核酸機能の効果的時空間解析のための励起子制御イメージング

• 研究課題/領域番号: 20350083
• 研究種目: 基盤研究(B)
• 配分区分: 補助金
• 応募区分: 一般
• 研究分野: 生体関連化学
• 研究機関: 独立行政法人理化学研究所
• 研究代表者: 岡本 晃充 独立行政法人理化学研究所, 岡本独立主幹研究ユニット, ユニットリーダー(独立主幹研究員) (60314233)
• 研究期間 (年度): 2008 – 2010
• 研究課題ステータス: 完了 (2010年度)
• 配分額: 19,240千円 (直接経費: 14,800千円、間接経費: 4,440千円); 2010年度: 6,240千円 (直接経費: 4,800千円、間接経費: 1,440千円); 2009年度: 6,890千円 (直接経費: 5,300千円、間接経費: 1,590千円); 2008年度: 6,110千円 (直接経費: 4,700千円、間接経費: 1,410千円)
• キーワード: 励起子相互作用 / 蛍光イメージング / 蛍光色素 / DNA / 核酸 / 蛍光 / 色素 / イメージング

研究概要

励起子相互作用による蛍光消光を効果的に使用した新しい発想のオン-オフ蛍光核酸プローブ(近赤外)を設計・作成した。このプローブは、プローブだけのときには色素の二量化によってほとんど蛍光を示さないが、標的配列(DNA・RNA)とハイブリダイゼーションしたとき二量体構造が崩れ、強い蛍光発光を与えた。

