プラズマ照射に対する細胞応答ダイナミクス－プラズマ医療の至適化に向けた基盤研究－

• Project Area: Plasma medical innovation
• Project/Area Number: 15H00900
• Research Category: Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area)
• Allocation Type: Single-year Grants
• Review Section: Science and Engineering
• Research Institution: Gifu Pharmaceutical University
• Principal Investigator: 足立 哲夫 岐阜薬科大学, 薬学部, 教授 (40137063)
• Project Period (FY): 2015-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥7,150,000 (Direct Cost: ¥5,500,000、Indirect Cost: ¥1,650,000); Fiscal Year 2016: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000); Fiscal Year 2015: ¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000)
• Keywords: プラズマ医療 / ストレス / シグナル伝達 / 活性酸素 / プラズマ / アポトーシス / 金属

Outline of Annual Research Achievements

１．近年、プラズマ照射の医療への応用が急速に進み、有為な効果が示されているものの、その効果を証明するメカニズムについては未解明な部分が多い。プラズマ照射法として、予めプラズマを照射して調製した培地plasma-activated medium（PAM）を負荷する間接法の方が「より安心・安全なプラズマ医療」に繋がると判断した。本申請課題では、PAM負荷に対する細胞応答ダイナミクスの解明を目的とし、今年度は以下に示す結果を得た。
２．肺胞基底上皮がん細胞A549細胞にPAMを負荷した場合、細胞内でのOHラジカルの発生、2価鉄レベルの上昇が認められた。また、DNA分断（TUNEL法）とその修復酵素であるPARP-1の活性化、それに誘引される反応として、NAD+の枯渇、ATPの枯渇、カルシウムイオンの細胞内流入も観察された。細胞内のフェリチンタンパクレベルの低下が認められたが、catalase、BP（鉄キレーター）、DMTU（OHラジカル消去剤）の他にMG132（プロテアソーム阻害剤）やleupeptin（リソソームプロテアーゼ阻害剤）によっても抑制された。以上の結果より、PAM負荷により細胞内で発生したOHラジカルによりフェリチンが破壊されて貯蔵されていた鉄イオンが遊離し、さらにOHラジカルが増加する連鎖反応でDNA分断やエネルギー枯渇が増幅され細胞死が誘導される可能性が示唆された。
３．緩和な条件で調製したmild PAMを線維芽細胞に負荷した場合のストレス抵抗性獲得メカニズムについて検討し、HO-1発現の亢進、Nrf2の核移行やそれのHO-1プロモータ上のAREへの結合を検出した。以上より、Keap1-Nrf2系の活性化による抗酸化酵素HO-1の発現亢進が酸化ストレス抵抗能の獲得に繋がっていることを明らかにした。

Research Progress Status

28年度が最終年度であるため、記入しない。

Strategy for Future Research Activity

28年度が最終年度であるため、記入しない。

Research Products

• [Journal Article] Introduction to serial reviews: Biomedical application of non-thermal atmospheric pressure plasma and its usefulness (2017)
  • Author(s): Adachi T
  • Journal Title: Journal of Clinical Biochemistry and Nutrition Volume: 60 Issue: 1 Pages: 1-2
  • DOI: 10.3164/jcbn.16-78
  • NAID: 130005188905
  • ISSN: 0912-0009, 1880-5086
  • Peer Reviewed
• [Journal Article] Differences in intracellular mobile zinc levels affect susceptibility to plasma-activated medium-induced cytotoxicity (2017)
  • Author(s): Hara H, Sueyoshi S, Taniguchi M, Kamiya T, Adachi T
  • Journal Title: Free Rad. Res. Volume: - Issue: 3 Pages: 1-10
  • DOI: 10.1080/10715762.2017.1309527
  • Peer Reviewed
• [Journal Article] Future perspective of strategic non-thermal plasma therapy for cancer treatment (2017)
  • Author(s): Kajiyama H, Utsumi F, Nakamura K, Tanaka H, Toyokuni S, Hori M, Kikkawa F
  • Journal Title: Journal of Clinical Biochemistry and Nutrition Volume: 60 Issue: 1 Pages: 33-38
  • DOI: 10.3164/jcbn.16-65
  • NAID: 130005188914
  • ISSN: 0912-0009, 1880-5086
  • Peer Reviewed
