機能性核酸の細胞内動的構造解析とＤＮＡ変換酵素のスライディングの活写

• Project Area: Novel measurement techniques for visualizing 'live' protein molecules at work
• Project/Area Number: 17H05878
• Research Category: Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area)
• Allocation Type: Single-year Grants
• Review Section: Complex systems
• Research Institution: Kyoto University
• Principal Investigator: 永田 崇 京都大学, エネルギー理工学研究所, 准教授 (10415250)
• Project Period (FY): 2017-04-01 – 2019-03-31
• Project Status: Completed (Fiscal Year 2018)
• Budget Amount: ¥13,000,000 (Direct Cost: ¥10,000,000、Indirect Cost: ¥3,000,000); Fiscal Year 2018: ¥6,500,000 (Direct Cost: ¥5,000,000、Indirect Cost: ¥1,500,000); Fiscal Year 2017: ¥6,500,000 (Direct Cost: ¥5,000,000、Indirect Cost: ¥1,500,000)
• Keywords: APOBEC / in-cell NMR / AFM / スライディング / 核酸 / NMR / DNAオリガミ

Outline of Annual Research Achievements

A3G及びA3Fはレトロウィルスが合成するssDNA上のシトシンをウラシルに脱アミノ化することにより遺伝情報を破壊する。本研究では、A3G及びA3Fが一本鎖DNA中の標的シトシンを探索する際の分子機構の解明を目指して来た。これまでA3Gについて解析を進め成果を上げてきたが、今回はA3Fの解析を行い論文発表した[BBA, 2020]。まず、A3FのN214H変異体の活性が高いことを見出し [PCCP, 2018a]、これを使うことによりNMR解析が可能であることに思い至った。そして、これまで用いてきたuracil-DNA glycosylaseアッセイに加え、実時間NMR法も用いて脱アミノ化酵素活性を調べた。これにより、A3Fは濃度条件[A3F-CTD]≫[ssDNA]では長いssDNA中の標的シトシンを効率よく脱アミノ化するのに対し、[A3F-CTD]≪[ssDNA]条件では短いssDNA中の標的シトシンを効率よく脱アミノ化することが明らかとなった[BBA, 2020]。さらに本研究では、脱アミノ化速度とssDNA結合親和性を使って半定量解析することにより、A3Fのこの特徴的な活性について合理的に説明することにも成功した[BBA, 2020]。他方、機能性核酸の細胞内動的構造解析については、これまでにヒト培養細胞に導入した核酸のin-cell NMRに成功していた[PCCP, 2018b]。核酸のイミノ水素は塩基対を形成したときのみNMRシグナルとして観測される。したがって、イミノ水素と水の水素の交換速度を知ることにより、核酸構造の局所的な安定性を評価することができる。我々は、ヒト培養細胞に導入した核酸についてこの交換速度を測定するin-cell NMR法を開発し、核酸構造の安定性に関して議論することに成功し、第18回蛋白質科学会年会において口頭発表を行った。

Research Progress Status

平成30年度が最終年度であるため、記入しない。

Strategy for Future Research Activity

平成30年度が最終年度であるため、記入しない。

Research Products

• [Int'l Joint Research] Goethe University Frankfurt am Main(Germany)
• [Journal Article] Development and structural determination of an anti-PrPC aptamer that blocks pathological conformational conversion of prion protein (2020)
  • Author(s): Mashima Tsukasa、Lee Joon-Hwa、Kamatari Yuji O.、Hayashi Tomohiko、Nagata Takashi、Nishikawa Fumiko、Nishikawa Satoshi、Kinoshita Masahiro、Kuwata Kazuo、Katahira Masato
  • Journal Title: Scientific Reports Volume: 10 Issue: 1 Pages: 4934-4934
  • DOI: 10.1038/s41598-020-61966-4
  • NAID: 120006813315
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Recent progress of in-cell NMR of nucleic acids in living human cells (2020)
  • Author(s): Yamaoki, Y., Nagata, T., Sakamoto, T., and Katahira, M.
