昆虫の光周性と概日振動の分子生物学

• 研究課題/領域番号: 18380043
• 研究種目: 基盤研究(B)
• 配分区分: 補助金
• 応募区分: 一般
• 研究分野: 応用昆虫学
• 研究機関: 神戸大学
• 研究代表者: 竹田 真木生 神戸大学, 大学院・農学研究科, 教授 (20171647)
• 研究期間 (年度): 2006 – 2009
• 研究課題ステータス: 完了 (2009年度)
• 配分額: 18,430千円 (直接経費: 15,100千円、間接経費: 3,330千円); 2009年度: 4,810千円 (直接経費: 3,700千円、間接経費: 1,110千円); 2008年度: 4,810千円 (直接経費: 3,700千円、間接経費: 1,110千円); 2007年度: 4,810千円 (直接経費: 3,700千円、間接経費: 1,110千円); 2006年度: 4,000千円 (直接経費: 4,000千円)
• キーワード: 光周性 / 概日振動 / メラトニン合成系 / 光受容 / 変態 / 休眠 / 表現型多型 / N-acetyltransferease (NAT) / メラトニン / long wave opsin / action sp[ectra / 卵休眠 / Cycle / 概日時計 / NAT / serotonin / 5HT receptor / UVB / opsin / マダラスズ / pars intercerebralis / 摂食 / セロトニン / N-アセチル転移酵素(NAT) / 神経分泌 / Rab蛋白質 / PTTH / CYCLE / 神経伝達 / PERIOD / カゼインキナーゼ

研究概要

光周性は、生物が日長を測るシステムで、生物学的に、かつ農業上も重要であるが、物質的な制御機構は分っていない。本研究は、光受容、内分泌機構と、概日振動機構に関連する遺伝子と、その産物の動態と発現箇所の解析から、光周性の構造と調節機構を解明しようとした。概日振動の因子の脳内局在性は、種と、発育ステージで、また近縁の系統間でも大きく異なった。概日時計の下流にメラトニン合成酵素の一つ、N-アセチル転移酵素(NAT)遺伝子の転写制御があることが、この遺伝子の上流制御域の構造から示唆された。また、メラトニン受容体が出力系の前胸腺刺激ホルモン産生細胞に存在することから、環境情報がメラトニン系によって、時計の同調に使われている可能性を示唆した。メラトニン系が、光周情報の計数器なら、光情報と時計、出力系をつなぐ鍵的な因子となり、光周性の重要な機能中心を統合すると考えらる。Blue wave opsinとNAT活性、メラトニンとPTTHの分泌機構の調節は連関し、NAT細胞がPER細胞と完全に一致した局在性を示し、メラトニン合成系で光周システムが統合されている。

研究成果

• [雑誌論文] Serotonin-and Putative Serotonin Receptor-like Immunohistochemical Reactivities in the Ground Crickets Dianemobius nigrofasciatus and Allonemobius allardi. (2010)
  • 著者名/発表者名: Shao Qi-Miao, Fauda Mohammad Ali, Makio Takeda
  • 雑誌名: Accepted for publication; Journal of Insect Physiology
• [雑誌論文] Action spectrum for the suppression of arylalkylamine N-acetyltransferase activity in the two-spotted spider mite Tetranychus urticae Photochemistry and (2009)
  • 著者名/発表者名: Suzuki, T., N. Izawa, T. Takashima, M. Watanabe, M. Taked
  • 雑誌名: Photobiology (in press)
• [雑誌論文] Utilizing LED technology for arthropod pest control (2009)
  • 著者名/発表者名: Suzuki, T., Takeda, M.
  • 雑誌名: In "Light-emitting Diodes: Research, Technology and Applications" Nova Science Publishers
• [雑誌論文] Pars intercerebralis as a modulator of locomotor rhythms and feeding in the American cockroach, Periplaneta americana. (2009)
  • 著者名/発表者名: Matsui, T., Matsumoto, Sakai T., Satake H., Takeda M.
