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Microbial control of growth, differentiation, metamorphosis and settlement of marine organisms.

Research Project

Project/Area Number 63440015
Research Category

Grant-in-Aid for General Scientific Research (A)

Allocation TypeSingle-year Grants
Research Field General fisheries
Research InstitutionKyoto University

Principal Investigator

ISHIDA Yuzaburo  Kyoto Univ. Fisheries Professor, 農学部, 教授 (20026488)

Co-Investigator(Kenkyū-buntansha) TANAKA Masaru  Kyoto Univ. Fisheries Assoc. Professor, 農学部, 助教授 (20155170)
SAKAGUCHI Morihiko  Kyoto Univ. Fisheries Professor, 農学部, 教授 (00027187)
YOSHINAGA Ikuo  Kyoto Univ. Fisheries Research Assoc., 農学部, 助手 (40230776)
SAKO Yoshihiko  Kyoto Univ. Fisheries Research Assoc., 農学部, 助手 (60153970)
UCHIDA Aritsune  Kyoto Univ. Fisheries Assoc. Professor, 農学部, 助教授 (50027190)
深見 公雄  京都大学, 農学部, 助手 (30181241)
Project Period (FY) 1988 – 1991
Project Status Completed (Fiscal Year 1991)
Budget Amount *help
¥27,000,000 (Direct Cost: ¥27,000,000)
Fiscal Year 1991: ¥2,500,000 (Direct Cost: ¥2,500,000)
Fiscal Year 1990: ¥3,000,000 (Direct Cost: ¥3,000,000)
Fiscal Year 1989: ¥4,100,000 (Direct Cost: ¥4,100,000)
Fiscal Year 1988: ¥17,400,000 (Direct Cost: ¥17,400,000)
KeywordsThyroid / Japanese flounder / Settlement and metamorphosis / Acinetobacter SS6-2 / Dimethylsulfoniopropionate / Methionine decarboxylase / Plasmid pBI121 / Ulva pertusa / 異体類の変態機構 / コルチゾル / ヒラメ稚魚 / ジメチル・スルフォプロピオン酸(DMSP) / メチオニン脱炭酸酵素 / 遺伝子導入 / プラスミッドpBI121 / ヒラメ / 変態・着定 / ジメチル・β・プロピオテチン / 微細藻類 / 成長促進 / 稚仔魚 / 渦鞭毛藻 / サイロキシン / ジメチル・β・プロピオテチン(DMPT) / Navicula sp.
Research Abstract

In this study we attempted to make clear a mechanism of metamorphosis and settlement, and to search and isolate microalgae and bacteria participating in these phenomena, and to identify' their bioactive substances. The results obtained are as follows ;
1. The endocine system ii of fundamental importance in controlling development and growth of fishes. Thyroid and pituitary glands differentiate during the final phase of yolk absorption in pelagic -egg species. Interrenal tissues appear later than both glands, around 2 weeks after hatching in Japanese flounder. Immumehistochemical techniques revealed production of GH and PRL prior to the time when the pituitary is histologically detectable. Tissue TH concentrations were at higher levels at fertilization, thereafter markedly reduced with embryonic development. They gradually increased with postlawal growth and development, and appreciably increased during metamorphosis in flatfishes. In some flatfishes, tissue concentration of cortisol als … More o exhibited a prominent surge, but the timing was significantly earlier than that of TH. These hormone level dynamics appeared to be intimately related to body structual, behavioral and ecological changes.
2. Many marine microorganisms which stimulate settlement of flounder (Paralichthys obivaceus) larvae were surveyed. Marine Acinetobacter SS6-2 and Raphidophyta Chattonella antiqua a86 were effective on the settlement. The active substances were found in acetone or ethanol insoluble fraction.
3. Dimethylsulfoniopropionate (DMSP) is the precursor of dimethylsulfide (DMS) which is the most abundant volatile sulfur compound in seawater. Various species of marine macroalgae (especially Rhodopohyta) and microalgae (especially Dinophyta and Haptophyta) contain DMSP. ' DMSP has promoting effect on growth, feeding and thrust power of several fishes. The biosynthetic pathway of DMSP from methionine in Crypthecodinium cohnii (Dinophyta). Methionine is first decarboxylated by methionine decarboxylase which requires pyridoxyal phsphate. We are now trying to purify this enzyme.
4. Polyethyleneglycol method was applied to tranformation of the linearized plasmid pBI121 which contains the neomycin phosophotransferease II (NPTII) gene and beta-gluconidase (GUS) gene into the cell wall-less mutant of Chiamydomonas reinhardtii. This transformation procedure is very simple. Cells tranformed to kanamycin resistance were obtained. Southem blots of DNA from the transformant demonstrate the integration of the PNT II gene but GUS gene. We are now trying to make transgenic thalli of Ulva pertusa which can produce the growth hormone of tuna. Less

Report

(5 results)
  • 1991 Annual Research Report   Final Research Report Summary
  • 1990 Annual Research Report
  • 1989 Annual Research Report
  • 1988 Annual Research Report
  • Research Products

