研究課題
基盤研究(C)
In order to study thermodynamics of protein misfolding-induced aggregation and establish a new assay, isothermal titration calorimetry (ITC) was performed on amyloidogenesis of several amyloidogenic proteins and peptides. I revealed that amyloid formation resulited in exothermic heat after the lag time, and obtained thermodynamic parameters such as the change in enthalpy using in-house algorithms. I further challenged to elucidate the kinetic property of amyloid formation, and could calculate the rate constant of nucleation and elongation of amyloid fibrillation. Energy landscape built using these parameters revealed the polymorphic amyloidogenesis of b2m.ITC-based assay provides a powerful method for the study of the thermodynamics, kinetics, and mechanism of protein aggregation.
すべて 2018 2017
すべて 雑誌論文 (9件) (うち国際共著 8件、 査読あり 9件、 オープンアクセス 3件) 学会発表 (15件) (うち国際学会 4件、 招待講演 11件)
Nature Nanotechnology
巻: - 号: 9 ページ: 812-818
10.1038/s41565-018-0179-y
Scientific Reports
巻: 8 号: 1 ページ: 9725-9725
10.1038/s41598-018-27860-w
Chem Commun (Camb).
巻: 57 号: 57 ページ: 7995-7998
10.1039/c8cc02718h
Nucleic Acids Research
巻: 46 (W1) 号: W1 ページ: 315-322
10.1093/nar/gky497
Biochimica et Biophysica Acta (BBA) - Biomembranes
巻: － 号: 9 ページ: 30130-30135
10.1016/j.bbamem.2018.04.012
Protein Science
巻: － 号: 7 ページ: 1151-1165
10.1002/pro.3395
巻: 1860 号: 3 ページ: 757-766
10.1016/j.bbamem.2017.12.011
Physical Chemistry Chemical Physics
巻: 19 号: 24 ページ: 16257-16266
10.1039/c6cp07774a
International Journal of Biological Macromolecules
巻: 102 ページ: 1241-1249
10.1016/j.ijbiomac.2017.05.001
