メダカ性分化における細胞増殖因子の役割

• 研究課題/領域番号: 19770051
• 研究種目: 若手研究(B)
• 配分区分: 補助金
• 研究分野: 形態・構造
• 研究機関: 熊本大学
• 研究代表者: 北野 健 熊本大学, 大学院・自然科学研究科, 准教授 (40336219)
• 研究期間 (年度): 2007 – 2008
• 研究課題ステータス: 完了 (2008年度)
• 配分額: 3,690千円 (直接経費: 3,300千円、間接経費: 390千円); 2008年度: 1,690千円 (直接経費: 1,300千円、間接経費: 390千円); 2007年度: 2,000千円 (直接経費: 2,000千円)
• キーワード: メダカ / 性分化 / 生殖細胞

研究概要

メダカ性分化において、増殖因子であるMIS(Mullerian inhibiting substance)の生理的機能を明らかにするため、アンチセンスオリゴを用いたMIS及びMIS受容体の機能阻害実験を行った。その結果、MISまたはMIS受容体の機能阻害により、雌雄の生殖細胞の増殖が完全に抑制された。このことから、MISは、メダカ性分化時期において、雌雄の生殖細胞の増殖に不可欠な因子であることが明らかとなった。

研究成果

• [雑誌論文] Mullerian inhibiting substance is required for germ cell proliferation during early gonadal differentiation in medaka (Oryzias latipies) (2008)
  • 著者名/発表者名: Shiraishi E., Yoshinaga N., Miura T., Yokoi H., Wakamatsu Y., Abe S. and Kitano T.
  • 雑誌名: Endocrinology 149 ページ: 1813-1819
  • 査読あり
• [雑誌論文] Cyp11b1 is induced in the murine gonad by luteinizing hormone/human chorionic gonadotropin and involved in the production of 11-ketotestosterone, a major fish androgen ; conservation and evolution of androgen metabolic pathway. (2008)
  • 著者名/発表者名: Yazawa T., Uesaka M., Inaoka Y., Mizutani T., Sekiguchi T., Kajitani T., Kitano T., Umezawa A. and Miyamoto K.
  • 雑誌名: Endocrinology 149 ページ: 1786-1792
  • 査読あり
• [雑誌論文] Mullerian inhibiting substance is required for germ cell proliferation during early gonadal differentiation in medaka (2008)
  • 著者名/発表者名: Shiraishi E.
  • 雑誌名: Endocrinology 149(Oryzias latipies) ページ: 1813-1819
  • 査読あり
• [雑誌論文] Cypllb1 is induced in the murine gonad by luteinizing hormone/ human chorionic gonadotropin and involved in the production of 11-ketotestosterone, a major fish androgen ; conservation and evolution of androgen metabolic nathway (2008)
  • 著者名/発表者名: Yazawa T.
  • 雑誌名: Endocrinology 149 ページ: 1786-1792
  • 査読あり
• [雑誌論文] Tributyltin-binding protein type 1 has a distinctive lipocalin-like structure and is involved in the excretion of tributyltin in Japanese flounder, Paralichthys olivaceus (2008)
  • 著者名/発表者名: Satone H.
  • 雑誌名: Aquatic Toxicology 90 ページ: 229-238
  • 査読あり
• [雑誌論文] The mechanism of transcriptional regulation of Mullerian inhibiting substance in Japanese flounder (2008)
  • 著者名/発表者名: Shirozu T.
  • 雑誌名: Cybium 32(sunpl)(Paralichthvs olivaceus) ページ: 90-91
  • 査読あり
• [雑誌論文] The mechanism of transcriptional regulation of P450 aromatase gene by. Fox12 in Japanese flounder (2008)
  • 著者名/発表者名: Yamaguchi T.
  • 雑誌名: Cybium 32 (suppl)(Paralichthys olivaceus) ページ: 74-76
  • 査読あり
• [雑誌論文] Strain difference of cadmium accumulation by liver slices of inbred Wistar-Imamichi and Fischer 344 rats. (2008)
  • 著者名/発表者名: Shimada H.
  • 雑誌名: Toxicology in Vitro 22 ページ: 338-343
• [雑誌論文] Size-selective junctional barrier and Ca2+independent cell adhesion in the testis of Cynops pyrrhogaster: expression and function of occludin. (2008)
  • 著者名/発表者名: Jin Y.
  • 雑誌名: Molecular Reproduction and Development 75 ページ: 202-216
• [雑誌論文] Fish Reproduction (Involvement of endocrine and environmental factors (2008)
  • 著者名/発表者名: Kitano T.
  • 雑誌名: Fish Reproduction (Involvement of endocrine and environmental factors in gonadal sex differentiation in gonochoristic fish.), Science Publishers ページ: 421-434
