炎症性腸疾患における腸管上皮細胞間接着蛋白の研究

• 研究課題/領域番号: 18590695
• 研究種目: 基盤研究(C)
• 配分区分: 補助金
• 応募区分: 一般
• 研究分野: 消化器内科学
• 研究機関: 大阪市立大学
• 研究代表者: 押谷 伸英 大阪市立大学, 大学院・医学研究科, 准教授 (20231235)
• 研究期間 (年度): 2006 – 2008
• 研究課題ステータス: 完了 (2008年度)
• 配分額: 3,830千円 (直接経費: 3,200千円、間接経費: 630千円); 2008年度: 1,430千円 (直接経費: 1,100千円、間接経費: 330千円); 2007年度: 1,300千円 (直接経費: 1,000千円、間接経費: 300千円); 2006年度: 1,100千円 (直接経費: 1,100千円)
• キーワード: 炎症性腸疾患 / クローン病 / 潰瘍性大腸炎 / 細胞間接着蛋白 / 上皮間細胞接着蛋白

研究概要

クローン病、潰瘍性大腸炎、対照大腸組織を用いて細胞間接着蛋白の免疫染色法における局在を検討した。細胞間接着蛋白に対する抗Rab13 抗体、抗VASP 抗体、抗リン酸化VASP 抗体、抗ZO-1 抗体を使用した。対照正常粘膜ではZO-1、VASP、Rab13 はタイトジャンクションに一致して染色されたが、クローン病ではZO-1、VASP、Rab13 ともに細胞質側への変位をみとめ、Rab13、ZO-1 は杯細胞内の粘液も染色された。潰瘍性大腸炎では変位は認められず、対照正常粘膜と同様に染色された。これらにより、クローン病において、細胞間接着蛋白であるRab13、VASP の変位はクローン病の腸管粘膜の透過性亢進に関与し、クローン病の病態に寄与していることが示唆された

研究成果

• [雑誌論文] Development of pouchitis with combination therapy with Peg-int erferon alpha-2b and ribavirin for chronic hepatitis C in a patient with ulcerative colitis who underwent pouch surgery (2009)
  • 著者名/発表者名: K Morimoto, H Yamagami, S Hosomi, M Ohira, T Suekane, N Kamata, M Sogawa, K Watanabe, K Tominaga, T Watanabe, Y Fujiwara, A Tamori, N Oshitani, T Arakawa
  • 雑誌名: Am J Gastroenterol. (In press)
  • 査読あり
• [雑誌論文] Phenotypical and functional study of ghrelin and its receptor in thepathogenesis of Crohn's disease (2008)
  • 著者名/発表者名: Hosomi S, Oshitani N, Arakawa T.
  • 雑誌名: Inflamm Bowel Dis 14(9) ページ: 1205-1213
  • 査読あり
• [雑誌論文] Proliferation of immature plasma cells in pouchitis mucosa in patients withulcerative colitis (2008)
  • 著者名/発表者名: Hirata N, Oshitani N, Arakawa T.
  • 雑誌名: Inflamm Bowel Dis 14(8) ページ: 1084-1090
  • 査読あり
• [雑誌論文] Usefulness of magnetic resonance imaging for detection of asymptomaticosteonecrosis of the femoral head inpatients with inflammatory bowel diseaseon longterm corticosteroid treatment (2008)
  • 著者名/発表者名: Kamata N, Oshitani N, Arakawa T.
  • 雑誌名: Scandinavian journal of gastroenterology 43(3) ページ: 308-313
  • 査読あり
• [雑誌論文] Proliferation of immature plasma cells in pouchitis mucosa in patients with ulcerative colitis. (2008)
  • 著者名/発表者名: Hirata N, Oshitani N, Kamata N, Sogawa M, Yamagami H, Watanabe K, Watanabe T, Tominaga K, Fujiwara Y, Maeda K, Hirakawa K, Arakawa T.
