| 研究課題/領域番号 |
19F19718
|
|---|
| 研究種目 |
特別研究員奨励費
|
| 配分区分 | 補助金 |
|---|
| 応募区分 | 外国 |
|---|
| 審査区分 |
小区分46010:神経科学一般関連
|
|---|
| 研究機関 | 国立研究開発法人理化学研究所 |
|---|
研究代表者 |
風間 北斗 国立研究開発法人理化学研究所, 脳神経科学研究センター, チームリーダー (90546574)
|
|---|
| 研究分担者 |
KIRSZENBLAT LEONIE 国立研究開発法人理化学研究所, 脳神経科学研究センター, 外国人特別研究員
|
|---|
| 研究期間 (年度) |
2019-07-24 – 2021-03-31
|
| 研究課題ステータス |
完了 (2020年度)
|
| 配分額 *注記 |
2,300千円 (直接経費: 2,300千円)
2020年度: 1,100千円 (直接経費: 1,100千円)
2019年度: 1,200千円 (直接経費: 1,200千円)
|
|---|
| キーワード | sleep / olfaction / calcium imaging / optogenetics / Drosophila |
|---|
| 研究開始時の研究の概要 |
Sleep is a state of quiescence during which an animal loses awareness of the external world. In this study, we will investigate the neural mechanisms by which the brain suppresses perception during sleep, with a focus on the Drosophila olfactory system.
|
| 研究実績の概要 |
We have made efforts toward building a set up in which olfactory circuits can be imaged under the microscope while simultaneously monitoring the fly’s behavior and detecting when it is asleep. We have also engineered a fly strain in which we can simultaneously induce sleep and monitor neural activity. The behavior of these flies was examined and it was found that upon exposure to 617nm red-light, the flies become quiescent, and when the light is switched off they become active again. These experiments confirm that this optogenetic tool can be used as a ‘sleep switch’ to transiently induce sleep by activating specific neurons in the fly brain. We then examined the activity of these neurons using calcium imaging, and confirmed that neural activity is increased during red light exposure. In parallel to testing sleep, we have also begun to record brain responses to different odors, allowing us to optimize our imaging settings for future sleep and sleep deprivation experiments.
We expect our study will provide fundamental insights into how sleep affects sensory processing. Since a number of studies have shown that sleep in the fruit fly, Drosophila, shares many similarities with sleep in humans and other animals, any of the neural mechanisms and functions of sleep that we uncover here will likely be conserved in other animals.
|
| 現在までの達成度 (段落) |
令和2年度が最終年度であるため、記入しない。
|
| 今後の研究の推進方策 |
令和2年度が最終年度であるため、記入しない。
|
