メダカゲノム情報にもとづく脊椎動物染色体再編の比較ゲノム解析

• 研究課題/領域番号: 17018016
• 研究種目: 特定領域研究
• 配分区分: 補助金
• 審査区分: 生物系
• 研究機関: 名古屋大学
• 研究代表者: 堀 寛 名古屋大学, 大学院・理学研究科, 名誉教授 (60116663)
• 研究分担者: 三谷 啓志 東京大学, 大学院・新領域創成科学研究科, 教授 (70181922) ; 佐々木 貴史 慶応大学, 医学部, 助手 (70306843) ; 清水 厚志 慶応大学, 医学部, 助手 (30327655) ; 金森 章 名古屋大学, 大学院・理学研究科, 助教 (40324389)
• 研究期間 (年度): 2005 – 2009
• 研究課題ステータス: 完了 (2009年度)
• 配分額: 93,500千円 (直接経費: 93,500千円); 2009年度: 18,000千円 (直接経費: 18,000千円); 2008年度: 18,000千円 (直接経費: 18,000千円); 2007年度: 18,800千円 (直接経費: 18,800千円); 2006年度: 18,700千円 (直接経費: 18,700千円); 2005年度: 20,000千円 (直接経費: 20,000千円)
• キーワード: メダカ / ゲノム / BACライブラリー / 染色体再編 / 比較ゲノム

研究概要

ヒトやサカナなどの脊椎動物は、そのゲノムサイズが大きく変わっている。ヒトゲノムサイズが3x 10^9塩基対であるのに、硬骨魚類のメダカはその四分の1(800Mb)、フグは八分の一(400Mb)しか持っていない。この課題ではとくにメダカLG22染色体のDNA一次配列をBAC clone配列コンテイグ法によって決定し、フグのLG22と比較し、両者のゲノムサイズが何によって変化しているかを決定した。その結果、コードされる遺伝子は両者にほとんど差がなく、両者のゲノムサイズの差は多数の反復配列の存在量の差であることが判明した。

研究成果

• [雑誌論文] Polycystic kidney disease in the medaka (Oryzias latipes) pc mutant caused by a mutation in the Gli-Similar3 (glis3) gene. (2009)
  • 著者名/発表者名: Hashimoto H, Hori H, et al.
  • 雑誌名: Plos One 4(7)
  • 査読あり
• [雑誌論文] Transgenic medaka enables easy detection of oocytes in live fish. (2009)
  • 著者名/発表者名: Kinoshita M, Kanamori A, Hori H, et al.
  • 雑誌名: Molecular Reproduction and Development 76 ページ: 202-207
  • 査読あり
• [雑誌論文] Genetic differentiation among local populations of medaka fish (Oryzias latipes) evaluated through grid- and deme-based sampling. (2009)
  • 著者名/発表者名: Katsumura, T, Mitani, H, et al.
  • 雑誌名: Gene 443 ページ: 170-177
  • 査読あり
• [雑誌論文] Comparative genomics approach to the expression of fig_alpha, one of the earliest marker genes of oocyte differentiation in medaka (Oryzias latipes) (2008)
  • 著者名/発表者名: Kanamori, A., Toyama, K., Kitagawa, S., Kamehara, A., Higuchi, T., Kinoshita, M.,, Hori, H.
  • 雑誌名: Gene 423 ページ: 180-187
  • 査読あり
• [雑誌論文] Duplicated Abd-B class genes in medaka hoxAa and hoxAb clusters exhibit differential expression patterns in pectoral fin buds (2008)
  • 著者名/発表者名: Takamatsu N, Kurosawa G, Tanaka M, Takahashi M, Inokuma R, Kanamori A, Hori H
  • 雑誌名: Development, Genes and Evolution 217 ページ: 263-273
  • 査読あり
• [雑誌論文] Comparative genomics approach to the expression of fig α, one of the earliest marker genes of oocyte differentiation inmedaka (Oryzzas latipes) (2008)
  • 著者名/発表者名: Kanamori A, Hon H, et.al.
  • 雑誌名: Gene 423 ページ: 180-187
  • 査読あり
• [雑誌論文] Transgenic medaka enables easy detection of oocytes in live fish (2008)
  • 著者名/発表者名: Kinoshita, M, Kanamon A, Hori H, et.al.
  • 雑誌名: Molecular Reproduction and Development 217 ページ: 263-273
  • 査読あり
• [雑誌論文] CYR61 is a novel gene associated with temperature-dependent changes in fish metabohsm as revealed by cDNA microarray analysis on a medaka Oryzias latipescell line (2008)
  • 著者名/発表者名: Hirayama M, Ahsan MN, Mitaru H, et.al.
