| 研究課題/領域番号 |
19K15846
|
|---|
| 研究種目 |
若手研究
|
| 配分区分 | 基金 |
|---|
| 審査区分 |
小区分39040:植物保護科学関連
|
|---|
| 研究機関 | 国立研究開発法人理化学研究所 |
|---|
研究代表者 |
GAN PAMELA 国立研究開発法人理化学研究所, 環境資源科学研究センター, 研究員 (80634740)
|
|---|
| 研究期間 (年度) |
2019-04-01 – 2021-03-31
|
| 研究課題ステータス |
中途終了 (2020年度)
|
| 配分額 *注記 |
4,290千円 (直接経費: 3,300千円、間接経費: 990千円)
2020年度: 1,300千円 (直接経費: 1,000千円、間接経費: 300千円)
2019年度: 2,990千円 (直接経費: 2,300千円、間接経費: 690千円)
|
|---|
| キーワード | Genome rearrangement / cluster / virulence / fungal genome / anthracnose / nudix / effector / Keywords / fungi / genome / evolution / Colletotrichum / pathogen / Pathogen / Evolution / Fungi / Effector |
|---|
| 研究開始時の研究の概要 |
Understanding how virulence evolves in pathogens can contribute to the development of better methods of disease control. We discovered a virulent strain-specific gene cluster in Colletotrichum fungi that has a variable distribution across multiple species. We will generate chromosome level assemblies for different species with this virulence cluster to gain insights into the evolution and transmission of these genes. We will also functionally characterize a gene within the cluster, which has been shown in preliminary experiments to suppress basal plant defense responses.
|
| 研究実績の概要 |
Understanding how virulence emerges in pathogens can contribute to the development of better methods of disease control. We discovered a virulent strain-specific cluster of genes in Colletotrichum fungi which cause strawberry anthracnose that has an uneven phylogenetic distribution across multiple species. In FY2020, the following was achieved:
1. Submitted paper describing potential virulence gene clusters in sub-telomeric regions and repeat-rich chromosomes. The work and associated genome and transcriptome data has been publicly released on NCBI, github and Biorxiv and is currently under review in the journal Genome Research.
2. One of the proteins present in the potential virulence gene cluster, CfNudix, was characterized further. Phylogenetic analyses of all C. fructicola nudixes including CfNudix was carried out to determine the most closely related sequences to identify potential substrates by homology to previously characterized nudixes. The substrate specificity of purified CfNudix was tested on known nudix substrates.
|
