| 研究課題/領域番号 |
19F19805
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| 研究種目 |
特別研究員奨励費
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| 配分区分 | 補助金 |
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| 応募区分 | 外国 |
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| 審査区分 |
小区分32010:基礎物理化学関連
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| 研究機関 | 東京大学 |
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研究代表者 |
合田 圭介 東京大学, 大学院理学系研究科(理学部), 教授 (70518696)
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| 研究分担者 |
GALA DE PABLO JULIA 東京大学, 理学(系)研究科(研究院), 外国人特別研究員
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| 研究期間 (年度) |
2019-11-08 – 2022-03-31
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| 研究課題ステータス |
完了 (2021年度)
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| 配分額 *注記 |
2,200千円 (直接経費: 2,200千円)
2021年度: 500千円 (直接経費: 500千円)
2020年度: 600千円 (直接経費: 600千円)
2019年度: 1,100千円 (直接経費: 1,100千円)
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| キーワード | FT-CARS / Raman / Microalgae / Carbohydrate / Paramylon / Euglena / Starch / Carotenoid / Raman Microalgae / Euglena Starch / Carbohydrate Paramylon |
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| 研究開始時の研究の概要 |
1) System training with standard samples and human leukaemia cell line cells.
2) Optimization of the microfluidic design and fabrication.
3) Multivariate analysis optimization and classification models development, integrating bright field imaging information.
4) Tests on healthy human blood and on leukaemia samples. Analysis of SS patient samples cell heterogeneity. In-vitro drug treatment response analysis.
5) Tests with CTC samples.
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| 研究実績の概要 |
The system pulse-shaping capabilities were improved and a transform-limited pulse was achieved, improving the sensitivity and signal quality for carbohydrates and carotenoids. Heavy ions mutagenesis was performed in Euglena gracilis, identifying hyperaccumulating mutants via FT-CARS paramylon. RNA isolation was done and cDNA analysis was performed. Analysis of this data and manuscript preparation is currently in process. The analysis of starch accumulation in Chromochloris zofingiensis via both FT-CARS and enzymatic analysis were done, and a manuscript is in preparation. Sorting of multiple microalgae species was achieved and findings were recently published in Advanced Materials Technology. Human blood signal was obtained via FT-CARS, likely due to the strong resonant Raman of haemoglobin. However, leukocyte detection is still challenging with the current system sensitivity, and thus the system will require further optimization for clinical applications. An additional application of the FT-CARS system was identified: microplastics detection under flow. Preliminary work was done and has been followed by other researchers after the fellow’s departure.
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| 現在までの達成度 (段落) |
令和3年度が最終年度であるため、記入しない。
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| 今後の研究の推進方策 |
令和3年度が最終年度であるため、記入しない。
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