転写因子E2F1を軸としたフィードバック・ループによる細胞機能制御の解明

• 研究課題/領域番号: 19770179
• 研究種目: 若手研究(B)
• 配分区分: 補助金
• 研究分野: 細胞生物学
• 研究機関: 明治大学
• 研究代表者: 吉田 健一 明治大学, 農学部, 准教授 (20345036)
• 研究期間 (年度): 2007 – 2008
• 研究課題ステータス: 完了 (2008年度)
• 配分額: 3,870千円 (直接経費: 3,300千円、間接経費: 570千円); 2008年度: 2,470千円 (直接経費: 1,900千円、間接経費: 570千円); 2007年度: 1,400千円 (直接経費: 1,400千円)
• キーワード: 遺伝子 / シグナル伝達 / 転写因子 / 細胞周期 / プロモーター / 分子生物学

研究概要

研究では「細胞周期とアポトーシスを制御する転写因子E2F1の転写ネットワークの理解」を目的として、E2F1を軸としたフィードバック・ループによる細胞機能制御の解明を目指した。特本に、E2F1の新規ヒト標的遺伝子として見出したGemininならびにCDCA4について解析を進めた。E2F1-CDCA4、E2F1-Gemininといったフィードバック・ループによりE2F1活性が制御される可能性が示唆され、E2F1を介した細胞増殖・分化や細胞死などの制御を考える上で重要な知見であると推察される。