  • 雑誌名: Journal of Cell Biochemistry 104 ページ: 1297-1310
  • 査読あり
• [雑誌論文] Rescue from oculocutaneous albinism type 4 using medaka slc45a2 cDNA driven by its own promoter. (2008)
  • 著者名/発表者名: Fukamachi S, Mitani H
  • 雑誌名: Geenetics 178(2): ページ: 761-769
  • 査読あり
• [雑誌論文] The DNA sequence of medaka chromosome LG22 (2007)
  • 著者名/発表者名: Sasaki T, Shimizu A, Ishikawa SK, Imai S, Asakawa S, Murayama Y, Khorasani MZ, Mitani H, Furutani-Seiki M, Kondoh H, Nanda I, Schmid M, Schartl M, Nonaka M, Takeda H, Hori H, Himmelbauer H, Shima A, Shimizu N.
  • 雑誌名: Genomics 89(1) ページ: 124-133
  • 査読あり
• [雑誌論文] The DNA sequence of medaka chromosome LG22. (2007)
  • 著者名/発表者名: Sasaki T, Hori H, et. al.
  • 雑誌名: Genomics 89(1) ページ: 124-133
  • 査読あり
• [雑誌論文] Duplicated Abd-B class genes in medaka hoxAa and hoxAb clusters exhibit differential expression patterns in pectoral fin buds (2007)
  • 著者名/発表者名: Takamatsu N, Kanamori A, Hori H
  • 雑誌名: Development, Genes and Evolution 217 ページ: 263-273
  • 査読あり
• [雑誌論文] Duplicated Abd-B class genes in medaka hoxAa and hoxAb clusters exhibit differential expression patterns in pectoral fin buds (2007)
  • 著者名/発表者名: Takamatsu N, Kurosawa G, Tanaka M, Takahashi M, Inokuma R, Kanamori A, Hori H
  • 雑誌名: Development, Genes and Evolution (in press)
• [雑誌論文] The genome size evolution of medaka (Oryzias latipes) and fugu (Takifrubripes). (2007)
  • 著者名/発表者名: Imai S, Sasaki T, Shimizu A, Asakawa S, Hori H, Shimizu N
  • 雑誌名: Genes and Genetic Systems (in press)
• [雑誌論文] Mutant analyses reveal different functions of fgfri in medaka and zebrafish despite conserved ligand-receptor relationships (2007)
  • 著者名/発表者名: Yokoi H, Shimada A, Carl M, Takashima S, Kobayashi D, Takanori Narita, Tomoko Jindo, Tetsuaki kimura, Tadao Kitagawa, Takahiro Kage, Astushi Sawada, Kiyoshi Naruse, Syuichi Asakawa, Nobuyoshi Shimizu, Hiroshi Mitani, Akihiro Shima, Makiko Tsutsumi, Hiroshi Hori, Joachim Wittbrodt, Yumiko Saga, Yuji Ishikawa, Kazuo Araki, Hiroyuki Takeda H
  • 雑誌名: Dev. Biol. (in press)
• [雑誌論文] Comparative Genomics of Medaka and Fugu. Proceedings for TODAI International Symposium of Functional Genomics of Pufferfish-Recent Advances and Perspective-. (2007)
  • 著者名/発表者名: Shimizu N, Sasaki T, Asakawa S, Shimizu A, Ishikawa SK, Imai S, Murayama Y, Himmelbauer H, Mitani M, Furutani-Seiki M, Kondoh H, Schartl M, Nonaka M, Takeda H, Hori H, Shima A
  • 雑誌名: Comp. Biochem. Physiol. (in press)
• [雑誌論文] The DNA sequence of medaka chromosome LG22. (2007)
  • 著者名/発表者名: Sasaki T, Shimizu A, Ishikawa SK, Imai S, Asakawa S, Murayama Y, Khorasani MZ, Mitani H, Furutani-Seiki M, Kondoh H, Nanda I, Schmid M, Schartl M, Nonaka M, Takeda H, Hori H, Himmelbauer H, Shima A, Shimizu N
  • 雑誌名: Genomics 89(1) ページ: 124-133
• [雑誌論文] Medaka double mutants for color interfere and leucophore free: characterization of the xanthophore-somatolactin relationship using the leucophore free gene (2006)
  • 著者名/発表者名: Fukamachi S, Wakamatsu Y, Mitani h
  • 雑誌名: Dev. Genes Evol 216(3) ページ: 152-157
  • 査読あり
• [雑誌論文] Genomic organization of the sex-determining and adjacent regions of the sex chromosomes of medaka (2006)
