| 研究実績の概要 |
We have identified that there is an increase in NG2-positive cells, a marker of activated pericyte, expressed in ischemic kidney using 2-kidney 1-clip (2K1I) model as compared to control kidney. Moreover, we discovered that a part of NG2-positive cells is also expressed CD11b or F4/80, a marker of macrophage, in ischemic kidney using flow cytometry and immunohistochemistry. Furthermore, we discovered that expression of NG2/CD11b-positive or NG2/F4/80-positive cells reached the highest level on day3 after ischemia. Next, we isolated NG2-positive cells from ischemic kidney and performed phagocytic ability against fluorescent-conjugated beads. We found that some of NG2-positive cells also contained phagocytic ability against the beads. It is established that phenotype of macrophage can determine severity of renal injury after ischemia. Macrophage can be divided into M1, known as inflammatory macrophage, and M2, known as anti inflammatory macrophage. Therefore, we further checked whether NG2-positive cells are expressed the markers of M1 or M2 macrophage using real-time PCR. We found that NG2-positive cells isolated from ischemic kidney expressed similar level of TNF alpha and IL-6, which is known as M1 markers, as compared to the control kidney. In contrast, NG2-positive cells isolated from ischemic kidney contained a higher level of mannose receptor and IL-10, which is known as M2 markers, as compared to the controls, These results suggest that NG2-positive cells in ischemic kidney contain phenotype of M2 macrophage.
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