  • Journal Title: Biophysical Reviews Volume: - Issue: 2 Pages: 411-417
  • DOI: 10.1007/s12551-020-00664-x
  • Peer Reviewed / Open Access
• [Journal Article] RNA sequence and length contribute to RNA-induced conformational change of TLS/FUS (2020)
  • Author(s): Hamad Nesreen、Mashima Tsukasa、Yamaoki Yudai、Kondo Keiko、Yoneda Ryoma、Oyoshi Takanori、Kurokawa Riki、Nagata Takashi、Katahira Masato
  • Journal Title: Scientific Reports Volume: 10 Issue: 1 Pages: 2629-2629
  • DOI: 10.1038/s41598-020-59496-0
  • Peer Reviewed / Open Access
• [Journal Article] Suppression of Fast Proton Conduction by Dilution of a Hydronium Solvate Ionic Liquid: Localization of Ligand Exchange (2020)
  • Author(s): Kawata Kio、Kitada Atsushi、Tsuchida Naoki、Saimura Masayuki、Nagata Takashi、Katahira Masato、Fukami Kazuhiro、Murase Kuniaki
  • Journal Title: Journal of The Electrochemical Society Volume: 167 Issue: 4 Pages: 046508-046508
  • DOI: 10.1149/1945-7111/ab75fc
  • NAID: 120007006216
  • Peer Reviewed / Open Access
• [Journal Article] How Does the Recently Discovered Peptide MIP Exhibit Much Higher Binding Affinity than an Anticancer Protein p53 for an Oncoprotein MDM2 (2019)
  • Author(s): Yamada Tatsuya、Hayashi Tomohiko、Hikiri Simon、Kobayashi Naohiro、Yanagawa Hiroshi、Ikeguchi Mitsunori、Katahira Masato、Nagata Takashi、Kinoshita Masahiro
  • Journal Title: Journal of Chemical Information and Modeling Volume: 59 Issue: 8 Pages: 3533-3544
  • DOI: 10.1021/acs.jcim.9b00226
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] An insight into the dependence of the deamination rate of human APOBEC3F on the length of single-stranded DNA, which is affected by the concentrations of APOBEC3F and single-stranded DNA (2019)
  • Author(s): Wan Li、Kamba Keisuke、Nagata Takashi、Katahira Masato
  • Journal Title: Biochimica et Biophysica Acta (BBA) - General Subjects Volume: In Press Issue: 2 Pages: 129346-129346
  • DOI: 10.1016/j.bbagen.2019.04.011
  • Peer Reviewed / Open Access
• [Journal Article] Mechanism of protein-RNA recognition: analysis based on the statistical mechanics of hydration (2018)
  • Author(s): Hayashi Tomohiko, Matsuda Tomoaki, Nagata Takashi, Katahira Masato, Kinoshita Masahiro
  • Journal Title: Phys Chem Chem Phys Volume: 20 Issue: 14 Pages: 9167-9180
  • DOI: 10.1039/c8cp00155c
  • Peer Reviewed / Open Access
• [Journal Article] Characterization of the glutathione S-transferases that belong to the GSTFuA class in Ceriporiopsis subvermispora : Implications in intracellular detoxification and metabolism of wood-derived compounds (2018)
  • Author(s): Osman Wan Hasnidah Wan、Lin Meng-I、Kondo Keiko、Nagata Takashi、Katahira Masato
  • Journal Title: Int J Biol Macromol Volume: 113 Pages: 1158-1166
  • DOI: 10.1016/j.ijbiomac.2018.03.029
  • Peer Reviewed / Open Access
• [Journal Article] Characterisation of an aptamer against the Runt domain of AML1 (RUNX1) by NMR and mutational analyses (2018)
  • Author(s): Takada Kenta、Amano Ryo、Nomura Yusuke、Tanaka Yoichiro、Sugiyama Shigeru、Nagata Takashi、Katahira Masato、Nakamura Yoshikazu、Kozu Tomoko、Sakamoto Taiichi
  • Journal Title: FEBS Open Bio Volume: 8 Issue: 2 Pages: 264-270
  • DOI: 10.1002/2211-5463.12368
  • NAID: 120006457124
  • Peer Reviewed / Open Access
• [Journal Article] Plastic roles of phenylalanine and tyrosine residues of TLS/FUS in complex formation with the G-quadruplexes of telomeric DNA and TERRA (2018)
  • Author(s): Kondo Keiko、Mashima Tsukasa、Oyoshi Takanori、Yagi Ryota、Kurokawa Riki、Kobayashi Naohiro、Nagata Takashi、Katahira Masato
