RNAポリメラーゼの分子解剖:転写因子接点の同定と構造解析

• 研究課題/領域番号: 08458224
• 研究種目: 基盤研究(B)
• 配分区分: 補助金
• 応募区分: 一般
• 研究分野: 分子生物学
• 研究機関: 国立遺伝学研究所
• 研究代表者: 石浜 明 国立遺伝学研究所, 分子遺伝研究系, 教授 (80019869)
• 研究分担者: 藤田 信之 国立遺伝学研究所, 分子遺伝研究系, 助手 (90173434)
• 研究期間 (年度): 1996 – 1997
• 研究課題ステータス: 完了 (1997年度)
• 配分額: 7,400千円 (直接経費: 7,400千円); 1997年度: 2,800千円 (直接経費: 2,800千円); 1996年度: 4,600千円 (直接経費: 4,600千円)
• キーワード: 転写制御 / 転写装置 / 蛋白質間相互作用 / 蛋白質DNA相互作用 / RNAポリメラーゼ / 人工プロテアーゼ / 人工ヌクレアーゼ / 転写因子 / DNA-蛋白質相互作用 / 蛋白質-蛋白質相互作用 / 分子集合 / 構造-機能相関 / 大腸菌

研究概要

遺伝子発現制御の本質は、多種多様の転写調節蛋白因子や転写調節DNAシグナルとの相互作用によって、RNAポリメラーゼの特異性が変化し、その結果、転写対象遺伝子が変動にすることである。RNAポリメラーゼの特異性変化の機構を解明する目的で、大腸菌に約100種存在すると推定される大腸菌各種転写因子との接点を、RNAポリメラーゼ分子上に、アミノ酸配列の水準で同定することを目指した。まず、それぞれの転写因子と筆触不能となったRNAポリメラーゼ変異体を多数単離し、変異の位置を決定することから、接点を予測した。こうして得られた予測は、蛋白分子間クロスリンク法、接点特異的化学的切断法などの化学反応を利用して実証を試みた。特に、我々が最近開発した、人工プロテアーゼ活性、人工ヌクレアーゼ活性をもつFe-BABAをRNAポリメラーゼ各サブユニットに共有結合で固定し、その領域と接触する相手蛋白質またはDNAを接点で切断し、切断点をヌクレオチドまたはアミノ酸配列から同定することで、接点の直接証明する方策が成果をもたらした。この成果は、転写制御の研究に突破口をもたらしたのみならず、生命現象各分野での、分子間コミュニケーション研究に広く適用できるために、高い評価を得た。

研究成果

• [文献書誌] Buyukuslu, N.: "Physical mapping of a collection of Mael-generating amber mutations and the functional effect of internal deletions constructed through their manipulation" Genes and Function. 1(2). 119-130 (1997)
  • 説明: 「研究成果報告書概要(和文)」より
• [文献書誌] Chugani, S.A.: "Activation of the catBCA promoter:probing the interaction of CatR and RNA polymerase through in vitro transcription." J.Bacteriol.179(7). 2221-2227 (1997)
  • 説明: 「研究成果報告書概要(和文)」より
• [文献書誌] Choy, H.E.: "Repression and activation of prooter-bound RNA polymerase activity by Gal repressor." J.Mol.Biol.272(3). 293-300 (1997)
  • 説明: 「研究成果報告書概要(和文)」より
• [文献書誌] Greiner, D.P.: "Synthesis of the protein cutting reagent iron(S)-1-(p-bromoacetamide-benzym)ethylenediaminetetraacetate and conjucation to cysteine side chains." Bioconjugate Chemistry. 8(1). 44-48 (1997)
  • 説明: 「研究成果報告書概要(和文)」より
• [文献書誌] Ishihama.A.: "Adaptation of gene expression in stationary phase bacteria." Curr.Opn.Genet.Devel.7(5). 582-588 (1997)
  • 説明: 「研究成果報告書概要(和文)」より
• [文献書誌] Jeon, Y.H.: "Flexible linker in the RNA polymerase α subunit facilitates the independent motion of the C-terminal activator contact domain." J.Mol.Biol.267. 953-962 (1997)
  • 説明: 「研究成果報告書概要(和文)」より
• [文献書誌] Jishage, M.: "Variation in RNA polymerase sigma subunit composition within different stocks of Escherichia coli strain W3110." J.Bacteriol.179(3). 959-963 (1997)
  • 説明: 「研究成果報告書概要(和文)」より
• [文献書誌] Kundu, T.K: "Promoter selectivity of Escherichia coli RNA polymerase O^F holoenzyme involved in transcription of flagellar and chemotaxis genes." J.Bacteriol.179(13). 4264-4269 (1997)
  • 説明: 「研究成果報告書概要(和文)」より
• [文献書誌] Kusano, S.: "Stimulatory effect of trehalose on the formation and activity of Escherichia coli RNA polymerase EQ^<38> holoenzyme." J.Bacteriol.179(11). 3649-3654 (1997)
  • 説明: 「研究成果報告書概要(和文)」より
• [文献書誌] Kusano, S.: "Functional interaction of Escherichia coli RNA polymerase with inorganic polyphosphate." Genes to Cells. 2(7). 433-441 (1997)
  • 説明: 「研究成果報告書概要(和文)」より
• [文献書誌] McHall, S.M.: "DNase I footprinting,DNA bending and in vitro transcription analysis of CIcR and CatR on the clcABD promoter:Evidence of a conserved trascriptional activation mechanism." Mol.Microbiol.24(5). 965-976 (1997)
  • 説明: 「研究成果報告書概要(和文)」より
• [文献書誌] Iurakami, K.: "The two alpha subunits of Escherichia coli RNA polymerase are asymmetrically arranged and contact diferent halves of the DNA UP element." Proc.Natl.Acad.Sci.USA. 94(5). 1709-1714 (1997)
  • 説明: 「研究成果報告書概要(和文)」より
• [文献書誌] Murakami, K.: "Positioning of two alpha subunits carboxy-terminal domains of RNA polymera at promoters by two transcription factors." Proc.Natl.Acad.Sci.USA.94(21). 11274-11278 (1997)
  • 説明: 「研究成果報告書概要(和文)」より
• [文献書誌] Negre, D.: "DNA flexibility of the UP element is a major determinant for transcriptional activation at the Escherichia coli acetate promoter." Nucleic Acids Res.25(4). 713-718 (1997)
