 (1989)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Gotoh, N. et al.: "Role of protein F in maintaining structural integrity of the Pseudomonas aeruginosa outer membrane" J. of Bacteriol. 171. 983-990 (1989)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Masuda, N. et al.: "Characterization and reassembly of a regular array in the cell wall of Clostridium difficile GAI 4131" Microbiology and Immunology. 33. 287-298 (1989)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Seki, K. et al.: "A simple method for observation of phagocytosis on bacterial thin-layer" Microbiology and Immunology. 33. 81-85 (1989)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Eya, S. et al.: "Overproduction of truncated subunit a of H -ATPase causes growth inhibition of Escherichia coli" J. of Bacteriol. 171. 6853-6858 (1989)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Matsunaga, I. et al.: "Mycoloyl glycolipids stimulate macrophages to release a chemotactic factor" FEMS Microbiology Letters. 67. 49-54 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Ikawa, H. et al.: "Rapid identification of serotypes of Mycobacterium avium-M. intracellulare complex by using infected swine sera and reference antigenic glycolipids" J. Clin. Microbiol.27. 2552-2558 (1989)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Kawai, Y. et al.: "Various Kinds of lipoamono acids including a novel serine-containing lipid in an opportunistic pathogen Flavobacterium" Eur. J. Biochem. 171. 73-80 (1988)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Yoshihara,E. and Nakae,T.: "Purification of porins in the outer membrane of Pseudomonas aeruginosa that from small diffusion pores" The Journal of Biological Chemistry. 264. 6297-6301 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] Gotoh,N.et al.: "Role of protein F in maintaining structural integrity of the Pseudomonas aeruginosa auter membrane" Journal of Bacteriology. 171. 983-990 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] Takada,H.et al.: "Structural requirements of lipid A sphecies in activation of clotting enzymes from the horseshoe crab,and the human complement cascade" European Journal of Biochemistry. 175. 573-580 (1988)

    • Related Report
      1989 Annual Research Report
  • [Publications] Usami,H.et al.: "Autitumor effects of streptococcal lipoteichoic acids on Meth A tibrosarcoma" British Journal of Caucer. 57. 70-73 (1988)

    • Related Report
      1989 Annual Research Report
  • [Publications] Masuda,N.et al.: "Characterization and reassembly of a regular array in the cell wall of Clostridium difficill GAI4131." Microbiology and Immunology. 33. 287-298 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] Takade,A.et al.: "The Substitution-Fixation of Staphylococcus aureus" Journal of Electron Microseopy. 37. 215-217 (1988)

    • Related Report
      1989 Annual Research Report
  • [Publications] Serushago,B.A.et al.: "Role of autibodies against outer membsaul proteins in wuriae resistance to infection with encapsulated Klebsiella preumonide" Journal of General Microbiology. 135. 2259-2268 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] Seki,K.et al.: "A Simple method for observation of phagocytosis on bacterial thin-layer" Microbiology and immunology. 33. 81-85 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] Eya,S.et al.: "Overproduction of truncated subunit a of H^+ ATPhase causes growth inhibition of Escherichia coli" Journal of Bacteriology. 171. 6853-6858 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] Matsunaga,I et al.: "Mycolyl glycolipids stimulate macrophages to release a chemotactic factor" FEMS Microbiology Letters. 67. 49-54 (1990)

    • Related Report
      1989 Annual Research Report
  • [Publications] Takada,H.et al.: "Bacterial and fungal products.In M.A Bray and J.Morley Hardbook of Experimental Pharmacology,Vol.85.The Pharmacology of Lymphocytes" Springer-Verlag,Berlin, 555-575 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] Yano,I.et al.: "Biology of Actinomycetes '88(ed.by Y.Okami et al.)" Japan Scientific Societies Press,Tokyo., 468-477 (1988)

    • Related Report
      1989 Annual Research Report
  • [Publications] Masuda,K.;Itoh,M.;Kawata,T.: Microfiol.Immunol.;33(4). 33(4). (1989)

    • Related Report
      1988 Annual Research Report
  • [Publications] 平松啓一、鈴木映子、高山弘美、横田健: 日本細菌学雑誌. 44(1). 125 (1989)

    • Related Report
      1988 Annual Research Report
  • [Publications] 関啓子、小笠原正美、櫻田純次、村井美代、益田昭吾: 日本細菌学雑誌. 44(1). 318 (1989)

    • Related Report
      1988 Annual Research Report
  • [Publications] 本田武司、小口富明、Wetprasit,N.、有田美知子、三輪谷俊夫: 日本細菌学雑誌. 44(1). 331 (1989)

    • Related Report
      1988 Annual Research Report
  • [Publications] 石井純子、佐竹幸子、中江太治: 日本細菌学雑誌. 44(1). 471 (1989)

    • Related Report
      1988 Annual Research Report
  • [Publications] Yokoi,F.;Sugimoto,N.:Kato,Y.;Kaueda,K.;Shiro,O.;Yano,I.: Yakugaku Zasshi. 109(1). 46-51 (1989)

    • Related Report
      1988 Annual Research Report
  • [Publications] Yano,I.;Oka,S.;Natsuhara,Y.;Kato,Y.;Tomiyasu,I.;Kaneda,K.: "Molecular structure and immunopharmacological activities of the new glycolipids containing mycolic acids in Actinomycetales in Biology of Actinomycetes 188" Japan Scientifie Societi Press, 469-477 (1988)

    • Related Report
      1988 Annual Research Report

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Published: 1988-04-01   Modified: 2016-04-21  

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