  • Journal Title: Scientific Reports Volume: 8 Issue: 1 Pages: 2864-2864
  • DOI: 10.1038/s41598-018-21142-1
  • Peer Reviewed / Open Access
• [Journal Article] High yield production of fungal manganese peroxidases by E. coli through soluble expression, and examination of the activities (2018)
  • Author(s): Lin Meng-I、Nagata Takashi、Katahira Masato
  • Journal Title: Protein Expr Purif Volume: 145 Pages: 45-52
  • DOI: 10.1016/j.pep.2017.12.012
  • Peer Reviewed / Open Access
• [Journal Article] The first successful observation of in-cell NMR signals of DNA and RNA in living human cells (2018)
  • Author(s): Yamaoki Yudai、Kiyoishi Ayaka、Miyake Masayuki、Kano Fumi、Murata Masayuki、Nagata Takashi、Katahira Masato
  • Journal Title: Phys Chem Chem Phys Volume: 20 Issue: 5 Pages: 2982-2985
  • DOI: 10.1039/c7cp05188c
  • NAID: 130007557810
  • Peer Reviewed / Open Access
• [Journal Article] The C-terminal cytidine deaminase domain of APOBEC3G itself undergoes intersegmental transfer for a target search, as revealed by real-time NMR monitoring (2018)
  • Author(s): Kamba Keisuke、Nagata Takashi、Katahira Masato
  • Journal Title: Phys Chem Chem Phys Volume: 20 Issue: 5 Pages: 2976-2981
  • DOI: 10.1039/c7cp05171a
  • Peer Reviewed / Open Access
• [Journal Article] Influence of the DNA sequence/length and pH on deaminase activity, as well as the roles of the amino acid residues around the catalytic center of APOBEC3F (2018)
  • Author(s): Wan Li、Nagata Takashi、Katahira Masato
  • Journal Title: Phys Chem Chem Phys Volume: 20 Issue: 5 Pages: 3109-3117
  • DOI: 10.1039/c7cp04477a
  • Peer Reviewed / Open Access
• [Journal Article] Conjugation of two RNA aptamers improves binding affinity to AML1 Runt domain (2017)
  • Author(s): Nomura Yusuke、Yamazaki Kaori、Amano Ryo、Takada Kenta、Nagata Takashi、Kobayashi Naohiro、Tanaka Yoichiro、Fukunaga Junichi、Katahira Masato、Kozu Tomoko、Nakamura Yoshikazu、Haishima Yuji、Torigoe Hidetaka、Sakamoto Taiichi
  • Journal Title: J Biochem Volume: 162 Issue: 6 Pages: 431-436
  • DOI: 10.1093/jb/mvx049
  • NAID: 40021421327
  • Peer Reviewed / Open Access
• [Journal Article] Observation by Real-Time NMR and Interpretation of Length- and Location-Dependent Deamination Activity of APOBEC3B (2017)
  • Author(s): Wan Li、Nagata Takashi、Morishita Ryo、Takaori-Kondo Akifumi、Katahira Masato
  • Journal Title: ACS Chem Biol Volume: 12 Issue: 11 Pages: 2704-2708
  • DOI: 10.1021/acschembio.7b00662
  • Peer Reviewed / Open Access
• [Journal Article] Backbone and side chain assignments of the second RNA-binding domain of Musashi-1 in its free form and in complex with 5-mer RNA (2017)
  • Author(s): Iwaoka Ryo、Nagata Takashi、Tsuda Kengo、Imai Takao、Okano Hideyuki、Kobayashi Naohiro、Katahira Masato
  • Journal Title: Biomol NMR Assign Volume: 11 Issue: 2 Pages: 265-268
  • DOI: 10.1007/s12104-017-9760-9
  • Peer Reviewed / Open Access
• [Journal Article] Structural Insight into the Recognition of r(UAG) by Musashi-1 RBD2, and Construction of a Model of Musashi-1 RBD1-2 Bound to the Minimum Target RNA (2017)
  • Author(s): Iwaoka Ryo、Nagata Takashi、Tsuda Kengo、Imai Takao、Okano Hideyuki、Kobayashi Naohiro、Katahira Masato
  • Journal Title: Molecules Volume: 22 Issue: 7 Pages: 1207-1207
  • DOI: 10.3390/molecules22071207
  • NAID: 120006492019
  • Peer Reviewed / Open Access
• [Journal Article] Development of an RNA aptamer that acquires binding capacity against HIV-1 Tat protein via G-quadruplex formation in response to potassium ions (2017)
  • Author(s): Yamaoki, Y., Nagata, T., Mashima, T., Katahira, M.
