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Antitumor activity of monocyte-macrophages and its potentiation in cancer patients

Research Project

Project/Area Number 63570293
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field 内科学一般
Research InstitutionTokushima University School of Medicine

Principal Investigator

SONE Saburo  Tokushima Univ. Sch. of Med., Lecturer, 医学部・附属病院, 講師 (40145024)

Co-Investigator(Kenkyū-buntansha) OKUBO Akio  Tokushima University School of Medicine Assistant internist, 医学部・附属病院, 助手 (90203728)
Project Period (FY) 1988 – 1989
Project Status Completed (Fiscal Year 1989)
Budget Amount *help
¥2,000,000 (Direct Cost: ¥2,000,000)
Fiscal Year 1989: ¥900,000 (Direct Cost: ¥900,000)
Fiscal Year 1988: ¥1,100,000 (Direct Cost: ¥1,100,000)
KeywordsMonocyte / Alveolar macrophage / Monokine / Interleukin 1 / Lung cancer / tumor necrosis factor / モノカイン / マクロファージ モノカイン / インターロイキン1 / 腫瘍壊死因子 / 肺癌 / リポソーム / 細胞障害作用
Research Abstract

The present studies were performed to elucidate the mechaniss by which human activated mosocyte-macrophages kill tumor cells. Membrane-associated interieukin 1alpha (IL-1alpha ) induced by synergistic actions of IFN-gamma and synthetic adjutant, was found to be a mediator of human monodyte-mediated tumor cell killing. Human alveolar macrophages and monocyte-derived macrophages had abilities to produce cell-associated IL-1alpa activity and tamior necrosis factor (TNF) more than blood sonocytes. We also found that human monocyte-mediated cytotoxicity against human selanona (A375) cells was mediated in part by a new tumor cytotoxic factor (KW, 30,000; PI 6), differing from IL-1 and TMF-alpha. Abilities of blood monocytes of lung cancer patients to express antitamor activity and to produce IL-1 were analyzed. The presence of lung cancer significantly increased the number of barvested blood monocytes. Spontaneous tumoricidel activity of these monocutes was slightly high as compared to those of healthy donors. Nevertheless, there was no difference in production of IL-1 between two groups. Blood monocutes of lung cancer patients were less cytotaxic than healthy donors subsequent to incubation with synthetic immunoadjuvant, but were as tomoricidal as those from healthy donors when activated with liposome-entrapped immunoadjuvant. Human II-2-activated killer cells were cytotoxic to alveolar macrophages and to macrophates matured by GM-CSF from blood sonocytes. These observations suggest that alveolar macrophages may play a critical role in situ regulation of pulmonary inflammatory and immune reactions through production of cell- associated IL-1alpha and INF.

Report

(3 results)
  • 1989 Annual Research Report   Final Research Report Summary
  • 1988 Annual Research Report
  • Research Products

    (22 results)

All Other

All Publications (22 results)

  • [Publications] Okubo, A. et al.: "Membrane-associated interleukin 1 as a mediator of tumor cell killing by human blood monocytes fixed with paraformaldehyde" Cancer Research. 49. 265-270 (1989)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Kimura, S., et al.: "Antitumor potential of pleural cavity macrophages in lung cancer patients without malignant effusion" British Journal of Cancer 59:535-539, 1989.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Singh, S.M., et al.: "Up-regulation by granulocyte-macrophage colony stimulating factor of induction of lymphokine-activated killer (LAK) cells by human blood monocytes" International Journal of Cancer 44:662-669, 1989.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Inamura, N., et al.: "Tumor cytotoxicity of human monocyte membrane-IL-1 induced by synergistic actions of interferon r and synthetic acyltripeptide, FK-562" Cancer Immunology Immunotherapy 28:164-170, 1989.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Sone, S., et al.: "Killing of alveolar macrophages and of monocytes that have responded to granulocyte-macrophage colony stimulating factor by human lymphokine-activated killer cells" Japanese Journal of Cancer Research, 80:662-669, 1989.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Sone, S., et al.: "Normal human alveolar macrohages have more ability than blood monocytes to produce cell-associated interleukin 1" American Journal of Respiratory Cell and Molecular Biology, 1:507-515, 1989.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Sone, S., et al.: "Synergism of synthetic acyltripeptide and its analogs with recombinant interferon r for activation of antitumor properties of human blood monocytes" Cancer Immunology Immunotherapy 27:33-37, 1988.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Sone, S., et al.: "Activation of human monocyte-macrophages to the tumoricidal state by liposomal macropahge activators" Gann Monograph on Cancer Research 34:121-130, 1988.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Okubo,A.: "Membrane-bound interleukin 1α as a mediator of tumor cell killing by human blood monocytes fixed with paraformaldehyde" Cancer Research. 49. 265-270 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] Inamura,N.: "Tumor cytotoxicity of human monocyte membrane-bound IL-1α induced by synergistic actions of interferon γ and synthetic acyltripeptide,FK-565" Cancer Immunology Immunotherapy. 28. 164-170 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] Kimura,S.: "Antitumor potential of pleural cavity macrophages in lung cancer patients without malignant effusion" British Journal of Cancer. 59. 535-539 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] Singh,S.M.: "Up-regulation by granulocyte-macrophage colony stimulating factor(GM-CSF)of induction of lymphokine(IL-2)-activated killer(LAK)cells by human monocytes" International Journal of Cancer. 44. 170-176 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] Sone,S.: "Killing of alveolar macrophages and of monocytes that have responded to granulocyte-macrophage colony stimulating factor by human LAK cells" Japanese Journal of Cancer Research. 80. 662-669 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] Sone,S.: "Normal human alveolar macrophages have more ability than blood monocytes to produce cell-associated interleukin 1" American Journal of Respiratory Cell and Moleceular Biology. 1. 507-515 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] Sone,S.: "Liposomes in the Therapy of Infectious Diseases and Cancer" Alan R.Liss,Inc., 480 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] S.Sone,et al: The Journal of National Cancer Institute. 80. 425-431 (1988)

    • Related Report
      1988 Annual Research Report
  • [Publications] S.Sone,et al: Gann Monograph on Cancer Research. 34. 121-130 (1988)

    • Related Report
      1988 Annual Research Report
  • [Publications] S.Sone,et al: International Journal of Cancer. 42. 428-434 (1988)

    • Related Report
      1988 Annual Research Report
  • [Publications] S.Sone,et al: Cancer Immunology and Immumotherapy. 27. 33-37 (1988)

    • Related Report
      1988 Annual Research Report
  • [Publications] A.Okubo,et al.: Cancer Research. 49. 265-270 (1989)

    • Related Report
      1988 Annual Research Report
  • [Publications] N.Inamura: Cancer Immunology and Immunotherapy. 28. (1989)

    • Related Report
      1988 Annual Research Report
  • [Publications] S.Sone: "Liposomes in the Therapy of Infectious Diseases and Cancer" Alan R.Liss,Inc., 125-134 (1989)

    • Related Report
      1988 Annual Research Report

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Published: 1988-04-01   Modified: 2016-04-21  

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