| 研究課題/領域番号 |
17H04652
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| 研究種目 |
基盤研究(B)
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| 配分区分 | 補助金 |
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| 応募区分 | 海外学術 |
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| 研究分野 |
応用薬理学
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| 研究機関 | 大阪市立大学 |
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研究代表者 |
五十棲 理恵 大阪市立大学, 大学院医学研究科, 講師 (30550355)
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| 研究分担者 |
寺本 勲 (木俣勲) 大阪市立大学, 大学院医学研究科, 研究員 (20153174)
上村 春樹 長崎大学, 熱帯医学研究所, 講師 (60184975)
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| 研究期間 (年度) |
2017-04-01 – 2020-03-31
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| 研究課題ステータス |
採択後辞退 (2019年度)
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| 配分額 *注記 |
17,940千円 (直接経費: 13,800千円、間接経費: 4,140千円)
2019年度: 5,200千円 (直接経費: 4,000千円、間接経費: 1,200千円)
2018年度: 5,590千円 (直接経費: 4,300千円、間接経費: 1,290千円)
2017年度: 7,150千円 (直接経費: 5,500千円、間接経費: 1,650千円)
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| キーワード | Plasmodium falciparum / malaria prophylaxis / doxycycline / drug resistance / マラリア / 予防投薬 / 薬剤耐性 / pfTetQ / 熱帯熱マラリア / 分子疫学的研究 |
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| 研究実績の概要 |
Objectives: Although doxycycline is recommend as malaria prophylaxis, a French soldier fatal case of malaria prophylactic failure was reported in Africa, despite the patient’s collective doxycycline intake as malaria prophylaxis in 2014. In the same year, it was reported that pfTetQ gene of KYNNNN motif repeats of <3 was correlated to a resistant phenotype of doxycycline, with an odds ratio of 15 in the study from Kenya. Therefore we conducted surveillance for polymorphism in the Plasmodium falciparum pfTetQ gene of KYNNNN motif repeats in samples from Kenya previously collected in western Kenya.
Methods: Screening for pfTetQ gene of KYNNNN motif repeats was conducted by sequencing using previously reported method.
Results: A total of 747 samples were analyzed. In total, 321 samples (43.0%) were <3 pfTetQ KYNNNN motif repeats, which is predictive of in vitro parasites related to doxycycline resistance. Two-hundred and forty-two (32.4%) samples more than 3 repeats and the remaining 184 (24.6%) were mixed type caused by multiple isolates with different genotype infection.
Conclusions: pfTetQ gene of KYNNNN motif repeats of <3 in P. falciparum was widely distributed in malaria endemic Kenya. Precise evaluation of the efficacy of doxycycline as a malarial prophylaxis is urgently needed for travelers who visit this area.
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| 現在までの達成度 (段落) |
翌年度、交付申請を辞退するため、記入しない。
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| 今後の研究の推進方策 |
翌年度、交付申請を辞退するため、記入しない。
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