大脳辺縁系および脳幹における急性ストレス反応

• 研究課題/領域番号: 19890189
• 研究種目: 若手研究(スタートアップ)
• 配分区分: 補助金
• 研究分野: 法医学
• 研究機関: 大阪市立大学
• 研究代表者: 権 力 大阪市立大学, 大学院・医学研究科, 助教 (60453130)
• 研究期間 (年度): 2007 – 2009
• 研究課題ステータス: 完了 (2008年度)
• 配分額: 3,039千円 (直接経費: 2,640千円、間接経費: 399千円); 2008年度: 1,729千円 (直接経費: 1,330千円、間接経費: 399千円); 2007年度: 1,310千円 (直接経費: 1,310千円)
• キーワード: ストレス反応 / 大脳辺縁系 / 脳幹 / 神経病理 / 神経化学 / エリスローポエチン / C反応性蛋白 / 受傷後生存時間 / ドーパミン / セロトニン / ノルアドレナリン

研究概要

法医鑑定では, 犯罪被害者の死因のみならず, 生存時間や受傷時の苦痛・身体的行動能力などについての医学的立証が求められることがある.ヒトの死の直前における神経伝達物質の中, 特にカテコラミンの測定を中心として, 致死的外傷(侵害刺激)が中枢神経系に及ぼす急性ストレス反応を生化学的に評価し, ヒト(法医剖検例)における脳組織, 脳脊髄液および血液内に残された痕跡を分子病理学, 分子生物学および神経化学・生理学的指標を用いて多元多角的に分析した.

研究成果

• [雑誌論文] Analyses of cardiac blood cells and serum proteins with regard to cause of death in forensic autopsy cases (2009)
  • 著者名/発表者名: Quan L, Ishikawa T, Michiue T, Li DR, Zhao D, Yoshida C, Chen JH, Komatsu A, Azuma Y, Sakoda S, Zhu BL, Maeda H.
  • 雑誌名: Leg Med (Tokyo) (Epub ahead of print)
  • NAID: 10027262983
• [雑誌論文] Postmortem serum levels of pulmonary surfactant-associated proteins A and D with regard to the cause of death in medicolegal autopsy (2009)
  • 著者名/発表者名: Quan L, Zhu BL, Ishikawa T, Michiue T, Zhao D, Yoshida C, Chen JH, Wang Q, Komatsu A, Azuma Y, Maeda H.
  • 雑誌名: Leg Med (Tokyo) (Epub ahead of print)
  • NAID: 10027262989
• [雑誌論文] Immunohistochemistry of von Willebrand factor in the lungs with regard to the cause of death in forensic autopsy (2009)
  • 著者名/発表者名: Quan L, Ishikawa T, Zhao D, Michiue T, Yoshida C, Chen JH, Wang Q, Zhu BL, Maeda H.
  • 雑誌名: Leg Med (Tokyo) (Epub ahead of print)
  • NAID: 10027262975
• [雑誌論文] Quantitative analysis of single-stranded DNA immunoreactivity as a marker of neuronal apoptosis in hippocampus with regard to the causes of death in medicolegal autopsy (2009)
  • 著者名/発表者名: Michiue T, Quan L, Ishikawa T, Zhu BL, Maeda H.
  • 雑誌名: Leg Med(Tokyo) (Epub ahead of print)
  • NAID: 10027262572
• [雑誌論文] Evaluation of postmortem calcium and magnesium levels in the pericardial fluid with regard to the cause of death in medicolegal autopsy (2009)
  • 著者名/発表者名: Li DR, Quan L, Zhu BL, Ishikawa T, Michiue T, Zhao D, Yoshida C, Chen JH, Wang Q, Komatsu A, Azuma Y, Maeda H.
  • 雑誌名: Leg Med (Tokyo) (Epub ahead of print)
  • NAID: 10027262910
• [雑誌論文] Postmortem mRNA quantification for investigation of infantile death : A comparison with adult cases (2009)
  • 著者名/発表者名: Zhao D, Ishikawa T, Quan L, Michiue T, Yoshida C, Komatu A, Chen JH, Wang Q,Zhu BL, Maeda H.