研究成果

• [雑誌論文] ECHO-LNA Conjugates : Hybridization-sensitive Fluorescence and its Application to Fluorescent Detection of Various RNA Strands (2010)
  • 著者名/発表者名: Sugizaki K.; Okamoto A.
  • 雑誌名: Bioconjugate Chem. 21(12) ページ: 2276-2281
  • 査読あり
• [雑誌論文] Sets of RNA Repeated Tags and Hybridization-Sensitive Fluorescent Probes for Distinct Images of RNA in a Living Cell (2010)
  • 著者名/発表者名: Kubota T.; Ikeda S.; Yanagisawa H.Yuki M.; Okamoto A
  • 雑誌名: PLoS ONE 5(9)
  • 査読あり
• [雑誌論文] Excitonic interaction : Another photophysical process for fluorescence-controlled nucleic acid sensing (2010)
  • 著者名/発表者名: Okamoto A
  • 雑誌名: Chem.Rec. 10(3) ページ: 188-196
  • 査読あり
• [雑誌論文] A Probe Containing Two Base-discriminating Fluorescent (BDF) Nucleosides for SNP Typing (2010)
  • 著者名/発表者名: Yoshida Y.; Niwa K.; Yamada K.; Tokeshi M.; Baba Y.; Saito Y.Okamoto A.; Saito I
  • 雑誌名: Chem. Lett. 39(2) ページ: 116-117
  • NAID: 10027177147
  • 査読あり
• [雑誌論文] Exciton-Primer Mediated SNP Detection in SmartAmp2 Reactions (2010)
  • 著者名/発表者名: Lezhava A.; Ishidao T.; Ishizu Y.; Naito K.; Hanami T.; Katayama A.; Kogo Y.; Soma T.; Ikeda S.; Murakami K.; Nogawa C.; Itch M.; Mitani Y.; Harbers M.; Okamoto A.; Hayashizaki Y
  • 雑誌名: Hum.Mutat. 31(2) ページ: 208-217
  • 査読あり
• [雑誌論文] Hybridization-sensitive fluorescent DNA probe with self-avoidance ability (2010)
  • 著者名/発表者名: Ikeda S.; Kubota T.; Yuki M.; Yanagisawa H.; Tsuruma S.; Okamoto A
  • 雑誌名: Org.Biomol.Chem. 8(3) ページ: 546-551
  • 査読あり
• [雑誌論文] Hybridization-sensitive fluorescent DNA probe with self-avoidance ability (2010)
  • 著者名/発表者名: Ikeda, S.
  • 雑誌名: Org.Biomol.Chem. 巻: 8(3) ページ: 546-551
  • 査読あり
• [雑誌論文] Exciton-Primer Mediated SNP Detection in SmartAmp2 Reactions (2010)
  • 著者名/発表者名: Lezhava, A.
  • 雑誌名: Hum.Mutat. 巻: 31(2) ページ: 208-217
  • 査読あり
• [雑誌論文] A Probe Containing Two Base-discriminating Fluorescent (BDF) Nucleosides for SNP Typing (2010)
  • 著者名/発表者名: Yoshida, Y.
  • 雑誌名: Chem.Lett. 巻: 39(2) ページ: 116-117
  • 査読あり
• [雑誌論文] Excitonic interaction : Another photophysical process for fluorescence-controlled nucleic acid sensing (2010)
  • 著者名/発表者名: Okamoto, A.
  • 雑誌名: Chem.Rec. 巻: 10(3) ページ: 188-196
  • 査読あり
• [雑誌論文] Sets of RNA Repeated Tags and Hybridization-Sensitive Fluorescent Probes for Distinct Images of RNA in a Living Cell (2010)
  • 著者名/発表者名: Kubota, T.
  • 雑誌名: PLoS ONE 巻: 5(9)
  • 査読あり
• [雑誌論文] Hybridization-sensitive Fluorescence and its Application to Fluorescent Detection of Various RNA Strands (2010)
  • 著者名/発表者名: Sugizaki, K.
  • 雑誌名: Bioconjugate Chem. 巻: 21(12) ページ: 2276-2281
  • 査読あり
• [雑誌論文] Hybridization-sensitive fluorescent DNA probe with self-avoidance ability (2010)
  • 著者名/発表者名: Ikeda, S.
  • 雑誌名: Org.Biomol.Chem. 8(3) ページ: 546-551
  • 査読あり
• [雑誌論文] Doubly thiazole orange-labeled cytidine for functional expansion of a hybridization-sensitive probe (2009)
  • 著者名/発表者名: Ikeda S.; Yuki M.; Yanagisawa H.; Okamoto A
  • 雑誌名: Tetrahedron Lett. 50(51) ページ: 7191-7195
  • 査読あり
• [雑誌論文] Exciton-Controlled Hybridization- Sensitive Fluorescent Probes : Multicolor Detection of Nucleic Acids (2009)
  • 著者名/発表者名: Ikeda S.; Kubota T.; Yuki M.; Okamoto A
  • 雑誌名: Angew.Chem., Int.Ed. 48(35) ページ: 6480-6484
  • 査読あり
• [雑誌論文] Hybridization-Sensitive Fluorescent Probe for Long-Time Monitoring of Intracellular RNA (2009)
  • 著者名/発表者名: Kubota T.; Ikeda S.; Yanagisawa H.; Yuki M.; Okamoto A
  • 雑誌名: Bioconjuga to Chem. 20(6) ページ: 1256-1261
  • 査読あり
• [雑誌論文] Photoresponsive Tandem Zinc Finger Peptide (2009)
  • 著者名/発表者名: Nomura A.; Okamoto A
  • 雑誌名: Chem.Commun. ページ: 1906-1908
  • 査読あり