  • 雑誌名: Physiology & Behavior 96 ページ: 548-556
• [雑誌論文] Characterization of arylalkylamine N-acetyltransferase (NAT) activities and action spectrum for suppression in the band-legged cricket (2009)
  • 著者名/発表者名: Izawa N, Suzuki T, Watanabe M, Takeda M
  • 雑誌名: Dianemobius nigrofasciatus (Orthoptera: Grillidae) Comparative Biochemistry and Physiology B 152 ページ: 346-351
• [雑誌論文] Takeda M. (2009)Putative regulatory mechanism of prothoracicotropic hormone (PTTH) secretion in the American cockroach, Periplaneta americana as inferred from co-locatization of Rab8, PTTH and protein kinase C in neurosectretory cells. (2009)
  • 著者名/発表者名: Hiragaki, S., Uno T., Takeda M.
  • 雑誌名: Cell and Tissue Research 335 ページ: 607-615
• [雑誌論文] UV tolerance in the two-spotted spider mite, Tetranychus urticae (accepted for publication) (2009)
  • 著者名/発表者名: Suzuki, T., Watanabe, M., Takeda, M
  • 雑誌名: Journal of Insect Physiology 55 ページ: 649-654
• [雑誌論文] Phosphorylation of small GTPase Rab proteins from Bombyx mori( Lepidoptera: Bombycidae) (2009)
  • 著者名/発表者名: Uno, T, Hata, K., Trang, L.T.D., Hiragaki, S., Nakada, T., Nakamura, M., Uno, Y., Yamagata, H., Kanamaru, K., Takeda, M., Matsubara M
  • 雑誌名: Eur. J. Entomol 106 ページ: 499-506
• [雑誌論文] Characterization of arylalkylamine N-acetyltransferase(NAT)activities and action spectrum for suppression in the band-legged cricket, Dianemobius nigrofasciatus(Orthoptera : Grillidae) (2009)
  • 著者名/発表者名: Izawa N, Suzuki T, Watanabe M, Takeda M.
  • 雑誌名: Comparative Biochemistry and Physiology B 152 ページ: 346-351
  • 査読あり
• [雑誌論文] Action spectrum for the suppression of arylalkylamine N-acetyltransferase activity in the two-spotted spider mite Tetranychus urticae (2009)
  • 著者名/発表者名: Suzuki, T., N.Izawa, T.Takashima, M.Watanabe, M.Takeda
  • 雑誌名: Photochemistry and Photobiology 85 ページ: 214-219
  • 査読あり
• [雑誌論文] Putative regulatory mechanism of prothoracicotropic hormone(PTTH)secretion in the American cockroach, Periplaneta americana as inferred from co-locatization of Rab8, PTTH and protein kinase C in neurosectretory cells (2009)
  • 著者名/発表者名: Hiragaki, S., Uno T., Takeda M.
  • 雑誌名: Cell and Tissue Research 335 ページ: 607-615
  • 査読あり
• [雑誌論文] The pars intercerebralis as a modulator of locomotor rhythms and feeding in the American cockroach (2009)
  • 著者名/発表者名: Matsui,T., Matsumoto, Sakai T., SatakeH. Takeda M.
  • 雑誌名: Periplaneta americana. Physiology & Behavior 96 ページ: 548-556
• [雑誌論文] Characterization ofarylalkylamine N-acetyltransferase (NAT)activities and action spectrum forsuppression in the band-legged cricket (2009)
  • 著者名/発表者名: Izawa N, Suzuki T, Watanabe M, TakedaM.
  • 雑誌名: Dianemobius nigrofasciatus (Orthoptera : Grillidae) Comparative Biochemistry and Physiology B 152 ページ: 346-351
• [雑誌論文] Putative regulatory mechanism of prothoracicotropic hormone (PTTH) secretion in the American cockroach (2009)
  • 著者名/発表者名: Hiragaki, S., UnoT., TakedaM.
