| 研究課題/領域番号 |
19F19061
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| 研究種目 |
特別研究員奨励費
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| 配分区分 | 補助金 |
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| 応募区分 | 外国 |
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| 審査区分 |
小区分27040:バイオ機能応用およびバイオプロセス工学関連
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| 研究機関 | 東京大学 |
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研究代表者 |
伊藤 大知 東京大学, 大学院医学系研究科(医学部), 教授 (50447421)
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| 研究分担者 |
CHANDEL ARVIND 東京大学, 医学(系)研究科(研究院), 外国人特別研究員
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| 研究期間 (年度) |
2019-04-25 – 2021-03-31
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| 研究課題ステータス |
完了 (2020年度)
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| 配分額 *注記 |
2,300千円 (直接経費: 2,300千円)
2020年度: 1,100千円 (直接経費: 1,100千円)
2019年度: 1,200千円 (直接経費: 1,200千円)
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| キーワード | hydrogels / antiadhesion barrier / dextran / PEG / hyaluronan / Anti-adhesion / Hemostatic / Injectable / Hydrogel / Biocompatible |
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| 研究開始時の研究の概要 |
The purpose of this study is to develop an antiadhesive, hemostatic, and antimicrobial smart polymeric material which overcomes the limits of available antiadhesive and haemostatic materials. Injectable hydrogel will be generated via block copolymer self-assembly, using copolymers of dextran and PDMS containing aldehyde and tertiary amine or acrylate. End-functionalized polyphosphate will be developed as in situ crosslinking agents. Gelation kinetics, biocompatibility, drug encapsulation/release property, and hemostatic/anti-adhesion property of the obtained material will be examined.
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| 研究実績の概要 |
We have developed an injectable hydrogel via Dex-g-PDPMA-g-SRT crosslinked with Cl-PEG-Cl. Partially oxidized Dextran was grafted with Poly[2 (Dimethylamino)ethyl Methacrylate] (PDMAEMA) by ATRP to form Dex-g-PDMAEMA. Serotonin (SRT) was reacted with partially oxidized aldehyde containing Dex-g-PDMAEMA to form SRT-g-Dex-PDMAEMA, and chloride terminated poly(ethylene glycol) (Cl-PEG-Cl)was prepared 4-chloromethyl benzoyl chloride by the esterification reaction. The chemically crosslinked hydrogel was obtained, by the reaction between tertiary amine group of PDMAEMA and chloride terminated PEG. The gelation time of the hydrogel was 2-5 min its basically depends on concentration of the prepolymers solution and temperature. The In-vivo hemostatic behavior of hydrogel was evaluated using mice liver hemorrhage model. The hydrogel showed excellent hemostatic capacity in comparison to control. The hemostatic behavior of the hydrogel due to two component first formation of permanent positive charge on tertiary amine of PDMAEMA upon crosslinking and second one is pendent serotonin moiety. The hydrogel was compared with marketed hemostatic product TachoSil; and SURGICEL; using mice liver hemorrhage model. The hydrogel follows dual hemostatic mechanism, because it contains permanent cationic change that interact with red blood cells and serotonin moiety interact with platelet and accelerate the hemostasis synergistically.
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| 現在までの達成度 (段落) |
令和2年度が最終年度であるため、記入しない。
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| 今後の研究の推進方策 |
令和2年度が最終年度であるため、記入しない。
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