  • 説明: 「研究成果報告書概要(和文)」より
• [文献書誌] Ozoline, O.N.: "Specific fluorescent labeling of two functional domains in RNA polymeras alpha subunit." PROTEINS:Structure,Function,and Genetics. 30(1). 1-10 (1997)
  • 説明: 「研究成果報告書概要(和文)」より
• [文献書誌] Rajkumari, K: "Evidence for transcription attenuation rendering cryptic O^s-dependent.promoter for the osmotically regulated proU operon of Salmonella typhimun" J.Bacteriol.179(22). 7169-7173 (1997)
  • 説明: 「研究成果報告書概要(和文)」より
• [文献書誌] Yang, J: "Amino acid residues in the α subunit C-terminal domain of Escherichia coli RNA polymerase involved in interactions with an UP-like sequence and the TyrR protein in the control of transcription from the mtr promoter." J.Bacteriol.179(19). 6187-6191 (1997)
  • 説明: 「研究成果報告書概要(和文)」より
• [文献書誌] Chatterji, D.: "The mediator for stringent control,ppGpp,binds to the β-subunit of Escherichia coli RNA polymerase." Genes to Cells. in press.
  • 説明: 「研究成果報告書概要(和文)」より
• [文献書誌] Ishihama, A.: "Subunits of yeast RNA polymerases in structure and function." Curr.Opn.Microbiol.1(2). 190-196 (1998)
  • 説明: 「研究成果報告書概要(和文)」より
• [文献書誌] Jishage, M.: "A stationary phase protein in Escherichia coli with binding activity to the major Q subunit of RNA polymerase." Proc.Natl.Acad.Sci.USA. in press.
  • 説明: 「研究成果報告書概要(和文)」より
• [文献書誌] Miyake, R.: "The dimeric association of E.coli RNA polymerase alpha subunits,studied by cleavage of Fe-BABE conjugated single-cysteine alpha subunits." Biochemistry. 37(5). 1344-1349 (1998)
  • 説明: 「研究成果報告書概要(和文)」より
• [文献書誌] Owens, J.T.: "Mapping the Sigma-70 Subunit Mapping the Contact Sites on the Core Enzyme Subunits of Escherichia coli RNA polymerase by Site-Specific Cleavage with Sigma-Conjugated Chemical Protease." Proc.Natl.Acad.Sci.USA. in press.
  • 説明: 「研究成果報告書概要(和文)」より
• [文献書誌] Ozoline, O.N.: "Topology of the Escherichia coli RNA polymerase surface involved in interaction with promoter upsteam enhancer elements." Eur.J.Biochem.in press.
  • 説明: 「研究成果報告書概要(和文)」より
• [文献書誌] Ozoline, 0.N.: "Specific fluorescent labeling of two functional domains in RNA polymerase alpha subunit." PROTEINS:Structure,Function,and Genetics. 30. 183-192 (1998)
  • 説明: 「研究成果報告書概要(和文)」より
• [文献書誌] Ishihama, A.: "Nucleic Acids & Molecular Biology,Vol.11,Mechanism of Transcription" Springer-Verlag, 327 (1997)
  • 説明: 「研究成果報告書概要(和文)」より
• [文献書誌] Ishihama, A.: "Biomolecular Interactions in DNA and Proteins" Nova Sci.Publ., (1997)
  • 説明: 「研究成果報告書概要(和文)」より
• [文献書誌] Apriakawam wang, A., Fukuchi, J., Kashiwagi, K., Kakinuma, Y., Ito, E., Ishihama, A.and Igarashi, K.: "Enhancement of cell death due to the inhibition of OmpFsynthesis by RMF deficiency in the double rmf ompC mutant." J.Bacteriol.(in press).
  • 説明: 「研究成果報告書概要(欧文)」より
• [文献書誌] Ballesteros, M., Kusano, S., Ishihama, A.and Vicente, M.: "The ftsQ1p gearbox promoter of Escherichia coli is a major sigma S-dependent promoter in the ddlB-ftsA refion." Mol.Microbiol.(in press).
  • 説明: 「研究成果報告書概要(欧文)」より
• [文献書誌] Chatterji, D., Fujita, N.and Ishihama, A.: "The mediator for stringent control, ppGpp, binds to the beta-subunit of Escherichia coli RNA polymerase." Genes to Cells.(in press).
  • 説明: 「研究成果報告書概要(欧文)」より
• [文献書誌] Honda, A., Mizumoto, K.and Ishihama, A.: "A simple enzymatic method for analysis of RNA 5'-terminal structures : Quantitative determination of the 5' termini of influenza virus genome RNAs." Virus Res.(in press).
  • 説明: 「研究成果報告書概要(欧文)」より
• [文献書誌] Ishiguro, A., Kimura, M., Yasui, K., Iwata, A., Ueda, S.and Ishihama, A.: "Two large subunits of the fission yeast RNA polymerase II provide a platform for the assembly of small subunits." J.Mol.Biol.(in press).
  • 説明: 「研究成果報告書概要(欧文)」より
• [文献書誌] Ishihama, A., Kimura, M.and Mitsuzawa, H.: "Subunits of yeast RNA polymerases in structure and function." Curr.Opn.Microbiol.(in press).