  • 雑誌名: Periplaneta americana as inferred from co-locatization of Rab8, PTTH and protein kinase C in neurosectretory cells. Celland Tissue Research 335 ページ: 607-615
• [雑誌論文] Expression analyses of casein kinase 2alpha and casein kinase 2alphan the silkmoth, Bombyx mori. (2008)
  • 著者名/発表者名: Iwai S, Trang LTD, Takeda M
  • 雑誌名: Comparative Biochemistry and Physiology, Part B 149 ページ: 38-46
• [雑誌論文] Co-localization and unique distributions of two clock proteins CYCLE and CLOCK in the cephalic ganglia of the ground cricket (2008)
  • 著者名/発表者名: Shao, Q-M, Hiragaki, S, Takeda, M
  • 雑誌名: Allonemobius allardi Cell and Tissue Research 331 ページ: 435-446
• [雑誌論文] Molecular structural, expression patterns and localization of the circadian transcription modulator CYCLE in the cricket (2008)
  • 著者名/発表者名: Shao, Q-M, Hiragaki, S, Takeda, M
  • 雑誌名: Dianemobius nigrofasciatus Journal of Insect Physiology 54 ページ: 403-413
• [雑誌論文] Effects of light quality and intensity on diapause induction in the two-spotted spider mite (2008)
  • 著者名/発表者名: Suzuki T, Fukunaga Y, Amano H, Takeda M, Goto E
  • 雑誌名: Tetranychus urticae (Applied Entomology and Zoology 43,(2) ページ: 213-218
• [雑誌論文] UV radiation elevates arylalkylamine N-acetyltransferase activity and melatonin content in the two-spotted spider mite, Tetranychus urticae (2008)
  • 著者名/発表者名: Suzuki, T., Takashima, T., N. Izawa, M. Watanabe, M. Takeda
  • 雑誌名: Journal of Insect Physiology 54 ページ: 1168-1174
• [雑誌論文] Molecular structural, expression patterns and localization of the circadian transcription modulator CYCLE in the cricket, Dianemobius nigrofasciatus. (2008)
  • 著者名/発表者名: Shao, Q.-M., Hiragaki, S., Takeda, M.
  • 雑誌名: Journal of Insect Physiology 54 ページ: 403-413
• [雑誌論文] Expression analyses of casein kinase 2alpha and casein kinase 2alphan the silkmoth, Bombyx mori (2008)
  • 著者名/発表者名: Iwai S, Trang LTD, Takeda M.
  • 雑誌名: Comparative Biochemistry and Physiology Part B149 ページ: 38-46
• [雑誌論文] Co-localization and unique distributions of two clock proteins CYCLE and CLOCK in the cephalic ganglia of the ground cricket (2008)
  • 著者名/発表者名: Shao, Q-M, Hiragaki S, Takeda, M
  • 雑誌名: Allonemobius allardi Cell and Tissue Research 331 ページ: 435-446
• [雑誌論文] Molecular structure, expression patterns and localization of the circadian transcription modulator CYCLE in the cricket (2008)
  • 著者名/発表者名: Shao, Q-M, Hiragaki,S, Takeda, M
  • 雑誌名: Dianemobius nigrofasciatus Journal of InsectPhysiology 54 ページ: 403-413
• [雑誌論文] Expression analyses of casein kinase 2 alpha and casein kinase 2a and casein kinase 2βin the silkmoth Bombyx mori (2008)
  • 著者名/発表者名: Iwai s, Tran LTD, Takeda M.
  • 雑誌名: Comparative Biochemistry and Physiology B 14 9 ページ: 38-46
  • 査読あり
• [雑誌論文] Co-localization and unique distributions of two clock proteins CYCLE and CLOCK in the cephalic ganglia of the ground cricket, Allonemobiusallardi (2008)
  • 著者名/発表者名: Shao X-Q, Hiragaki s, Takeda M
  • 雑誌名: CeU and Tissue Research 3 3 1 ページ: 435-446
  • 査読あり
• [雑誌論文] Molecular structure, expression patterns and localization of the circadian transcription modulator CYCLE in the cricket, Dianemobius nigrofasciatus (2008)
  • 著者名/発表者名: Shao X-Q, Hiragaki s, Takeda M
  • 雑誌名: Journal of Insect Physiology 5 4 ページ: 403-413
  • 査読あり
• [雑誌論文] Expression analyses of casein kinase 2alpha and casein kinase 2alphan the silkmoth, Bombyx mori (2008)
  • 著者名/発表者名: Iwai S
  • 雑誌名: Comparative Biochemistry and Physiology, Part B 149 ページ: 38-46
  • 査読あり
• [雑誌論文] Co-localization and unique distributions of two clock proteins CYCLE and CLOCK in the cephalic ganglia of the ground cricket, Allonemobius allardi (2008)
  • 著者名/発表者名: )Shao, Q-M
  • 雑誌名: Cell and Tissue Research 331 ページ: 435-446
  • 査読あり
• [雑誌論文] Molecular structural, expression patterns and localization of the circadian transcription modulator CYCLE in the cricket, Dianemobius nigrofasciatus (2008)
  • 著者名/発表者名: )Shao, Q-M
  • 雑誌名: Journal of Insect Physiology, 54 ページ: 403-413
  • 査読あり
• [雑誌論文] Molecular characterization and distribution of cycle protein from Athalia rosae. (2007)
  • 著者名/発表者名: Bembenek, J, Itokawa, K, Hiragaki, S., Shao, Q., M, Tufail, M., Takeda, M.