  • 著者名/発表者名: Kondo M, Hornung U, Nanda I, Imai S, Sasaki T, Shimizu A, Asakawa S, Hori H, Schmid M, Shimizu N, Schartl M
  • 雑誌名: Genome Res. 16(7) ページ: 815-26
  • 査読あり
• [雑誌論文] Organization and structure of hox gene loci in medaka genome and comparison with those of pufferfish and zebrafish genomes (2006)
  • 著者名/発表者名: Kurosawa G, Takamatsu N, Takahashi M, SumitomoM, SanakaE, Yamada K, Nishi K, Matsuda M, Asakawa S, Ishiguro K, KurosawaY, Shimizu N, Kohara Y, Hori H
  • 雑誌名: Gene 370 ページ: 75-82
  • 査読あり
• [雑誌論文] Organization and structure of hox gene loci in medaka genome and comparison with those of pufferfish and zebrafish genomes. (2006)
  • 著者名/発表者名: Kurosawa G, Takamatsu N, Takahashi M, Sumitomo M, Sanaka E, Yamada K, Nishi K, Matsuda M, Asakawa S, Ishiguro K, KurosawaY, Shimizu N, Kohara Y, Hori H
  • 雑誌名: Gene 370 ページ: 75-82
• [雑誌論文] Color reversion of the albino medaka fish associated with spontaneous somatic excision of the Tol-1 transposable element from the tyrosinase gene. (2006)
  • 著者名/発表者名: Tsutsumi M, Imai S, Kyono-Hamaguchi Y, Hamaguchi S, Koga A, Hori H
  • 雑誌名: Pigment Cell Res. 19(3) ページ: 243-247
• [雑誌論文] Comparative Genomics of Medaka and Fugu. Proceedings for TODAI International Symposium of Functional Genomics of Pufferfish-Recent Advances and Perspective. (2006)
  • 著者名/発表者名: Shimizu N, Sasaki T, Asakawa S, Shimizu A, Ishikawa SK, Imai S, Murayama Y, Himmelbauer H, Mitani M, Furutani-Seiki M, Kondoh H, Schartl M, Nonaka M, Takeda H, Hori H, Shima A
  • 雑誌名: Comp. Biochem. Physiol (in press)
• [雑誌論文] Functional characterization of visual opsin repertoire in Medaka (Oryzias latipes) (2006)
  • 著者名/発表者名: Matsumoto Y, Fukamachi S, Mitani H, Kawamura S
  • 雑誌名: Gene (in press)
• [雑誌論文] Temperature-dependent growth rates and gene expression patterns of various medaka Oryzias latipes cell lines derived from different populations. (2006)
  • 著者名/発表者名: Hirayama M, Mitani H, Watabe S
  • 雑誌名: J. Comp. Physiol. B (in press)
• [雑誌論文] Medaka double mutants for color mtertere ana leucophore tree : charactenzation of the xanthophore-somatolactin relationship using the leucophore tree gene. (2006)
  • 著者名/発表者名: Fukamachi S, Wakamatsu Y, Mitani H
  • 雑誌名: Dev.Genes Evol. (in press)
• [雑誌論文] Reduced expression of vps11 causes less pigmentation in medaka, Oryzias latipes (2006)
  • 著者名/発表者名: Yu JF, Fukamachi S, Mitani H, Hori H, Kanamori A
  • 雑誌名: Pigment Cell Res. 19(6) ページ: 628-634
• [雑誌論文] Genomic organization of the sex-determining and adjacent regions of the sex chromosomes of medaka. (2006)
  • 著者名/発表者名: Kondo M, Hornung U, Nanda I, Imai S, Sasaki T, Shimizu A, Asakawa S, Hori H, Schmid M, Shimizu N, Schartl M
  • 雑誌名: Genome Res. 16(7) ページ: 815-826
• [雑誌論文] Targeted reduction of the DNA methylation level with 5-azacytidine promotes excision of the medaka fish Tol2 transposable element. (2006)
  • 著者名/発表者名: Iida A, Shimada A, Shima A, Takamatsu N, Hori H, Takeuchi K, Koga A
  • 雑誌名: Genet. Res. 87(3) ページ: 187-193
• [雑誌論文] Structural components of the synaptonemal complex, SCP1 and SCP3, in the medaka fish Oryzias latipes. (2006)