  • Journal Title: Chemical communications Volume: 53 Issue: 52 Pages: 7056-7059
  • DOI: 10.1039/c7cc03312e
  • Peer Reviewed / Open Access
• [Presentation] Structural and functional study of the G-quadruplex anti-prion RNA aptamer and in-cell NMR of nucleic acids (2020)
  • Author(s): Nagata Takashi
  • Organizer: International IPR Seminar, Open up a new era of structural biology with advanced NMR study
  • Int'l Joint Research / Invited
• [Presentation] Development of anti-prion RNA aptamers and destruction of the Alzheimer's disease-related complex (2020)
  • Author(s): Mashima Tsukasa、Iida Mamiko、Lee Joon-Hwa、Yamaoki Yudai、So Masatomo、Kamatari Yuji O.、Hayashi Tomohiko、Kinoshita Masahiro、Kuwata Kazuo、Nagata Takashi、Katahira Masato
  • Organizer: The 46th International Symposium on Nucleic Acids Chemistry, The 3rd Annual Meeting of Japan Society of Nucleic Acids Chemistry
  • Int'l Joint Research
• [Presentation] Heterologous expression and oxidative activity of four fungal ligninolytic enzymes by Pichia pastoris (2020)
  • Author(s): Kenneth TSK, Kondo K, Nagata T, Katahira M
  • Organizer: 日本農芸化学会2020年度大会
• [Presentation] 選択的白色腐朽菌が産する溶解性多糖モノオキシゲナーゼの構造および活性の解析 (2020)
  • Author(s): 近藤 敬子, グエン フエン, ワンサミハルジャ アンドリー, 小瀧 努, 三上 文三, 渡辺 隆司, 永田 崇, 片平 正人
  • Organizer: 日本農芸化学会2020年度大会
• [Presentation] リグニン・多糖複合体分解に関与する白色腐朽菌由来グルクロノイルエステラーゼの速度論解析および添加剤耐性 (2020)
  • Author(s): 坂井勇太, 林孟宜, 檜山明穂, 米沢柚香, 近藤敬子, 永田崇, 片平正人
  • Organizer: 日本農芸化学会2020年度大会
• [Presentation] In-cell NMR法を用いた非標準構造を形成する核酸の構造安定性の解析 (2020)
  • Author(s): 阪本知樹, 山置佑大, 加納ふみ, 村田昌之, 永田崇, 片平正人
  • Organizer: 第100回日本化学会年会
• [Presentation] リグニンオリゴマーモデルとセルラーゼ糖質結合モジュール間の相互作用を原子・分子レベルで可視化するためのNMR解析 (2020)
  • Author(s): 徳永 有希, 永田 崇, 近藤 敬子, 片平 正人, 渡辺 隆司
  • Organizer: 第70回日本木材学会大会
• [Presentation] Oxidative activity of four heterologously expressed fungal ligninolytic enzymes by Pichia pastoris (2020)
  • Author(s): Kenneth TSK, Kondo K, Nagata T, Katahira M
  • Organizer: 第70回日本木材学会大会
• [Presentation] STRUCTURAL AND PHYSICAL BASIS FOR THE HIGHER AFFINITY TO ONCOPROTEIN MDM2 OF A PEPTIDE SELECTED WITH MRNA DISPLAY OVER TUMOR SUPPRESSOR P53 (2020)
  • Author(s): Nagata Takashi、Yamada Tatsuya、Hayashi Tomohiko、Hikiri Shimon、Kobayashi Naohiro、Ikeguchi Mitsunori、Katahira Masato、Kinoshita Masahiro、Yanagawa Hiroshi
  • Organizer: 64th Annual Meeting of the Biophysical Society
  • Int'l Joint Research
• [Presentation] AFMで測定したDNA粒子の特徴抽出と機械学習による四重鎖判別法の開発 (2019)
  • Author(s): 田中陽一郎, 永田崇, 萩野俊郎
  • Organizer: 第42回日本分子生物学会年会
• [Presentation] NADPH再生系を内包するハイブリッド型アゾレダクターゼの開発 (2019)
  • Author(s): 堀内正隆, 永田崇, 片平正人, 小橋川敬博, 鈴木定彦, 落合正則
  • Organizer: 第42回日本分子生物学会年会
• [Presentation] Promoter associated non-coding RNA, pncRNA, can diminish the aggregation of TLS caused by shearing stress (2019)
  • Author(s): Hamad NH, Mashima T, Yamaoki Y, Kondo K, Yoneda R, Kurokawa R, Nagata T, Katahira, M
  • Organizer: 第42回日本分子生物学会年会
• [Presentation] HIV Vifによるユビキチン化を介さないヒトAOBEC3G活性の阻害 (2019)
  • Author(s): 神庭圭佑, 万里, 雲財悟, 森下了, 永田崇, 片平正人
