| 研究実績の概要 |
I designed and optimized the synthetic SINEUPs for reprogramming human iPS cells (hiPSCs) into neuron cells and tested their activity in HEK293T/17 cells. Since lipid-mediated plasmid delivery has low efficiency in hiPSCs compared to viral delivery methods, I constructed four lentiviral vectors to express transcription factor specific (NGN2, NGN3, TLX3, PAX6) SINEUPs to enhance translation of endogenous TF mRNA in hiPSCs in order to reprogram them into neurons. I transduced hiPSCs with one SINEUP or a coctail of two, three, or four diferent SINEUPs. There were clear differences in terms of neuron differentiation. Coctails of at least two different SINEUPs have a potential for hiPSC reprogramming into neurons.
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