| 配分額 *注記 |
4,420千円 (直接経費: 3,400千円、間接経費: 1,020千円)
2021年度: 1,300千円 (直接経費: 1,000千円、間接経費: 300千円)
2020年度: 1,560千円 (直接経費: 1,200千円、間接経費: 360千円)
2019年度: 1,560千円 (直接経費: 1,200千円、間接経費: 360千円)
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| 研究開始時の研究の概要 |
Vertebrates consists of two major groups: jawed vertebrates (mammals, reptiles, fish, etc) and jawless vertebrates (lampreys and hagfishes). While much is known about the formation of blood cells in mammals and fish, how these are formed in more distant vertebrates - both jawless and jawed- is unkown.
My research line hence focuses on studying some of these group of vertebrates for which our knowledge about their hematopoiesis system is limited.
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| 研究実績の概要 |
Hematopoiesis is the process by which the blood cells of an individual, from the embryo to adulthood, are formed. However, despite being a crucial physiological phenomenon of
animals, most of our knowledge derives from studies using a handful of classic animal models, mostly representatives of jawed vertebrates. With
this in mind, I set to study the lamprey, long-distant relative of jawed vertebrates that is understudied in terms of the blood system. In FY2020 I completed the analysis of RNA-seq data from lamprey embryos and identified all candidate hematopoietic markers in the lamprey. I generated a complete panel of expression patterns for all putative hematopoietic markers during lamprey embryogenesis. I was then able to perform CRISPR knock-outs of lamprey Scl gene, an important hematopoietic factor in other vertebrates, and obtained enough samples to carry out RNA-seq analysis as well as in situ hybridization experiments. Although there was no apparent mutant phenotype, blood alterations are expected. These samples will be used in the near future to assess the function of Scl in blood development in cyclostomes, what will enable comnparative analysis with gnathostome hematopoiesis.
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