    (50 results)

All Other

All Publications (50 results)

  • [Publications] A.Uchida Stable protoplast isolation and its regeration into thallu: "s of the marine green alga Ulva pertusa." Nippon Suisan Gakkaishi. 58. 153-157 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] I.Yoshinaga: "Comparison of DNA and protein synthesis rates of bacterial assemblages between coral reef waters and pelagic waters in tropical ocean." Marine Ecology Progress Series. 27. 167-174 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] C.Chantanachookhin: "Comparative study of the ontogeny of the lymphoidorgans in three species of marine fish." Aquaculture. 99. 143-155 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] M.Tanaka: "A thyroxine surge during development of black sea bream larvae and its ecological implication in inshore migration." Nippon Suisan Gakkaishi. 57. 1827-1832 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] K.Nakajima: "Effect of a feeding attractant Dimethyl-β-propiothetin,on growth of marine fish." Nippon Suisan Gakkaishi. 56. 1151-1154 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] S.Sawayama: "Bacterial inhibitor for the mating reaction in Chlamydomonas reinhardtii and Alexandrium catenella." Nippon Suisan Gakkaishi. 56. 1847-1852 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] 石田 祐三郎: "海洋微生物とバイオテクノロジ-" 技報堂出版, 370 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] 内田 有恒: "微生物の生態ー微生物のガス代謝と地球環境ーDimethylsulphideの分布と発生機構" 学会出版センタ-, (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Kenji Nakajima, Aritsune Uchida, and Yuzaburo Ishida: "A new feeding stimulant, dimethyl-beta-propiothetin, for freshwater fish" Nippon Suisan Gakkaishi (Bull. Japan Soc. Sci. Fish). 55. 689-695 (1989)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Kenji Nakajima, Aritsune Uchida, and Yuzaburo Ishida: "Effects of heterocyclic sulfur-containing compounds on the striking response of goldfish" Memory of Koshien University. No. 16. 7-10 (1989)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Kenji Nakajima, Aritsune Uchida, and Yuzaburo Ishida: "Effect of supplemental dietary feeding attractant, dimethyl-beta-propiothetin, on growth of goldfish" Nippon Suisan Gakkaishi (Bull. Japan Soc. Sci. Fish). 56. 1291 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] K. Fukami, Y. Sakami, Y. Ishida, and N. Tanaka: "Effect of bacterial film on the growth of the attached diatom, Nitzchia sp. ." Topics in Marine Biotechnology. Fuji Technology Press Ltd. 415-418 (1989)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Kenji Nakajima, Aritsune Uchida, and Yuzaburo Ishida: "A feeding attractant dimethyl-beta-propiothetin, on growth of marine fish." Nippon Suisan Gakkaishi. 56. 1151-1154 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Y. Ishida, Y. Ogihara, and S. Okabe: "Effects of a crude extract of a marine dinoflagellate, containing dimethyl-beta -propiothetin, on HCL-ethanol-induced gastic lesions and gastric secretion in rats." Japan J. Pharmacol. 54. 333-338 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Shingo Hiroishi, Nobuyasu Hirooka, Aritsune Uchida, and Yuzaburo Ishida: "Isolation of marine bacterium which produces new vasodilator" Nippon Suisan Gakkaishi. 57, (9). 1707-1709 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Masaru Tanaka, Ryo Kimura Masatomo Tagawa, and Tetsuya Hirano: "A thyroxine surge during development of black sea bream larvae and its ecological implication in inshore migration." Nippon Suisan Gakkaishi. 57, (10). 1827-1832 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Ryo Kimura and Masaru Tanaka: "Prolactin production during larval and early juvenile periods of euryhaline marine fish, black sea bream" Nippon Suisan Gakkaishi. 57, (10). 1833-1837 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Caiyuth Chantanachookhin, Tadahisa Seikai, and Masaru Tanaka: "Comparative study of the ontogeny of the lyphoid organs in three species of marine fish." Aquaculture. 99. 143-155 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Aritune Uchida, Takeshi Yoshikawa, Yuzaburo Ishida, and Naotsune Saga: "High yields of protoplasts isolation and their regeneration into thalli" Nippon suisan Gakkaishi. 58(1). 153-157 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] A.Uchida: "Stable protoplast isolation and its regeneration into thallus of the marine green alga Ulva pertusa." Nippon Suisan Gakkaishi. 58. 153-157 (1992)

