Studies of Neuronal Properties in the Reaggregate Cultures of Rat Retinane
| Project/Area Number |
63570064
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
Neurophysiology and muscle physiology
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| Research Institution | Okazaki National Research Institutes (1989-1990)
Saitama Medical University (1988) |
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Principal Investigator |
AKAGAWA Kimio National Institute for Physiological Sciences, Laboratory of Neurochemistry, Associate Professor, 生理学研究所, 助教授 (80129303)
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| Co-Investigator(Kenkyū-buntansha) |
SHIRAO Tomoaki National Institute for Physiological Sciences, Laboratory of Neurochemistry, Res, 生理学研究所, 助手 (20171043)
吉村 和法 埼玉医科大学, 医学部, 助手 (20158497)
田中 早苗 埼玉医科大学, 生理学, 助手
鈴木 勝 埼玉医科大学, 生理学, 助手 (40095378)
植村 慶一 埼玉医科大学, 生理学, 教授 (90049792)
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| Project Period (FY) |
1988 – 1990
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| Project Status |
Completed (Fiscal Year 1990)
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| Budget Amount *help |
¥2,100,000 (Direct Cost: ¥2,100,000)
Fiscal Year 1990: ¥400,000 (Direct Cost: ¥400,000)
Fiscal Year 1989: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 1988: ¥1,200,000 (Direct Cost: ¥1,200,000)
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| Keywords | Rat retina / Reaggregate culture / Layer formation / Light response / アマクリン細胞 / パッチクランプ法 / 層構造 |
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| Research Abstract |
Retinal reaggregate cultures were established from new born rats. All the experiments were carried out 10-15 days after plating.
Histological studies revealed that selective layer formation were reconstituted in the reaggegates. Since these histological properties resembled those in vivo, electro-physiological experiments were carried out in order to study the possible presence of neural net works in vitro, which might be rudi
Mentary for retinal functions. It was possible to establish cell attach configuration on the cells in the outermost layer. Under a dark condition, about one fifth of the cells in the outermost layer showed spontaneous discharges. Interestingly, a few neurons increased the frequency of discharges when light stimuli was given to the whole reaggregate. The proportion of the cells with such light-response like activity was 1.6%. Since it was already reported by an immunohistological study that most cells in the outermost layer were amacrine cells and the photoreceptors were exclusively localized in the central region, these results indicated that functional connections were reconstituted between the photoreceptors and the amacrine cells in the reaggregate cultures. These results also demonstrated that the reaggregate cultures could provide interesting models to study the development of neural networks in vitro.
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Report
(4 results)
Research Products
(5 results)