  • 雑誌名: J. Insect Physiol. 53 ページ: 418-427
• [雑誌論文] Effects of extending the light phase on diapause induction in a Japanese population of the two-spotted spider mite, Tetranychus urticae. (2007)
  • 著者名/発表者名: Suzuki, T., Amano A., Goto E., Takeda, M., Kozai, T.
  • 雑誌名: Experimental and Applied Acarology 42 ページ: 131-138
• [雑誌論文] Expression analysis of two types of transcripts from circadian output gene lark in Bombyx mori. (2007)
  • 著者名/発表者名: Iwai, S., Takeda, M.
  • 雑誌名: Comp. Biochem. Physiol. B 146 ページ: 470-476
• [雑誌論文] Cloning and characterization of insect arylalkylamine N-acetyltransferase for Bombyx mori. (2007)
  • 著者名/発表者名: Tsugehara, T, Iwai, S, Fujiwara, Y, Mita, K., Takeda, M
  • 雑誌名: Comp. Biochem. Physiol. B 147 ページ: 358-36
• [雑誌論文] Arylalkylamine N-acetyltransferase in insects and its regulatory role in circadian oscillation (2007)
  • 著者名/発表者名: Bembenek J., Tsugehara T., Ichihara N., Takeda M.
  • 雑誌名: Trends in Entomology 4(An InvitedReview) ページ: 1-17
• [雑誌論文] Expression analysis of two types of transcripts from circadian output gene lark in Bombyx mori (2007)
  • 著者名/発表者名: Iwai S, Takeda M.
  • 雑誌名: Comparative Biochemistry and Physiology B 146 ページ: 470-476
• [雑誌論文] M Cloning and characterization of insect arylalkylamine N-acetyltransferase fro Bombyx mori (2007)
  • 著者名/発表者名: Tsugehara T, Iwai S, Fujiwara Y, Mita K, Takeda
  • 雑誌名: Comparative Biochemistry and Physiology B. 147 ページ: 358-366
• [雑誌論文] Molecular characterization and distribution of cycle protein from Athalia rosae (2007)
  • 著者名/発表者名: Bembenek J, Itokawa K, Hiragaki S., ShaoQ.M, Tufail M., Takeda M.
  • 雑誌名: J. Insect Physiol. 53 ページ: 418-427
• [雑誌論文] Cloning and characterization of insect arylalkylamine N-acetyltransferase from Bombyx mori (2007)
  • 著者名/発表者名: Tsugehara T,
  • 雑誌名: Comp. Biochem. Physiol. B 第147巻 ページ: 358-366
  • 査読あり
• [雑誌論文] Molecular characterization and distribution of cycle protein from Athalia rosae (2007)
  • 著者名/発表者名: Bembenek J,
  • 雑誌名: J. Insect Physiol. 第53巻 ページ: 418-427
  • 査読あり
• [雑誌論文] Effects of extending the light phase on diapause induction in a Japanese population of the two-spotted spider mite, Tetranychus urticae (2007)
  • 著者名/発表者名: Suzuki, T,
  • 雑誌名: Exp. Appl. Acarol. 42; ページ: 131-138
  • 査読あり
• [雑誌論文] Molecular characterization and distribution of cycle protein from Athalia rosae. (2007)
  • 著者名/発表者名: Bembenek J, Itokawa K, Hiragaki S., Shao Q.M, Tufail M., Takeda M
  • 雑誌名: J. Insect Physiol. (印刷中)
• [雑誌論文] Expression analysis of two types of transcripts from circadian output gene lark in Bombyx mori (2007)
  • 著者名/発表者名: Iwai S, Takeda M.
  • 雑誌名: Comp.Biochem.Physiol (印刷中)
• [雑誌論文] Neurohormones as putative circadian clock output signals in the central nervous system of two cricket species. (2006)
  • 著者名/発表者名: Sehadova, H., Shao, Q.-M., Sehnal, F., Takeda, M.