  • 説明: 「研究成果報告書概要(欧文)」より
• [文献書誌] Ishihama, A., Zou, C., Kobayashi, M., Kumar, A., Fujita, N., Sakurai, H.and Hayward, R.S.: "Molecular recognition in gene transcription." In : Biomolecular Interactions in DNA and Proteins. (Buckin, V.and Funck, T., eds.). Nova Sci.Publ.(1998)
  • 説明: 「研究成果報告書概要(欧文)」より
• [文献書誌] Jishage, M.and Ishihama, A.: "A stationary phase protein in Escherichia coli with binding activity to the major sigma subunit of RNA polymerase." Proc.Natl.Acad.Sci.USA.(in press).
  • 説明: 「研究成果報告書概要(欧文)」より
• [文献書誌] McFall, S.M., Ishihama, A.and Chakrabatry, A.M.: "Transcriptional acrivation of the clcABD chlorocatechol operon : Interaction between ClcR bound to the promoter proximal site and RNA polymerase." J.Bacteriol.(in press).
  • 説明: 「研究成果報告書概要(欧文)」より
• [文献書誌] Miyake, R., Murukami, K., Owens, JT., Greiner, DP., Ozoline, O.N., Ishihama, A.and Meares, C.F.: "The dimeric association of E.coli RNA polymerase alpha subunits, studied by cleavage of Fe-BABE conjugated single-cysteine alpha subunits." Biochemistry. 37. 1344-1349 (1998)
  • 説明: 「研究成果報告書概要(欧文)」より
• [文献書誌] Miyao, T., Honda, A., Qu, Z.and Ishihama, A.: "Molecular assembly of RNA polymerase II from the fission yeast Schizosaccharomyces pombe : Subunit-subnit contact sites on Rpbl and Rpb2." Mol.Gen.Genet.(in press).
  • 説明: 「研究成果報告書概要(欧文)」より
• [文献書誌] Mukherjee, A., Cui, Y., Ma, W.L., Liu, Y., Ishihama, A., Eisenstark, A.and Chatterjee, A.K.: "RpoS (Sigma-S) controls the expression of rmsA,a global regulator of secondary metabolites, Harpin_<ECC> and extracellular proteins in Ervinia carotovora." J.Bacteriol.(in press).
  • 説明: 「研究成果報告書概要(欧文)」より
• [文献書誌] Nagai, S., Nagasawa, Y., Yagihashi, T.and Ishihama, A.: "The cyclic AMP receptor protein (CRP) gene function is required for expression of alpha (cell-bound)-type hemolysis in an avian pathogenic Escherichia coli strain." Infect.Immun.(in press).
  • 説明: 「研究成果報告書概要(欧文)」より
• [文献書誌] Negre, D., Oudot, C., Prost, J.-F., Murakami, K., Ishihama, A., Cozzone, A.J.and Cortay, J.-C.: "FruR-mediated transcriptional activation at the ppsA promoter of Escherichia coli." J.Mol.Biol.(in press).
  • 説明: 「研究成果報告書概要(欧文)」より
• [文献書誌] Nomura, T., Fujita, N.and Ishihama, A.: "Mapping of subunit-subunit contact sites on the beta subunit of Escherichia coli RNA polymerase."
  • 説明: 「研究成果報告書概要(欧文)」より
• [文献書誌] Owens, J.T., Murakami, K., Chmura, A., Fujita, N., Ishihama, A.and Meares, C.F.: "Mapping lac UV5 DNA sites proximal to conserved regions of sigma-70 in an Escherichia coli RNA polymerase-DNA open promoter complex." Biochemistry. (in press).
  • 説明: 「研究成果報告書概要(欧文)」より
• [文献書誌] Owens, J.T., Miyake, R., Murakami, K., Fujita, N., Chmura, A.J., Ishihama, A.and Meares, CF.: "Mapping the Sigma-70 Subunit Mapping the Contact Sites on the Core Enzyme Subunits of Escherichia coli RNA Poymerase by Site-Spesific Cliavage with Sigma-Conjugated Chemical Protease." Proc.Natl.Acad.Sci.USA.(in press).
  • 説明: 「研究成果報告書概要(欧文)」より
• [文献書誌] Ozoline, O.N., Fujita, N., Murakami, K.and Ishihama, A.: "Topoloty of the Escherichia coli RNA polymerase surface involved in interaction with promoter upsteam enhancer elements." Eur.J.Biochem.(in press).
  • 説明: 「研究成果報告書概要(欧文)」より
• [文献書誌] Ozoline, O.N., Murakami, K., Negishi, T., Fujita, N.and Ishihama, A.: "Specific fluorescent labeling of two functional domains in RNA polymerase alphasubunit." PROTEINS : Structure, Function, and Genetics. 30. 183-192 (1998)
  • 説明: 「研究成果報告書概要(欧文)」より
• [文献書誌] Ryu, S., Fujita, N., Ishihama, A.and Adhya, S.: "GalR-mediated repression and activation of lacUV5 promoter : Different contacts with RNA polymerase." Gene. (in press).
  • 説明: 「研究成果報告書概要(欧文)」より
• [文献書誌] Sun, W., Hattman, S., Fujita, N.and Ishihama, A.: "Acrivation of bacteriophage Mumom transcription by the C protein does not require specific interaction with the carboxyl terminal region of the alpha and sigma^<70> subunit of Escherichia coli RNA polymerase." J.Bacteriol.(in press).
  • 説明: 「研究成果報告書概要(欧文)」より
• [文献書誌] Thomas, M.S., Zou, C., Ishihama, A.and Glass, R.E.: "The effect of a nested set of C-terminal substituted deletions on the function of the alpha subunit of Escherichia coli RNA polymerase." J.Bacteriol.(in press).