• [雑誌論文] Follicle-stimulating hormone signaling and Fox12 are involved in transcriptional regulation of aromatase gene during gonadal sex differentiation in Japanese flounder, Paralichthys olivaceus. (2007)
  • 著者名/発表者名: Yamaguchi T., Yamaguchi S., Hirai T. and Kitano T.
  • 雑誌名: Biochem. Biophys. Res. Commun 359 ページ: 935-940
  • 査読あり
• [雑誌論文] Tamoxifen induces masculinization of genetic females and regulates P450 aromatase and Mullerian inhibiting substance mRNA expression in Japanese flounder (Paralichthys olivaceus). (2007)
  • 著者名/発表者名: Kitano T., Yoshinaga N., Shiraishi E., Koyanagi T. and Abe S.
  • 雑誌名: Mol. Reprod. Dev 74 ページ: 1171-1177
  • 査読あり
• [雑誌論文] Tamoxifen induces masculinization of genetic females and regulates P450 aromatase and Mullerian inhibiting substance mRNA expression in Japanese flounder(Paralichthys olivaceus). (2007)
  • 著者名/発表者名: Kitano T.
  • 雑誌名: Molecular Reproduction and Development 74 ページ: 1171-1177
• [雑誌論文] Follicle-Stimulating hormone signaling and Foxl2 are involved in transcriptional regulation of aromatase gene during gonadal sexdifferentiation in Japanese flounder, Paralichthys olivaceus. (2007)
  • 著者名/発表者名: Yamaguchi T.
  • 雑誌名: Biochemical and Biophysical Research Communications 359 ページ: 935-940
• [雑誌論文] Purification and characterization of tributyltin binding protein type 2 from plasma of Japanese flounder. Paralichthys olivaceus. (2007)
  • 著者名/発表者名: Oba Y.
  • 雑誌名: Journal of Biochemistry 142 ページ: 229-238
• [雑誌論文] Tributlyltin causes abnormal development in embryo of medaka. Oryzias latipes. (2007)
  • 著者名/発表者名: Hano T.
  • 雑誌名: Chemosphere 69 ページ: 927-933
• [学会発表] Toxicogenomics and sexual differentiation in fish (2008)
  • 著者名/発表者名: Kitano T (Invited)
  • 学会等名: 4th International Conference on Toxicogenomics
  • 発表場所: Incheon, Korea
• [学会発表] The mechanism of transcriptional regulation of P450 aromatase gene by Fox12 in Japanese flounder(Paralichthys olivaceus). (2007)
  • 著者名/発表者名: Yamaguchi T.
  • 学会等名: 8th International Symposium on Reproductive Physiology of Fish
  • 発表場所: st Malo, France
  • 年月日: 2007-06-05
• [学会発表] Role of Mullerian inhibiting substance on gonadal sex differentiation in medaka (Oryzias latipes) (2007)
  • 著者名/発表者名: Shiraishi E., Miura T., Abe S. and Kitano T.
  • 学会等名: 8th International Symposium on Reproductive Physiology of Fish
  • 発表場所: St Malo, France
• [学会発表] The mechanism of transcriptional regulation of Mullerian inhibiting substance in Japanese flounder (Paralichthys olivaceus) (2007)
  • 著者名/発表者名: Shirozu, T., Shiraishi E., Yoshinaga N., Kanamori A., Kubo Y., Hori H. and Kitano T.
  • 学会等名: 8th International Symposium on Reproductive Physiology of Fish
  • 発表場所: St Malo, France
• [学会発表] The mechanism of transcriptional regulation of P450 aromatase gene by Fox12 in Japanese flounder (Paralichthys olivaceus) (2007)
  • 著者名/発表者名: Yamaguchi T. and Kitano T.
  • 学会等名: 8th International Symposium on Reproductive Physiology of Fish
  • 発表場所: StMalo, France
• [図書] Fish Reproduction (Involvement of endocrine and environmental factors in gonadal sex differentiation in gonochoristic fish.) (2008)
  • 著者名/発表者名: Kitano T.
  • 出版者: Science Publishers
• [図書] Fish Reproduction (2007)
  • 著者名/発表者名: Kitano T. and Abe S.-I.
  • 出版者: Involvement of endocrine and environmental factors in gonadal sex differentiation in fish