神経内分泌細胞における酵素活性化物質の生理的意義

• 研究課題/領域番号: 07670036
• 研究種目: 基盤研究(C)
• 配分区分: 補助金
• 応募区分: 一般
• 研究分野: 解剖学一般(含組織学・発生学)
• 研究機関: 昭和大学
• 研究代表者: 中井 康光 昭和大学, 医学部, 教授 (60053807)
• 研究分担者: 王 慶平 昭和大学, 医学部, 助手 (10245821) ; 塩田 清二 昭和大学, 医学部, 講師 (80102375) ; 落合 英彦 昭和大学, 医学部, 助教授 (40053954) ; 内藤 延子 昭和大学, 医学部, 助教授 (30053903)
• 研究期間 (年度): 1995 – 1996
• 研究課題ステータス: 完了 (1996年度)
• 配分額: 2,300千円 (直接経費: 2,300千円); 1996年度: 900千円 (直接経費: 900千円); 1995年度: 1,400千円 (直接経費: 1,400千円)
• キーワード: 視床下部 / 神経分泌細胞 / PACAPニューロン / グルタミン酸ニューロン / NOSニューロン / レセプター / シナプス / 免疫電顕的観察 / 神経分泌ニューロン / PACAP / PACAPレセプター / nitric oxide synthase / グルタミン酸 / グルタミン酸レセプター / 免疫電顕

研究概要

神経内分泌細胞におけるpituitary adenylate cyclase activating polypetide(PACAP),nitric oxide synthase(NOS),pおよびグルタミン酸(Glu)とそのレセプターであるN-methyl-D-aspartate(NMDA)の機能的意義を追求するために,上記酵素活性化物質ならびにそれらの関連物質の超微局在を免疫電顕的に観察し以下のような結果を得た.
PACAPP様免疫陽性軸索終末がラット視床下部の視索上核や室傍核に多数分布し,神経内分泌細胞の細胞体やその突起にシナプスを形成しているのが観察された.またPACAレセプター(PACAP-R)様免疫陽性反応は視床下部全域に点状に分布し,特に視索上核や室傍核に多数みられた.免疫電顕でPACAP-R様免疫反応は神経内分泌細胞の形質膜とくにシナプス形成部位のシナプス後膜にみられた.以上の結果から,PACAP含有ニューロンは視床下部の神経分泌ニューロンにシナプスを介して直接神経支配している可能性が示唆された.
NOSニューロンについては背側縫線核領域で観察し,NOSニューロンはセロトニン・ニューロンとよく似た分布を示し,NOS様免疫陽性終末が他のニューロンにシナプスを形成している所見を得た.視床下部領域におけるNOSニューロンの詳細については現在観察中である.
GluニューロンとNMDA型Gluレセプター(MNDA-R1)について免疫電顕的に観察し,ラット視床下部室周囲核において,NMDA-R1免疫陽性突起にGlu免疫陽性およびGlu免疫陰性の両方の軸索終末がシナプスを形成しているのを観察した.そのほかに,GABA様免疫陽性終末がNMDA-R1様免疫陽性樹状突起にシナプスを形成しているのも観察された.以上から,NMDA-R1含量ニューロンが興奮性および抑制性アミノ酸ニューロンによって神経支配されたいることが明らかになった.