  • 説明: 「研究成果報告書概要(欧文)」より
• [文献書誌] Yasui, K., ishiguro, A.and Ishihama, A.: "Molecular assembly of RNA polymerase II from the fission yeast Schizosaccharomyces pombe : Subunit-subunit contact sites on Rpb3." Biochemistry. (in press).
  • 説明: 「研究成果報告書概要(欧文)」より
• [文献書誌] Wang, P., Yang, J., Ishihama, A.and Pittard, A.J.: "Demonstration that the TyrR prorein and RNA polymerase complex formed at the divergent P3 promoter inhibits the binding of RNA polymerase to the major promoter P1 of the aroP gene of Escherichia coli." J.Bacteriol.(in press).
  • 説明: 「研究成果報告書概要(欧文)」より
• [文献書誌] Asano, Y.and Ishihama, A.: "Identification of two nucleotide-biding sites on teh PB1 subunit of influenza virus RNA polymerase." J.Biochem.122(3). 627-634 (1997)
  • 説明: 「研究成果報告書概要(欧文)」より
• [文献書誌] Boucher, P.E., Murakami, K., Ishihama, A.and Stibitz, S.: "The nature of DNA-binding and RNA polymerase interaction of the Bordetella pertusis BvgA transcriptional activator at the fha promoter." J.Bacteriol.179(5). 1755-1763 (1997)
  • 説明: 「研究成果報告書概要(欧文)」より
• [文献書誌] Buyukuslu, N., Trigwell, S., Lin, P.P., Ralphs, N.T., Fujita, N., Ishihama, A.and Glass, R.E.: "Physical mapping of a collection of MaeI-generating amber mutations and the functional effect of internal delections constructed through their manipulation." Genes and Function. 1(2). 119-130 (1997)
  • 説明: 「研究成果報告書概要(欧文)」より
• [文献書誌] Chugani, S.A., Parsek, M.R., Douglas, Hershberger, C.D., Murakami, K., Ishihama, A.and Chakrabarty, A.M.: "Activation of the catBCA promoter : Probing the interaction of CatR and RNA polymerase through in vitro transcription." J.Bacteriol.179(7). 2221-2227 (1997)
  • 説明: 「研究成果報告書概要(欧文)」より
• [文献書誌] Choy, H.E., Hanger, R.R., Aki, T., Mahoney, M., Murakami, K., Ishihama, A.and Adhya, S.: "Repression and activation of prooter-bound RNA polymerase activity by Gal repressor." J.Mol.Biol.272(3). 293-300 (1997)
  • 説明: 「研究成果報告書概要(欧文)」より
• [文献書誌] Greiner, D.P., Miyake, R., Moran, J.K., Jones, A.D., Negishi, T., Ishihama, A.and Meares, C.F.: "Synthesis of the protein cutting reagent iron (S)-1-(p-bromoacetamidebenzym) ethylenediamineteraacetate and conjucation to cysteine side chains." Bioconjugate Chemistry. 8(1). 44-48 (1997)
  • 説明: 「研究成果報告書概要(欧文)」より
• [文献書誌] Honda, A.and Ishihama, A.: "The molecular anatomy of influenza virus RNA polymerase." Biol.Chem.378. 483-488 (1997)
  • 説明: 「研究成果報告書概要(欧文)」より
• [文献書誌] Ishihama, A.: "Promoter selectivity control of Escherichia coli RNA polymerase." Nucleic Acids & Molecular Biology, Vol.11, Mechanism of Transcription, Eds.F.Eckstein and D.Lilley. Springer-Verlag, Heidelberg. 53-70 (1997)
  • 説明: 「研究成果報告書概要(欧文)」より
• [文献書誌] Ishihama, A.: "Adaptation of gene expression in stationary phase bacteria." Curr.Opn.Genet.Devel.7(5). 582-588 (1997)
  • 説明: 「研究成果報告書概要(欧文)」より
• [文献書誌] Ishihama, A., Zou, C., Kobayashi, M., Kumar, A., Fujita, N., Sakurai, H.and Hayward, R.S.: "Molecular recognition in gene transcription." In : Biomolecular Interactions in DNA and Proteins.(Buckin, V.and Funck, T., eds.). Nova Sci.Publ.(1996)
  • 説明: 「研究成果報告書概要(欧文)」より
• [文献書誌] Jeon, Y.H., Yamazaki, T., Otomo, T., Ishihama, A.and Kyogoku, Y.: "Flexible linker in the RNA polymerase alpha subunit facilitates the independent motion of the C-terminal activator contact domain." J.Mol.Bilo.267. 953-962 (1997)
  • 説明: 「研究成果報告書概要(欧文)」より
• [文献書誌] Jishage, M.and Ishihama, A.: "Variation in RNA prolymerase sigma subunit composition within different stocks of Escherichia coli strain W3110." J.Bacteriol.179(3). 959-963 (1997)
  • 説明: 「研究成果報告書概要(欧文)」より
• [文献書誌] Kang, J.G., Hahn, M.Y., Ishihama, A.and Roe, J.H.: "Identification of sigma factors for growth phase-related promoter selectivity of RNA polymerases from Steptomyces coelicolor A3(2)." Nucleic Acids Res.25. 2566-2573 (1997)
  • 説明: 「研究成果報告書概要(欧文)」より
• [文献書誌] Kimura, M., Ishiguro, A.and Ishihama, A.: "RNA polymerase II subunits 2,3 and 11 form a core subassembly with DNA binding activity." J.Biol.Chem.272. 25851-25855 (1997)
  • 説明: 「研究成果報告書概要(欧文)」より
• [文献書誌] Kundu, T.K., Kusano, S.and Ishihama, A.: "Promoter selectivity of Escherichia coli RNA polymerase sigma^F holonenzyme involved in transcription of flagellar and chemotaxis genes." J.Bacteriol.179(13). 4264-4269 (1997)
  • 説明: 「研究成果報告書概要(欧文)」より