  • 雑誌名: Leg Med (Tokyo) (Epub ahead of print)
  • NAID: 10027262946
• [雑誌論文] Postmortem quantitative mRNA analyses of death investigation in forensic pathology : An overview and prospects (2009)
  • 著者名/発表者名: Zhao D, Ishikawa T, Quan L, Michiue T, Zhu BL, Maeda H.
  • 雑誌名: Leg Med (Tokyo) [Epub ahead of print]
  • NAID: 10027261968
• [雑誌論文] Evaluation of pulmonary GLUT1 and VEGF mRNA levels in relation to lung weight in medicolegal autopsy cases (2009)
  • 著者名/発表者名: Zhao D, Ishikawa T, Quan L, Michiue T, Yoshida C, Komatu A, Chen JH, Wang Q, Zhu BL, Maeda H.
  • 雑誌名: Leg Med [Epub ahead of print]
  • NAID: 10027262961
• [雑誌論文] Immunohistochemical distribution of basic fibroblast growth factor (bFGF) in medicolegal autopsy (2009)
  • 著者名/発表者名: Wang Q, Ishikawa T, Quan L, Zhao D, Li DR, Michiue T, Chen JH, Zhu BL, Maeda H.
  • 雑誌名: Leg Med (Tokyo) [Epub ahead of print]
  • NAID: 10027262517
• [雑誌論文] Significance of postmortem biochemistry in determining the cause of death (2009)
  • 著者名/発表者名: Maeda H, Zhu BL, Ishikawa T, Quan L, Michiue T.
  • 雑誌名: Leg Med [Epub ahead of print]
  • NAID: 10027261990
• [雑誌論文] Analysis of postmortem biochemical findings with regard to the lung weight in drowning (2009)
  • 著者名/発表者名: Maeda H, Zhu BL, Ishikawa T, Quan L, Michiue T, Bessho Y, Okazaki S, Kamikodai Y, Tsuda K, Komatsu A, Azuma Y.
  • 雑誌名: Leg Med [Epub ahead of print]
  • NAID: 10027262889
• [雑誌論文] Analyses of cardiac blood cells and serum proteins with regard to cause of death in forensic autopsy cases. (2009)
  • 著者名/発表者名: Quan L, Ishikawa T, Michiue T, Li DR, Zhao D, Yoshida C, Chen JH, Komatsu A, Azuma Y, Sakoda S, Zhu BL, Maeda H.
  • 雑誌名: Legal Med. (In press)
  • NAID: 10027262983
  • 査読あり
• [雑誌論文] Postmortem serum levels of pulmonary surfactant-associated proteins A and D with regard to the cause of death in medicolegal autopsy. (2009)
  • 著者名/発表者名: Quan L, Zhu BL, Ishikawa T, Michiue T, Zhao D, Yoshida C, Chen JH, Wang Q, Komatsu A, Azuma Y, Maeda H.
  • 雑誌名: Legal Med. (In press)
  • NAID: 10027262989
  • 査読あり
• [雑誌論文] Immunohistochemistry of von Willebrand factor in the lungs with regard to the cause of death in forensic autopsy. (2009)
  • 著者名/発表者名: Quan L, Ishikawa T, Zhao D, Michiue T, Yoshida C, Chen JH, Wang Q, Zhu BL, Maeda H.
  • 雑誌名: Legal Med. (In press)
  • NAID: 10027262975
  • 査読あり
• [雑誌論文] Quantitative analysis of single-stranded DNA immunoreactivity as a marker of neuronal apoptosis in hippocampus with regard to the causes of death in medicolegal autopsy. (2009)
  • 著者名/発表者名: Michiue T, Quan L, Ishikawa T, Zhu BL, Maeda H.
  • 雑誌名: Legal Med. (In press)
  • NAID: 10027262572
  • 査読あり
• [雑誌論文] Evaluation of postmortem calcium and magnesium levels in the pericardial fluid with regard to the cause of death in medicolegal autopsy. (2009)
  • 著者名/発表者名: Li DR, Quan L, Zhu BL, Ishikawa T, Michiue T, Zhao D, Yoshida C, Chen JH, Wang Q, Komatsu A, Azuma Y, Maeda H.