研究成果

• [雑誌論文] Hyperosmotic stress up-regulates the expression of major vault protein in SW620 human colon cancer cells (2008)
  • 著者名/発表者名: Ikeda R, Iwashita K, Sumizawa T, Beppu S, Tabata S, Tajitsu Y, Shimamoto Y, Yoshida K, Furukawa T, Che X, Yamaguchi T, Ushiyama M, Miyawaki A, Takeda Y, Yamamoto M, Zhao H, Shibayama Y, Yamada K, Akiyama S
  • 雑誌名: Experimental Cell Research 314 ページ: 3017-3026
  • 査読あり
• [雑誌論文] Transcriptional co-factor CDCA4 participates in the regulation of JUN oncogene expression (2008)
  • 著者名/発表者名: Tategu M, Nakagawa H, Hayashi R, Yoshida K
  • 雑誌名: Biochimie 90 ページ: 1515-1522
  • 査読あり
• [雑誌論文] Thymidine phosphorylase inhibits the expression of proapoptic protein BNIP3 (2008)
  • 著者名/発表者名: Ikeda R, Tajitsu Y, Iwashita KI, Che XF, Yoshida K, Ushiyama M, Furukawa T, Komatsu M, Yamaguchi T, Shibayama Y, Yamamoto M, Zhao HY, Arima J, Takeda Y, Akiyama SI, Yamada K
  • 雑誌名: Biochemical and Biophysical Research Communications 370 ページ: 220-224
  • 査読あり
• [雑誌論文] Transcriptional regulation of an evolutionary conserved intergenic region of CDT2-INTS7 (2008)
  • 著者名/発表者名: Nakagawa H, Tategu M, Yamauchi R, Sasaki K, Sekimachi S, Yoshida K
  • 雑誌名: PLoS ONE 3(1):e1484 ページ: 1-8
  • 査読あり
• [雑誌論文] Puma is a novel target of soy isoflavone genistein but is dispensable for genistein-induced cell fate determination (2008)
  • 著者名/発表者名: Tategu M, Arauchi T, Tanaka R, Nakagawa H, Yoshida K
  • 雑誌名: Molecular Nutrition and Food Research 52 ページ: 439-446
  • 査読あり
• [雑誌論文] Transcriptional regulation of polo-like kinases and early mitotic inhibitors (2008)
  • 著者名/発表者名: Tategu M, Nakagawa H, Sasaki K, Yamauchi R, Sekimachi S, Suita Y, Watanabe N, Yoshida K
  • 雑誌名: Journal of Genetics and Genomics 35 ページ: 215-224
  • 査読あり
• [雑誌論文] Hyperosmotic stress up-regulates the expression of major vault protein in SW620 human colon cancer cells (2008)
  • 著者名/発表者名: Ikeda R, et.al.
  • 雑誌名: Experimenta Cell Research 314 ページ: 3017-3026
  • 査読あり
• [雑誌論文] Transcriptional co-factor CDCA4 participates in the regulation of JUN oncogene expression (2008)
  • 著者名/発表者名: Tategu M, et.al.
  • 雑誌名: Biochimie 90 ページ: 1515-1522
  • 査読あり
• [雑誌論文] Thymidine phosphorylase inhibits the expression of proapoptic protein BNIP3 (2008)
  • 著者名/発表者名: Ikeda R, et.al.
  • 雑誌名: Biochemical and Biophysical Research Communications 370 ページ: 220-224
  • 査読あり
• [雑誌論文] Transcriptional regulation of an evolutionary conserved intergenic region of CDT2-INTS7 (2008)
  • 著者名/発表者名: Nakagawa H, et.al.
  • 雑誌名: PLoS ONE 3
  • 査読あり
• [雑誌論文] Puma is a novel target of soy isoflavone genistein but is dispensable for genistein-induced cell fate determination (2008)
  • 著者名/発表者名: Tategu M, et.al.
  • 雑誌名: Molecular Nutrition and Food Research 52 ページ: 439-446
  • 査読あり
• [雑誌論文] Transcriptional regulation of polo-like kinases and early mitotic inhibitors (2008)
  • 著者名/発表者名: Tategu M, et.al.
  • 雑誌名: Journal of Genetics and Genomics 35 ページ: 215-224
  • 査読あり
• [雑誌論文] Transcriptional regulation of an evolutionary conserved intergenic region of CDT2-INTS7 (2008)
  • 著者名/発表者名: Nakagawa H et al
  • 雑誌名: PLoS ONE 3
  • 査読あり
• [雑誌論文] Transcriptional regulation of human chromatin assembly factor ASF1 (2007)
  • 著者名/発表者名: Hayashi R, Goto Y, Tanaka R, Oonogi K, Hisasue M, Yoshida K
  • 雑誌名: DNA and Cell Biology 26 ページ: 91-96
  • 査読あり
• [雑誌論文] Identification of FAZF as a novel BMP2-induced transcription factor during osteoblastic differentiation (2007)
  • 著者名/発表者名: Ikeda R, Yoshida K, Inoue I
  • 雑誌名: Journal of Cellular Biochemistry 101 ページ: 174-154
  • 査読あり
• [雑誌論文] Systems biology-based identification of crosstalk between E2F transcription factors and the Fanconi anemia pathway (2007)
  • 著者名/発表者名: Tategu M, Arauchi T, Tanaka R, Nakagawa H, Yoshida K
  • 雑誌名: Gene Regulation and Systems Biology 1 ページ: 1-8
  • 査読あり
• [雑誌論文] Gene expression changes during the chondrogenic differentiation of human mesenchymal stem cells (2007)
  • 著者名/発表者名: Ikeda R, Tsukahara S, Yoshida K, Inoue I
  • 雑誌名: Journal of Biological Sciences 7 ページ: 729-736
  • 査読あり
• [雑誌論文] Identification of FAZF as a novel BMP2-induced transcription factor during osteoblastic differentiation (2007)
  • 著者名/発表者名: Ikeda R et al
  • 雑誌名: Journal of Cellular Biochemistry 101 ページ: 147-154
  • 査読あり
• [雑誌論文] Gene expression changes induced by TGF-betal, IGF-1, and insulin during the chondrogenic differentiation of human mesenchymal stemcells (2007)
  • 著者名/発表者名: Ikeda R et al
  • 雑誌名: Journal of Biological Sciences 7 ページ: 729-736
  • 査読あり
• [学会発表] Human microRNAs regulated by cancer signaling pathways (2009)
  • 著者名/発表者名: Yoshida K
  • 学会等名: The Fifth Asia Pacific Multidisciplinary Meeting for Cancer Research, The Annual Scientific Meeting for Cancer Research Cancer 2009 "microRNA in Cancer Development"
  • 発表場所: Prince of Wales Hospital, Hong Kong, China
• [学会発表] Human microRNAs regulated by cancer signaling pathways (2009)
  • 著者名/発表者名: Yoshida K
  • 学会等名: The Fifth Asia Pacific Multidisciplinary Meeting for Cancer Research
  • 発表場所: Hong Kong, China
• [学会発表] Promoter evolution of MCM8 orthologous gene in mammalian genome (2008)
  • 著者名/発表者名: Yoshida K