• [文献書誌] Kusano, S.and Ishihama, A.: "Stimulatory effect of trehalose on the formation and activity of Escherichia coli RNA polymerase Esigma^<38> holoenzyme." J.Bacteriol.179(11). 3649-3654 (1997)
  • 説明: 「研究成果報告書概要(欧文)」より
• [文献書誌] Kusano, S.and Ishihama, A.: "Functional ionteraction of Escherichia coli RNA polymerase with inorganic polyphosphate." Genes to Cells. 2(7). 433-441 (1997)
  • 説明: 「研究成果報告書概要(欧文)」より
• [文献書誌] McHall, S.M., Klem, T.J., Fujita, N., Ishihama, A.and Chakrabarty, A.M.: "DNase I footprinting, DNA bending and in vitro transcription analysis of ClcR and CatR on the clcABD promoter : Evidence of a conserved transcriptional activation mechanism." Mol.Microbiol.24. 965-976 (1997)
  • 説明: 「研究成果報告書概要(欧文)」より
• [文献書誌] Murakami, K., Kimura, M., Owens, J.T., Meares, C.F.and Ishihama, A.: "The two alpha subunits of Escherichia coli RNA polymerase are asymmetrically arranged and contact diferent halves of the DNA UP element." Proc.Natl.Acad.Sci.USA. 94(5). 1709-1714 (1997)
  • 説明: 「研究成果報告書概要(欧文)」より
• [文献書誌] Murakami, K., Owens, J.T., Belaeva, T.A., Meares, C.F., Busby, S.J.W.and Ishihama, A.: "Positioning of two alpha subunits carboxy-terminal domains of RNA polymerase at promoters by two transcription factors." Proc.Natl.Acad.Sci.UISA. 94(21). 11274-11278 (1997)
  • 説明: 「研究成果報告書概要(欧文)」より
• [文献書誌] Negre, D., Bonod-Bidaud, C., Oudor, C., Prost, J.-F., Kolb, A., Ishihama, A., Cozzone, A.J.and Cortay, J.-C.: "DNA flexbility of the UP element is a major determinant for transcriptional activation at the Escherichia coli acetate promoter." Nucleic Acids Res.25(4). 713-718 (1997)
  • 説明: 「研究成果報告書概要(欧文)」より
• [文献書誌] Ozoline, O.N., Masulisi, I.S., Chasovi, V.V.and Ishihama, A.: "Functional topology of the bacterial RNA polymerase." In : Spectroscopy of Biological Molecules : Modern Trends. P.Carmona, R.Navarro and A.Hernanz (eds.). Kluwer Acadenmic Publ., Dordrecht. 163-164 (1997)
  • 説明: 「研究成果報告書概要(欧文)」より
• [文献書誌] Rajkumari, K., Ishihama, A.and Gowrishankar, J.: "Evidence for transcription attenuation rendering crypric sigma^S-dependent promoter for the osmotically regulated oroU operon of Salmonella typhimurium." J.Bacteriol.179. 7169-7173 (1997)
  • 説明: 「研究成果報告書概要(欧文)」より
• [文献書誌] Sakurai, H.and Ishihama, A.: "Gene organization and sequence of the small subunits of Schizosaccharomyces pombe RNA polymerase II." Gene. 196. 165-174 (1997)
  • 説明: 「研究成果報告書概要(欧文)」より
• [文献書誌] Yang, J., Murakami, K., Camakaris, H., Fujita, N., Ishihama, A.and Pittard, A.J.: "Amino acid residues in the alpha subunit C-terminal domain of Escherichia coli RNA polymerase involed in interactions with and UP-like sequence and the TryR protein in the control of transcription from the mtr promoter." J.Bacteriol.179(19). 6187-6191 (1997)
  • 説明: 「研究成果報告書概要(欧文)」より
• [文献書誌] Artsimovitch, I., Murakami, K., Ishihara, A.and Howe, M.M.: "Transcription activation by the bacteriophage Mu Mor protein requires the C-terminal regions of both alpha and sigma^<70> subunists of Escherichia coli RNA polymerase." J.Biol.Chem.271. 32343-32348 (1996)
  • 説明: 「研究成果報告書概要(欧文)」より
• [文献書誌] Belyaeva, T., Bown, J., Fujita, N., Ishihama, A.and Busby, S.: "Location of the C-terminal domain of the RNA polymerase alpha subunit in different open complexes at the Escherichia coli galactose operon regulatory region." Nucleic Acids Res.24. 2243-2251 (1996)
  • 説明: 「研究成果報告書概要(欧文)」より
• [文献書誌] Eichenberger, P., Dethiollaz, S., Fujita, N., Ishihama, A.and Geiselmann, J.: "The influence of the location of the activator binding site on the geometry of a transcriptional activation.complex in Escherichia coli." Bichemistry. 35. 15302-15312 (1996)
  • 説明: 「研究成果報告書概要(欧文)」より
• [文献書誌] Fujita, N., and Ishihama, A.: "Reconstitution of RNA polymerase." Meth.Enzymol. 273, RNA Polymerase and Associated Factors, Part A,Adhya, S., ed.121-130 (1996)
  • 説明: 「研究成果報告書概要(欧文)」より
• [文献書誌] Gilade, H., Murakami, K., Ishihama, A.and Oppenheim, A.B.: "Identification of an UP element within the IHF binding site at the P_L1-P_L2 tandem promoter of bacteriophage lambda." J.Mol.Biol. 260. 484-491 (1996)
  • 説明: 「研究成果報告書概要(欧文)」より
• [文献書誌] Ishihama, A.: "A multi-functional enzyme with RNA polymerase and RNase acrivities : Molecular anatomy of influenza virus RNA polymerase." Biochimie. 78. 1097-1102 (1996)