  • 雑誌名: Cell Tiss. Res., Published online
• [雑誌論文] Casein kinase I in the silkworm, Bombyx mori: Structure and possible roles in circadian timing and development. (2006)
  • 著者名/発表者名: Trang, L.T.D., Sehadova, H., Ichihara, N., Iwai, S., Mita, K., Takeda, M.
  • 雑誌名: J. Biol. Rhythms 21 ページ: 335-349
• [雑誌論文] Structure and expressions of two circadian clock genes, period and timeless in the commercial silkmoth, Bombyx mori. (2006)
  • 著者名/発表者名: Iwai, S., Fukui, Y, Fujiwara, Y., Takeda, M.
  • 雑誌名: J. Insect Physiol. 52
• [雑誌論文] A non-invasive monitoring of developmental changes in pupal case of Antheraea pernyi by near infrared spectroscopy. (2006)
  • 著者名/発表者名: Tsugehara, T, Tsenkova, R., Takeda, M.
  • 雑誌名: International Journal of Wild Sikmoths and Silk 11 ページ: 14-20
  • NAID: 10022022525
• [雑誌論文] Neurohormones as putative circadian clock output signals in the central nervous system of two cricket species (2006)
  • 著者名/発表者名: Sehadova H., Shao Q.-M., Sehnal F, Takeda M.
  • 雑誌名: Cell Tiss. Res 328 ページ: 239-255
• [雑誌論文] A non-invasive monitoring of developmental changes in pupal case of Antheraea pernyi by near infrared spectroscopy (2006)
  • 著者名/発表者名: Tsugehara T, Tsenkova R, Takeda M.
  • 雑誌名: International Journal of Wild Sikmoths and Silk. 11 ページ: 14-20
  • NAID: 10022022525
• [雑誌論文] Casein kinase I in the Silkworm, Bombyx mori : Structure and possible roles in circadian timing and development (2006)
  • 著者名/発表者名: Le Thi Dieu T, Sehadova H., Ichihara N.,Iwai S. Mita K, Takeda M.
  • 雑誌名: J. Biol. Rhythms 21 ページ: 335-349
• [雑誌論文] Structure and expressions of two circadian clock genes, period and timeless in the commercial silkmoth, Bombyx mori (2006)
  • 著者名/発表者名: Iwai S. Fukui Y, Fujiwara Y, Takeda M.
  • 雑誌名: J.Insect Physiol 52 ページ: 625-637
• [雑誌論文] Immunoreactivities to 3 circadian clock proteins in 2 ground crickets suggest interspecific diversity of the circadian clock structure (2006)
  • 著者名/発表者名: Shao Q.-M., Sehadova H., Ichihara N, Sehnal F., Takeda M.
  • 雑誌名: J. Biol. Rhythms 21 ページ: 1-14
• [雑誌論文] Neurohormones as putative circadian clock output signals in the central nervous system of two cricket species (2006)
  • 著者名/発表者名: Sehadova H., Shao Q.-M., Sehnal F, Takeda M.
  • 雑誌名: Cell Tiss. Res. 328 ページ: 239-255
• [雑誌論文] Casein kinase I in the Silkworm, Bombyx mori : Structure and possible roles in circadian timing and development (2006)
  • 著者名/発表者名: Le Thi Dieu T, Sehadova H., Ichihara N., Iwai S., Mita K, Takeda M.
  • 雑誌名: J. Biol. Rhythms 21 ページ: 335-349
• [雑誌論文] Structure and expressions of two circadian clock genes, period and timeless in the commercial silkmoth, Bombyx mori. (2006)
  • 著者名/発表者名: Iwai S., Fukui Y, Fujiwara Y, Takeda M.
  • 雑誌名: J.Insect Physiol. 52 ページ: 625-637
• [雑誌論文] Arylalkylamine N-acetyltransferase in insects and its regulatory role in circadian oscillation.
  • 著者名/発表者名: Bembenek, J., Tsugehara, T., Ichihara, N., Takeda, M.
  • 雑誌名: Trends in Entomology 4(in press)(An Invited Review)
• [雑誌論文] Effects of light quality and intensity on diapause induction in the two-spotted spider mite, Tetranychus urticae.