• [Journal Article] Medical applications of non-thermal atmospheric pressure plasma (2017)
  • Author(s): Tanaka H, Hori M
  • Journal Title: Journal of Clinical Biochemistry and Nutrition Volume: 60 Issue: 1 Pages: 29-32
  • DOI: 10.3164/jcbn.16-67
  • NAID: 130005188915
  • ISSN: 0912-0009, 1880-5086
  • Peer Reviewed
• [Journal Article] Intraperitoneal Administration of Plasma-Activated Medium: Proposal of a Novel Treatment Option for Peritoneal Metastasis From Gastric Cancer (2017)
  • Author(s): Takeda S, Yamada S, Hattori N, Nakamura K, Tanaka H, Kajiyama H, Kanda M, Kobayashi D, Tanaka C, Fujii T, Fujiwara M, Mizuno M, Hori M, Kodera Y
  • Journal Title: Ann. Surg. Oncol. Volume: 24 Pages: 1188-1194
  • Peer Reviewed
• [Journal Article] Iron stimulates plasma-activated medium-induced A549 cell injury (2016)
  • Author(s): Adachi T, Nonomura S, Horiba M, Hirayama T, Kamiya T, Nagasawa H, HaraH
  • Journal Title: Sci Reports Volume: 6 Issue: 1 Pages: 20928-20928
  • DOI: 10.1038/srep20928
  • Peer Reviewed / Open Access
• [Journal Article] Histone deacetylase inhibitors stimulate the susceptibility of A549 cells to a plasma-activated medium treatment (2016)
  • Author(s): Adachi T, Kano A, Nonomura S, Kamiya T, Hara H
  • Journal Title: Arch. Biochem. Biophys. Volume: 606 Pages: 120-127
  • DOI: 10.1016/j.abb.2016.07.019
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Low-temperature plasma in biology and medicine (2016)
  • Author(s): Hori M, Choi EH, Toyokuni S
  • Journal Title: Arch. Biochem. Biophys. Volume: 605 Pages: 1-2
  • DOI: 10.1016/j.abb.2016.06.014
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] The origin and future of oxidative stress pathology: From the recognition of carcinogenesis as an iron addiction with ferroptosis-resistance to non-thermal plasma therapy. (2016)
  • Author(s): Toyokuni S.
  • Journal Title: Pathol Int Volume: in press Issue: 5 Pages: 245-259
  • DOI: 10.1111/pin.12396
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Non-thermal plasma prevents progression of endometriosis in mice (2016)
  • Author(s): Ishida C, Mori M, Nakamura K, Tanaka H, Mizuno M, Hori M, Iwase A, Kikkawa F, Toyokuni S
  • Journal Title: Free Radic. Res. Volume: 50 Pages: 1131-1139
  • Peer Reviewed
• [Journal Article] Biphasic effects of l-ascorbate on the tumoricidal activity of non-thermal plasma against malignant mesothelioma cells (2016)
  • Author(s): Shi L, Wang Y, Ito F, Okazaki Y, Tanaka H, Mizuno M, Hori M, Richardson DR, Toyokuni S
  • Journal Title: Arch. Biochem. Biophys. Volume: 605 Pages: 109-116
  • DOI: 10.1016/j.abb.2016.05.016
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Variable susceptibility of ovarian cancer cells to non-thermal plasma-activated medium. (2016)
  • Author(s): Utsumi F, Kajiyama H, Nakamura K, Tanaka H, Mizuno M, Toyokuni S, Hori M, Kikkawa F.
