新規ワクチンのスクリーニングに有用なマラリア防御血清の探索

• 研究課題/領域番号: 19406009
• 研究種目: 基盤研究(B)
• 配分区分: 補助金
• 応募区分: 海外学術
• 研究分野: 寄生虫学(含衛生動物学)
• 研究機関: 愛媛大学
• 研究代表者: 坪井 敬文 愛媛大学, 無細胞生命科学工学研究センター, 教授 (00188616)
• 研究分担者: 竹尾 暁 愛媛大学, 無細胞生命科学工学研究センター, 講師 (40302666)
• 研究期間 (年度): 2007 – 2009
• 研究課題ステータス: 完了 (2009年度)
• 配分額: 16,770千円 (直接経費: 12,900千円、間接経費: 3,870千円); 2009年度: 4,940千円 (直接経費: 3,800千円、間接経費: 1,140千円); 2008年度: 4,810千円 (直接経費: 3,700千円、間接経費: 1,110千円); 2007年度: 7,020千円 (直接経費: 5,400千円、間接経費: 1,620千円)
• キーワード: 感染症 / マラリア / ワクチン / ゲノム / タイ / バイオテクノロジー / 国際研究者交流

研究概要

高度マラリア流行地には、マラリアに感染しても発熱等の症状が出ない無症候原虫感染者が存在している。これらの人々の血清中にはマラリア原虫の増殖を阻害する抗体が存在することが知られている。そこで本研究は、新規マラリアワクチン候補抗原の探索に用いることの出来る、防御抗体を保有する流行地住民の血清をタイ国において入手することを目的に実施した。3年間にわたるタイ国西部のマラリア流行地調査の結果、マラリア防御抗体を保有する無症候感染者血清、及びその比較対照群となる有症患者血清を入手できた。

研究成果

• [雑誌論文] Wheat germ cell-free technology for accelerating the malaria vaccine research. (2009)
  • 著者名/発表者名: Takeo S, Arumugam TU, Torii M, Tsuboi T.
  • 雑誌名: Expert Opin Drug Discov. 4(in press) ページ: 1191-1199
  • 査読あり
• [雑誌論文] Malaria ookinete surface protein-based vaccination via the intranasal route completely blocks parasite transmission in both passive and active vaccination regimens in a rodent model of malaria infection. (2009)
  • 著者名/発表者名: Arakawa T, Tachibana M, Miyata T, Harakuni T, Kohama H, Matsumoto Y, Tsuji N, Hisaeda H, Stowers A, Torii M, Tsuboi T.
  • 雑誌名: Infect Immun 77 ページ: 5496-5500
  • 査読あり
• [雑誌論文] Evidence for the transmission of Plasmodium vivax in the Republic of the Congo, west central Africa. (2009)
  • 著者名/発表者名: Culleton R, Ndounga M, Zeyrek FY, Coban C, Casimiro PN, Takeo S, Tsuboi T, Yadava A, Carter R, Tanabe K.
  • 雑誌名: J Infect Dis. 200 ページ: 1465-1469
  • NAID: 120006983581
  • 査読あり
• [雑誌論文] Sterile protection against Plasmodium knowlesi in rhesus monkeys from a malaria vaccine: comparison of heterologous prime boost strategies. (2009)
  • 著者名/発表者名: Jiang G, Shi M, Conteh S, Richie N, Banania G, Geneshan H, Valencia A, Singh P, Aguiar J, Limbach K, Kamrud KI, Rayner J, Smith J, Bruder JT, King CR, Tsuboi T, Takeo S, Endo Y, Doolan DL, Richie TL, Weiss WR.
  • 雑誌名: PLoS ONE 4(8)
  • 査読あり
• [雑誌論文] Preerythrocytic, live-attenuated Plasmodium falciparum vaccine candidates by design. (2009)
  • 著者名/発表者名: VanBuskirk KM, O'Neill MT, De La Vega P, Maier AG, Krzych U, Williams J, Dowler MG, Sacci, Jr. JB, Kangwanrangsan N, Tsuboi T, Kneteman NM, Heppner, Jr. DG, Murdock BA, Mikolajczak SA, Aly ASI, Cowman AF Kappe SHI.
  • 雑誌名: Proc Natl Acad Sci U S A. 106 ページ: 13004-13009
  • 査読あり
• [雑誌論文] Enzymatic characterization of the Plasmodium vivax chitinase, a potential malaria transmission-blocking target. (2009)
  • 著者名/発表者名: Takeo S, Hisamori D, Matsuda S, Vinetz J, Sattabongkot J, Tsuboi T.