  • Organizer: 第42回日本分子生物学会年会
• [Presentation] Vif - CBFβ - CUL5 - ELOB - ELOC 複合体に結合するアプタマーの解析 (2019)
  • Author(s): 熊谷紀志, 鈴木拓也, 関川湧斗, 神庭圭介, 万里, 永田佳代子, 高折晃史, 片平正人, 永田崇, 坂本泰一
  • Organizer: 第42回日本分子生物学会年会
• [Presentation] 選択的白色腐朽菌に由来するLytic polysaccharide monooxygenaseのX線結晶構造および活性の解析 (2019)
  • Author(s): 近藤敬子, Nuyen Nguyen, Andry Wangsamihardja, 小瀧努, 三上文三, 渡辺隆司, 永田崇, 片平正人
  • Organizer: 第42回日本分子生物学会年会
• [Presentation] In-cell NMR法によるヒト生細胞内の核酸の構造およびダイナミクスの解析 (2019)
  • Author(s): 山置佑大, 永田崇, 阪本知樹, 高見昇平, 加納ふみ, 村田昌之, 片平正人
  • Organizer: 第42回日本分子生物学会年会
• [Presentation] ヒト生細胞内における核酸の非標準構造および構造安定性のin-cell NMR法を用いた解析 (2019)
  • Author(s): 阪本知樹, 山置佑大, 加納ふみ, 村田昌之, 永田崇, 片平正人
  • Organizer: 第42回日本分子生物学会年会
• [Presentation] In-cell NMR法によるヒト生細胞内における核酸‐リガンド相互作用の解析 (2019)
  • Author(s): 高見昇平, 山置佑大, 加納ふみ, 村田昌之, 坂本秦一, 原田和雄, 永田崇, 片平正人
  • Organizer: 第42回日本分子生物学会年会
• [Presentation] In-cell NMR法を用いたヒト生細胞内のDNAおよびRNAの構造安定性評価 (2019)
  • Author(s): 山置佑大, 永田崇, 高見昇平, 阪本知樹, 片平正人
  • Organizer: 第58回NMR討論会(2019)(NMR討論会・SEST2019連合大会)
• [Presentation] 非常に高い活性を有する抗プリオンRNAアプタマーの構造機能相関 (2019)
  • Author(s): 永田崇, 真嶋司, Lee Joon-Hwa, 鎌足雄司, 林智彦, 木下正弘, 桑田一夫, 片平正人
  • Organizer: 第58回NMR討論会(2019)(NMR討論会・SEST2019連合大会)
• [Presentation] The observation of in-cell NMR signals of nucleic acids in living human cells (2019)
  • Author(s): Yamaoki Y, Kiyoishi A, Miyake M, Lin KH, Kano F, Murata M, Nagata T, Katahira M
  • Organizer: 7th International Meeting of Quadruplex Nucleic Acids
  • Int'l Joint Research
• [Presentation] In-cell NMR法を用いたヒト生細胞内核酸の構造およびダイナミクスの評価 (2019)
  • Author(s): 山置佑大, 永田崇, 清石 彩華, 三宅 雅之, 加納ふみ, 村田昌之, 片平正人
  • Organizer: 第57回日本生物物理学会年会
• [Presentation] HIV Vif-ヒトE3ユビキチンリガーゼ複合体によるヒト抗ウイルス因子APOBEC3Gの脱アミノ化阻害の分子メカニズム (2019)
  • Author(s): 神庭圭佑, 万里, 雲財悟, 森下了, 永田崇, 片平正人
  • Organizer: 第57回日本生物物理学会年会
• [Presentation] The effect of the distance between the RNA sequences recognized by two RNA-binding domains on the affinity of the MSI1-RNA interaction (2019)
  • Author(s): Tu Wei Hsm, 神庭 圭佑, 永田 崇, 片平 正人
  • Organizer: 第57回日本生物物理学会年会
• [Presentation] NMR Analysis for Molecular Insight into the Interaction between Lignin and Carbohydrate Binding Module of Cel7A from Trichoderma reesei (2019)
  • Author(s): Tokunaga, Y, Nagata, T, Kondo, K, Katahira, M, Watanabe, T
  • Organizer: The 20th International Symposium on Wood, Fiber and Pulping Chemistry
  • Int'l Joint Research
• [Presentation] Structural analyses of covalent linkages between lignin and hemicellulose in wood cell walls (2019)
  • Author(s): H. Nishimura, K. Nagata, M. Yamada, T. Nagata, M. Katahira, T. Watanabe
  • Organizer: 1st International Lignin Symposium
  • Int'l Joint Research
• [Presentation] NMR study on Binding Sites of 13C-Lignin Oligomer Models with Carbohydrate (2019)
  • Author(s): Tokunaga, Y, Nagata, T, Kondo, K, Katahira, M, Watanabe, T
  • Organizer: 1st International Lignin Symposium
  • Int'l Joint Research
• [Presentation] Strategy of lignocellulose conversion using catalysts with controlled affinity to lignin (2019)