  • 説明: 「研究成果報告書概要(欧文)」より
• [文献書誌] Imamura, R., Yamanaka, K., Ogura, T., Hiraga, S., Fujita, N., Ishihama, A.and Niki, H.: "Identification of the cdpA gene encoding cyclic 3', 5'-adenosine phosphophate phosphodiesterse in Escherchia coli." J.Biol.Chem.271. 25423-25429 (1996)
  • 説明: 「研究成果報告書概要(欧文)」より
• [文献書誌] Ishihama, A., Asano, Y., Honda, A., Toyoda, T., Mizumoto, K.and Nakada, S.: "Structure, function and formation of influenza virus RNA polymerase." In : Options for the Control of Influenza, Eds.L.E.Brown, A.W.Hampson and R.G.Webster. Elsevier Sci.B.V., Amsterdam. 372-380
  • 説明: 「研究成果報告書概要(欧文)」より
• [文献書誌] Jair, K.-W., Fawcett, W.P., Fujita, N., Ishihama, A.and Wolf, Jr., R.E.: "Ambidextrous transcriprion activation by SoxS : Requirement for the C-terminal domain of the RNA polymerase alpha subunit at a subset of superoxide inducible genes." Mol.Microbiol.19. 307-314 (1996)
  • 説明: 「研究成果報告書概要(欧文)」より
• [文献書誌] Jair, K.-W., Yu, X., Skarstad, K., Thony, B., Fujita, N., Ishihama A.and Wolf, Jr., R.E.: "Transcription activation of promoters of the superoxide and multiple antibiotic resistance regulation by Rob, a binding protein of the Escherichia coli origin of chromosomal replication." J.Bacteriol.178. 2507-2513 (1996)
  • 説明: 「研究成果報告書概要(欧文)」より
• [文献書誌] Jishage, M., Iwata, A., Ueda, S.and Ishihama, A.: "Regulation of RNA polymerase sigma subunit synthesis in Escherichia coli : Intracellular levels of four speoies of sigma subunit under various growth conditions." J.Bacteriol.178. 5447-5451 (1996)
  • 説明: 「研究成果報告書概要(欧文)」より
• [文献書誌] Kawagishi : Kobayashi, M., Yamamoto, M.and Ishihama, A.: "Mutational analysis of the RNase-like domain in subunit 2 of fission yeast RNA polymerase II." Mol.Gen.Genet.205. 1-5 (1996)
  • 説明: 「研究成果報告書概要(欧文)」より
• [文献書誌] Kmura, M.and Ishihama, A.: "Subunit assembly in vivo of Escherichia coli RNA polymerase : Role of the amino-terminal assembly domain of alpha subunit" Genes To Cells. 1. 517-528 (1996)
  • 説明: 「研究成果報告書概要(欧文)」より
• [文献書誌] Kobayashi, M., Toyoda, T.and Ishihama, A.: "Influenza virus PB1 protein is the minimal and essential subunit of RNA polymerase." Arch.Viorl.141. 525-533 (1996)
  • 説明: 「研究成果報告書概要(欧文)」より
• [文献書誌] Kusano, S., Ding, Q., Fujita, N.and Ishihama, A.: "Promoter selectivity of Escherichia coli RNA Polymerase Esigma^<70> and Esigma^<38> holoenzymes : Effect of DNA supercoiling." J.Biol.Chem.271. 1998-2004 (1996)
  • 説明: 「研究成果報告書概要(欧文)」より
• [文献書誌] Luo, J., Sharif, K.A., Jin, R., Fujita, N., Ishihama, A.and Krakow, J.S.: "Molecular anatomy of the beta' subunit of the E.coli RNA polymerase : Identification of regions involved in polymerase assembly." Genes to Cells. 1. 819-828 (1996)
  • 説明: 「研究成果報告書概要(欧文)」より
• [文献書誌] Miyao, T., Yasui, K., Sakurai, H., Yamagishi, M.and Ishihama, A.: "Molecular assembly of RNA polymerase II from the fission yeast Schizosacharomyces pombe : Subunit-subunit contact network involving Rpb5." Genes to Cells. 1(9). 843-855 (1996)
  • 説明: 「研究成果報告書概要(欧文)」より
• [文献書誌] Murakami, K., Fujita, N.and Ishihama, A.: "Transcription factor recognition surface on the RNA polymerase alpha subunit is involved in contact with the DNA enhancer element." EMBO J.15. 4358-4367 (1996)
  • 説明: 「研究成果報告書概要(欧文)」より
• [文献書誌] Rajkumari, K., Kusano, S., Ishihama, A., T.Mizuno and Gowrishankar, J.: "Effects of H-NS and potassium glutamate on sigma^S-andsigma^<70>-directed transcription in vitro from osmotically regulated P1 and P2 promoters of proU in Eschercihia coli." J.Bacteriol.178. 4176-4181 (1996)
  • 説明: 「研究成果報告書概要(欧文)」より
• [文献書誌] Sakurai, H., Miyao, T.and Ishihama, A.: "Subunit composition of RNA polymerase II from the fission yeast Schizosaccharomyces pombe." Gene. 180. 63-67 (1996)
  • 説明: 「研究成果報告書概要(欧文)」より
• [文献書誌] Tang, Y., Murakami, K., Ishihama, A.and deHaseth, P.L.: "Upstream interactions at the lambda P_<RM> promoter are sequence-nonspecific and activate the promoter to a lesser extent than an introduced UP element of an rRNA promoter." J.Bacteriol.178. 6945-6951 (1996)
  • 説明: 「研究成果報告書概要(欧文)」より
• [文献書誌] Toyoda, T., Adyshev.DM., Kobayashi, M., Iwata, A.and Ishihama, A.: "Molecular assembly of influenza virus RNA polymerase : Identification of the subunit-subunit contact sites on each P protein subunit." J.Gen.Virol.77. 2149-2157 (1996)