  • Journal Title: Oncol Rep. Volume: in press Issue: 6 Pages: 3169-3177
  • DOI: 10.3892/or.2016.4726
  • Peer Reviewed / Open Access
• [Journal Article] Cell survival of glioblastoma grown in medium containing hydrogen peroxide and/or nitrite, or in plasma-activated medium (2016)
  • Author(s): Kurake N, Tanaka H, Ishikawa K, Kondo T, Sekine M, Nakamura K, Kajiyama Hi, Kikkaw F, Mizun M, Hori M
  • Journal Title: Arch Biochem Biophys Volume: 未定 Pages: 102-108
  • DOI: 10.1016/j.abb.2016.01.011
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Plasma-activated medium-induced A549 cell injury by a spiral apoptotic cascade involving the mitochondrial-nuclear network and plasma membrane (2015)
  • Author(s): T. Adachi, H. Tanaka, H. Hara, S. Kondo, M. Hori
  • Journal Title: Free Radical Biology & Medicine Volume: 79 Pages: 28-44
  • DOI: 10.1016/j.freeradbiomed.2014.11.014
  • Peer Reviewed / Open Access
• [Journal Article] Plasma-activated medium-induced intracellular zinc liberation causes death of SH-SY5Y cells (2015)
  • Author(s): Hara H, Taniguchi M, Kobayashi M, Kamiya T, Adachi T
  • Journal Title: Arch Biochem Biophys Volume: 584 Pages: 51-60
  • DOI: 10.1016/j.abb.2015.08.014
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Plasma-Activated Solution: Mechanism of Action, Clinical Application, and Industrialization (2015)
  • Author(s): Tanaka H, Mizuno M, Toyokuni S, Maruyama S, Kodera Y, Kikkawa F, Hori M
  • Journal Title: Fukuoka Igaku Zasshi Volume: 106 Pages: 71-76
  • Peer Reviewed
• [Journal Article] Effectiveness of plasma treatment on pancreatic cancer cells (2015)
  • Author(s): N. Hattori, S. Yamada, K. Torii, S. Takeda, K. Nakamura, H. Tanaka, H. Kajiyama, M. Kanda, T. Fujii, G. Nakayama, H. Sugimoto, M. Koike, S. Nomoto, M. Fujiwara, M. Mizuno, M. Hori, Y. Kodera
  • Journal Title: International journal of oncology Volume: 47 Issue: 5 Pages: 1655-1662
  • DOI: 10.3892/ijo.2015.3149
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Plasma-activated medium suppresses choroidal neovascularization in mice: a new therapeutic concept for age-related macular degeneration (2015)
  • Author(s): F. Ye, H. Kaneko, Y. Nagasaka, R. Ijima, K. Nakamura, M. Nagaya, K. Takayama, H. Kajiyama, T. Senga, H. Tanaka, M. Mizuno, F. Kikkawa, M. Hori, and H. Terasaki
  • Journal Title: Sci Rep Volume: 5 Issue: 1 Pages: 7705-7705
  • DOI: 10.1038/srep07705
  • Peer Reviewed / Open Access
• [Presentation] 大気圧プラズマ負荷に対するがん細胞応答機構 (2017)
  • Author(s): 原 宏和、足立哲夫
  • Organizer: 日本薬学会第137年会
  • Place of Presentation: 仙台国際センター（宮城県、仙台市）
  • Year and Date: 2017-03-25
  • Invited
• [Presentation] Intracellular Free Zinc Mediates Cellular Responses to PAM (2017)
  • Author(s): Hara H, Adachi T
  • Organizer: The 1st International conference on plasma medical science innovation (ICPMSI2017)
  • Place of Presentation: 名古屋大学（愛知県、名古屋市）
  • Year and Date: 2017-02-27
  • Int'l Joint Research
• [Presentation] State of the arts in plasma medical science (2017)
  • Author(s): Hori M, Tanaka H, Ishikawa K, Toyokuni S, Kikkawa F, et al.
  • Organizer: The 1st International conference on plasma medical science innovation (ICPMSI2017)
  • Place of Presentation: 名古屋大学（愛知県、名古屋市）
  • Year and Date: 2017-02-27
  • Int'l Joint Research
• [Presentation] The effect of aqueous therapy targeting for occult peritoneal metastasis of ovarian cancer (2017)
  • Author(s): Kajiyama H, Tanaka H, Toyokuni S, Hori M, Kikkawa F, et al.
  • Organizer: The 1st International conference on plasma medical science innovation (ICPMSI2017)
  • Place of Presentation: 名古屋大学（愛知県、名古屋市）
  • Year and Date: 2017-02-27
  • Int'l Joint Research
• [Presentation] Behavior of OH radical in gas-liquid interface region on ac excited non-equilibrium atmospheric pressure plasma jet irradiation to liquid (2017)
  • Author(s): Kuramashi R, Ishikawa K, Tanaka H, Hori M, et al.