  • Author(s): Watanabe, T, Tokunaga, Y, Oshiro, S, Saito, K, Okano, H, Nishimura, H, Nagata, T, Kondo, K, Katahira, M, Isozaki, K, Takaya, H, Nakamura, M
  • Organizer: 1st International Lignin Symposium
  • Int'l Joint Research
• [Presentation] セルラーゼ糖質結合モジュールに対するリグニンモデル吸着部位の解析 (2019)
  • Author(s): 德永有希, 永田崇, 近藤敬子, 片平正人, 渡辺隆司
  • Organizer: 第33回セルラーゼ研究会
• [Presentation] Disruption of the Alzheimer’s disease-related complex between prion and amyloid β by an anti-prion aptamer (2019)
  • Author(s): T. Mashima, Y. M. Iida, Yamaoki, M. So, T. Nagata, M. Katahira
  • Organizer: Commemorative International Symposium of the Japan Society of Nucleic Acids Chemistry (CISNAC) 2019
  • Int'l Joint Research
• [Presentation] In-cell NMR法を用いたヒト生細胞内の核酸の構造と安定性の評価 (2019)
  • Author(s): 山置佑大, 永田崇, 清石彩華, 三宅雅之, Lin Kuan-Heng, 高見昇平, 加納ふみ, 村田昌之, 片平正人
  • Organizer: 第21回日本RNA学会年会
• [Presentation] プリオンタンパク質の構造変換を抑制するRNAアプタマーの開発と構造決定 (2019)
  • Author(s): 真嶋司, Lee Joon-Hwa, 鎌足雄司, 林智彦, 永田崇, 西川富美子, 西川諭, 木下正弘, 桑田一夫, 片平正人
  • Organizer: 第21回日本RNA学会年会
• [Presentation] An insight into the target search and HIV Vif-dependent inactivation of APOBEC3F and APOBEC3G elucidated by tracking the deamination events (2019)
  • Author(s): Kamba K, Wan L, Nagata T, Katahira M
  • Organizer: 8th Asia-Pacific NMR Symposium
  • Int'l Joint Research
• [Presentation] APOBEC3FとAPOBEC3Gの脱アミノ化速度は、それら及びDNAの濃度、さらにDNAの長さに影響を受ける (2019)
  • Author(s): 神庭圭佑, 万里, 永田崇, 片平正人
  • Organizer: 第19回日本蛋白質科学会年会 第71回日本細胞生物学会大会 合同年次大会
• [Presentation] セルラーゼ糖質結合モジュールとリグニン間相互作用部位のNMRによる解析 (2018)
  • Author(s): 徳永有希、大城理志、永田崇、近藤敬子、西村裕志、片平正人、渡辺隆司
  • Organizer: 第68回日本木材学会
• [Presentation] Modeling of the tandem RNA-binding domains of a neural and oncogenic protein, Musashi1, engaging the minimum target RNA (2017)
  • Author(s): Ryo Iwaoka, Takashi Nagata, Kengo Tsuda, Takao Imai, Hideyuki Okano, Naohiro Kobayashi, Masato Katahira
  • Organizer: 第40回日本分子生物学会年会
• [Presentation] The effect of different target nucleic acids on the structural change of TLS (2017)
  • Author(s): Nesreen Hamad, Tsukasa Mashima, Hiroki Watanabe, Takayuki Uchihashi, Riki Kurokawa, Takashi Nagata, Masato Katahira
  • Organizer: 第40回日本分子生物学会年会
• [Presentation] Studies on lytic polysaccharide monooxygenase (LPMO) from the selective white rot fungus, Ceriporiposis subvermispora (2017)
  • Author(s): Yuu Iseki, Satoshi Oshiro, Takashi Nagata, Keiko Kondo, Hiroshi Nishimura, Masato Katahira, Takashi Watanabe
  • Organizer: Humanosphere Science School 2017, Salak Tower (Bogor, Indonesia), 2017.11.1-2
  • Int'l Joint Research
• [Presentation] Analysis of Adsorption Mechanism between Carbohydrate Binding Module of Cellulase and Lignin by NMR (2017)
  • Author(s): Yuki Tokunaga, Satoshi Oshiro, Takashi Nagata, Keiko Kondo, Hiroshi Nishimura, Masato Katahira, Takashi Watanabe
  • Organizer: Humanosphere Science School 2017, Salak Tower (Bogor, Indonesia), 2017.11.1-2
  • Int'l Joint Research
