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HLA-DR Antigen Positive Cells in the RA Synovium. -Their Significant Role Concerning in the Continuation of Chronic Inflammation of RA.

Research Project

Project/Area Number 63570138
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field Human pathology
Research InstitutionTOHOKU UNIVERSITY

Principal Investigator

MURAKAMI Kazuhiro  Tohoku University Hospital, Research Associate, 医学部附属病院, 助手 (90190876)

Co-Investigator(Kenkyū-buntansha) KATOH Mistuyasu  Tohoku University School of Medicine, Research Associate, 医学部, 助手 (20194855)
Project Period (FY) 1988 – 1990
Project Status Completed (Fiscal Year 1990)
Budget Amount *help
¥2,200,000 (Direct Cost: ¥2,200,000)
Fiscal Year 1990: ¥400,000 (Direct Cost: ¥400,000)
Fiscal Year 1989: ¥400,000 (Direct Cost: ¥400,000)
Fiscal Year 1988: ¥1,400,000 (Direct Cost: ¥1,400,000)
KeywordsRheumatoid arthritis / Synovial cell / HLA-DR antigen / 清膜細胞 / リウマトイド結節 / HLAーDR陽性細胞 / HLA / DR抗原 / 螢光抗体法
Research Abstract

1 Characteristics of RA synovial cells The sublining D cells which were remarkably proliferating in the rheumatoid synovial membrane were divided into two type cells, D(F) cells and D(M) cells immunohistochemically. In culture, D(F) cells showed high proliferative activity and easily transformed their morphological and functional character under the effects of variable cytokines. On the other hand, D(M) cells proliferated very slowly, and they contiguously and strongly expressed HLA-DR antigen on their surface. These findings suggest that D(F) cell plays an important role in formation of histopathological variety of RA synovitis and D(M) cell is largely concerned in the immunological activity of RA. 2 Histopathological study of early stage of synovial lesion We investigated the early stage of RA synovitis immunohistochemically and from this study it become clear that in very early of RA, HLA-DR positive cells were found in pericapillary region and following them T lymphocytes appeared and B lymphocytes appeared to aggregate in later. 3 The effects of drugs on RA synovitis Some patients with early stage RA showed improvement of condition of disease after treatment with immunomodulating drug (CCA), but no apparent qualitative and quantitative changes of HLA-DR positive cells in synovium were observed. These findings suggest the complexity of cellular interaction in development of RA synovitis. 4 Comparison of synovial cells with cells in rheumatoid nodule The characteristics of majority of cells in rheumatoid nodule were quite similar to that of D cells in rheumatoid synovitis. Clinically rheumatoid synovitis is characterized by its progressive and destructive nature in contrast to rheumatoid nodule. Histopathologically in the rheumatoid nodule the lymphocyte infiltration was less conspicuous. These findings suggest that lymphocyte infiltration is essential for the continuation of chronic destructive inflammation of RA synovitis.

Report

(4 results)
  • 1990 Annual Research Report   Final Research Report Summary
  • 1989 Annual Research Report
  • 1988 Annual Research Report
  • Research Products

    (36 results)

All Other

All Publications (36 results)

  • [Publications] 村上 一宏: "未石灰化骨器貭に対するレクチン染色の応用" 日本公衆衛生協会 環境保健レポ-ト. 56. 132-135 (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Sawai Takashi: "Stromelysin synthesizing cells in the synovial tissue of rheumatoid arthritis demonstrated by in situ hybridization and immunohistochemical methods" Tohoku J.Exp.Med.

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] 村上 一宏: "リウマトイド結節構成細胞の解折 ー滑膜細胞との比較ー" 炎症. 10. 394-354 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] 村上 一宏: "ATLA抗体陽性の関節炎の2症例 ー免疫組織学的、電顕的観察ー" 最新医学. 45. 194-195 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] 沢井 高志: "慢性関節リウマチ滑膜初期病変の免疫組識学的検討 ーモノクロナル抗体を用いた炎症性細胞の定性ならびに定量的解折ー" リウマチ. 30. 247-254 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] 沢井 高志: "慢性関節リウマチ最近の話題 ーMetalloproteinageとマトリックスの観点からー" 病理と臨床. 81. 1478-1484 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] 沢井 高志: "滑膜細胞 ーサイトカインの滑膜細胞に与える影響ー" 関節外科. 10. 39-44 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] 沢井 高志: "初期慢性関節リウマチ患者に対するLobenzarit disodium(CCA)投与前後の滑膜組織の免疫組織学的検討" 炎症.

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] 沢井 高志: "滑膜細胞とTリンパ球の関係 ーリンフォカイン,モノカインの培養滑膜細胞におよぼす影響ー" リウマチ.

