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Multi-omics authentication of global and adulterated Japanese non-centrifugal brown sugars

研究課題

研究課題/領域番号 19K15784
研究種目

若手研究

配分区分基金
審査区分 小区分38050:食品科学関連
研究機関琉球大学

研究代表者

ASIKIN YONATHAN  琉球大学, 農学部, 准教授 (20799689)

研究期間 (年度) 2019-04-01 – 2023-03-31
研究課題ステータス 交付 (2021年度)
配分額 *注記
4,290千円 (直接経費: 3,300千円、間接経費: 990千円)
2021年度: 1,430千円 (直接経費: 1,100千円、間接経費: 330千円)
2020年度: 1,430千円 (直接経費: 1,100千円、間接経費: 330千円)
2019年度: 1,430千円 (直接経費: 1,100千円、間接経費: 330千円)
キーワードFood flavor quality / Non-centrifugal sugar / Mineral composition / MS-e-nose profiling / Brown sugar / Food authentication / Multi-omics profiling
研究開始時の研究の概要

Non-centrifugal brown sugar is widely produced in many countries under different forms and flavor characteristics. Hence, the global trade era has urge domestic sugar industries to take measure on fraud and adulteration threats. This study aims to elucidate nutrient and flavor markers of brown sugars across the globe through multi-omics analytical technique, and thus to advance the building efforts of international product identity for brown sugar against adulteration. Moreover, authentication of brown sugar flavor can be unraveled through improved MS-based-e-nose omics profiling technology.

研究実績の概要

Volatile flavor and mineral compositions of non-centrifugal sugar (NCS) products derived from cane and palm of Japan and overseas origins were evaluated. A number of volatile components were identified in cane and palm NCSs (50 and 53 compounds; averaging 29.7 and 34.5 mg/100 g, respectively). Multivariate statistical model showed that cane NCS of domestic origins were mostly rich in Maillard reaction products such dimetylpyrazines. Moreover, five Okinawan specialty NCSs (kokuto) from Nakijin Village, Uruma City, Urasoe City, Ie Island, and Kohama Island were distinguished from other produces for containing combination of sweet-nutty and pungent aromas from trimethylpyrazine, 2-ethyl-6-methylpyrazine, 2,3-butanediol, and acetic acid. On the other hand, palm NCSs contained higher 3,5-dihydroxy-6-methyl-2,3-dihydro-4H-pyran-4-one (sweet-caramel odor). Additionally, cane NCSs were comprised of K, Ca, Mg, P, and Na (67.4, 16.4, 7.7, 5.1 and 2.6%, respectively), followed by small amounts of Fe, Zn, Mn, and Cu, wherein domestic cane NCSs contained higher minerals than that of overseas origins (1055.7 vs. 736.7 mg/100 g). Then, mineral content in Okinawan kokuto could be remarkably distinguished from its raw sugar blended-processed form (kako-kokuto) (1268.5 vs. 228.3 mg/100 g). Conversely, the average amount of minerals in palm NCSs was 1221.2 mg/100 g, comprising of K, Na, P, Mg, and Ca (72.1, 16.9, 5.2, 3.8, and 1.6%, respectively). These outcomes revealed that raw material and production origin greatly influenced flavor and nutrient profiles of NCSs, and thus their qualities.

現在までの達成度 (区分)
現在までの達成度 (区分)

4: 遅れている

理由

The study has focused on profiling of key flavor and nutrient properties of non-centrifugal sugar (NCS) of both domestic and overseas origins. However, the progress of authentication assessment of domestic NCS products is delayed because producing simulated adulterated NCS models, particularly on the optimization of pilot-scale production, takes longer time than expected.

今後の研究の推進方策

Profiling of bioactive phenolic compounds and their food functionalities will be further conducted for domestic and foreign non-centrifugal sugar (NCS) products that had been collected from the previous fiscal year. Moreover, the authentication assessment of flavor quality of domestic NCS will be performed against experimentally prepared models of adulterated NCS. Their food components will be evaluated using HPLC or NMR measurement systems, and volatile flavor compounds using SPME-GC-MS analysis.

報告書

(3件)
  • 2021 実施状況報告書
  • 2020 実施状況報告書
  • 2019 実施状況報告書

研究成果

(7件)

すべて 2021 2019 その他

すべて 国際共同研究 (1件) 雑誌論文 (1件) (うち国際共著 1件、 査読あり 1件) 学会発表 (5件) (うち国際学会 3件、 招待講演 2件)

  • [国際共同研究] Naresuan University(タイ)

    • 関連する報告書
      2019 実施状況報告書
  • [雑誌論文] Physicochemical, antioxidant, volatile component, and mass spectrometry-based electronic nose analyses differentiated unrefined non-centrifugal cane, palm, and coconut sugars2021

    • 著者名/発表者名
      Monthana Weerawatanakorn, Yonathan Asikin, Kanyaphat Meerod, Hajime Tamaki, Koji Wada
    • 雑誌名

      Journal of Food Measurement and Characterization

      巻: 15 (2) ページ: 1563-1577

    • DOI

      10.1007/s11694-020-00749-x

    • 関連する報告書
      2020 実施状況報告書
    • 査読あり / 国際共著
  • [学会発表] GC-MS based electronic nose profiling of regional brown sugars2021

    • 著者名/発表者名
      Yonathan Asikin
    • 学会等名
      The 6th International Conference on Food, Agriculture, and Natural Resources 2021
    • 関連する報告書
      2021 実施状況報告書
    • 国際学会 / 招待講演
  • [学会発表] Improvement of non-centrifugal brown sugar quality through food science study2021

    • 著者名/発表者名
      Yonathan Asikin
    • 学会等名
      The 1st International Conference on Applied Science and Technology for Community Service
    • 関連する報告書
      2021 実施状況報告書
    • 国際学会 / 招待講演
  • [学会発表] 黒糖製造工程の仕上げ加熱の違いによる物理化学的特性および香気成分の分析2021

    • 著者名/発表者名
      仲座佑紀, Yonathan Asikin, 大江萌那, 高良健作, 前田剛希, 土田永渡, 和田浩二
    • 学会等名
      日本食品保蔵科学会第70回大会
    • 関連する報告書
      2021 実施状況報告書
  • [学会発表] Sugar, organic acid, and MS-e-nose properties of non-centrifugal cane sugars from Japan and ASEAN countries2021

    • 著者名/発表者名
      Asikin Yonathan, Wada Koji, Tsuchida Eito, Maeda Goki, Nam Vu Tuan, Ayustaningwarno Fitriyono, Hajar-Azhari Siti
    • 学会等名
      The 2021 Annual Meeting of Japan Society for Bioscience, Biotechnology, and Agrochemistry 日本農芸化学会2021年度大会
    • 関連する報告書
      2020 実施状況報告書
  • [学会発表] Physico-chemical quality discrimination and volatile flavor profiling of regional non-centrifugal sugar made from sugarcane, palm, and coconut2019

    • 著者名/発表者名
      Yonathan Asikin, Miyako Kusano, Monthana Weerawatanakorn, Kanyaphat Meerod, Hajime Tamaki, Koji Wada
    • 学会等名
      13th Asian Congress of Nutrition, Bali, Indonesia, アジア栄養学会連合 (Federation of Asian Nutrition Societies: FANS) (Ann Nutr Metab 2019;75:1-424: DOI: 10.1159/000501751)
    • 関連する報告書
      2019 実施状況報告書
    • 国際学会

URL: 

公開日: 2019-04-18   更新日: 2022-12-28  

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