• [Presentation] Analysis of Lignin-Carbohydrate Complexes in Woody Biomass by Using NMR (2017)
  • Author(s): Shizuka Sakon, Hiroshi Nishimura, Misato Yamada, Yukari Ohta, Keiko Kondo, Yudai Yamaoki, Takashi Nagata, Masato Katahira, Takashi Watanabe
  • Organizer: Humanosphere Science School 2017, Salak Tower (Bogor, Indonesia), 2017.11.1-2
  • Int'l Joint Research
• [Presentation] 翻訳開始前複合体において形成される翻訳開始因子間の結合ネットワークの役割 (2017)
  • Author(s): 永田崇、吉田尚志、雲財悟、浦野健、浅野桂、尾林栄治
  • Organizer: 第56回NMR討論会
• [Presentation] In-cell NMR法によるヒト細胞内DNAおよびRNAの観測 (2017)
  • Author(s): 山置 佑大, 清石 彩華, 三宅雅之, 加納 ふみ, 村田 昌之, 永田 崇, 片平 正人
  • Organizer: 第56回NMR討論会
• [Presentation] RNA結合タンパク質Musashi-1のRBD2:r(GUAGU)複合体溶液構造決定と標的RNA選択メカニズムの解明 (2017)
  • Author(s): 岩岡諒，永田崇，津田健吾，今井貴雄，岡野栄之，小林直宏，片平正人
  • Organizer: 第56回NMR討論会
• [Presentation] Characterization of the cytidine deamination properties of human APOBEC3B by real-time NMR, which are quite different from those of APOBEC3G (2017)
  • Author(s): Li Wan, Takashi Nagata, Ryo Morishita, Akifumi Takaori-Kondo, Masato Katahira
  • Organizer: The 44th International Symposium on Nucleic Acids Chemistry, The 1st Annual Meeting of Japan Society of Nucleic Acids Chemistry
  • Int'l Joint Research
• [Presentation] 深層学習により強化されたNMR立体構造解析 (2017)
  • Author(s): 小林直宏、永田崇、服部良一、新家粧子、Julia M. Wurz、Peter Guntert、児嶋長次郎、藤原敏道
  • Organizer: 第56回NMR討論会
• [Presentation] Analysis of peroxidase activity, lignin model degradation of heterologous expressed soluble fungal ligninolytic enzymes (2017)
  • Author(s): Meng-I Lin, Keiko Kondo, Takashi Nagata, Masato Katahira
  • Organizer: 2017 Taiwan-Japan Biomedical Symposium on Magnetic Resonance
  • Int'l Joint Research
• [Presentation] Sphingombium sp. YG1由来guaiacylglycerol-β-guaiacyl ether - Cα位酸化酵素の酵素学的解析と利用 (2017)
  • Author(s): 前田亜鈴悠、宋晶、西真郎、黒澤佳奈子、市川淳子、 塩谷美夏、山置佑大、永田崇、片平正人、山田美沙登、渡辺隆司、大田ゆかり
  • Organizer: 第62回リグニン討論会
• [Presentation] フラグメント分子軌道法を活用したリグニンβ-O-4還元開裂酵素の機能改変 (2017)
  • Author(s): 嶋根康弘、黒澤佳奈子、市川淳子、前田亜鈴悠、布浦拓郎、山置佑大、永田崇、片平正人、高谷光、吉田亮太、中村正治、渡辺隆司、大田ゆかり
  • Organizer: 第62回リグニン討論会
• [Presentation] Kinetic studies of the two fungal glutathione-dependent enzymes and their potential roles in the intracellular detoxification and wood-derived compound metabolism (2017)
  • Author(s): Wan Hasnidah Wan Osman, Meng-I Lin, Keiko Kondo, Takashi Nagata, Masato Katahira
  • Organizer: 第62回リグニン討論会
• [Presentation] Development of the artificial multi-domain enzymes immobilized on the curdlan sheet at low cost. (2017)
  • Author(s): Masataka Horiuchi, Takashi Nagata, Masato Katahira, Yoshihiro Kobashigawa, Yasuhiko Suzuki, Masanori Ochiai
  • Organizer: The 8th International Symposium of Advanced Energy Science
  • Int'l Joint Research
• [Presentation] Structure-function relationship of Epstein-Barr Virus EBNA3C NLS and transport receptor (2017)
  • Author(s): Go Matsuda, Takashi Nagata, Masato Katahira, Ken-ichi Imadome
  • Organizer: The 8th International Symposium of Advanced Energy Science