研究成果

• [文献書誌] Qing-Ping Wang: "Electron Microscopic Study of GABAergic Synaptic Innervation of Nitric Oxide Synthase Immunoreactive Neurons in the Dorsal Raphe Nucleus in the Rat." Synapse. 25. 24-29 (1997)
  • 説明: 「研究成果報告書概要(和文)」より
• [文献書誌] Daigaku Uchida: "Prevention of ischemia-induced death of hippocampal neurons by pituitary adenylate cyclase activating plypeptide." Brain Research. 736. 280-286 (1996)
  • 説明: 「研究成果報告書概要(和文)」より
• [文献書誌] Seiji Shioda: "Pituitary Adenylate Cyclase Activating Plypeptide and Its Type I Receptors in the Rat Hypothalamus:Neuroendocrine Interaction." Annals of New York Academy of Sciences. 805. 670-676 (1996)
  • 説明: 「研究成果報告書概要(和文)」より
• [文献書誌] Seiji Shioda: "Localization of pituitary Adenylate Cyclase-Activating Plypeptide and Its Type I Receptor in the Rat Ovary:Immunohistochemistry and in situ Hybridization." Annals of New York Academy of Sciences. 805. 677-683 (1996)
  • 説明: 「研究成果報告書概要(和文)」より
• [文献書誌] Seiji Shioda: "Direct projections from catecholaminergic neurons in the caudal ventrolateral medulla to vasopressin containing neurons in the supraoptic nucleus:a triple-labeling electron microscope study in the rat." Neuroscience Letters. 221. 45-48 (1996)
  • 説明: 「研究成果報告書概要(和文)」より
• [文献書誌] Qing-Ping Wang: "Electron microscopic study of GABAergic synaptic innervation of neurotensin-immunoreactive neurons in the dorsal raphe nucleus." Brain Research. 730. 118-124 (1996)
  • 説明: 「研究成果報告書概要(和文)」より
• [文献書誌] Qing-Ping Wang: "Double preembedding immunostaining for the study by electron microscopy of synaptic relationships between immunocytochemically identified neurons." Neuroscience Protocols. 050-05. 01-13 (1996)
  • 説明: 「研究成果報告書概要(和文)」より
• [文献書誌] Jian-Lian Guan: "Synaptic Innervation of Enkephalinergic Neurons by Axon Terminals Immunoreactive to Dopamine-β-Hydroxylase in the Rat Area Postrema." Peptides. 17(7). 1203-1206 (1996)
  • 説明: 「研究成果報告書概要(和文)」より
• [文献書誌] Jian-Lian Guan: "GABAergic Synaptic Innervation of Catecholaminergic Neurons in the Area postrema of the Rat." Acta Anatomica. 156. 46-52 (1996)
  • 説明: 「研究成果報告書概要(和文)」より
• [文献書誌] Qing-Ping Wang: "Immunoelectron microscopy of β-endorphinergic synaptic innervation of nitric oxide synthase immunoreactive neurons in the dorsal raphe nucleus." Brain Research. 684. 185-193 (1995)
  • 説明: 「研究成果報告書概要(和文)」より
• [文献書誌] Hitoshi Nakayama: "Immunocytochemical localization of nicotinic acetylcholine receptor in rat cerebral cortex" Molecular Brain Research. 32. 321-328 (1995)
  • 説明: 「研究成果報告書概要(和文)」より
• [文献書誌] Hidehiko Ochiai: "Immunocytochemical Examination of Catecholaminergic Innervation of Neurosecretory Fibers and Pituicyles in the Rat Neurohypophisis." Showa Univ.J.of Med.Sci.7(2). 183-192 (1995)
  • 説明: 「研究成果報告書概要(和文)」より
• [文献書誌] Jian-Lian Guan: "Reciprocal Synaptic Relations Between Enkephalinergic and GABAergic Neurons in the Area Postrema of the Rat." Brain Research Bulletin. 36(4). 349-354 (1995)
  • 説明: 「研究成果報告書概要(和文)」より
• [文献書誌] Qing-Ping Wang: "Distribution and Synaptic Relations of NOS Neurons in the Dorsal Raphe Nucleus:A Comparison to 5-HT Neurons." Brain Research Bulletin. 37(2). 177-187 (1995)
  • 説明: 「研究成果報告書概要(和文)」より
• [文献書誌] Jian-Lian Guan: "The Reciprocal Synaptic Relations Between Enkephalinergic Neurons and Catecholaminergic Neurons in the Area Postrema." Brain Research Bulletin. 38(5). 461-466 (1995)
  • 説明: 「研究成果報告書概要(和文)」より
• [文献書誌] Qing-Ping Wang: "Synaptic Relations of Neurotensinergic Neurons in the Dorsal Raphe Nucleus." Peptides. 16(8). 1421-1427 (1995)
  • 説明: 「研究成果報告書概要(和文)」より
• [文献書誌] Hitoshi Nakayama: "Immunocytochemical localization of nicotinic acetylcholine receptor in rat cerebral cortex." Molecular Brain Research. 32. 321-328 (1995)
  • 説明: 「研究成果報告書概要(欧文)」より
• [文献書誌] Hidehiko Ochiai: "Immunocytochemical Examination of Catecholaminergic Innervation of Neurosecretory Fibers and Pituicytes in the Rat Neurohypophysis." Showa Univ.J.of Med.Sci.7 (2). 183-192 (1995)
  • 説明: 「研究成果報告書概要(欧文)」より
• [文献書誌] Jian-Lian Guan: "Reciprocal Synaptic Relations Between Enkephalinergic and GABAergic Neurons in the Area Postrema of the Rat." Brain Research Bulletin. 36 (4). 349-354 (1995)
  • 説明: 「研究成果報告書概要(欧文)」より
• [文献書誌] Qing-Ping Wang: "Distribution and Synaptic Relations of NOS Neurons in the Dorsal Raphe Nucleus : A Comparison to 5-HT Neurons." Brain Research Bulletin. 37 (2). 177-187 (1995)
  • 説明: 「研究成果報告書概要(欧文)」より
• [文献書誌] Jian-Lian Guan: "The Reciprocal Synaptic Relations Between Enkephalinergic Neurons and Catecholaminergic Neurons in the Area Postrema." Brain Reserach Bulletin. 38 (5) B. 461-466 (1995)
