光による生体内情報リズムの制御機構

• 研究課題/領域番号: 08307002
• 研究種目: 基盤研究(A)
• 配分区分: 補助金
• 応募区分: 一般
• 研究分野: 環境生理学(含体力医学・栄養生理学)
• 研究機関: 京都府立医科大学
• 研究代表者: 井端 泰彦 京都府立医科大学, 医学部, 教授 (10079684)
• 研究分担者: 井上 慎一 山口大学, 理学部, 教授 (10274151) ; 岡村 均 神戸大学, 医学部, 教授 (60158813) ; 千原 和夫 神戸大学, 医学部, 教授 (00107955) ; 本間 さと 北海道大学, 医学部, 助教授 (20142713) ; 貴邑 冨久子 横浜市立大学, 医学部, 教授 (40046066)
• 研究期間 (年度): 1996 – 1997
• 研究課題ステータス: 完了 (1997年度)
• 配分額: 11,800千円 (直接経費: 11,800千円); 1997年度: 5,700千円 (直接経費: 5,700千円); 1996年度: 6,100千円 (直接経費: 6,100千円)
• キーワード: 概日リズム / 視交叉上核 / 遺伝子 / 内分泌 / 行動 / 自律神経 / ゲッ歯類 / ヒト

研究概要

本研究は昨年に続き12名の班員による研究により,生体リズム発現及び同調機構,内分泌リズム,自律神経リズム,ヒトにおけるリズム発現,時間記憶などの研究課題について研究を行ってきた。平成9年度に多電極皿上における培養視交叉上核のリズム解析(井端),行動リズム位相変化と哺乳類時間遺伝子(岡村),視交叉上核VIP,AVPニューロンの自律神経反応と高血糖反応への影響(永井),視交叉上核におけるリズム同調機構に対するCREB,CREMの関与(井上)マウスにおける概日リズム突然変異体の分離(海老原),概日リズム光同調に対する心理的ストレスの影響(柴田)GnRHの概日リズム発現に対する視交叉上核AVP,VIPニューロンの影響(貴邑)視床下部成長ホルモン分泌制御機構(千原),条件恐怖刺激に対する視床下部オキシトシン,バソプレシン分泌反応に対する視交叉上核の関与(八木),ヒトにおける生物時間同調因子について(本間),高血圧における血圧の概日リズム機構異常とその治療(田村),睡眠覚醒障害に対する高照度光治療(佐々木)についてそれぞれ研究を行い昨年12月に班会議を開催し研究成果の発表と討論が行われた。特筆すべきことは昨年哺乳動物(ヒトにおいても)にショウジョウバエの時計遺伝子とホモローグである遺伝子が存在することが異なる研究施設から時を同じくして発表されたが(Science,Nature)本研究班の一人である岡村はこの研究グループのひとりであり,彼は続いてこの遺伝子のマウス視交叉上核での発現や光照射による影響や位相変化について"Cell"に発表したことである。即ち哺乳動物における概日リズム発現機序の手がかりが得られたことは大きな成果と考えられる。

研究成果

• [文献書誌] Kawakami,F.: "Loss of-day-night differences in VIP mRNA levels in the suprachiasmatic nucleus of aged rats" Neurosci.Lett.222. 99-102 (1997)
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• [文献書誌] Tanaka,M.: "Direct retinal projections to GRP neurons to GRP neurons in the suprachiasmatic nucleus of the rat" NeuroReport. 8. 2187-2137 (1997)
• [文献書誌] Tanaka,M.: "Peptide Expression in GABAergic Neurons in rat suprachiasmatic nucleus in comparison with other forebrain structures : A double labeling insitu hybridization study." The Journal of Histochemistry & Cytochemistry. 45・9. 1231-1237 (1997)
• [文献書誌] Tamada,Y.: "Neuron-glia interaction in the suprachiasmatic nucleus : A double labeling light and electron" Brain Res.Bull.45・3. 61-67 (1998)
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• [文献書誌] Uemura,T.: "Effects of noradrenaline on GnRH-secreting immortalized hypothalamic (GT1-7) neurons." Endocr J. 44. 73-8 (1997)
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• [文献書誌] Nakamura,K.: "A sighted man with non-24-hour sleep-wake syndrome showed damped plasma melatonin rhythm." Psychiat.Clin.Neurosci.51. 115-119 (1997)
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