  • 雑誌名: Legal Med. (In press)
  • NAID: 10027262910
  • 査読あり
• [雑誌論文] Evaluation of pulmonary GLUT1 and VEGF mRNA levels in relation to lung weight in medicolegal autopsy cases. (2009)
  • 著者名/発表者名: Zhao D, Ishikawa T, Quan L, Michiue T, Yoshida C, Komatu A, Chen JH, Wang Q, Zhu BL, Maeda H.
  • 雑誌名: Legal Med. (In press)
  • NAID: 10027262961
  • 査読あり
• [雑誌論文] Postmortem serum erythropoietin levels in establishing the cause of death and survival time at medicolegal autopsy (2008)
  • 著者名/発表者名: Quan L, Zhu BL, Ishikawa T, Michiue T, Zhao D, Li DR, Ogawa M, Maeda H.
  • 雑誌名: Int J Legal Med 122(6) ページ: 481-7
  • 査読あり
• [雑誌論文] Postmortem biochemistry and immunohistochemistry of adrenocorticotropic hormone with special regard to fatal hypothermia (2008)
  • 著者名/発表者名: Ishikawa T, Quan L, Li DR, Zhao D, Michiue T, Hamel M, Maeda H.
  • 雑誌名: Forensic Sci Int 179(2-3) ページ: 147-51
  • 査読あり
• [雑誌論文] Single-stranded DNA as an immunohistochemical marker of neuronal damage in human brain : an analysis of autopsy material with regard to the cause of death (2008)
  • 著者名/発表者名: Michiue T, Ishikawa T, Quan L, Li DR, Zhao D, Komatsu A, Zhu BL, Maeda H.
  • 雑誌名: Forensic Sci Int 178(2-3) ページ: 185-91
  • 査読あり
• [雑誌論文] Suicidal vehicle-assisted ligature strangulation resulting in complete decapitation : Anautopsy report and a review of the literature (2008)
  • 著者名/発表者名: Zhao D, Ishikawa T, Quan L, Li DR, Michiue T, Maeda H.
  • 雑誌名: Leg Med 10(6) ページ: 310-5
  • 査読あり
• [雑誌論文] Immunohistochemical distribution of single-stranded DNA in the brain in medico-legal autopsy cases of carbon monoxide intoxication (2008)
  • 著者名/発表者名: Michiue T, Ishikawa T, Quan L, Li DR, Komatsu A, Zhao D, Chen JH, Yoshida C, Ogawa M, Maeda H.
  • 雑誌名: Chudoku Kenkyu 21(1) ページ: 63-8
  • 査読あり
• [雑誌論文] Tissue-specific differences in mRNA quantification of glucose transporter 1 and vascular endothelial growth factor with special regard to death investigations of fatal injuries (2008)
  • 著者名/発表者名: Zhao D, Ishikawa T, Quan L, Li DR, Michiue T, Yoshida C, Komatu A, Chen JH, Zhu BL, Maeda H.
  • 雑誌名: Forensic Sci Int 177(2-3) ページ: 176-83
  • 査読あり
• [雑誌論文] Forensic pathological evaluation of injury severity and fatal outcome in traffic accidents : five illustrative autopsy cases of clinically unexpected death (2008)
  • 著者名/発表者名: Michiue T, Ishikawa T, Quan L, Zhu BL, Maeda H.
  • 雑誌名: Forensic Sci Med Pathol 4(3) ページ: 153-8
  • 査読あり
• [雑誌論文] 片側性血胸に合併した対側性外傷性気胸による急死の1剖検例 (2008)
  • 著者名/発表者名: 権力, 道上知美, 趙東, 李冬日, 石川隆紀, 前田均
  • 雑誌名: 法医病理 (14) ページ: 22-28
  • 査読あり
• [雑誌論文] Postmortem serum erythropoietin levels in establishing the cause of death and survival time at medicolegal autopsy. (2008)
  • 著者名/発表者名: Quan L, Zhu BL, Ishikawa T, Michiue T, Zhao D, Li DR, Ogawa M, Maeda H.