  • 説明: 「研究成果報告書概要(欧文)」より
• [文献書誌] Qing-Ping Wang: "Synaptic Relations of Neurotensinergic Neurons in the Doral Raphe Nucleus." Peptides. 16 (8). 1421-1427 (1995)
  • 説明: 「研究成果報告書概要(欧文)」より
• [文献書誌] Qing-Ping Wang: "Electron Microscopic Study of GABAergic Synaptic Innervation of Nitric Oxide Synthase Immunoreactive Neurons in the Dorsal Raphe Nucleus in the Rat." Synapse. 25. 24-29 (1997)
• [文献書誌] Daigaku Uchida: "Prevention of ischemia-induced death of hippocampal neurons by pituitary adenylate cyclase activating plypeptide." Brain Research.736. 280-286 (1996)
• [文献書誌] Seiji Shioda: "Pituitary Adenylate Cyclase Activating Plypeptide and Its Type I Receptors in the Rat Hypothalamus : Neuroendocrine Interaction." Annals of New York Academy of Sciences. 805. 670-676 (1996)
• [文献書誌] Seiji Shioda: "Localization of Pituitary Adenylate Cyclase-Activating Plypeptide and Its Type I Receptor in the Rat Ovary : Immunohistochemistry and in situ Hybridization." Annals of New York Academy of Sciences. 805. 677-683 (1996)
• [文献書誌] Seiji Shioda: "Direct projectios from catecholaminergic neurons in the caudal ventrolateral medulla to vasopressin-containing neurons in the supraoptic nucleus : a triple-labeling electron microscope study in the rat." Neuroscience Letters. 221. 45-48 (1996)
• [文献書誌] Qing-Ping Wang: "Electron microscopic study of GABAergic synapic innervation of neurotensin-immunoreactive neuros in the dorsal raphe nucleus." Brain Research. 730. 118-124 (1996)
• [文献書誌] Qing-Ping Wang: "Double preembedding immunostaining for the study by electron microscopy of synaptic relationships between immunocytochemically identified neurons." Neuroscience Protocols. 050-05. 01-13 (1996)
• [文献書誌] Jian-Lian Guan: "Synaptic Innervation of Enkephalinergic Neurons by Axon Terminals Immunoreactive to Dopamine-β-Hydroxylase in the Rat Area Postrema." Peptides. 17(7). 1203-1206 (1996)
• [文献書誌] Jian-Lian Guan: "GABAergic Synaptic Innervation of Catecholaminergic Neurons in the Area postrema of the Rat." Acta Anatomica. 156. 46-52 (1996)
• [文献書誌] Qing-Ping Wang: "Immunoelectron microscopy of β-endorphinergic synaptic innervation of nitric oxide synthase immunoreactive neurons in the dorsal raphe nucleus." Brain Research. 684. 185-193 (1995)
• [文献書誌] Hitoshi Nakayama: "Immunocytochemical localizationof nicotinic acetylcholine receptor in rat cerebral cortex." Molecular Brain Research. 32. 321-328 (1995)
• [文献書誌] Hidehiko Ochiai: "Immunocytochemical Examination of Catecholaminergic Innervation of Neurosecretory Fibers and Pituicytes in the Rat Neurohypophysis." Showa Univ.J.of Med.Sci.7(2). 183-192 (1995)
• [文献書誌] Jian-Lian Guan: "Reciprocal Synaptic Relations Between Enkephalinergic and GABAergic Neurons in the Area Postrema of the Rat." Brain Research Bulletin. 36(4). 349-354 (1995)
• [文献書誌] Qing-Ping Wang: "Distribution and Synaptic Relations of NOS Neurons in the Dorsal Raphe Nucleus : A Comparison to 5-HT Neurons." Brain Research Bulletin. 37(2). 177-187 (1995)
• [文献書誌] Jian-Lian Guan: "The Reciprocal Synaptic Relations Between Enkephalinergic Neurons and Catecholaminergic Neurons in the Area Postrema." Brain Research Bulletin. 38(5). 461-466 (1995)
• [文献書誌] Qing-Ping Wang: "Synaptic Relations of Neurotensinergic Neurons in the Dorsal Raphe Nucleus." Peptides. 16(8). 1421-1427 (1995)
• [文献書誌] Qing-Ping Wang: "Synaptic Relations of Neurotensinergic Neurons in the Dorsal Raphe Nucleus" Peptides. 16. 1421-1427 (1995)
• [文献書誌] Hitoshi Nakayama: "Immunocytochemical localization of nicotinic acetylcholine receptor in rat cerebral cortex" Molecular Brain Research. 32. 321-328 (1995)
• [文献書誌] Qing-Ping Wang: "immunoelectron microscopy of β-endorphinergic synaptic innervation of nitric oxide synthase immunoreactive neurons in the dorsal raphe nucleus" Brain Research. 684. 185-193 (1995)
• [文献書誌] Qing-Ping Wang: "Distribution and Synaptic Relations of NOS Neurons in the Dorsal Raphe Nucleus : A Comparison to 5-HT Neurons" Brain Research Bulletin. 37. 177-187 (1995)
• [文献書誌] Qing-Ping Wang: "Immunoelectron microscopy of enkephalinergic innervation of GABAergic neurons in the periaqueductal gray" Brain Research. 665. 39-46 (1994)
• [文献書誌] Shigeo Nakajo: "Localization of phosphoneuroprotein 14 (PNP 14) and its mRNA expression in rat brain determined by immunocytochemistry and in situ hybridization" Molecular Brain Research. 27. 81-86 (1994)
• [文献書誌] Seiji Shioda: "PACAP and its type 1 receptors in the hypthalamus : Neuroendocrine interaction" Ann. New york Acad. Sci. (in press). (1995)