  • 説明: 「研究成果報告書概要(欧文)」より
• [文献書誌] Toyoda, T., Adyshex.DM., Kobayashi, M., Iwata, A.and Ueda, S.and Ishihama, A.: "The subunit-subunit contact sites of the influenza virus RNA polymerase." Oprions for Control of Influenza, Eds.L.E.Brown, A.W.Hampson and R.G.Webster. Elsevier Sci.B.V., Amsterdam.419-426 (1996)
  • 説明: 「研究成果報告書概要(欧文)」より
• [文献書誌] Toyoda, T., Kobayashi, M., Nakada, S and Ishihama, A.: "Molecular dissection of influenza virus RNA polymerase : PB1 subunit alone is able to catalyze RNA synthesis." Virus Genes. 12. 155-161 (1996)
  • 説明: 「研究成果報告書概要(欧文)」より
• [文献書誌] Buyukuslu,N.: "Physical mapping of a collection of Mael-generating amber mutations and the functional effect of internal deletions constructed through their manipulation." Genes and Function. 1(2). 119-130 (1997)
• [文献書誌] Chugani,S.A.: "Activation of the catBCA promoter: Probing the interaction of CatR and RNA polymerase through in vitro transcription." J.Bacteriol.179(7). 2221-2227 (1997)
• [文献書誌] Choy,H.E.: "Repression and activation of prooter-bound RNA polymerase activity by Gal repressor." J.Mol.Biol.272(3). 293-300 (1997)
• [文献書誌] Greiner,D.P.: "Synthesis of the protein cutting reagent iron (S)-1-(p-bromoacetamide-benzym) ethylenediaminetetraacetate and conjucation to cysteine side chains." Bioconjugate Chemistry. 8(1). 44-48 (1997)
• [文献書誌] Ishihama,A.: "Adaptation of gene expression in stationary phase bacteria." Curr.Opn.Genet.Devel.7(5). 582-588 (1997)
• [文献書誌] Jeon,Y.H.: "Flexible linker in the RNA polymerase α subunit facilitates the independent motion of the C-terminal activator contact domain." J.Mol.Biol.267. 953-962 (1997)
• [文献書誌] Murakami,K.: "Positioning of two alpha subunits carboxy-terminal domains of RNA polymerase at promoters by two transcription factors." Proc.Natl.Acad.Sci.USA. 94(21). 11274-11278 (1997)
• [文献書誌] Negre,D.: "DNA flexibility of the UP element is a major determinant for transcriptional activation at the Escherichia coli acetate promoter." Nucleic Acids Res.25(4). 713-718 (1997)
• [文献書誌] Ozoline,O.N.: "Specific fluorescent labeling of two functional domains in RNA polymerase alpha subunit." PROTEINS:Structure,Function,and Genetics. 30(1). 1-10 (1997)
• [文献書誌] Rajkumari,K.: "Evidence for transcription attenuation redering cryptic σ^S-dependent promoter for the osmotically regulated proU operon of Salmonella typhimurium" J.Bacteriol.179(22). 7169-7173 (1997)
• [文献書誌] Yang,J.: "Amino acid residues in the α subunit C-terminal domain of Escherichia coli RNA polymerase involved in interactions with an UP-like sequence and the TyrR protein in the control of transcription from the mtr promoter." J.Bacteriol.179(19). 6187-6191 (1997)
• [文献書誌] Chatterji,D.: "The mediator for stringent control,ppGpp,binds to the β-subunit of Escherichia coli RNA polymerase." Genes to Cells. (in press).
• [文献書誌] Jishage,M.: "Variation in RNA polymerase sigma subunit composition within different stocks of Escherichia coli strain W3110." J.Bacteriol.179(3). 959-963 (1997)
• [文献書誌] Kundu,T.K.: "Promoter selectivity of Escherichia coli RNA polymerase σ^F holoenzyme involved in transcription of flagellar and chemotaxis genes." J.Bacteriol.179(13). 4264-4269 (1997)
• [文献書誌] Kusano,S.: "Stimulatory effect of trehalose on the formation and activity of Escherichia coli RNA polymerase Eσ^<38> holoenzyme." J.Bacteriol.179(11). 3649-3654 (1997)
• [文献書誌] Kusano,S.: "Functional interaction of Escherichia coli RNA polymerase with inorganic polyphosphate." Genes to Cells. 2(7). 433-441 (1997)
• [文献書誌] McHall,S.M.: "DNase I footprinting,DNA bending and in vitro transcription analysis of ClcR and CatR on the clcABD promoter: Evidence of a conserved transcriptional activation mechanism." Mol.Microbiol.24(5). 965-976 (1997)
• [文献書誌] Murakami,K.: "The two alpha subunits of Escherichia coli RNA polymerase are asymmetrically arranged and contact diferent halves of the DNA UP element." Proc.Natl.Acad.Sci.USA. 94(5). 1709-1714 (1997)
• [文献書誌] Ishihama,A.: "Subunits of yeast RNA polymerases in structure and function." Curr.Opn.Microbiol.1(2). 190-196 (1998)
• [文献書誌] Jishage,M.: "A stationary phase protein in Escherichia coli with binding activity to the major σ subunit of RNA polymerase." Proc.Natl.Acad.Sci.USA. (in press).
