| 研究課題/領域番号 |
19K19058
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| 研究種目 |
若手研究
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| 配分区分 | 基金 |
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| 審査区分 |
小区分57040:口腔再生医学および歯科医用工学関連
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| 研究機関 | 東京歯科大学 |
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研究代表者 |
楊 隆強 東京歯科大学, 歯学部, 助教 (30822473)
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| 研究期間 (年度) |
2019-04-01 – 2022-03-31
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| 研究課題ステータス |
中途終了 (2021年度)
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| 配分額 *注記 |
3,770千円 (直接経費: 2,900千円、間接経費: 870千円)
2021年度: 1,170千円 (直接経費: 900千円、間接経費: 270千円)
2020年度: 1,300千円 (直接経費: 1,000千円、間接経費: 300千円)
2019年度: 1,300千円 (直接経費: 1,000千円、間接経費: 300千円)
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| キーワード | dental pulp regeneration / tissue engineering / collagen biomaterials / dental pulp stem cells / gelatin / β-TCP / magesium / magnesium / treated dentin matrix / collagen biomaterial / collagen sponge / dental stem cells |
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| 研究開始時の研究の概要 |
Dental pulp diseases have caused great burdens on the public, compromised patients' quality of life and cost huge amount of money. Although the removal of infected or necrotic dental pulp by root canal treatment relieves pain and preserves teeth, devitalized teeth may become more brittle and prone to structural failure over time. Functional dental pulp regeneration has gained enormous attention as an alternative to root canal treatment. We aim to develop an innovative method to achieve fully functional dental pulp regeneration with new blood vessels and sensory nerves for clinical treatments.
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| 研究実績の概要 |
Dental pulp diseases severely compromise patients' quality of life and cause huge economic burden. Functional dental pulp regeneration has gained enormous attention as an alternative to root canal treatment. This study aimed to develop an innovative method to achieve functional dental pulp regeneration with new blood vessels and sensory nerves for future clinical treatments. Generally, key elements including suitable biomaterial scaffolds, bioactive molecules and dental pulp stem cells are needed for tissue regeneration. The successful fabrication of atelocollagen/gelatin (ACG) sponge holds great potential as it shares the main component of collagen with dental pulp tissue. ACG sponge could also be modified or incorporated with bioactive reagents as a drug delivery system. Heat treatment in the vacuum, which did not introduce any toxic crosslinking reagent, effectively improved the structural stability of ACG sponge. Moreover, ACG/β-TCP/Mg2+ composite sponge had been successfully fabricated and showed similar porous microstructures. Bioactive β-TCP and Mg2+ were shown to be fully incorporated into ACG sponge as revealed by SEM and XPS analyses. Dental pulp stem cells were isolated and cultured from incisors of 4-week-old SD rats. The optimal concentration of Mg2+ for dental pulp stem cells proliferation and differentiation was confirmed in vitro. ACG/β-TCP/Mg2+ composite sponge did not compromise cell growth and proliferation on the sponge surface. These indicate that ACG sponge combined with bioactive molecules may pose the possibility for tissue engineering.
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