  • Int'l Joint Research
• [Presentation] Development of RNA binding peptides based on tertiary structural information (2017)
  • Author(s): Natsuki Watanabe, Tomoaki Kinase, Takashi Nagata, Masato Katahira, Kazuo Harada, and Taiichi Sakamoto
  • Organizer: The 8th International Symposium of Advanced Energy Science
  • Int'l Joint Research
• [Presentation] テロメア長短縮をもたらすTLS/FUS蛋白質とテロメアDNAおよびTERRAのグアニン四重鎖との複合体に関するNMR解析 (2017)
  • Author(s): 近藤 敬子、 真嶋 司、大吉 崇文、黒川 理樹、小林直宏、永田 崇、片平 正人
  • Organizer: 第55回生物物理学会
• [Presentation] Influences of length and concentration of the DNA substrate, as well as the location of the target sequence, on deamination by APOBEC3F (2017)
  • Author(s): Li Wan, Takashi Nagata, Masato Katahira
  • Organizer: 第55回生物物理学会
• [Presentation] An anti-prion aptamer inhibits the formation of prion protein-amyloid β oligomer complex that is related to Alzheimer's disease (2017)
  • Author(s): Mamiko IIDA, Tsukasa MASHIMA, Yudai YAMAOKI, Takashi NAGATA, Masato KATAHIRA
  • Organizer: 第55回生物物理学会
• [Presentation] 18-crown-6を用いるヒドロニウム溶媒和イオン液体の基礎物性 (2017)
  • Author(s): 北田敦、竹岡駿、近都康平、深見一弘、才村正幸、永田崇、片平正人、邑瀬邦明
  • Organizer: 2017年電気化学会秋季大会
• [Presentation] TLS/FUSの非コードRNAの認識機構の構造学的研究 (2017)
  • Author(s): 真嶋司, 小澤 駿介, Nesreen Hamad, 米田竜馬, 黒川 理樹, 永田崇, 片平 正人
  • Organizer: 第19回日本RNA学会年会
• [Presentation] Conformational change detection of translocated in liposarcoma, TLS, upon binding to various RNAs (2017)
  • Author(s): Nesreen Hamad, Tsukasa Mashima,Yudai Yamaoki, Keiko Kondo, Takashi Nagata, Masato Katahira
  • Organizer: 第19回日本RNA学会年会
• [Presentation] NMR法によるセルラーゼ糖質結合モジュール-リグニン間相互作用の解明 (2017)
  • Author(s): 徳永有希、大城理志、永田崇、近藤敬子、西村裕志、片平正人、渡辺隆司
  • Organizer: 第３１回セルラーゼ研究会
• [Presentation] NMR Analysis of Interaction Mechanism between Carbohydrate Binding Module of Cellulase and Lignin (2017)
  • Author(s): Yuki Tokunaga, Satoshi Oshiro, Keiko Kondo, Takashi Nagata, Hiroshi Nishimura, Masato Katahira, Takashi Watanabe
  • Organizer: The 2nd Asia Research Node Symposium on Humanosphere Science
  • Int'l Joint Research
• [Presentation] HIV-1 RRE由来のRNA断片と人工ペプチドの相互作用の解析 (2017)
  • Author(s): 渡邊 なつき，木名瀬智章，永田崇，片平正人，原田和雄，坂本泰一
  • Organizer: 平成29年度日本生化学会関東支部例会
• [Presentation] TLS/FUSのRNA認識機構と結合に伴うコンフォーメーション変化の研究 (2017)
  • Author(s): 真嶋 司, 小澤 駿介, Nesreen Hamad, 米田 竜馬, 黒川 理樹, 永田 崇, 片平 正人
  • Organizer: 第17回日本蛋白質科学会年会
• [Presentation] High yield production, activity analysis, and homologous modeling of soluble fungal peroxidases expressed in E. coli (2017)
  • Author(s): Meng-I Lin, Keiko Kondo, Takashi Nagata, Masato Katahira
  • Organizer: 第17回日本蛋白質科学会年会
• [Presentation] APOBEC3Gのストランド間移動の実時間NMR法検出 (2017)
  • Author(s): 神庭圭佑，永田崇，片平正人
  • Organizer: 第17回日本蛋白質科学会年会
• [Presentation] Highly automated NMR signal assignments using peak-noise recognition assisted by Deep Neural Networks (2017)
  • Author(s): Kobayashi, N., Nagata, T., Kojima, C., and Fujiwara, T.
  • Organizer: Gordon Research Conference. Computational Aspects-Biomol NMR
  • Int'l Joint Research