  • 著者名/発表者名: Suzuki, T., Fukunaga, Y., Amano, H., Takeda, M., Goto, E.
  • 雑誌名: Applied Entomology and Zoology (in press)
  • NAID: 110006684222
• [雑誌論文] Molecular Structure of Insect Circadian Clock.
  • 著者名/発表者名: Trang, L.T.D., Shao, Q.-M., Takeda, K.
  • 雑誌名: Short Views on Insect Molecular Bioloy(Published by Insect Molecular Biology Unit, Bharathidasa Uni, India(Editors, Chandrasekar R, Krishnan, M)) (in press)
• [雑誌論文] Diapause-inducing signals prolong nymphal development in the two-spotted spider mite, Tetranychus urticae.
  • 著者名/発表者名: Suzuki T, Takeda M
  • 雑誌名: Physiological Entomology 34 ページ: 278-283
• [雑誌論文] Takeda M. Molecular cloning and expression analysis of two putative serotonin receptors in the brain of Antheraea pernyi pupa.
  • 著者名/発表者名: Hiragaki, S., Kawabe Y.
  • 雑誌名: International Journal of Wild Sikmoths and Silk
• [雑誌論文] Molecular cloning and expression analysis of two putative serotonin receptors in the brain of Antheraea pernyi pupa
  • 著者名/発表者名: Hiragaki,S., Kawabe Y., Takeda M.
  • 雑誌名: International Journal of Wild Sikmoths and Silk (accepted for publication)
  • NAID: 10029603134
• [雑誌論文] Takeda Molecular cloning and expression analysis of two putative serotonin receptors in the brain of Antheraea pernyi pupa.
  • 著者名/発表者名: )S. Hiragaki
  • 雑誌名: .International Journal of Wild Sikmoths and Silk (印刷中)(未定)
  • 査読あり
• [学会発表] ゴキブリの脳間部は概日リズムと摂食を制御する (2010)
  • 著者名/発表者名: 竹田真木生
  • 学会等名: 第53回日本応用動物昆虫学会大会(札幌)
  • 発表場所: 北海道大学(北海道)
• [学会発表] 昆虫の概日振動と光周性に関する-連の研究 (2008)
  • 著者名/発表者名: 竹田真木生
  • 学会等名: 日本応用動物昆虫学会<学会賞講演>
  • 発表場所: 宇都宮大学
  • 年月日: 2008-03-26
• [学会発表] ナンダ・ハムナー・プロトコールなどー光周性研究のためのツール (2008)
  • 著者名/発表者名: 竹田真木生
  • 学会等名: 時間生物学事典(石田直理雄・本間研一編)
  • 発表場所: 朝倉書店
• [図書] Springer Verlag (2009)
  • 著者名/発表者名: Ozoe, Y., Takeda, M., Matsuda, K
  • 総ページ数: 32
  • 出版者: In "Biorational Control of Arthropod Pests : Application and Resistance Management"(Ed. Ishaaya, I.)
• [図書] Short View of Insect Molecular Biology (2009)
  • 著者名/発表者名: Trang LTD, Slao QM, Takeda M
  • 出版者: Molecular structure of insect circadian clock
• [図書] ナンダ・ハムナ-・プロトコ-ルなど-光周性研究のためのツ-ル。時間生物学事典(石田直理雄・本間研一) (2008)
  • 著者名/発表者名: 竹田真木生
  • 出版者: 朝倉書店
• [図書] 多次元のコミュニケーション (2006)
  • 著者名/発表者名: 竹田真木生 (分担)
  • 総ページ数: 17
  • 出版者: 大学教育出版
• [図書] Molecular Structure of Insect Circadian Clock In : "Short Views on Insect Molecular Bioloy"(Editors, ChandrasekarR, Krishnan, M), Clock In : "Short Views on InsectMolecular Bioloy"
  • 著者名/発表者名: Le Thi Dieu Trang, Shao Q.-M., Takeda M.
  • 出版者: Published by InsectMolecular Biology Unit, Bharathidasa Uni, India Chapter 2 (印刷中)
• [図書] Short Views on Insect Molecular Bioloy
  • 著者名/発表者名: Le Thi Dieu Trang
  • 出版者: Insect Molecular Biology Unit, Bharathidasa University, India(印刷中)