ベクターコントロールによる有機塩素系農薬汚染が家畜・ヒトに及ぼす毒性影響の解明

• 研究課題/領域番号: 15J02013
• 研究種目: 特別研究員奨励費
• 配分区分: 補助金
• 応募区分: 国内
• 研究分野: 環境農学(含ランドスケープ科学)
• 研究機関: 北海道大学
• 研究代表者: Thompson Lesa (2017) 北海道大学, 獣医学研究科, 特別研究員(DC1); THOMPSON LESA (2015-2016) 北海道大学, 獣医学研究科, 特別研究員(DC1)
• 研究期間 (年度): 2015-04-24 – 2018-03-31
• 研究課題ステータス: 完了 (2017年度)
• 配分額: 3,400千円 (直接経費: 3,400千円); 2017年度: 1,100千円 (直接経費: 1,100千円); 2016年度: 1,100千円 (直接経費: 1,100千円); 2015年度: 1,200千円 (直接経費: 1,200千円)
• キーワード: DDT / Chicken / Transcriptomics / Metabolomics / MCF-7 cell culture / Free-range chicken / Human health risk / Microarray / KwaZulu-Natal / Free-ranging chickens / Indoor residual spraying / South Africa

研究実績の概要

Two manuscripts have been published. Various alterations in mRNA expression were detected in chickens exposed to DDTs in the field, including down-regulation of several avian beta-defensins. A manuscript on these findings is currently at the revision stage of publication.mRNA expression was assessed using qRT-PCR after in vitro exposure of p,p’-DDT in MCF-7 human breast cancer cells. Several genes relating to oxidative stress markers, xenobiotic metabolizing enzymes and transporters were significantly up-regulated after exposure. A manuscript has been submitted for peer-review.Samples from in vivo exposure of chickens to p,p’-DDT underwent further analysis. Liver histopathology did not show significant changes. qRT-PCR of liver samples confirmed altered gene expression in avian beta defensin (AvBD) genes, AvBD1 and AvBD7. Further, metabolomics analysis using hydrophilic interaction liquid chromatography (HILIC) revealed many significantly elevated metabolites relating to amino acid metabolism in exposed groups. These results are currently being prepared for journal submission.

現在までの達成度 (段落)