  • 著者名/発表者名: Iwai T, Yoshii A, Yokota T, Sakai C, Hori H, Kanamori A, Yamashita M
  • 雑誌名: Exp. Cell Res. 312(13) ページ: 2528-2537
• [雑誌論文] Methyltestosterone efficiently induces male development in the self-fertilizing hermaphrodite fish, Kryptolebias marmoratus. (2006)
  • 著者名/発表者名: Kanamori A, Yamamura A, Koshiba S, Lee JS, Orlando EF, Hori H
  • 雑誌名: Genesis 44(10) ページ: 495-503
• [雑誌論文] Vertebrate DNA transposon as a natural mutator : the medaka fish lol2 element contributes to genetic variation without recognizable traces. (2006)
  • 著者名/発表者名: Koga A, lida A, Hori H, Shimada A, Shima A
  • 雑誌名: Mol. Biol. Evol. 23(7) ページ: 1414-1419
• [雑誌論文] Medaka unextended-fin mutants suggest a role for Hoxb8a in cell migration and osteoblast differentiation during appendage formation. (2006)
  • 著者名/発表者名: Sakaguchi S, Nakatani Y, Takamatsu N, Hori R, Kawakami A, Inohaya K, Kudo A
  • 雑誌名: Dev. Biol. : 426-38 293(2) ページ: 426-438
• [雑誌論文] Organization and structure of hox gene loci in medaka genome and comparison with those of pufferfish and zebrafish genomes. (2006)
  • 著者名/発表者名: Kurosawa G, Takamatsu N, Takahashi M, Sumitomo M, Sanaka E, Yamada K, Nishii K, Matsuda M, Asakawa S, Ishiguro K, Kurosawa Y, Shimizu N, Kohara Y, Hori H.
  • 雑誌名: Gene (in press)
• [雑誌論文] Color reversion of the albino medaka fish associated with spontaneous somatic excision of the Tol-1 transposable element from the tyrosinase gene. (2006)
  • 著者名/発表者名: Tsutsumi M, Imai S, Kyono-Hamaguchi Y, Hamaguchi S, Koga A, Hori H.
  • 雑誌名: Pigment Cell Res. (in press)
• [雑誌論文] Comparative Genomics of Medaka and Fugu. Proceedings for TODAI International Symposium of Functional Genomics of Pufferfish - Recent Advances and Perspective. (2006)
  • 著者名/発表者名: Shimizu N, Sasaki T, Asakawa S, Shimizu A, Ishikawa SK, Imai S, Murayama Y, Himmelbauer H, Mitani M, Furutani-Seiki M, Kondoh H, Schartl M, Nonaka M, Takeda H, Hori H, Shima A
  • 雑誌名: Comp.Biochem.Physiol (in press)
• [雑誌論文] Functional characterization of visual opsin repeptoire in Medaka (Oryzias latipes) (2006)
  • 著者名/発表者名: Matsumoto Y, Fukamachi S, Mitani H, Kawamura S.
  • 雑誌名: Gene (in press)
• [雑誌論文] Temperature-dependent growth rates and gene expression patterns of various medaka Oryzias latipes cell lines derived from different populations (2006)
  • 著者名/発表者名: Hirayama M, Mitani H, Watabe S.
  • 雑誌名: J.Comp.Physiol.B (in press)
• [雑誌論文] Medaka double mutants for color interfere and leucorhore free : characterization of the xanthophore-somatolactin relationship using the leucophore free gene. (2006)
  • 著者名/発表者名: Fukamachi S, Wakamatsu Y, Mitani H
  • 雑誌名: Dev.Genes Evol. (in press)
• [雑誌論文] Structural and functional evolution of three cardiac natriuretic peptides. (2005)
  • 著者名/発表者名: Inoue K, Sakamoto I, Yuge Y, Iwatani H, Yamagami S, TsuTsumi, M, Hori H, Cerra MC, Tota B, Suzuki N, Okamoto N, Takei Y
  • 雑誌名: Mol.Biol.Evol. 22・12 ページ: 2428-2434
• [雑誌論文] Reversion mutation of ib oculocutaneous albinism to wild-type pigmentation in medaka fish. (2005)
  • 著者名/発表者名: Iida A, Takamatsu N, Hori H, Wakamatsu Y, Shimada A, Shima A, Koga A.