  • 雑誌名: Parasitol Int. 58 ページ: 243-248
  • NAID: 10027669617
  • 査読あり
• [雑誌論文] Single amino acid substitution in Plasmodium yoelii erythrocyte ligand determines its localization and controls parasite virulence. (2009)
  • 著者名/発表者名: Otsuki H, Kaneko O, Thongkukiatkul A, Tachibana M, Iriko H, Takeo S, Tsuboi T, Torii M.
  • 雑誌名: Proc Natl Acad Sci U S A. 106 ページ: 7167-7172
  • NAID: 120006984096
  • 査読あり
• [雑誌論文] A small-scale systematic analysis of alternative splicing in Plasmodium falciparum. (2009)
  • 著者名/発表者名: Iriko H, Jin L, Kaneko O, Takeo S, Han ET, Tachibana M, Otsuki H, Torii M, Tsuboi T.
  • 雑誌名: Parasitol Int. 58 ページ: 196-199
  • NAID: 10027669225
  • 査読あり
• [雑誌論文] Pyruvate kinase type-II isozyme in Plasmodium falciparum localizes to the apicoplast. (2009)
  • 著者名/発表者名: Maeda T, Saito T, Harb OS, Roos DS, Takeo S, Suzuki H, Tsuboi T, Takeuchi T, Asai T.
  • 雑誌名: Parasitol. Int. 58 ページ: 101-105
  • NAID: 10025330344
  • 査読あり
• [雑誌論文] Rhoptry neck protein RON2 forms a complex with microneme protein AMA1 in Plasmodium falciparum merozoites. (2009)
  • 著者名/発表者名: Cao J, Kaneko O, Thongkukiatkul A, Tachibana M, Otsuki H, Gao Q, Tsuboi T, Torii M.
  • 雑誌名: Parasitol Int. 58 ページ: 29-35
  • NAID: 10025329950
  • 査読あり
• [雑誌論文] Single amino acid substitution in Plasmodium yoelii erythrocyte ligand determines its localization and controls parasite virulence (2009)
  • 著者名/発表者名: Otsuki H
  • 雑誌名: Proc Natl Acad Sci USA 106 ページ: 7167-7172
  • 査読あり
• [雑誌論文] A small-scale systematic analysis of alternative splicing in Plasmodium falciparum (2009)
  • 著者名/発表者名: Iriko H
  • 雑誌名: Parasitol Int 58 ページ: 196-199
  • 査読あり
• [雑誌論文] Wheat germ cell-free technology for accelerating the malaria vaccine research (2009)
  • 著者名/発表者名: Takeo S
  • 雑誌名: Expert Opin Drug Discov 4 ページ: 1191-1199
  • 査読あり
• [雑誌論文] Wheat germ cell-free system-based production of malaria proteins for discovery of novel vaccine candidates. (2008)
  • 著者名/発表者名: Tsuboi T, Takeo S, Iriko H, Jin L, Tsuchimochi M, Matsuda S, Han ET, Otsuki H, Kaneko O, Sattabongkot J, Udomsangpetch R, Sawasaki T, Torii M, Endo Y.
  • 雑誌名: Infect Immun. 76 ページ: 1702-1708
  • 査読あり
• [雑誌論文] Diversity and evolution of the rhoph1/clag multigene family of Plasmodium falciparum. (2008)
  • 著者名/発表者名: Iriko H, Kaneko O, Otsuki H, Tsuboi T, Su XZ, Tanabe K, Torii M.
  • 雑誌名: Mol Biochem Parasitol. 158 ページ: 11-21
  • 査読あり
• [雑誌論文] Diversity and evolution of the rhophl/clag multigene family of Plasmodium falciparum. (2008)
  • 著者名/発表者名: Iriko H
  • 雑誌名: Mol. Biochem. Parasitol. 158 ページ: 11-21
  • 査読あり
• [雑誌論文] Wheat germ cell-free system-based production of malaria proteins for discovery of novel vaccine candidates. (2008)
  • 著者名/発表者名: Tsuboi T
  • 雑誌名: Infect. Immun 76 ページ: 1702-1708
  • 査読あり
• [雑誌論文] Disruption of the Plasmodium berghei 2-Cys peroxiredoxin TPx-1 gene hinders the sporozoite development in the vector mosquito. (2008)
  • 著者名/発表者名: Yano K
  • 雑誌名: Mol. Biochem. Parasitol. 159 ページ: 142-145
  • 査読あり
• [雑誌論文] Detection of four Plasmodium species by genus-and species-specific loop-mediated isothermal amplification for clinical diagnosis. (2007)
  • 著者名/発表者名: Han ET, Watanabe R, Sattabongkot J, Khuntirat B, Sirichaisinthop J, Iriko H, Jin L, Takeo S, Tsuboi T.