  • 雑誌名: Int J Legal Med. 122 ページ: 481-487
  • 査読あり
• [雑誌論文] Postmortem biochemistry and immunohistochemistry of adrenocort icotropic hormone with special regard to fatal hypothermia. (2008)
  • 著者名/発表者名: Ishikawa T, Quan L, Li DR, Zhao D, Michiue T, Hamel M, Maeda H.
  • 雑誌名: Forensic Sci Int. 179 ページ: 147-151
  • 査読あり
• [雑誌論文] Single-stranded DNA as an immunohistochemical marker of neuronal damage in human brain : an analysis of autopsy material with regard to the cause of death. (2008)
  • 著者名/発表者名: Michiue T, Ishikawa T, Quan L, Li DR, Zhao D, Komatsu A, Zhu BL, Maeda H.
  • 雑誌名: Forensic Sci Int. 178 ページ: 185-191
  • 査読あり
• [雑誌論文] Suicidal vehicle-assisted ligature strangulation resulting in complete decapitation : An autopsy report and a review of the literature. (2008)
  • 著者名/発表者名: Zhao D, Ishikawa T, Quan L, Li DR, Michiue T, Maeda H.
  • 雑誌名: Legal Med. 10 ページ: 310-315
  • 査読あり
• [雑誌論文] 片側性血胸に合併した対側性外傷性気胸による急死の1剖検例. (2008)
  • 著者名/発表者名: 権力, 道上知美, 趙東, 李冬日, 石川隆紀, 前田均.
  • 雑誌名: 法医病理 14 ページ: 22-28
  • 査読あり
• [雑誌論文] Tissue-specific differences in mRNA quantification of glucose transporter 1 and vascular endothelial growth factor with special regard to death investigations of fatal injuries (2008)
  • 著者名/発表者名: Dong Zhao, Takaki Ishikawa, Li Quan, Dong-Ri Li, Tomoni Michiue, Chiemi Yoshida, Ayumi Komatsu, Jian-Hua Chen, Bao-Li Zhu, Hitoshi Maeda
  • 雑誌名: Forensic Science international 177 ページ: 176-183
• [雑誌論文] Single-stranded DNA as an immunohistochemical marker of neuronal damage in human brain:An analysis of autopsy material with reard to the cause of death (2008)
  • 著者名/発表者名: Tomomi Michiue, Takaki Ishikawa, Li Quan, Dong-Ri Li, Dong Zhao, Ayumi Komatsu, Bao-Li Zhu, Hitoshi Maeda
  • 雑誌名: Forensic Science international (Accepted)
• [学会発表] Postmortem catecholamine levels in pericardial and cerebrospinal fluids with regard to the cause of death in medicolegal autopsy (2008)
  • 著者名/発表者名: Quan L, Zhu B-L, Ishikawa T, Michiue T, Zhao D, Yoshida C, Chen J-H, Wang Q, Maeda H.
  • 学会等名: 7th International Symposium Advances in Legal Medicine, Sep, Jpn J Legal Med 2008
  • 発表場所: Osaka(62 :138)
• [学会発表] Postmortem serum erythropoietin and C-reactive Protein levels as markers of survival time in injury deaths (2008)
  • 著者名/発表者名: Quan L, Zhu B-L, Ishikawa T, Michiue T, Zhao D, Yoshida C, Chen J-H, Wang Q, Komatsu A, Azuma Y, Maeda H.
  • 学会等名: 7th International Symposium Advances in Legal Medicine, Sep, Jpn J Legal Med 2008
  • 発表場所: Osaka(62 : 138-139)
• [学会発表] Postmortem catecholamine levels in pericardial and cerebrospinal fluids with regard to the cause of death in medicolegal autopsy. (2008)
  • 著者名/発表者名: Quan L, Zhu B-L, Ishikawa T, Michiue T, Zhao D, Yoshida C, Chen J-H, Wang Q, Maeda H.