  • 雑誌名: Pigment Cell Res. 18・5 ページ: 382-384
• [雑誌論文] A novel membrane guanylyl cyclase expressed in medaka (Oryzias latipes) intestine. (2005)
  • 著者名/発表者名: Iio K, Nakauchi M, Yamagami S, Tsutsumi M, Hori H, Naruse K, Mitani H, Shima A, Suzuki N
  • 雑誌名: Comp Biochem.Physiol.B Biochem.Mol.Biol. 140・4 ページ: 569-578
  • NAID: 120000956741
• [雑誌論文] An in vivo visual reporter system for detection of estrogen-like substances by transgenic medaka. (2005)
  • 著者名/発表者名: Kurauchi K, Nakaguchi Y, Tsutsumi M, Hori H, Kunihara R, Hashimoto H, Ohnuma R, Yamoto Y, Matsuoka S, Kawai S, Hirata T, Kinoshita M.
  • 雑誌名: Environ.Sci.Technol. 39・8 ページ: 2762-2768
• [雑誌論文] A resource for medaka gene expression patterns (2005)
  • 著者名/発表者名: Henrich T, Ramialison M, Wittbrodt B, Assouline B, Bourrat F, Berger A, Himmelbauer H, Sasaki T, Shimizu N, Westerfield M, Kondoh H, Wittbrodt J
  • 雑誌名: Bioinfomatics 21・14 ページ: 3195-3197
• [雑誌論文] Divergent expression patterns of Sox9 duplicates in teleosts indicate a lineage specific subfunctionalization (2005)
  • 著者名/発表者名: Kluver N, Kondo M, Herpin A, Mitani H, Schartl M.
  • 雑誌名: Dev.Genes Evol. 215・6 ページ: 297-305
• [雑誌論文] Genetic linkage map of medaka with polymerase chain reaction length polymorphisms. (2005)
  • 著者名/発表者名: Kimura T, Yoshida K, Shimada A, Jindo T, Sakaizumi M, Mitani H, Naruse K, Takeda H, Inoko H, Tamiya G, Shinya M
  • 雑誌名: Gene 363・1 ページ: 24-31
• [雑誌論文] Medaka receptors for somatolactin and growth hormone : phylogenetic paradox among fish growth hormone receptors. (2005)
  • 著者名/発表者名: Fukamachi S, Yada T, Mitani H.
  • 雑誌名: Genetics 171・ ページ: 1875-1883
• [学会発表] Syntheny and chromosome evolution in the Lepidoptera: Interspecies cross mapping between Papilio polytes and P. alphenor (2009)
  • 著者名/発表者名: Obara M, Furukawa M, Murayama T, Hori H.
  • 学会等名: 8th International Workshop on the Molecular Biology and Genetics of the Lepidoptera, Orthodox Academy of Crete Kolympari
  • 発表場所: Crete, Greece
• [学会発表] Syntheny and chromosome evolution in the Lepidoptera : Interspecies cross mapping between Papilio polytes and P.alphenor. (2009)
  • 著者名/発表者名: Hori H
  • 学会等名: 8^<th> International Workshop on the Molecular Biology and Genetics of the Lepidoptera.
  • 発表場所: Crete, Greece
• [学会発表] Expression of steroid hormone receptor genes in the gonads of the self-fertilizing hermaphrodite fish (2008)
  • 著者名/発表者名: Kanamon A, Hori H, et.al.
  • 学会等名: International Symposium on "Sex Determination and Gametogenesis in Fish Current Status and Future Directions"
  • 発表場所: University of Hawaii, Honolulu
• [学会発表] Cloning and expression of doublesex gene of a mimetic butterfly species, Papilio polytes (2007)
  • 著者名/発表者名: Hori H
  • 学会等名: 5th International Conference of the Biology of Butterflies
  • 発表場所: Rome, Italy
• [学会発表] Molecular phylogenetic structure of a mimetic butterfly species, Papilio polytes (2006)
  • 著者名/発表者名: Hori H, Takeda A, Inokuma R, Takahashi K, Sakamoto N, Furumoto A, Ae S.
  • 学会等名: 7th International Workshop on the Molecular Biology and Genetics of the Lepidoptera, Orthodox Academy of Crete Kolympari
  • 発表場所: Crete, Greece
• [図書] 脊椎動物のゲノム進化. 「海洋の生命史」(西田睦編) (2009)
  • 著者名/発表者名: 高松尚文、堀寛
  • 出版者: 東海大学出版会
• [図書] 小型魚類がわかる.「研究をささえるモデル生物-実験室いきものガイド」(吉川寛・堀寛編) (2009)
  • 著者名/発表者名: 堀 寛
  • 出版者: 化学同人
• [図書] 小型魚類がわかる,研究をささえるモデル生物-実験室いきものガイド(吉川寛・堀寛編) (2009)
  • 著者名/発表者名: 堀寛
  • 総ページ数: 262
  • 出版者: 化学同人