| 配分額 *注記 |
3,000千円 (直接経費: 3,000千円)
2015年度: 1,000千円 (直接経費: 1,000千円)
2014年度: 1,000千円 (直接経費: 1,000千円)
2013年度: 1,000千円 (直接経費: 1,000千円)
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| 研究実績の概要 |
I have been screening for the key transcription factors that determine pericycle cell Identity. My screening has revealed two clades of basic Helix-Loop-Helix (bHLH) family transcription factors, represented by No.17 (including No.17, No.17H, No.17H2, and No.17H3) and No. 51 (including No.51, No. 36, No.14, No.14L etc.), that play key roles in regulation pericycle cell identity. A Yeast-Two-Hybrid screening confirmed the interaction between the two clades of proteins.
No.51 is expressed specifically in pericycle cells. No.51 overexpression (No.51-OE) causes ectopic expression of XPP-marker GFP. Importantly, No.51-OE caused ectopic cell division in the root. Exogenous auxin increased the ectopic cell division in No.51-OE root, demonstrating that No.51 confers the competency to undergo auxin-induced cell division. No.17-OE caused similar but milder phenotype than that of No.51. No.17 is broadly expressed in several cell types including pericycle.
No.51 regulate No.17's transcription, but not vice versa. No.51 is also auto-regulated. Such feedback reinforces their own transcription. No.51’s specific expression pattern confines this network in the pericycle, which constitutes a master regulation of pericycle cell’s identity.
Transcriptional profiling of No.51-OE root shows that many pericycle-specific genes are up-regulated by No.51-OE, suggesting No.51’s role as a positive regulator of pericycle cell identity. Detailed analysis on the obtained transcriptional profile of No.51-OE/ No.51-SRDX roots will shed light on the identification of the bHLH TFs’ downstream effectors.
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