ゲノムの複製終結と分配制御

• 研究課題/領域番号: 04256103
• 研究種目: 重点領域研究
• 配分区分: 補助金
• 研究機関: 熊本大学
• 研究代表者: 平賀 壮太 (平賀 壯太) 熊本大学, 医学部, 教授 (40027321)
• 研究分担者: 小椋 光 熊本大学, 医学部, 助教授 (00158825) ; 西村 昭子 国立遺伝学研究所, 助教授 (20142002) ; 加藤 潤一 東京大学, 医科学研究所, 助手 (10194820) ; 永井 和夫 東京工業大学, 生命理工学部, 教授 (00011974) ; 岡崎 恒子 名古屋大学, 理学部, 教授 (10022584) ; 丹羽 修身 京都大学, 理学部, 助教授 (70144318)
• 研究期間 (年度): 1994
• 研究課題ステータス: 完了 (1994年度)
• 配分額: 166,000千円 (直接経費: 166,000千円); 1994年度: 64,000千円 (直接経費: 64,000千円); 1993年度: 61,000千円 (直接経費: 61,000千円); 1992年度: 41,000千円 (直接経費: 41,000千円)
• キーワード: MukB蛋白 / トポイソメラーゼ / FtsH蛋白 / ヒト染色体 / セントロメア / アルフォイドDNA / ミニ染色体 / 熱ショック転写因子 / 分裂酵母

研究概要

1.大腸菌染色体分配に関与するmukB遺伝子の上流に存在するofr50の破壊株を解析して、この遺伝子(mukFと命名)もmkB遺伝子と同じく染色体分配に関与することを明らかにした(平賀)。
2.ヒト染色体セントロメア領域に存在するアルフォイドDNA中のCEN-B-BoxとCEN-B蛋白の複合体の高次構造について解析した(岡崎)。
3.トポイソメラーゼIIαとIIβの両方の活性阻害をする抗体を注入すると細胞は中記まで進がそれ以後は進まないことを明らかにした(菊池)。
4.大腸菌のトポイソメラーゼIV(parCとparE)と相互作用をする蛋白質を検索することを目標として、dominant negativeなparC変異を抑制する多重コピー抑制遺伝子を多数分離し、解析した(加藤)。
5.FtsH蛋白は熱ショック転写因子δ32を分解するATP、Zn++依存性の新しいタイプの蛋白分解酵素であることを明らかにした(小椋)。
6.DNA合成阻害時においても細胞分裂の阻害が起こりにくいcfcA、cfcB変異がそれぞれglyS、apaHの変異であることを明らかにし、細胞内のAppppAの量が細胞分裂の頻度を調節していることを明らかにした(西村昭子)。
7.Fプラスミドの分配遺伝子sopBの欠失が宿主細胞の増殖を阻害する現象を解析して、この増殖阻害現象はsopB欠失のミニFプラスミドでは見られないが、このミニFプラスミドに30〜40kb以上の長いDNA断片を挿入すると強く表れることを明らかにした(三木)。

研究成果

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