  • Organizer: The 1st International conference on plasma medical science innovation (ICPMSI2017)
  • Place of Presentation: 名古屋大学（愛知県、名古屋市）
  • Year and Date: 2017-02-27
  • Int'l Joint Research
• [Presentation] Anti-tumor effects of plasma-activated medium which contains fullerenol (2017)
  • Author(s): Kanno D, Tanaka H, Ishikawa K, Hori M, et al.
  • Organizer: The 1st International conference on plasma medical science innovation (ICPMSI2017)
  • Place of Presentation: 名古屋大学（愛知県、名古屋市）
  • Year and Date: 2017-02-27
  • Int'l Joint Research
• [Presentation] Antitumor effects of laser-generated plasma activated medium (LPAM) with and without catalase (2017)
  • Author(s): Kurokawa Y, Ishikawa K, Tanaka H, Hori M, et al.
  • Organizer: The 1st International conference on plasma medical science innovation (ICPMSI2017)
  • Place of Presentation: 名古屋大学（愛知県、名古屋市）
  • Year and Date: 2017-02-27
  • Int'l Joint Research
• [Presentation] Elucidation of the effects of plasma-activated medium on supported lipid bilayers by employing high-speed atomic force microscopy (2017)
  • Author(s): Tonami T, Ishikawa K, Tanaka H, Hori M, et al.
  • Organizer: The 1st International conference on plasma medical science innovation (ICPMSI2017)
  • Place of Presentation: 名古屋大学（愛知県、名古屋市）
  • Year and Date: 2017-02-27
  • Int'l Joint Research
• [Presentation] Intracellular changes of reactive oxygen and nitrogen species in HeLa cells induced by plasma-activated medium (2017)
  • Author(s): Furuta R, Ishikawa K, Tanaka H, Hori M, et al.
  • Organizer: The 1st International conference on plasma medical science innovation (ICPMSI2017)
  • Place of Presentation: 名古屋大学（愛知県、名古屋市）
  • Year and Date: 2017-02-27
  • Int'l Joint Research
• [Presentation] Antitumor effect of culture medium irradiated with non-equilibrium atmospheric pressure plasmas under purge of ambient by N2 and O2 mixtures (2017)
  • Author(s): Hosoi Y, Ishikawa K, Tanaka H, Hori M, et al.
  • Organizer: The 1st International conference on plasma medical science innovation (ICPMSI2017)
  • Place of Presentation: 名古屋大学（愛知県、名古屋市）
  • Year and Date: 2017-02-27
  • Int'l Joint Research
• [Presentation] Synthesis and crystallization of oxalates in glucose-containing liquid irradiated with non-equilibrium atmospheric pressure plasmas (2017)
  • Author(s): Kurake N, Tanaka H, Ishikawa K, Hori M, et al.
  • Organizer: The 1st International conference on plasma medical science innovation (ICPMSI2017)
  • Place of Presentation: 名古屋大学（愛知県、名古屋市）
  • Year and Date: 2017-02-27
  • Int'l Joint Research
• [Presentation] A mechanism on generations of H2O2 and NO2- in plasma activated medium (2017)
  • Author(s): Kurake N, Tanaka H, Ishikawa K, Hori M, et al.
  • Organizer: The 1st International conference on plasma medical science innovation (ICPMSI2017)
  • Place of Presentation: 名古屋大学（愛知県、名古屋市）
  • Year and Date: 2017-02-27
  • Int'l Joint Research
• [Presentation] Enhancement of cancer cell killing effect of hydrogen peroxide by nitrite existence in plasma activated medium (2017)
  • Author(s): Kurake N, Tanaka H, Ishikawa K, Hori M, et al.
  • Organizer: The 1st International conference on plasma medical science innovation (ICPMSI2017)
  • Place of Presentation: 名古屋大学（愛知県、名古屋市）
  • Year and Date: 2017-02-27
  • Int'l Joint Research
• [Presentation] Time evolution of reactive oxygen nitrogen species in plasma-activated liquids (2017)
  • Author(s): Brubaker TR, Ishikawa K, Tanaka H, Hori M, et al.
  • Organizer: The 1st International conference on plasma medical science innovation (ICPMSI2017)
  • Place of Presentation: 名古屋大学（愛知県、名古屋市）
  • Year and Date: 2017-02-27
  • Int'l Joint Research
• [Presentation] Effects of dielectric walls on atmospheric-pressure plasma discharges (2017)
  • Author(s): Katsuno K, Ishikawa K, Tanaka H, Hori M, et al.