    • Related Report
      1991 Annual Research Report
  • [Publications] K.Nagasaki: "A monoclonal antibody which recognizes the cell surface of red tide alga Gymnodinium nagasakiense." Nippon Suisan Gakkaishi. 57. 1211-1214 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] Y.Sako: "Isozyme analysis of mating populations of Gonium pectorale〔chlorphyta〕" Journal of Phycology. 27. 309-315 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] I.Yoshinaga: "Comparison of DNA and protein synthesis rates of bacterial assemblages between coral reef waters and pelagic waters in tropical ocean." Marine Ecology Progress Series. 76. 167-174 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] C.Chantanachookhin: "Comparative study of the ontogeny of the lymphoidorgans in three species of marine fish." Aquaculture. 99. 143-155 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] M.Tanaka: "A thyroxine surge during development of black sea bream larvae and its ecological implication in inshore migration." Nippon Suisan Gakkaishi. 57. 1827-1832 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] 石田 祐三郎: "海洋微生物とバイオテクノロジ-" 技報堂出版, 370 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] 左子 芳彦: "「微生物の生態19」ー微生物生態学の新たな展開と手法ーアイソザイム,モノクロ-ナル抗体法による微細藻の識別" 学会出版センタ-, (1992)

    • Related Report
      1991 Annual Research Report
  • [Publications] Sako,Y.,C.H.Kim H.Ninomiya,M.Adachi and Y.Ishida: "Isozyme and cross analysis of mating populations on the Alexandrium catenella/tamarense species complex." Toxic Marine Phytoplankton. 4. 320-323 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] Nishio,T.and Y.Ishida: "Distribution of siderophoreーproducing bacteria in the sediments of a small lagoon by Lake Biwa." Nippon Suisan Gakkaishi. 56. 655-661 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] Nishio,T.and Y.Ishida: "Production of dihydroxamate siderophore alcaligin by Alcaligenes xylosoxidans subsp.xylosoxidans." Agricultural and Biological Chemistry. 54. 1837-1839 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] Nakajima,K.,A.Uchida,and Y.Ishida: "Effect of a feeding attractant Dimethylーβーpropiothetin,on growth of marine fish." Nippon Suisan Gakkaishi. 56. 1151-1154 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] Fukami,K.,S.Ohara and Y.Ishida: "Effect of extracellular organic carbon (EOC) from phytoplankton on the community structure of oligotrophic bacteria." Arch.Hydrobiol.Beih.,. 34. 43-47 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] Sawayama,S.,Y.Sako and Y.Ishida: "Bacterial inhibitor for the mating reaction in Chlamydomonas reinhardtii and Alexandrium catenella." Nippon Suisan Gakkaishi. 56. 1847-1852 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] 石田 祐三郎: "水産学シリ-ズ79 細菌および微細藻類由来の生物制御物質" 恒星社厚生閣, 109 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] 石田 祐三郎: "日本の赤潮生物" 内田老鶴圃, 407 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] A.Uchida: "The application of monoclonal antibodies to an identification of Chattonella marina and Chattonella antiqua." Nippon Suisan Gakkaishi. 55. 721-725 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] K.Nakajima: "Effects of heterocyclic sulferーcontaining compounds on the striking response of goldfish." 甲子園大学紀要. 6. 7-10 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] M.Eguchi: "Oligotrophic properties of heterotrophic bacteria and in situ heterotrophic activity in pelagic seawaters." FEM Microbiol.Ecol. 73. 23-30 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] M.Tanaka: "Lunarーphased immigration and settlement of metamorphosing Japanese flounder larvae into the nearshore nursery ground." Rapp.P.ーv Reun.Cons.int.Exp.Mer.191. 303-310 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] M.Tanaka: "IMMIGRATION,SETTLEMENT AND MORTALITY OF FLOUNDER (PARALICHTHYS OLIVACEUS)LARVAE AND JUVENILES IN A NURSERY GROUND,SHIJIKI BAY,JAPAN." Neth.J.Sea Res.24. 57-67 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] J.B.Tanangonan: "Changes in Tissue Thyroxine Level of Metamorphosing Japanese Flounder Paralichthys olivaceus Reared at Different Temperatures" Nippon Suisan Gakkaishi. 55. 485-490 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] Y.Ishida: "水産学シリ-ズ77 魚介類の生息環境と着臭" 恒星社厚生閣, 125 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] Y.Ishida: "RECENT ADVANCES IN MICROBIAL ECOLOGY" Japan Scientific Societies Press, 724 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] Kenji Nakajima: Nippon Suisan Gakkaishi. 55. (1989)

    • Related Report
      1988 Annual Research Report
  • [Publications] Kenji Nakajima: Nippon Suisan Gakkaishi. 55. (1989)

    • Related Report
      1988 Annual Research Report
  • [Publications] 田中克: 水産土木. 24. 33-44 (1988)

    • Related Report
      1988 Annual Research Report
  • [Publications] 後藤常夫: 日本水産学会誌. 55. 9-16 (1989)

    • Related Report
      1988 Annual Research Report
  • [Publications] 藤井徹生: 日本水産学会誌. 55. 17-24 (1989)

    • Related Report
      1988 Annual Research Report
  • [Publications] J.B.Tanangonan: Nippon Suisan Gakkaishi. 55. (1989)

    • Related Report
      1988 Annual Research Report
  • [Publications] M.Tanaka: Neth.J.Sea Res.23. (1989)

    • Related Report
      1988 Annual Research Report

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Published: 1988-04-01   Modified: 2016-04-21  

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