• [文献書誌] Miyake,R.: "The dimeric association of E.coli RNA polymerase alpha subunits,studied by cleavage of Fe-BABE conjugated single-cysteine alpha subunits." Biochemistry. 37(5). 1344-1349 (1998)
• [文献書誌] Owens,J.T.: "Mapping the Sigma-70 Subunit Mapping the Contact Sites on the Core Enzyme Subunits of Escherichia coli RNA Polymerase by Site-Specific Cleavage with Sigma-Conjugated Chemical Protease:" Proc.Natl.Acad.Sci.USA. (in press).
• [文献書誌] Ozoline,O.N.: "Topology of the Escherichia coli RNA polymerase surface involved in interaction with promoter upsteam enhancer elements." Eur.J.Biochem.(in press).
• [文献書誌] Ozoline,O.N.: "Specific fluorescent labeling of two functional domains in RNA polymerase alpha subunit." PROTEINS: Structure,Function,and Genetics. 30. 183-192 (1998)
• [文献書誌] Ishihama,A.: "Nucleic Acids & Molecular Biology,Vol.11,Mechanism of Transcription" Springer-Verlag, 327 (1997)
• [文献書誌] Ishihama,A.: "BiomolecularInteractions in DNA and Proteins" Nova Sci.Publ., (1997)
• [文献書誌] Artsimovitch,I.: "Transcription activation by the bacteriophage Mu Mor protein requires the C-terminal regions of both α and σ^<70> subunits of Escherichia coli RNA polymerase." J.Biol.Chem.271. 32343-32348 (1996)
• [文献書誌] Belyaeva,T.: "Location of the C-terminal domain of the RNA polymerase alpha subunit in diggerent open complexes at the Escherichia coli galactose operon regulatory region." Nucleic Acids Res.24・12. 2243-2251 (1996)
• [文献書誌] Eichenberger,P.: "The influence of the location of the activator binding site on the geometry of a transcriptional activation the geometry of a transcriptional activation complex in Eschericia coli." Biochemistry. 35・48. 15302-15312 (1996)
• [文献書誌] Giladi,H.: "Identification of an UP element within the IHF binding site at the P_L1-P_L2 tandem promoter of bacteriophase λ." J.Mol.Biol.260. 484-491 (1996)
• [文献書誌] Imamura,R.: "Identification of the cdpA gene encoding cyclic 3',5'-adenosine phosophate phosphodiesterase in Escherichia coli." J.Biol.Chem.271・41. 25423-25429 (1996)
• [文献書誌] Jair,K.-W.: "Ambidextrous transcription activation by Soxs : Requirement for the C-terminal domain of the RNA polymerase alpha subunit at a subset of superoxide inducible genes." Mol.Microbiol.19・2. 307-314 (1996)
• [文献書誌] Jair,K.-W.: "Transcription activation of promoters of the superoxide and multiple antibiotic resistance regulation by Rob,a binding protein of the Escherichia coli origin of chromosomal replication." J.Bacteriol.178・9. 2507-2513 (1996)
• [文献書誌] Jishage.M.: "Regulation of RNA polymerase sigma subunit synthesis in Escherichia coli : Intracellular levels of four species of sigma subunit under various growth conditons." J.Bacteriol.178・18. 5447-5451 (1996)
• [文献書誌] Kimura,M.: "Subunit assembly in vivo of Escherichia coli RNA polymerase : Role of the amino-terminal assembly domain of alpha subunit." Genes to Cells. 1・6. 517-528 (1996)
• [文献書誌] Kusano,S.: "Promoter selectivity of Escherichia coli RNA polymerase Eσ^<70> and Eσ^<38> holoenzymes : Effect of DNA supercoiling." J.Biol.Chem.271・4. 1998-2004 (1996)
• [文献書誌] Luo,J.: "Molecular anatomy of the β' subunit of the E.coli RNA polymerase : Identification of region involved in polymerase assemblymerase : Identification of regions involved in polymerase assembly" Genes to Cells. 1・9. 819-828 (1996)
• [文献書誌] Murakami,K.: "Transcription factor recognition surface on the RNA polymerase α subunit is involved in contact with the DNA enhancer element." EMBOJ.15・16. 4358-4367 (1996)
• [文献書誌] Rajkumari,K.: "Effects of H-HS and potassium glutamate on σ^<S-> and σ^<70>-directed transcription in vitro from osmotically regulated P1 and P2 promoters of proU in Escherichia coli." J.Bacteriol.178・14. 4176-4181 (1996)
• [文献書誌] Tang,Y.: "Upstream interactions at the lambda P_<RM> promoter are sequence-nonspecific and activate the promoter to a lesser extent than an introduced UP element of an rRna promoter." J.Bacteriol.178・23. 6945-6951 (1996)
• [文献書誌] Greiner,D.P.: "Synthesis of the protein cutting reagent iron (S)-1-(p-bromoacetamidebenzym) ethylenediam inetetraacetate and conjucation to cysteine side chains." Bioconjugate Chemistry. 8・1. 44-48 (1997)
• [文献書誌] Jishage,M.: "Variation in RNA polymerase sigma subunit composition within different stocks Escherichia coli strain W3110." J.Bacteriol.179・3. 959-963 (1997)
• [文献書誌] Kusano,S.: "Promoter selectivity control of Escherichia coli RNA polyerase Eσ^<38> holoenzyme by trehalose." J.Bacteriol. (in press).
• [文献書誌] Murakami,K.: "The two alpha subunits of Escherichia coli RNA polymerase are asymmetrically arranged and contact different halves of the DNA UP element." Proc.Nat.Acad.Sci.(in press).
• [文献書誌] Negre,D.: "DNA flexibility of the UP element is a major determinant for transcriptional activation at the Escherichia coli acetate promoter." Nucleic Acids Res.25・4. 713-718 (1997)