  • Organizer: The 1st International conference on plasma medical science innovation (ICPMSI2017)
  • Place of Presentation: 名古屋大学（愛知県、名古屋市）
  • Year and Date: 2017-02-27
  • Int'l Joint Research
• [Presentation] Dynamic observations of morphology of HeLa cell membranes in plasma activated medium by high-speed atomic force microscopy (2017)
  • Author(s): Yamaoka S, Ishikawa K, Tanaka H, Hori M, et al.
  • Organizer: The 1st International conference on plasma medical science innovation (ICPMSI2017)
  • Place of Presentation: 名古屋大学（愛知県、名古屋市）
  • Year and Date: 2017-02-27
  • Int'l Joint Research
• [Presentation] Measurement of HOO in oxygen-radical-treated water using electron spin resonance (2017)
  • Author(s): Kobayashi T, Ishikawa K, Hori M, et al.
  • Organizer: The 1st International conference on plasma medical science innovation (ICPMSI2017)
  • Place of Presentation: 名古屋大学（愛知県、名古屋市）
  • Year and Date: 2017-02-27
  • Int'l Joint Research
• [Presentation] Efficiency of intraperitoneal therapy with plasma-activated medium (PAM) targeting micrometastasis in gastric cancer in nude mice (2017)
  • Author(s): Nakanishi H, Tanaka H, Ishikawa K, Hori M, Ikehara Y, et al.
  • Organizer: The 1st International conference on plasma medical science innovation (ICPMSI2017)
  • Place of Presentation: 名古屋大学（愛知県、名古屋市）
  • Year and Date: 2017-02-27
  • Int'l Joint Research
• [Presentation] Plasma activated medium stimulation may affect low serum cultured ASCs cytokine secretion (2017)
  • Author(s): Kamimura Y, Tanaka H, Hori M, Maruyama S, et al.
  • Organizer: The 1st International conference on plasma medical science innovation (ICPMSI2017)
  • Place of Presentation: 名古屋大学（愛知県、名古屋市）
  • Year and Date: 2017-02-27
  • Int'l Joint Research
• [Presentation] Anti-cancer effect on cancer-initiating cells treated with plasma-activated medium (PAM) in cancer cells (2017)
  • Author(s): Ikeda J, Tanaka H, Ishikawa K, Hori M, et al.
  • Organizer: The 1st International conference on plasma medical science innovation (ICPMSI2017)
  • Place of Presentation: 名古屋大学（愛知県、名古屋市）
  • Year and Date: 2017-02-27
  • Int'l Joint Research
• [Presentation] Effect of plasma-treatment on the in vitro growth of Trypanosoma Brucei (2017)
  • Author(s): Yokoyama N, Ishikawa K, Hori M, Ikehara Y, et al.
  • Organizer: The 1st International conference on plasma medical science innovation (ICPMSI2017)
  • Place of Presentation: 名古屋大学（愛知県、名古屋市）
  • Year and Date: 2017-02-27
  • Int'l Joint Research
• [Presentation] プラズマ照射に対する細胞応答の分子機構 (2016)
  • Author(s): 堀場美の里，加納綾女，神谷哲朗，原 宏和，足立哲夫
  • Organizer: 新学術領域「プラズマ医療科学の創成」全体会議
  • Place of Presentation: 大宰府館（福岡県、大宰府市）
  • Year and Date: 2016-12-23
• [Presentation] プラズマ照射培地負荷によるがん細胞死に対するヒストン脱アセチル化酵素阻害剤の増強効果 (2016)
  • Author(s): 加納綾女，野々村早帆，神谷哲朗，原 宏和，足立哲夫
  • Organizer: 日本病院薬剤師会東海ブロック・日本薬学会東海支部合同学術大会2016
  • Place of Presentation: 長良川国際会議場（岐阜県、岐阜市）
  • Year and Date: 2016-10-30
• [Presentation] ヒト表皮角化細胞HaCaTにおけるプラズマ照射によるIL-8発現誘導機構 (2016)
  • Author(s): 堀田英美里，原 宏和，神谷哲朗，足立哲夫
  • Organizer: 日本病院薬剤師会東海ブロック・日本薬学会東海支部合同学術大会2016
  • Place of Presentation: 長良川国際会議場（岐阜県、岐阜市）
  • Year and Date: 2016-10-30
• [Presentation] Intracellular Free Zinc Plays an Important Role in Plasma-Activated Medium-Induced Cell Death (2016)
  • Author(s): Hara H, Taniguchi M, Kobayasi K, Sueyoshi S, Kamiya T, Adachi T
  • Organizer: 6th International Conference on Plasma Medicine (ICPM-6)
  • Place of Presentation: Bratislava (Slovakia)
  • Year and Date: 2016-09-04
  • Int'l Joint Research
• [Presentation] Insight into chemical mechanism in plasma medicine from viewpoints of oxidative stress (2016)
  • Author(s): Toyokuni S
  • Organizer: 6th International Conference on Plasma Medicine (ICPM-6)
  • Place of Presentation: Bratislava (Slovakia)
  • Year and Date: 2016-09-04
  • Int'l Joint Research / Invited
• [Presentation] Plasma-activated medium and cancer (2016)
  • Author(s): Tanaka H, Mizuno M, Ishikawa K, Toyokuni S, Hori M, et al.