  • 学会等名: 7th International Symposium Advances in Legal Medicine
  • 発表場所: Osaka, Japan
• [学会発表] Postmortem serum erythropoietin and C-reactive Protein levels as markers of survival time in injury deaths. (2008)
  • 著者名/発表者名: Quan L, Zhu B-L, Ishikawa T, Michiue T, Zhao D, Yoshida C, Chen J-H, Wang Q, Komatsu A, Azuma Y, Maeda H.
  • 学会等名: 7th International Symposium Advances in Legal Medicine
  • 発表場所: Osaka, Japan
• [学会発表] lmmunohistochemistry of von Willebrand factor in the lungs with regard to the cause of death in forensic autopsy. (2008)
  • 著者名/発表者名: Quan L, Ishikawa T, Zhao D, Michiue T, Yoshida C, Chen J-H, Wang Q, Zhu B-L, Maeda H.
  • 学会等名: 7th International Symposium Advances in Legal Medicine
  • 発表場所: Osaka, Japan
• [学会発表] Analyses of cardiac blood cells and serum proteins with regard to cause of death in forensic autopsy cases. (2008)
  • 著者名/発表者名: Quan L, Ishikawa T, Michiue T, Zhao D, Yoshida C, Chen J-H, Komatsu A, Azuma Y, Zhu B-L, Maeda H.
  • 学会等名: 7th International Symposium Advances in Legal Medicine
  • 発表場所: Osaka, Japan
• [学会発表] Postmortem serum erythropoietin level with regard to cause of death and survival time in medicolegal autopsy. (2008)
  • 著者名/発表者名: Ouan L, Zhu B-L, Ishikawa T, Michiue T, Zhao D, Ogawa M, Maeda H.
  • 学会等名: 7th International Symposium Advances in Legal Medicine
  • 発表場所: Osaka, Japan
• [学会発表] Postmortem serum levels of pulmonary surfactant associated proteins A and D with regard to the cause of death in medicolegal autopsy. (2008)
  • 著者名/発表者名: Quan L, Zhu B-L, Ishikawa T, Michiue T, Zhao D, Yoshida C, Maeda H.
  • 学会等名: 7th International Symposium Advances in Legal Medicine
  • 発表場所: Osaka, Japan
• [学会発表] Postmortem serotonin levels in cerebrospinal and pericardial fluid with regard to the cause of death in medicolegal autopsy. (2008)
  • 著者名/発表者名: Quan L, Zhu B-L, Ishikawa T, Michiue T, Zhao D, Yoshida C, Chen J-H, Wang Q, Komatsu A, Azuma Y, Maeda H.
  • 学会等名: 7th International Symposium Advances in Legal Medicine
  • 発表場所: Osaka, Japan
• [学会発表] Postmortem lung weight with regard to survival time. (2008)
  • 著者名/発表者名: Chen J-H, Quan L, Ishikawa T, Michiue T, Wang Q, Zhu B-L, Maeda H.
  • 学会等名: 7th International Symposium Advances in Legal Medicine
  • 発表場所: Osaka, Japan
• [学会発表] Evaluation of postmortem calcium and magnesium levels in the pericardial fluid with regard to the cause of death in medicoleg al autopsy. (2008)
  • 著者名/発表者名: Li D-R, Quan L, Zhu B-L, I shikawa T, Michiue T, Zhao D, Yoshida C, Komatsu A, Azuma Y, Maeda H.
  • 学会等名: 7th International Symposium Advances in Legal Medicine
  • 発表場所: Osaka, Japan
• [学会発表] Quantitative analysis of single stranded DNA immunoreactivity as a marker of neuronal apoptosis in hippocampus with regard to the causes of death in medicolegal autopsy. (2008)
  • 著者名/発表者名: Michiue T, Quan L, Ishikawa T, Zhu B-L, Maeda H.
  • 学会等名: 7th International Symposium Advances in Legal Medicine
  • 発表場所: Osaka, Japan
• [学会発表] Evaluation of pulmonary GLUT1 and VEGF mRNA levels in relation to the lung weight in medicolegal autopsy cases. (2008)
  • 著者名/発表者名: Zhao D, Ishikawa T, Quan L, Michiue T, Yoshida C, Komatsu A, Chen J-H, Wang Q, Zhu B-L, Maeda H.
