| Budget Amount *help |
¥2,200,000 (Direct Cost: ¥2,200,000)
Fiscal Year 1989: ¥900,000 (Direct Cost: ¥900,000)
Fiscal Year 1988: ¥1,300,000 (Direct Cost: ¥1,300,000)
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| Research Abstract |
Abstract. In order to examine the effects of increased intracellular Ca^<2+> level on the membrane electrical activity, caffeine, the agent which facilitates Ca^<2+> release from the sarcoplasmic reticulum, was applied to single ventricular cells of the guinea-pig, under condition of the whole cell recording. Caffeine (10 - 25 mM) induced a burst of pulse-like small inward currents. The amplitude of the pulse-like inward currents, which appeared to have a unit magnitude, decreased as the membrane was depolarized, and the reversal potential was estimated to be near 0 mV. Thus, the responsible ion channel may have a unit conductance of 250 - 300 pS. The pulse-like activity persisted when external NaCl was replaced with LiCl, but it disappeared in Tris-Cl solution. Large depolarizations producing sustained cell shortening or intracellular Ca^<2+>-injection enhanced the pulse-like events, suggesting a role of intracellular Ca^<2+> in this phenomenon. Ni^<2+> ions partially inhibited the pulse-like activity. Thus, the ventricular cells appeared to have Ca^<2+>-mediated cation channels with a large conductance, but possible involvement of a specific action of caffeine in the channel activation was not excluded.
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