  • 学会等名: 22nd International Mammalian Genome Conference
  • 発表場所: Institute of Molecular Genetics, Prague, Czech Republic
• [学会発表] Regulation of transcription factor E2F1 activity by TRIP-Br family protein CDCA4 (2008)
  • 著者名/発表者名: Tategu M, Yoshida K
  • 学会等名: The 9th International Congress on Cell Biology
  • 発表場所: COEX, Seoul, Korea
• [学会発表] E2F1 regulates mitochondrial biogenesis possibly through mitochondrial topoisomerase I (2008)
  • 著者名/発表者名: Nakagawa H, Yoshida K
  • 学会等名: The 9th International Congress on Cell Biology
  • 発表場所: COEX, Seoul, Korea
• [学会発表] Phage display screening of Geminin-binding peptide and validation of its therapeutic use in tumors (2008)
  • 著者名/発表者名: Yoshida K
  • 学会等名: 30th European Peptide Symposium
  • 発表場所: Finlandia Hall, Helsinki, Finland
• [学会発表] Comprehensive analysis of cell-cycle-associated biomarkers induced by genistein (2008)
  • 著者名/発表者名: Yoshida K
  • 学会等名: HUPO(Human Proteome Organisation) 7th World Congress
  • 発表場所: RAI International Convention Center, Amsterdam, Netherlands
• [学会発表] Gene regulatory link between the Fanconi anemia/BRCA pathway and E2F transcription factors (2008)
  • 著者名/発表者名: Yoshida K
  • 学会等名: ISCO(International Society for Cellular Oncology) 2008
  • 発表場所: Vrije Universiteit, Amsterdam, Netherlands
• [学会発表] Nutriproteomics approach to interpret the biological effect of soybean isoflavone in cell fate determination (2008)
  • 著者名/発表者名: Yoshida K
  • 学会等名: Nutrigenomics 2008
  • 発表場所: Faculte de Medecine, Universite Paris V, Paris, France
• [学会発表] Comprehensive analysis of cell-cycle-associated biomarkers induced by genistein (2008)
  • 著者名/発表者名: Yoshida K
  • 学会等名: HUPO (Human Proteome Organisation) 7^<th> world Congress
  • 発表場所: RAI International Con vention Center, Amste rdam, Netherlands
• [学会発表] Phage display screening of Geminin-binding peptide and va lidation of its therapeutic use in tumors (2008)
  • 著者名/発表者名: Yoshida K
  • 学会等名: 30^<th> European Peptide Symposium
  • 発表場所: Helsinki, Finland
• [学会発表] E2F1 regulates mitochondrial biogenesis possibly through mitochondrial topoisomerase I (2008)
  • 著者名/発表者名: Nakagawa H
  • 学会等名: The 9^<th> International Congress on Cell Biology
  • 発表場所: COEX, Seoul, Korea
• [学会発表] Regulation of transcription factor E2F1 activity by TRIP-Br family protein CDCA4 (2008)
  • 著者名/発表者名: Tategu M
  • 学会等名: The 9^<th> International Congress on Cell Biology
  • 発表場所: COEX, Seoul, Korea
• [学会発表] Promoter evolution of MCM8 orthologous gene in mammalian genome (2008)
  • 著者名/発表者名: Yoshida K
  • 学会等名: 22nd International Mammalian Genome Conference
  • 発表場所: Prague, Czech Republic
• [学会発表] Genetic approach to dissect the molecular mechanism of cell-cycle-associated genes in human cells (2007)
  • 著者名/発表者名: Yoshida K
  • 学会等名: 12th World Congress on Advances in Oncology and 10th International Symposium on Molecular Medicine
  • 発表場所: Creta Maris Hotel, Hersonissos, Greece
• [学会発表] Involvement of transcription factor E2F1 in nucleo-mitochondrial crosstalk (2007)
  • 著者名/発表者名: Yoshida K
  • 学会等名: 32nd Annual FEBS Congress "Molecular Machines"
  • 発表場所: MessezentrumWienNeu, Vienna, Austria
• [学会発表] Evolutionary conserved CDT2-INTS7 intergenic region acts as a bidirectional promoter and is asymmetrically regulated (2007)
  • 著者名/発表者名: Yoshida K
  • 学会等名: 21st International Mammalian Genome Conference
  • 発表場所: Kyoto Terrsa, Kyoto
• [学会発表] Involvement of transcription factor E2F1 in nucleo-mitochondrial crosstalk (2007)
  • 著者名/発表者名: Yoshida K
  • 学会等名: 32nd Annual FEBS Congress
  • 発表場所: Vienna, Austria
• [図書] In : Control of Cellular Physiology by Transcription Factors E2F. Ed. Yoshida K. (435 pages, Research Signpost, Kerala) (2008)
  • 著者名/発表者名: Yoshida K
  • 出版者: Spatiotemporal dynamics of E2F transcription factors : Coordinated action but in a characteristic manner
• [図書] In : Trends in Cell Cycle Research. Ed. Yoshida K. (317 pages, Research Signpost, Kerala) (2008)
  • 著者名/発表者名: Yoshida K
  • 出版者: Hidden treasures of G1/S cyclins/CDKs/pRb/E2F pathway
• [図書] In : Protein Conformation : New Research. Ed. Roswell LB. Chapter5(264 pages, Nova Science Publishers, New York) (2008)
  • 著者名/発表者名: Yoshida K
  • 出版者: Hierarchical order of initiator protein assembly in pre-replication complex
• [図書] In : Cell Cycle Control : New Research. Ed. Leroy NH and Fournier NT. (496 pages, Nova Science Publishers, New York) (2008)
  • 著者名/発表者名: Yoshida K
  • 出版者: Regulation of cell fate determination by Jun, a component of activator protein 1
• [図書] In : Cell Cycle Control : New Research. Ed. Leroy NH and Fournier NT. (496 pages, Nova Science Publishers, New York) (2008)
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  • 出版者: The beginning of the microRNAs era for cell cycle control
• [図書] Control of Cellular Physiology by Transcription Factors E2F (2008)
  • 著者名/発表者名: Yoshida K
  • 総ページ数: 435
  • 出版者: Research Signpost
• [図書] Trends in Cell Cycle Research (2008)
  • 著者名/発表者名: Yoshida K
  • 総ページ数: 317
  • 出版者: Research Signpost
• [図書] Protein Conformation: New Research(Ed. Roswell LB) (2008)
  • 著者名/発表者名: Yoshida K
  • 出版者: Nova Science Publishers(in press)