  • 雑誌名: Inflamm Bowel Dis. 14(8) : ページ: 1084-1090
  • 査読あり
• [雑誌論文] Phenotypical and functional study of ghrelin and its receptor in the pathogenesis of Crohn' s disease. (2008)
  • 著者名/発表者名: Hosomi S, Oshitani N, Kamat a N, Sogawa M, Yamagami H, Watanabe K, Tominaga K, Watanabe T, Fujiwara Y, Maeda K, Hirakawa K, Arakawa T.
  • 雑誌名: Inflamm Bowel Dis. 14(9) : ページ: 1205-1213
  • 査読あり
• [雑誌論文] Combined use of factor XIII and endoscopic balloon dilatation in a patient with Crohn' s disease, duodenal stenosis, and associated internal fistulas : the efficacy of coagulation factor XIII for the internal fistulas. (2008)
  • 著者名/発表者名: Kamata N, Oshitani N, Suekane T, Sogava M, Yamagami H, Watanabe K, Fujiwara Y, Arakawa T.
  • 雑誌名: Am J Gastroenterol. 103(6) : ページ: 1573-1574
  • 査読あり
• [雑誌論文] Usefulness of magnetic resonance imaging for detection of asymptomatic osteonecrosis of the femoral head in patients with inflammatory bowel disease on long-term corticosteroid treatment. (2008)
  • 著者名/発表者名: Kamata N, Oshitani N, Sogawa M, Yamagami H, Watanabe K, Fujiwara Y, Arakawa T.
  • 雑誌名: Scand J Gastroenterol. 43(3) : ページ: 308-313
  • 査読あり
• [雑誌論文] Usefulness of Double-Balloon Endoscopy in the Diagnosis of Mal ignant Small-Bowel Tumors (2008)
  • 著者名/発表者名: Yamagami H, Oshitani N, Hosomi S, Suekane T, Kamata N, Sogawa M, Okazaki H, Watanabe K, Tominaga K, Watanabe T, Fujiwara Y, Arakawa T
  • 雑誌名: CLINICAL GASTROENTEROLOGY AND HEPATOLOGY 6(18) ページ: 1202-1205
  • 査読あり
• [雑誌論文] Differential expression of vasoactive intestinal peptide receptor 1 expression ininflammatory bowel disease (2007)
  • 著者名/発表者名: Yukawa T, Oshitani N, Arakawa T.
  • 雑誌名: Int J Mol Med ページ: 161-167
  • 査読あり
• [雑誌論文] The production of interferon-r-inducible protein-10(IP10)by granulocytes and monocytes isassociated with ulcerative colitis diseaseactivity (2007)
  • 著者名/発表者名: Noguchi A, Watanabe K, Oshitani N, Arakawa T.
  • 雑誌名: J Gastroenterol 42 ページ: 947-956
  • 査読あり
• [雑誌論文] Roles of ZO-1 and epidermal growth factor inesophageal epithelial defense against acid (2007)
  • 著者名/発表者名: Okuyama M, Fujiwara Y, Oshitani N, Arakawa T.
  • 雑誌名: Digestion 75 ページ: 135-141
  • 査読あり
• [雑誌論文] Differential expression of vasoactive intestinal peptide receptor 1 expression in in flammatory bowel disease. (2007)
  • 著者名/発表者名: Yukawa T, Oshitani N, Yamagami H, Watanabe K, Higuchi K, Arakawa T
  • 雑誌名: Int J Mol Med 120 ページ: 161-167
  • 査読あり
• [雑誌論文] Control of cell wall assembly by a histone-like protein in mycobacteria (2007)
  • 著者名/発表者名: Katsube T, Matsumoto S, Takatsuka M, Okuyama M, Ozeki Y, Naito M, Nishiuchi Y, Fujiwara N, Yoshiimura M, Tsuboi T, Torii M, Oshitani N, Arakawa T, Kobayashi K.
  • 雑誌名: J Bacteriol (in press)
  • 査読あり
• [雑誌論文] Roles of ZO-1 and epidermal growth factor in esophageal epithelial defense against acid (2007)
  • 著者名/発表者名: Okuyama M, Fujiwara Y, Tanigawa T, Watanabe K, Shiba M, Tominaga K, Watanabe T, Oshitani N, Higuchi K, Arakawa T.