  • 雑誌名: J Clin Microbiol. 45 ページ: 2521-2528
  • 査読あり
• [雑誌論文] Cell-free production of functional Plasmodium falciparum dihydrofolate reductase-thymidylate synthase. (2007)
  • 著者名/発表者名: Mudeppa DG, Pang CKT, Tsuboi T, Endo Y, Buckner FS, Varani G, Rathod PK.
  • 雑誌名: Mol Biochem Parasitol. 151 ページ: 216-219
  • 査読あり
• [雑誌論文] The Plasmodium falciparum RhopH2 promoter and first 24 amino acids are sufficient to target proteins to the rhoptries. (2007)
  • 著者名/発表者名: Ghoneim A, Kaneko O, Tsuboi T, Torii M.
  • 雑誌名: Parasitol Int. 56 ページ: 31-43
  • NAID: 10018566061
  • 査読あり
• [雑誌論文] Cell-free production of functional Plasmodium dihydrofolate reductase-thymidylate synthase (2007)
  • 著者名/発表者名: Mudeppa DG
  • 雑誌名: Mol. Biochem. Parasitol. 151 ページ: 216-219
  • 査読あり
• [雑誌論文] The Plasmodium falciparum RhopH2 promoter and first 24 amino acids are sufficient to target proteins to the rhoptries (2007)
  • 著者名/発表者名: Ghoneim A
  • 雑誌名: Parasitol. Int 56 ページ: 31-43
  • 査読あり
• [雑誌論文] Detection of four Plasmodium species by genus-and species-specific loop-mediated isothermal amplification for clinical diagnosis (2007)
  • 著者名/発表者名: Han ET
  • 雑誌名: J. Clin. Microbiol 45 ページ: 2521-2528
  • 査読あり
• [学会発表] Wheat germ cell-free protein synthesis system : a breakthrough for the post-genome malaria vaccine candidate discovery (2009)
  • 著者名/発表者名: Tsuboi T
  • 学会等名: Vivax malaria research III : 2009 and beyond
  • 発表場所: Gamboa, Panama
  • 年月日: 2009-05-25
• [学会発表] Identificationof novel blood-stage vaccine candidates against Plasmodium falciparum by high-throughput immunoscreening. (2009)
  • 著者名/発表者名: Takeo S.
  • 学会等名: ASTMH 58th annual meeting
  • 発表場所: Washington DC, USA
• [学会発表] Immunization with N-terminal region of a gametocyte protein Pfs230 successfully induce transmission-blocking antibodies against Plasmodium falciparum. (2009)
  • 著者名/発表者名: Tachibana M.
  • 学会等名: ASTMH 58th annual meeting
  • 発表場所: Washington DC, USA
• [学会発表] Discovering novel pre-erythrocytic antigens for malaria vaccines. (2009)
  • 著者名/発表者名: Aguiar JC.
  • 学会等名: ASTMH 58th annual meeting
  • 発表場所: Washington DC, USA
• [学会発表] Immunization with N-terminal region of a gametocyte protein Pfs230 successfully induce transmission-blocking antibodies against Plasmodium falciparum. (2009)
  • 著者名/発表者名: Tachibana M.
  • 学会等名: The 9th Awaji International Forum on Infection and Immunity
  • 発表場所: Awaji, Japan.
• [学会発表] Wheat germ cell-free protein synthesis system: a breakthrough for the post-genome malaria vaccine candidate discovery. (2009)
  • 著者名/発表者名: Tsuboi T.
  • 学会等名: Vivax malaria research III: 2009 and beyond, Gamboa, Panama
• [学会発表] fs230: Prefertilization Transmission-blocking Vaccine Candidate. (2009)
  • 著者名/発表者名: Tsuboi T.
  • 学会等名: Malaria Transmission Blocking Strategies. Bangkok
  • 発表場所: Thailand
• [学会発表] Wheat germ eel1-free system : A breakthrough in malaria vaccine candidate discovery. (2008)
  • 著者名/発表者名: Tsuboi T
  • 学会等名: 17th International Congress for Tropical Medicine and Malaria
  • 発表場所: Jeju Island, Korea
  • 年月日: 2008-10-02
• [学会発表] Wheat germ cell-free protein synthesis system: a key tool for novel malaria vaccine candidate discovery. (2008)
  • 著者名/発表者名: Tsuboi T.
  • 学会等名: Symposium on Capacity Building for Malaria Vaccine Development
  • 発表場所: Pune, India
  • 年月日: 2008-06-21
• [学会発表] Genome-wide malaria vaccine candidate discovery using wheat germ cell-free system. (2008)
  • 著者名/発表者名: Tsuboi T.
