| 研究課題/領域番号 |
19F19764
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| 研究種目 |
特別研究員奨励費
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| 配分区分 | 補助金 |
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| 応募区分 | 外国 |
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| 審査区分 |
小区分43020:構造生物化学関連
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| 研究機関 | 沖縄科学技術大学院大学 |
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研究代表者 |
LAURINO Paola 沖縄科学技術大学院大学, タンパク質工学・進化ユニット, 准教授 (90812256)
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| 研究分担者 |
DINDO MIRCO 沖縄科学技術大学院大学, タンパク質工学・進化ユニット, 外国人特別研究員
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| 研究期間 (年度) |
2019-11-08 – 2022-03-31
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| 研究課題ステータス |
完了 (2021年度)
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| 配分額 *注記 |
2,100千円 (直接経費: 2,100千円)
2021年度: 900千円 (直接経費: 900千円)
2020年度: 1,100千円 (直接経費: 1,100千円)
2019年度: 100千円 (直接経費: 100千円)
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| キーワード | Phase separation / enzymes / enzyme kinetic / protein engineering / protein plasticity / Disordered regions / AGT / protocells / Liquid phase separation / Biochemistry / enzyme / library / screening |
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| 研究開始時の研究の概要 |
The DPAT enzyme will be fully characterised from structural and functional perspectives, in order to understand its unusual activity in detail. Laurino group already has an established collaboration with the Jackson Lab at ANU, Australia and they will help to solve the structure of this enzyme. Then, BLAST searches will help us to find proteins structurally related to DPAT, in order to identify possible homologs with the same or similar functions and to expand our knowledge of this interesting class of enzymes. Finally the recombinant DPAT will be engineered by rounds of directed evolution.
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| 研究実績の概要 |
・The production/generation of liquid-liquid phase separated membrane-less droplets with novel features as the ability to compartmentalize enzymes efficiently, high protein crowding (mM protein content) to study in detail the effects of crowding on proteins and especially on the enzymatic activity (using length scale of living systems micrometer scale as cells).
・We also demonstrated how the enzymatic activity in presence of crowding generated flow similar to the cytoplasmatic streaming of the cell. This work has been published by Nature Communications it is amongst the 2021 top 25 most downloaded Chemistry and Material Science Articles in 2021 published in Nature communication.
・In addition, we have discovered how protein plasticity shape the function and fitness. The work has been published in Protein Science.
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| 現在までの達成度 (段落) |
令和3年度が最終年度であるため、記入しない。
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| 今後の研究の推進方策 |
令和3年度が最終年度であるため、記入しない。
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