Elsevier

Genomics

Volume 98, Issue 4, October 2011, Pages 280-287
Genomics

Genome-wide profiling of DNA methylation in human cancer cells

https://doi.org/10.1016/j.ygeno.2011.07.003Get rights and content
Under an Elsevier user license
open archive

Abstract

Global changes in DNA methylation correlate with altered gene expression and genomic instability in cancer. We have developed a methylation-specific digital sequencing (MSDS) method that can assess DNA methylation on a genomic scale. MSDS is a simple, low-cost method that combines the use of methylation-sensitive restriction enzymes with second generation sequencing technology. DNA methylation in two colon cancer cell lines, HT29 and HCT116, was measured using MSDS. When methylation levels were compared between the two cell lines, many differentially methylated regions (DMRs) were identified in CpG island shore regions (located within 2 kb of a CpG island), gene body regions and intergenic regions. The number of DMRs in the vicinity of gene transcription start sites correlated with the level of expression of TACC1, CLDN1, and PLEKHC1 (FERMT2) genes, which have been linked to carcinogenesis. The MSDS method has the potential to provide novel insight into the functional complexity of the human genome.

Keywords

DNA methylation
Second generation sequencing technology
Differentially methylated regions
Colon cancer

Cited by (0)

1

Fax: + 81 3 5841 3393.

2

Fax: + 81 4 7136 3977.

3

Fax: + 81 4 7136 4080.