29年度が最終年度であるため、記入しない。

今後の研究の推進方策

29年度が最終年度であるため、記入しない。

研究成果

• [国際共同研究] North West University/BioAssets Consultants/University of Johannesburg(South Africa)
• [国際共同研究] Zagazig University(Egypt)
• [国際共同研究] University of Gondar(Ethiopia)
• [国際共同研究] University of Edinburgh/University of Glasgow/Chester Zoo(英国)
• [国際共同研究] University of Johannesburg/North West University/BioAssets Consultants(South Africa)
• [国際共同研究] Zagazig University(Egypt)
• [国際共同研究] University of Gondar(Ethiopia)
• [国際共同研究] University of Johannesburg/North West University/BioAssets Consultants(South Africa)
• [国際共同研究] Zagazig University(Egypt)
• [雑誌論文] Human Health Risk from Consumption of Marine Fish Contaminated with DDT and Its Metabolites in Maputo Bay, Mozambique (2018)
  • 著者名/発表者名: Thompson L. A.、Ikenaka Y.、Yohannes Y. B.、Ichise T.、Ito G.、Bortey-Sam N.、van Vuren J. J.、Wepener V.、Smit N. J.、Darwish W. S.、Nakayama S. M. M.、Mizukawa H.、Ishizuka M.
  • 雑誌名: Bulletin of Environmental Contamination and Toxicology 巻: 100 号: 5 ページ: 672-676
  • DOI: 10.1007/s00128-018-2323-7
  • 査読あり / オープンアクセス / 国際共著
• [雑誌論文] Concentrations and human health risk assessment of DDT and its metabolites in free-range and commercial chicken products from KwaZulu-Natal, South Africa (2017)
  • 著者名/発表者名: Thompson L. A.、Ikenaka Y.、Yohannes Y. B.、van Vuren J. J.、Wepener V.、Smit N. J.、Darwish W. S.、Nakayama S. M. M.、Mizukawa H.、Ishizuka M.
  • 雑誌名: Food Additives and Contaminants: Part A 巻: 34 号: 11 ページ: 1959-1969
  • DOI: 10.1080/19440049.2017.1357209
  • 査読あり / オープンアクセス / 国際共著
• [雑誌論文] β-carotene and retinol reduce benzo[a]pyrene-induced mutagenicity and oxidative stress via transcriptional modulation of xenobiotic metabolizing enzymes in human HepG2 cell line (2017)
  • 著者名/発表者名: Darwish Wageh Sobhy、Ikenaka Yoshinori、Nakayama Shouta、Mizukawa Hazuki、Thompson Lesa A、Ishizuka Mayumi
  • 雑誌名: Environmental Science and Pollution Research 巻: 25 号: 7 ページ: 6320-6328
  • DOI: 10.1007/s11356-017-0977-z
  • 査読あり / オープンアクセス / 国際共著
• [雑誌論文] Organochlorine pesticide contamination of foods in Africa: incidence and public health significance (2017)
  • 著者名/発表者名: L. A. Thompson, W. S. Darwish, Y. Ikenaka, S. M. Nakayama, H. Mizukawa and M. Ishizuka
  • 雑誌名: Ｔｈｅ Ｊｏｕｒｎａｌ ｏｆ Ｖｅｔｅｒｉｎａｒｙ Ｍｅｄｉｃａｌ Ｓｃｉｅｎｃｅ 巻: 79 号: 4 ページ: 751-764
  • DOI: 10.1292/jvms.16-0214
  • NAID: 130005568160
  • ISSN: 0916-7250, 1347-7439
  • 査読あり / オープンアクセス / 国際共著
• [雑誌論文] Vector-control pesticide DDT detected in free-ranging chickens from South Africa (2015)
  • 著者名/発表者名: Lesa A Thompson, Yoshinori Ikenaka, Yared B Yohannes, Johan van Vuren, Victor Wepener, Nico J Smit, Wynand Vlok, Shouta M M Nakayama, Hazuki Mizukawa and Mayumi Ishizuka
  • 雑誌名: Proceedings: 7th International Toxicology Symposium in Africa 巻: 7 ページ: 4-5
  • 査読あり / オープンアクセス / 国際共著 / 謝辞記載あり
• [学会発表] First, do no harm: balancing the benefits of malaria control against the toxic effects of DDT use (2017)
  • 著者名/発表者名: Thompson, L.A., Ikenaka, Y., Yohannes, Y., Assefa, A., van Vuren, J., Wepener, V., Smit, N., Vlok, W., Darwish, W.S., Nakayama, S., Mizukawa, H., Ishizuka, M.
  • 学会等名: 5th Sapporo Summer Seminar for One Health
  • 国際学会
• [学会発表] Comparison of food products contaminated by DDTs in South Africa and Mozambique (2017)
  • 著者名/発表者名: Thompson, L.A., Ikenaka, Y., Wepener, V., Ishizuka, M.
  • 学会等名: 19th International Conference on One Health
  • 国際学会
• [学会発表] Effect of DDTs on gallinacins in the avian immune system. (2017)
  • 著者名/発表者名: Thompson, L.A., Ikenaka, Y., Yohannes, Y., van Vuren, J., Wepener, V., Smit, N., Assefa, A., Darwish, W.S., Nakayama, S., Mizukawa, H., Ishizuka, M.
  • 学会等名: The 10th International Meeting of the Asian Society of Conservation Medicine One Health in Asia Pacific
  • 国際学会
• [学会発表] Genetic effects of the pesticide DDT on birds: lessons learned from a field study in South Africa. (2016)
