| 研究課題/領域番号 |
19K16517
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| 研究種目 |
若手研究
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| 配分区分 | 基金 |
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| 審査区分 |
小区分48040:医化学関連
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| 研究機関 | 名古屋大学 |
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研究代表者 |
LO PEIWEN 名古屋大学, 医学系研究科, 研究機関研究員 (20822406)
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| 研究期間 (年度) |
2019-04-01 – 2021-03-31
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| 研究課題ステータス |
中途終了 (2020年度)
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| 配分額 *注記 |
4,290千円 (直接経費: 3,300千円、間接経費: 990千円)
2022年度: 780千円 (直接経費: 600千円、間接経費: 180千円)
2021年度: 1,430千円 (直接経費: 1,100千円、間接経費: 330千円)
2020年度: 1,300千円 (直接経費: 1,000千円、間接経費: 300千円)
2019年度: 780千円 (直接経費: 600千円、間接経費: 180千円)
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| キーワード | O-GlcNAcylation / Notch1 / aging / muscle / satellite cell / regeneration / skeletal muscle |
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| 研究開始時の研究の概要 |
Muscle regeneration declines with aging and interferes life of numerous aged people. Nevertheless, the therapy for ameliorating degeneracy of muscle regeneration is limited thus far. It has been known that Notch1 contributes to decline of muscle regeneration with aging and O-GlcNAcylation, implicates in Notch1 signaling pathway. Thus, we speculate that O-GlcNAcylation may be involved with muscle regeneration via Notch1 protein. We expect this study could be conductive to the improvement of muscle rejuvenation for aged people and the therapy for muscular dystrophy patients.
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| 研究実績の概要 |
We speculated declined muscle self-renewal with aging is mediated partially by the O-GlcNAcylation on Notch1.
To understand if the O-GlcNAcylation status of Notch1 is altered with aging, I established the inducible FLAG-Notch1 transgenic mice. Afterwards, O-GlcNAcylation on Notch1 protein extracted from aged and younger mice will be applied in glycosylation analysis.
Albeit Eogt didn’t be detected in satellite cells of muscle, I found Eogt distributes only in “certain” myofibers. In further experiments, I found Eogt highly overlaps myofiber type2A.
The mouse hanging assay also shows Eogt knockdown reduces muscle contractibility.
It suggests that Eogt is involved in muscle contractibility by mediating myofiber types. This study facilitates to ameliorate mobility of aged people.
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