  • Organizer: 6th International Conference on Plasma Medicine (ICPM-6)
  • Place of Presentation: Bratislava (Slovakia)
  • Year and Date: 2016-09-04
  • Int'l Joint Research / Invited
• [Presentation] Metabolic profiles on glioblastoma (U251SP) modified in plasma-activated medium (PAM) cultivation (2016)
  • Author(s): Ishikawa K, Tanaka H, Hori M, et al.
  • Organizer: 6th International Conference on Plasma Medicine (ICPM-6)
  • Place of Presentation: Bratislava (Slovakia)
  • Year and Date: 2016-09-04
  • Int'l Joint Research
• [Presentation] Dynamic behavior of HeLa cells in plasma-activated medium (2016)
  • Author(s): Furuta R, Ishikawa, Tanaka H, Hori M, et al.
  • Organizer: 6th International Conference on Plasma Medicine (ICPM-6)
  • Place of Presentation: Bratislava (Slovakia)
  • Year and Date: 2016-09-04
  • Int'l Joint Research
• [Presentation] Selective killing of human breast cancer cells by plasma-activated medium (2016)
  • Author(s): Hashizume H, Tanaka H, Ishikawa K, Hori M, et al.
  • Organizer: 6th International Conference on Plasma Medicine (ICPM-6)
  • Place of Presentation: Bratislava (Slovakia)
  • Year and Date: 2016-09-04
  • Int'l Joint Research
• [Presentation] Plasma activated medium change ASCs cytokine profile (2016)
  • Author(s): Kamimura Y, Tanaka H, Hori M, Maruyama s, et al.
  • Organizer: 6th International Conference on Plasma Medicine (ICPM-6)
  • Place of Presentation: Bratislava (Slovakia)
  • Year and Date: 2016-09-04
  • Int'l Joint Research
• [Presentation] Plasma-activated medium inhibits metastatic tendency of ovarian cancer cells in vitro (2016)
  • Author(s): Peng Y, Tanaka H, Toyokuni S, Hori M, Kikkawa F, et al.
  • Organizer: 6th International Conference on Plasma Medicine (ICPM-6)
  • Place of Presentation: Bratislava (Slovakia)
  • Year and Date: 2016-09-04
  • Int'l Joint Research
• [Presentation] Inactivation factors of microorganisms in solution treated with neutral oxygen radical source (2016)
  • Author(s): Ito M, Ishikawa K, Hori M, et al.
  • Organizer: 6th International Conference on Plasma Medicine (ICPM-6)
  • Place of Presentation: Bratislava (Slovakia)
  • Year and Date: 2016-09-04
  • Int'l Joint Research
• [Presentation] Generation of active species in laser-induced-plasma activated medium (2016)
  • Author(s): Kurokawa Y, Ishikawa K, Tanaka H, Hori M, et al.
  • Organizer: 6th International Conference on Plasma Medicine (ICPM-6)
  • Place of Presentation: Bratislava (Slovakia)
  • Year and Date: 2016-09-04
  • Int'l Joint Research
• [Presentation] Plasma-activated liquid therapy suppresses intraperitoneal metastasis of chemoresistant ovarian cancer (2016)
  • Author(s): Kajiyama H, Tanaka H, Hori M, Kikkawa F, et al.