  • 雑誌名: Digestion 75 ページ: 135-141
  • 査読あり
• [雑誌論文] Toll-like receptor agonists stimulated human neutrophil migration via actlvation of mitogen-activated protein kinases. (2007)
  • 著者名/発表者名: Aomatsu K, Kato T, Fujita H, Hato F, Oshitani N, Kamata N, Tamura T, Arakawa T, Kitagawa S.
  • 雑誌名: Immunology 23 ページ: 171-180
  • 査読あり
• [雑誌論文] The production of interferon-r-inducible protein-10(IP10)by granulocytes and monocytes is associated with ulcerative colitis disease activity.42:947-956 (2007)
  • 著者名/発表者名: Noguchi A, Watanabe K, Narumi S, Yamagami H, Fujiwara Y, Higuchi K, Oshitani N, Arakawa T.
  • 雑誌名: J Gastroenterol 42 ページ: 947-956
  • 査読あり
• [雑誌論文] Enhanced expressions of endothelin-converting enzyme and endothelin receptors in human colonic tissues of Crohn's disease. (2007)
  • 著者名/発表者名: Suekane T, Ikura Y, Arimot J, Nakagawa M, Kitabayashi C, Naruko T, Watanabe T, Fujiwara Y, Oshitani N, Maeda Kiyoshi, Tanzawa K, Hirakawa K, Arakawa T, Ue
  • 雑誌名: J Clin Biochem Nutr (In press)
  • NAID: 130004466501
  • 査読あり
• [雑誌論文] Distinct role of c-Jun N-terminal kinase isoforms in human neutrophil apoptosis regulated by tumor necrosis factor-α and granulocyte-macrophage colony-stimulating factor. (2007)
  • 著者名/発表者名: Kato T, Noma H, Kitagawa M, Takahashi T, Oshitani N, Kitagawa S.
  • 雑誌名: J Interf Cytok Res (In press)
  • 査読あり
• [雑誌論文] Infiltration of CD19+ plasma cells with frequent labeling of Ki-67 in corticosteroid-resistant active ulcerative colitis. (2006)
  • 著者名/発表者名: Jinno Y, Ohtani H, Nakamura S, Oki M, Maeda K, Fukushima K, Nagura H, Oshitani N, Matsumoto T, Arakawa T
  • 雑誌名: Virchows Arch 448 ページ: 412-421
• [雑誌論文] Cyclic AMP delays neutrophil apoptosis via stabilization of Mcl-1 (2006)
  • 著者名/発表者名: Kato T, Kutsuna H, Oshitani N, Kitagawa S
  • 雑誌名: FEBS lettes 580 ページ: 4582-4586
• [雑誌論文] Close association between activated platelets and neutrophils in the active phase of ulcerative colitis in humans. (2006)
  • 著者名/発表者名: Kayo S, Ikura Y, Suekane T, Shirai N, Sugama Y, Ohsawa M, Adachi K, Watanabe K, Nakamura S, Fujiwara Y, Oshitani N, Higuchi K, Maeda K, Hirakawa K, Arakawa T, Ueda M.
  • 雑誌名: Inflamm Bowel Dis 12 ページ: 727-735
• [学会発表] Phenotypical and functional study of ghrelin and its receptor in thepathogenesis of Crohn's disease (2008)
  • 著者名/発表者名: Hosomi S.
  • 学会等名: Digestive Disease Week 2008 AGA
  • 発表場所: アメリカ サンディエゴ
  • 年月日: 2008-05-25
• [学会発表] Clinical impact of double balloon endoscopy on the diagnosis ofmalignant small bowel tumors : A single center experience (2008)
  • 著者名/発表者名: Yamagami H.
  • 学会等名: Digestive Disease Week 2008 AGA
  • 発表場所: アメリカサンディエゴ
  • 年月日: 2008-05-25
• [学会発表] The efficacy of infliximab for small bowel lesions of patients withCrohn's disease according to theevaluation by using capsule endoscopy anddouble balloon enteroscopy (2008)
  • 著者名/発表者名: Watanabe K.