  • 学会等名: 7th International Symposium Advances in Legal Medicine
  • 発表場所: Osaka, Japan
• [学会発表] Sudden death following a struggle during arrest by the police : four autopsy cases. (2008)
  • 著者名/発表者名: Ishikawa T, Michiue T, Quan L, Zhu B-L, Maeda H.
  • 学会等名: International Association of Forensic Sciences 18th Triennial Meeting
  • 発表場所: New Orleans, USA.
• [学会発表] 剖検例における血清エジスロポェチン濃度の分析. (2008)
  • 著者名/発表者名: 権力, 朱宝利, 石川隆紀, 道上知美, 李冬日, 趙東, 吉田智美, 東洋子, 別所康守, 前田均
  • 学会等名: 第92次日本法医学会総会
  • 発表場所: 長崎
• [学会発表] 薬毒物中毒死剖検例の血液・体液内カテコラミンおよびセロトニン濃度. (2008)
  • 著者名/発表者名: 権力, 石川隆紀, 道上知美, 織谷茂樹, 小川雅史, 前田均.
  • 学会等名: 第30回日本中毒学会総会・学術集会, 中毒研究2008 ; 21 : 439-440
  • 発表場所: 和歌山
• [学会発表] 剖検例における脳神経細胞のアポトーシスに関する1本鎖DNAを指標とした定量形態学的分析 (2007)
  • 著者名/発表者名: 道上 知美, 石川 隆紀, 権 力, 李 冬日, 趙 東, 小松 亜由美, 朱 宝利, 前田 均
  • 学会等名: 第91次日本法医学会総会
  • 発表場所: 秋田県秋田ビューホテル
• [学会発表] Postmortem cardiac blood hematology for investigation of acute cause of death in medicolegal autopsy (2007)
  • 著者名/発表者名: Quan L, Ishikawa T, Li D-R, Zhao D, Michiue T, Chen J-H, Zhu B-L, Maeda H.
  • 学会等名: 9^<th> Indo-Pacific Congress on Legal Medicine & Forensic Sciences of The Indo-Pacific Association of Law, Medicine and Science
  • 発表場所: Sri Lanka
• [学会発表] Evaluation of postmortem serum and cerebrospiral fluid ACTH levels in relation to the cause of death in forensic autopsy (2007)
  • 著者名/発表者名: Ishikawa T, Quan L, Li D-R, Zhao D, Michiue T, Hamel M, Maeda H.
  • 学会等名: 9^<th> Indo-Pacific Congress on Legal Medicine & Forensic Sciences of The Indo-Pacific Association of Law, Medicine and Science
  • 発表場所: Sri Lanka
• [学会発表] Neuronal ischemic change and apoptosis in the hippocampus after brain injury (2007)
  • 著者名/発表者名: Li D-R, Ishikawa T, Quan L, Zhao D, Michiue T, Zhu B-L, Maeda H.
  • 学会等名: 9^<th> Indo-Pacific Congress on Legal Medicine & Forensic Sciences of The Indo-Pacifc Association of Law, Medicine and Science
  • 発表場所: Sri Lanka
• [学会発表] Tissue-specific patterns of glucose transporter 1 and vascular endoth elial growth factor mRNA expression in autopsy material with special regard to injury death (2007)
  • 著者名/発表者名: Zhao D, Ishikawa T, Quan L, Li D-R, Michiue T, Zhu B-L, Maeda H.
  • 学会等名: 9^<th> Indo-Pacific Congress on Legal Medicine & Forensic Sciences of The Indo-Pacific Association of Law, Medicine and Science
  • 発表場所: Sri Lanka
• [学会発表] Immunohistochemical distribution of single stranded DNA in the brain in medico-legal autopsy cases of carbon monoxide intoxication (2007)
  • 著者名/発表者名: Michiue T, Ishikawa T, Quan L, Li D-R, Zhao D, Komatsu A, Maeda H.
  • 学会等名: 9^<th> Indo-Pacific Congress on Legal Medicine & Forensic Sciences of The Indo-Pacific Association of Law, Medicine and Science
  • 発表場所: Sri Lanka