| 研究課題/領域番号 |
19F19782
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| 研究種目 |
特別研究員奨励費
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| 配分区分 | 補助金 |
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| 応募区分 | 外国 |
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| 審査区分 |
小区分44030:植物分子および生理科学関連
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| 研究機関 | 国立研究開発法人理化学研究所 |
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研究代表者 |
白須 賢 国立研究開発法人理化学研究所, 環境資源科学研究センター, グループディレクター (20425630)
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| 研究分担者 |
FISHMAN MAX 国立研究開発法人理化学研究所, 環境資源科学研究センター, 外国人特別研究員
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| 研究期間 (年度) |
2019-11-08 – 2022-03-31
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| 研究課題ステータス |
完了 (2021年度)
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| 配分額 *注記 |
2,200千円 (直接経費: 2,200千円)
2021年度: 700千円 (直接経費: 700千円)
2020年度: 1,100千円 (直接経費: 1,100千円)
2019年度: 400千円 (直接経費: 400千円)
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| キーワード | Parasiticplants / Calciumsignaling / phosphoproteomics / DMBQ / hustorium / parasiticplants / calciumsignaling / haustorium / parasitic plants / calcium signaling |
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| 研究開始時の研究の概要 |
The molecular biology and signaling pathway that initiates haustorium induction in parasitic flowering plants is not well characterized. Haustorium induction can be initiated in Phtheirospermum japonicum with the haustorium inducing factor dimethoxy benzoquinone (DMBQ). I plan to use P. japonicum in order to identify primary signaling components responsible for haustorium induction following DMBQ treatment. My goal is to identify receptor-like kinases (RLKs), co-receptors, or transcription factors found within the primary signaling pathway responsible for DMBQ-induced haustorium development.
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| 研究実績の概要 |
During 2021, I identified endogenous Phtheirospermum japonicum peptide hormones that are induced upon treatment with the haustorium inducing compound (HIF) 2,6 dimethoxybenzoquinone (DMBQ). I found that treatment of P. japonicum with an endogenous peptide induced haustorium formation without the treatment of an HIFs and induced expression of the known haustorium formation marker gene PjYUC3. Further investigation revealed that there are three additional putative peptide hormones in the P. japonicum genome and six putative peptide receptors. Further investigation found that only one of those three peptides is up-regulated following DMBQ treatment while none of the receptors are up-regulated following DMBQ treatment. I further characterized the expression of the peptides and receptors using transformed P. japonicum hairy roots expressing reporter gene constructs. I found that only one of the four peptides and potentially three of the receptors are expressed in the haustorium following DMBQ treatment. Lastly, PLETHORA (PLT) transcription factors are stabilized post-translationally by the peptides. Using hairy root transformation, I found that DMBQ treatment resulted in movement of a PjPLT protein up the root and that this PjPLT localized in the haustorium.
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| 現在までの達成度 (段落) |
令和3年度が最終年度であるため、記入しない。
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| 今後の研究の推進方策 |
令和3年度が最終年度であるため、記入しない。
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