  • 学会等名: Digestive Disease Week 2008 ASGE
  • 発表場所: アメリカサンディエゴ
  • 年月日: 2008-05-25
• [学会発表] Phenotypical and functional study of ghrelin and its receptor in the pathogenesis of Crohn's disease (2008)
  • 著者名/発表者名: Hosomi S, Watanabe K, Hirata N, Suekane T, Kamata N, Sogawa M, Yamagami H, Tominaga K, Watanabe T, Fujiwara Y, Oshitani N, Arakawa T
  • 学会等名: Digestive Disease Week 2009 AGA
  • 発表場所: アメリカサンディェゴ
  • 年月日: 2008-05-25
• [学会発表] The efficacy of "Mother-Baby Technique" enteroscopy for patients with small bowel strictures of Crohn's disease (2008)
  • 著者名/発表者名: Hosomi S, Watanabe K, HiTata N, Suekane T, Kamata N, Sogawa M, Yamagami H, Tominaga K, Watanabe T, Fujiwara Y, Oshitani N, Arakawa T
  • 学会等名: Digestive Disease Week 2009 ASGE
  • 発表場所: アメリカサンディェゴ
  • 年月日: 2008-05-25
• [学会発表] The efficacy of infliximab for small bowel lesions of patients with Crohn's disease according to the evaluation by using capsule endoscopy and double balloon enteros copy (2008)
  • 著者名/発表者名: Watanabe K, Hosomi S, Suekane T, Kamata N, Sogawa M, Yamagami H, Tominaga K, Watanabe T, Fujiwara Y, Oshitani N, Arakawa T
  • 学会等名: Digestive Di sease Week 2009 ASGE
  • 発表場所: アメリカサンディェゴ
  • 年月日: 2008-05-25
• [学会発表] Clinical impact of double balloon endoscopy on the diagnosis of malignant Small bowel tumors : A single center experience (2008)
  • 著者名/発表者名: Yamagami H, Oshitani N, Suekane T, Kamata N, Sogawa M, Okazaki H, Watanabe K, Tominaga K, Watanabe T, Fujiwara Y, Arakawa T
  • 学会等名: Digestive Disease Week 2009 ASGE
  • 発表場所: アメリカサンディェゴ
  • 年月日: 2008-05-25
• [学会発表] Toll-like receptor agonists stimulate human neutrophil migration via activation of mitogen-activated protein kinases. (2007)
  • 著者名/発表者名: Aomatsu K, Kato T, Fujita H, Hato F, Tamura T, Suekane T, Kamata N, Sobawa M, Yamagami H, Watanabe K, Oshitani N, Arakawa T, Kitagawa S.
  • 学会等名: 12th World Congress on advanced in Oncology and 10th International Symposium on Molecular Medicine
  • 発表場所: ギリシャ
• [学会発表] Role of ghrelin in inflammatory bowel disease. (2007)
  • 著者名/発表者名: Hosomi S, Yamagami H, Suekane T, Aomatsu K, Kamata N, Sobawa M, Watanabe K, Oshitani N, Arakawa T.
  • 学会等名: 12th World Congress on advanced in Oncology and 10th International Symposium on Molecular Medicine
  • 発表場所: ギリシャ
• [学会発表] Differential expression of vasoactive intestinal peptide receptor 1 expression in inflammatory bowel disease. (2007)
  • 著者名/発表者名: Sogawa M, Yukawa T, Hosomi S, Suekane T, Aomatsu K, Kamata N, Yamagami H, Watanabe K, Oshitani N, Arakawa T.
  • 学会等名: 12th World Congress on advanced in Oncology and 10th International Symposium on Molecular Medicine
  • 発表場所: ギリシャ
• [学会発表] Phenotypical and functional study of ghrelin and its receptor in the pathogenesis of Crohn's disease. (2007)
  • 著者名/発表者名: Hosiomi S, Oshitani N, Kamata N, Sogawa M, Yamagami H, Watanabe K, Tominaga K, Watanabe T, Fujiwara Y, Maeda K, Hirakawa K, Arakawa T.
  • 学会等名: The 2nd Korea-Japan IBD Symposium
  • 発表場所: 東京