  • 著者名/発表者名: Thompson, L.A., Ikenaka, Y., Yohannes, Y., Tsubota, T., van Vuren, J., Wepener, V., Smit, N., Nakayama, S., Mizukawa, H., Ishizuka, M.
  • 学会等名: The 9th International Meeting of the Asian Society of Conservation Medicine One Health in Asia Pacific
  • 発表場所: Taipei Zoo, Taiwan
  • 年月日: 2016-10-21
  • 国際学会
• [学会発表] Playing “chicken” - a xenobiotic dilemma. (2016)
  • 著者名/発表者名: Thompson, L.A., Ikenaka, Y., Yohannes, Y., Assefa, A., van Vuren, J., Wepener, V., Smit, N., Vlok, W., Nakayama, S.M.M., Mizukawa, H., Ishizuka, M.
  • 学会等名: 4th Sapporo Summer Seminar for One Health
  • 発表場所: Hokkaido University, Sapporo, Hokkaido, Japan
  • 年月日: 2016-09-20
  • 国際学会
• [学会発表] Contamination of eggs from free-ranging chickens in a malaria-endemic region using DDT as vector control. (2016)
  • 著者名/発表者名: Thompson, L.A., Ikenaka, Y., Yohannes, Y.B., van Vuren, J.J., Wepener, V., Smit, N.J., Vlok, W., Nakayama, S.M.M., Mizukawa, H., Ishizuka, M.
  • 学会等名: ISEE-ISES Asia Chapter
  • 発表場所: Hokkaido University, Sapporo, Hokkaido, Japan
  • 年月日: 2016-06-27
  • 国際学会
• [学会発表] Risks versus benefits of controlling vector-borne diseases using insecticides: a case example in South Africa. (2016)
  • 著者名/発表者名: Thompson, L.A., Ikenaka, Y., Yohannes, Y.B., van Vuren, J.J., Wepener, V., Smit, N.J., Vlok, W., Nakayama, S.M.M., Mizukawa, H., Ishizuka, M.
  • 学会等名: 2nd International Conference on One Medicine One Science
  • 発表場所: The Commons Hotel, Minneapolis, USA
  • 年月日: 2016-04-24
  • 国際学会
• [学会発表] Vector-contol pesticide DDT detected in free-ranging chickens from South Africa (2015)
  • 著者名/発表者名: Lesa A THOMPSON, Yoshinori IKENAKA, Yared B YOHANNES, Johan J VAN VUREN, Victor WEPENER, Nico J SMIT, Wynand VLOK, Shouta M M NAKAYAMA, Hazuki MIZUKAWA, Mayumi ISHIZUKA
  • 学会等名: 3rd Sapporo Summer Seminar for One Health
  • 発表場所: Hokkaido University, Sapporo, Hokkaido, Japan
  • 年月日: 2015-09-16
  • 国際学会
• [学会発表] Vector-control pesticide DDT detected in free-ranging chickens from South Africa (2015)
  • 著者名/発表者名: Lesa A Thompson, Yoshinori Ikenaka, Yared B Yohannes, Johan van Vuren, Victor Wepener, Nico J Smit, Wynand Vlok, Shouta M M Nakayama, Hazuki Mizukawa and Mayumi Ishizuka
  • 学会等名: 7th International Toxicology Symposium in Africa
  • 発表場所: Garden Court Hotel OR Tambo, Johannesburg, South Africa
  • 年月日: 2015-08-31
  • 国際学会 / 招待講演
• [学会発表] Vector-control pesticides disseminating via international waterways to accumulate in free-ranging freshwater and marine fish species (2015)
  • 著者名/発表者名: Lesa A Thompson, Yoshinori Ikenaka, Yared B Yohannes, Johan van Vuren, Victor Wepener, Nico J Smit, Wynand Vlok, Shouta M M Nakayama, Hazuki Mizukawa and Mayumi Ishizuka
  • 学会等名: Vth International Wildlife Management Congress
  • 発表場所: Sapporo Convention Center, Sapporo, Hokkaido, Japan
  • 年月日: 2015-07-26
  • 国際学会
• [学会発表] Vector-control pesticide DDT detected in free-ranging chickens from South Africa (2015)
  • 著者名/発表者名: Lesa A Thompson, Yoshinori Ikenaka, Yared B Yohannes, Johan van Vuren, Victor Wepener, Nico J Smit, Wynand Vlok, Shouta M M Nakayama, Hazuki Mizukawa and Mayumi Ishizuka
  • 学会等名: 24th Symposium on Environmental Chemistry
  • 発表場所: Sapporo Convention Center, Sapporo, Hokkaido, Japan
  • 年月日: 2015-06-24
• [備考] Vetmed Tox Hokkaido University
  • URL: http://tox.vetmed.hokudai.ac.jp/en/
• [備考] Vetmed Tox Hokkaido University
• [備考] http://tox.vetmed.hokudai.ac.jp/en/
• [学会・シンポジウム開催] 4th Sapporo Summer Seminar for One Health, committee chairperson for student session ”Collaboration in One Health: what’s your role?” (2016)
  • 発表場所: Hokkaido University, Sapporo, Hokkaido, Japan
  • 年月日: 2016-09-20
• [学会・シンポジウム開催] South Africa - Japan bipartite research symposium, invited presenter, “Extraneous DDT residues in free-ranging chicken products from KwaZulu-Natal, South Africa” (2016)
  • 発表場所: Hokkaido University, Sapporo, Hokkaido, Japan
• [学会・シンポジウム開催] IWMC, crayfish eradication program (2015)
  • 発表場所: Toyako-cho
  • 年月日: 2015-07-31