| 研究実績の概要 |
It was established that an effective and rapid detection method based on the odor fingerprint of frying oil for a real-time detection and monitoring of frying oil quality. The potential toxicities of frying oils' polar substances and oxidative products (hydroperoxides) to cells in vivo were investigated. The major primary oxidative products of the linoleic acid, 9 and 13-Hydroxyoctadecadienoic acid (9 and 13-HPODE), were synthesized using soybean lipoxygenase I-B. The 9 and 13-HPODE were also subjected to HepG 2 cells to evaluate its possible adverse effects (cell viability, changes in the enzyme expression, lipidomic analysis) . The research results of 2019 are summarized as follows:
1.The synthesized hydroperoxides have been identified by the Mass spectroscopy and NMR. Results showed that the purity of the synthesized 13-HPODE was around 95% and the yield rate was 70% above which implies the method is successful and was feasible for preparing 9 and 13-HPODE in a regular laboratory.
2.In the cells’ experiments, there were two parallel experiments carried out: one is for the PAHs and another is for the HPODEs using target cell line- HepG 2 cells. Results showed that the mRNA expression of GSTO1, SOD2, and HO-1 have increased when the cells were treated with high HPODEs concentration (100 uM), while the GPX mRNA expression was inhibited after the treatment. Results also indicated that the expression level of CYP1A1 mRNA has been significantly increased after the cells treated with high concentration of HPODEs.
|