  • Organizer: 6th International Conference on Plasma Medicine (ICPM-6)
  • Place of Presentation: Bratislava (Slovakia)
  • Year and Date: 2016-09-04
  • Int'l Joint Research
• [Presentation] Effect of plasma-activated medium (PAM) on cancer-initiating cells for cancer therapy (2016)
  • Author(s): Ikeda J, Tanaka H, Ishikawa K, Hori M, Ikehara Y, et al.
  • Organizer: 6th International Conference on Plasma Medicine (ICPM-6)
  • Place of Presentation: Bratislava (Slovakia)
  • Year and Date: 2016-09-04
  • Int'l Joint Research
• [Presentation] Health effects of repeated intraperitoneal injections of plasma-activated medium in mice (2016)
  • Author(s): Tanaka A, Tanaka H, Hori M, et al.
  • Organizer: 6th International Conference on Plasma Medicine (ICPM-6)
  • Place of Presentation: Bratislava (Slovakia)
  • Year and Date: 2016-09-04
  • Int'l Joint Research
• [Presentation] Effect of intraperitoneal therapy with plasma-activated medium targeting peritoneal micrometastasis as revealed by GFP-tagged human gastric cancer cell lines in nude mice (2016)
  • Author(s): Nakanishi H, Tanaka H, Hori M, Ikehara Y, et al.
  • Organizer: 6th International Conference on Plasma Medicine (ICPM-6)
  • Place of Presentation: Bratislava (Slovakia)
  • Year and Date: 2016-09-04
  • Int'l Joint Research
• [Presentation] Plasma-activated medium (PAM) inhibits peritoneal metastasis in ovarian cancer mouse model (2016)
  • Author(s): Nakamura K, Tanaka H, Toyokuni S, Hori M, Kikkawa F, et al.
  • Organizer: 6th International Conference on Plasma Medicine (ICPM-6)
  • Place of Presentation: Bratislava (Slovakia)
  • Year and Date: 2016-09-04
  • Int'l Joint Research
• [Presentation] Particulates generation in the plasma activated medium (PAM) (2016)
  • Author(s): Kurake N, Tanaka H, Ishikawa K, Hori M, et al.
  • Organizer: 6th International Conference on Plasma Medicine (ICPM-6)
  • Place of Presentation: Bratislava (Slovakia)
  • Year and Date: 2016-09-04
  • Int'l Joint Research
• [Presentation] ヒストン脱アセチル化酵素阻害剤はがん細胞のプラズマ照射培地に対する感受性を増強する (2016)
  • Author(s): 足立哲夫，加納綾女，野々村早帆，神谷哲朗，原 宏和
  • Organizer: 第69回日本酸化ストレス学会学術集会
  • Place of Presentation: 仙台国際センター（宮城県、仙台市）
  • Year and Date: 2016-08-30
• [Presentation] プラズマ活性化溶液（PAM）培養した脳腫瘍細胞（U251SP）の代謝プロファイル (2016)
  • Author(s): 石川健治、田中宏昌、堀勝、他
  • Organizer: 第69回日本酸化ストレス学会学術集会
  • Place of Presentation: 仙台国際センター（宮城県、仙台市
  • Year and Date: 2016-08-30
• [Presentation] プラズマ活性溶液の安全性評価について (2016)
  • Author(s): 古田恭寛、田中宏昌、豊國伸哉、他
  • Organizer: 第69回日本酸化ストレス学会学術集会
  • Place of Presentation: 仙台国際センター（宮城県、仙台市
  • Year and Date: 2016-08-30