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Murakami Kazuhiro: "Morphological and functional characteristics of cultured human synovial cells obtained from rheumatoid synovium"

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] 沢井 高志(分担執筆): "リウマチ学" 同文書院(東京), (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Murakami K, et al.: "The application of lectin staining for uncalcified bone matrix" Summary of report No56 (Japan Public Health Association). 132-135 (1989)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Sawai T, Murakami K, et al.: "Stromelysin synthesizing cells in the synovial tissue of rheumatoid arthritis demonstrated by in situ hybridization and immunohistochemical methods" Tohoku J. Exp. Med. 160. 285-286 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Murakami K, et al.: "Phenotypic and functional characteristics of cells in rheumatoid nodule -Comparison with synovial cells-" J. J. Inf. 10. 394-354 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Murakami K, et al.: "Two cases of ATLA positive arthritis-immunohistochemical and electoronmicroscopical observation-" The Saishin Igaku. 45. 194-195 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Sawai T, Ohyama A, Murakami K, et al.: "Qualitative and quantitative studies of synovial lesions in early rheumatoid arthritis demonstrated by immunohistochemistry with monoclonal antibodies" Rheumati. 30. 247-254 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Sawai T, Muradami K, et al.: "Recent topics of rheumatoid arthritis -a relationship between matrix and its degrading enzyme, metalloproteinase" Pathology and Clinical Medicine. 87. 1478-1484 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Sawai T, Murakami K, et al.: "Effect of cytokines on synovial cells in rheumatoid arthritis" Journal of Joint Surgery. 10. 39-44 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Sawai T, Ohyama A, Rikimaru A, Murakami K, et al.: "Immunohistochemical evaluation of the effect of a newly developed immunomodulating drug (CCA) to the synovial tissue of patients in the early stage of rheumatoid arthritis (RA)" J. J. Inf.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Sawai T, Murakami K, et al.: "Effects of cytokines on the morphological and functional characters of cultured synovial cells" Rheumati.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Murkami K, et al.: "Morphological and functional characteristics of cultured human synovial cells obtained from rheumatoid synovium"

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] 村上 一宏: "リウマトイド結節構成細胞の解析ー滑膜細胞との比較ー" 炎症. 10. 349-354 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] 村上 一宏: "ATLA抗体陽性の関節炎の2症例ー免疫組織学的・電顕的観察ー" 最新医学. 45. 194-195 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] 沢井 高志: "慢性関節リウマチ滑膜初期病変の免疫組織学的検討ーモノクロナル抗体を用いた炎症性細胞の定性ならびに定量的解析ー" リウマチ. 30. 247-254 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] 沢井 高志: "慢性関節リウマチ最近の話題ーMetalloproteinaseとマトリックスの観点からー" 病理と臨床. 8. 1478-1484 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] Takashi Sawai: "Stromelysin synthesizing cells in the synovial tissue of rheumatoid arthiritis demonstrated by in situ hybridization and immunohistochemical methods" Tohoku J.Exp.Med. 160. 285-286 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] Sawai T: "Stromelysin synthesizing cells in the synovial tisues of rheuamtoid arlhritis demonstrated by in situ hybridization and immundristochem-ical methods." Tohoku J.Exp.Mecl.

    • Related Report
      1989 Annual Research Report
  • [Publications] 沢井高志: "初期慢性関節リウマチ患者の滑膜組織に対するCCA投与の影響-モノクロナル抗体を用いた投与前後の免疫組織学的比較-。" リウマチ.

    • Related Report
      1989 Annual Research Report
  • [Publications] 沢井高志: "慢性関節リウマチ滑膜初期病変の免疫組織学的検討-モノクロナル抗体を用いた炎症性細胞の定性並びに定量的解析-。" リウマチ.

    • Related Report
      1989 Annual Research Report
  • [Publications] Sawai T: "insitu hybridization of Stromelysin mRNA in the synovial biopsies from Rheumatoid Arthritis." Am.J.Pathol.

    • Related Report
      1989 Annual Research Report
  • [Publications] 村上一宏: "MCTD患者血清中抗平滑筋作用因子の解析" 厚生省特定疾患混合性結合織病調査研究班平成元年度研究報告書.

    • Related Report
      1989 Annual Research Report
  • [Publications] 沢井高志他: 診断と治療. 76. 295-300 (1988)

    • Related Report
      1988 Annual Research Report
  • [Publications] 沢井高志他: リウマチ.

    • Related Report
      1988 Annual Research Report
  • [Publications] 沢井高志他: リウマチ.

    • Related Report
      1988 Annual Research Report
  • [Publications] 沢井高志他: "リウマチ学研究" 同文書院,

    • Related Report
      1988 Annual Research Report
  • [Publications] 村上一宏他: "臨床VisnalMook〓慢性関節リウマチ〓" 全日本病院出版会,

    • Related Report
      1988 Annual Research Report

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Published: 1988-04-01   Modified: 2016-04-21  

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