  • 学会等名: JSPS presents Sweden-Japan joint Seminar, Malaria Research - Diversity & Control
  • 発表場所: Stockholm, Sweden
  • 年月日: 2008-06-11
• [学会発表] Immunization with recombinant proteins of a gametocyte protein Pfs230 expressed using wheat germ cell-free system successfully induce transmission-blocking antibodies against Plasmodium falciparum. (2008)
  • 著者名/発表者名: Tachibana M.
  • 学会等名: ASTMH 57th annual meeting
  • 発表場所: New Orleans, USA
• [学会発表] Novel antigens at Plasmodium falciparum schizont-merozoite stages as potential vaccine candidates. (2008)
  • 著者名/発表者名: Takeo S.
  • 学会等名: ASTMH 57th annual meeting
  • 発表場所: New Orleans, USA
• [学会発表] A complex formation of rhoptry neck protein 2 with a microneme protein, AMA1, in Plasmodium falciparum. (2008)
  • 著者名/発表者名: Cao J.
  • 学会等名: ASTMH 57th annual meeting
  • 発表場所: New Orleans, USA
• [学会発表] Wheat germ cell-free system: A breakthrough in malaria vaccine candidate discovery. (2008)
  • 著者名/発表者名: Tsuboi T, Takeo S, Otsuki H, Torii M.
  • 学会等名: 17th International Congress for Tropical Medicine and Malaria
  • 発表場所: Jeju Island, Korea
• [学会発表] Erythrocyte- Binding-Like molecule and Virulence of Plasmodium yoelii. (2008)
  • 著者名/発表者名: Otsuki H.
  • 学会等名: 19th Annual Molecular Parasitology Meeting
  • 発表場所: Woods Hole, USA
• [学会発表] Novel antigens at Plasmodium falciparum schizont-merozoite stages as potential vaccine candidates. (2008)
  • 著者名/発表者名: Tsuboi T.
  • 学会等名: The 8th Awaji International Forum on Infection and Immunity
  • 発表場所: Awaji, Japan.
• [学会発表] Expression of malaria vaccine candidates using a wheat germ cell-free protein synthesis system without codon optimization. (2008)
  • 著者名/発表者名: Tsuboi T.
  • 学会等名: hird molecular Approaches to Malaria Meeting
  • 発表場所: Lorne, Australia
• [学会発表] Functional production of malaria proteins with wheat germ cell-free system. Keystone Symposia (2008)
  • 著者名/発表者名: Takeo S.
  • 学会等名: Structural Genomics and its Applications to Chemistry
  • 発表場所: Biology and Medicine, Steamboat Springs, USA
• [学会発表] Novel sporozoite antigen discovery of Plasmodium falciparum screened using human immunesera (2007)
  • 著者名/発表者名: Tsuboi T
  • 学会等名: The 7th Awaji International Forum on Infection and Immunity
  • 発表場所: Awaji,Hyogo,Japan
  • 年月日: 2007-09-05
• [学会発表] Novel sporozoite antigen discoverly of Plasmodium falciparum screened using human antisera. (2007)
  • 著者名/発表者名: Tsuboi T.
  • 学会等名: ASTMH 56th annual meeting
  • 発表場所: Philadelphia, USA
• [学会発表] Chitinase: active recombinant protein from Plasmodium vivax. (2007)
  • 著者名/発表者名: Takeo S.
  • 学会等名: ASTMH 56th annual meeting
  • 発表場所: Philadelphia, USA
• [学会発表] Plasmodium falciparum rhoptry neck protein (PfRON2) expressed at both erythrocytic and pre-erythrocytic invasive parasites. (2007)
  • 著者名/発表者名: Cao J.
  • 学会等名: The 7th Awaji International Forum on Infection and Immunity
  • 発表場所: Awaji, Japan.
• [学会発表] Novel sporozoite antigen discovery of Plasmodium falciparum screened using human immunesera (2007)
  • 著者名/発表者名: Tsuboi T.
  • 学会等名: The 7th Awaji International Forum on Infection and Immunity
  • 発表場所: Awaji, Japan.
• [学会発表] Transmission-blocking activity of DNA vaccine encoding Plasmodium vivax gametocyte protein Pvs230 (2007)
  • 著者名/発表者名: Tachibana M.
  • 学会等名: ASTMH 56th annual meeting
  • 発表場所: Philadelphia, USA
• [備考]
  • URL: http://www.ehime-u.ac.jp/~cellfree/proteomedical/malaria.html
• [備考]
  • URL: http://www.ehime-u.ac.jp/~cellfree/proteomedical/malaria.html
• [備考]
  • URL: http://www.ehime-u.ac.jp/~cellfree/proteomedical/malaria.html