• [Presentation] プラズマ照射培地より惹起されるA549細胞増殖抑制における細胞内亜鉛の関与 (2016)
  • Author(s): 小林真理，原 宏和，末吉紗也子，神谷哲朗，足立哲夫
  • Organizer: 第62回日本薬学会東海支部大会
  • Place of Presentation: 愛知学院大学（愛知県、名古屋市）
  • Year and Date: 2016-07-02
• [Presentation] ホルボールエステル誘導性の炎症反応に対するプラズマ照射培地の影響 (2016)
  • Author(s): 福留早貴，國吉真子，神谷哲朗，原宏和，足立哲夫
  • Organizer: 第62回日本薬学会東海支部大会
  • Place of Presentation: 愛知学院大学（愛知県、名古屋市）
  • Year and Date: 2016-07-02
• [Presentation] プラズマ照射培地による細胞傷害と細胞内亜鉛動態の関連性 (2016)
  • Author(s): 末吉紗也子, 原 宏和, 谷口美紘, 神谷哲朗, 足立哲夫
  • Organizer: 日本生化学会中部支部例会・シンポジウム
  • Place of Presentation: 三重大学（三重県、津市）
  • Year and Date: 2016-05-21
• [Presentation] プラズマ照射に対する細胞応答ダイナミクス (2016)
  • Author(s): 足立哲夫，野々村早帆，堀場美の里，神谷哲朗，原 宏和
  • Organizer: 名古屋大学「プラズマ科学プラットフォーム」開所記念講演会
  • Place of Presentation: 名古屋大学（愛知県、名古屋市）
  • Year and Date: 2016-04-26
• [Presentation] プラズマ照射に対する細胞応答ダイナミクス (2016)
  • Author(s): 足立哲夫，野々村早帆，堀場美の里，神谷哲朗，原 宏和
  • Organizer: 名古屋大学「プラズマ科学プラットフォーム」開所記念講演会
  • Place of Presentation: 名古屋
  • Year and Date: 2016-04-26
• [Presentation] プラズマ照射培地負荷によるヒト線維芽細胞での酸化ストレス抵抗性の獲得 (2016)
  • Author(s): 堀場美の里，神谷哲朗，原 宏和，足立哲夫
  • Organizer: 日本薬学会第136年会
  • Place of Presentation: 横浜
  • Year and Date: 2016-03-26
• [Presentation] プラズマ照射に対する細胞応答の分子機構 (2016)
  • Author(s): 原 宏和，神谷哲朗，足立哲夫
  • Organizer: 第４回新学術領域「プラズマ医療科学の創成」全体会議
  • Place of Presentation: 名古屋
  • Year and Date: 2016-03-06
• [Presentation] プラズマ照射培地によるSH-SY5Y細胞傷害の分子機構 －正常細胞との比較－ (2016)
  • Author(s): 原 宏和，谷口美紘，小林真理，神谷哲朗，足立哲夫
  • Organizer: 日本酸化ストレス学会東海支部 第4回学術集会
  • Place of Presentation: 鈴鹿
  • Year and Date: 2016-02-06
• [Presentation] プラズマ照射培地負荷に対する細胞応答の分子機構 (2016)
  • Author(s): 足立哲夫
  • Organizer: 仙台"プラズマフォーラム"
  • Place of Presentation: 仙台
  • Year and Date: 2016-01-22
• [Presentation] プラズマ照射により惹起される細胞傷害における亜鉛の役割 (2015)
  • Author(s): 原 宏和，谷口美紘，小林真理，神谷哲朗，足立哲夫
  • Organizer: メタルバイオサイエンス研究会2015
  • Place of Presentation: 名古屋
  • Year and Date: 2015-08-27
• [Presentation] PAMによる細胞傷害の分子機構 ―細胞内Zn2+の役割― (2015)
  • Author(s): 原 宏和，谷口美紘，小林真理，神谷哲朗，足立哲夫
  • Organizer: 新学術領域研究合同公開シンポジウム
  • Place of Presentation: 名古屋
  • Year and Date: 2015-08-05
• [Presentation] プラズマ照射培地による癌細胞アポトーシスへの鉄イオンの関与 (2015)
  • Author(s): 足立哲夫，野々村早帆，堀場美の里，平山 祐，神谷哲朗，永澤秀子，原 宏和
  • Organizer: 第68回日本酸化ストレス学会学術集会
  • Place of Presentation: 鹿児島
  • Year and Date: 2015-06-11
• [Presentation] プラズマ照射に対する細胞応答の分子機構－安全なプラズマ医療を目指した学術基盤 (2015)
  • Author(s): 足立哲夫
  • Organizer: 新学術領域研究「プラズマ医療科学の創成」キックオフミーティング
  • Place of Presentation: 福岡
  • Year and Date: 2015-05-23
• [Remarks] 岐阜薬科大学臨床薬剤学研究室ホームページ
  • URL: http://www.gifu-pu.ac.jp/lab/rinyaku/index.html