• [雑誌論文] Osmium Complexation of Mismatched DNA : Effect of the Bases Adjacent to Mismatched 5-Methylcytosine (2009)
  • 著者名/発表者名: Nomura A.; Tainaka K.; Okamoto A
  • 雑誌名: Bioconjugate Chem. 20(3) ページ: 603-607
  • 査読あり
• [雑誌論文] Doubly Thiazole Orange-Labeled DNA for Live Cell RNA Imaging (2009)
  • 著者名/発表者名: Kubota T.; Ikeda S.; Okamoto A
  • 雑誌名: Bull.Chem.Soc.Jpn. 82(1) ページ: 110-117
  • NAID: 10023987643
  • 査読あり
• [雑誌論文] Chemical approach toward efficient DNA methylation analysis (2009)
  • 著者名/発表者名: Okamoto A
  • 雑誌名: Org.Biomol.Chem. 7(1) ページ: 21-26
  • 査読あり
• [雑誌論文] Chemical approach toward efficient DNA methylation analysis (2009)
  • 著者名/発表者名: Okamoto, A.
  • 雑誌名: Org.Biomol.Chem. 7(1) ページ: 21-26
  • 査読あり
• [雑誌論文] Exciton-Controlled Hybridization-Sensitive Fluorescent Probes : Multicolor Detection of Nucleic Acids (2009)
  • 著者名/発表者名: Ikeda, S.
  • 雑誌名: Angew.Chem.,Int.Ed. 48(35) ページ: 6480-6484
  • 査読あり
• [雑誌論文] Heterogeneity of osmium oxidation efficiency at consecutive thymines (2008)
  • 著者名/発表者名: Nomura A.; Okamoto A
  • 雑誌名: Org.Biomol.Chem. 6(21) ページ: 3905-3907
  • 査読あり
• [雑誌論文] Sequence Dependence of Fluorescence Emission and Quenching of Doubly Thiazole Orange-Labeled DNA : Effective Design of a Hybridization-Sensitive Probe (2008)
  • 著者名/発表者名: Ikeda S.; Kubota T.; Kino K.; Okamoto A
  • 雑誌名: Bioconjugate Chem. 19(8) ページ: 1719-1725
  • 査読あり
• [雑誌論文] Hybridization-Sensitive On-Off DNA Probe : Application of the Exciton Coupling Effect to Effective Fluorescence Quenching (2008)
  • 著者名/発表者名: Ikeda S.; Okamoto A
  • 雑誌名: Chem.Asian J. 3(6) ページ: 958-968
  • 査読あり
• [雑誌論文] Synthesis and characterization of the 5-methyl-2'-deoxycytidine glycol-dioxoosmium-bipyridine ternary complex in DNA (2008)
  • 著者名/発表者名: Umemoto T.; Okamoto A
  • 雑誌名: Org.Biomol.Chem. 6(2) ページ: 269-271
  • 査読あり
• [雑誌論文] Design of a pyrene-containing fluorescence probe for labeling of RNA poly(A) tracts (2008)
  • 著者名/発表者名: Tanaka K.; Okamoto A
  • 雑誌名: Bioorg.Med.Chem. 16(1) ページ: 400-404
  • 査読あり
• [雑誌論文] Hybridization-Sensitive On-Off DNA Probe : Application of the Exciton Coupling Effect to Effective Fluorescence Quenching (2008)
  • 著者名/発表者名: Ikeda, S.
  • 雑誌名: Chem. Asian J. 3(6) ページ: 958-968
  • 査読あり
• [雑誌論文] Sequence Dependence of Fluorescence Emission and Quenching of Doubly Thiazole Orange-Labeled DNA : Effective Design of a Hybridization-Sensitive Probe (2008)
  • 著者名/発表者名: Ikeda, S.
  • 雑誌名: Bioconjugate Chem 19(8) ページ: 1719-1725
  • 査読あり
• [雑誌論文] Chemical approach toward efficient DNA methylation analysis (2008)
  • 著者名/発表者名: Okamoto, A.
  • 雑誌名: Org. Biomol. Chem. 7(1) ページ: 21-26
  • 査読あり
• [学会発表] Fluorescent Nucleotides for Efficient Nucleic Acid Sensing (2010)
  • 著者名/発表者名: Okamoto, A.
  • 学会等名: 11th International Chemistry Conference and Exhibition in Africa (11 ICCA)
  • 発表場所: Luxor, Egypt(Invited)
  • 年月日: 2010-11-20
• [学会発表] Photochemical design for live cell RNA imaging (2010)
  • 著者名/発表者名: Akimitsu Okamoto
  • 学会等名: International Chemical Congress of Pacific Basin Societies (PACIFICHEM 2010)
  • 発表場所: Honolulu, USA(Invited)
• [学会発表] DNA-osmium complex : Pinpoint detection of methylated cytosines (2010)
  • 著者名/発表者名: Akimitsu Okamoto
  • 学会等名: International Chemical Congress of Pacific Basin Societies (PACIFICHEM 2010)
  • 発表場所: Honolulu, USA(Invited)
• [学会発表] Artificial Zinc Finger Peptide for Recognition of Methylated DNA Duplex (2010)
  • 著者名/発表者名: Akiko Nomura, Akimitsu Okamoto
  • 学会等名: The 5th Asian Biological Inorganic Chemistry Conference
  • 発表場所: Kaohsiung, Taiwan
• [学会発表] Fluorescent Nucleotides for Efficient Nucleic Acid Sensin (2010)
  • 著者名/発表者名: Akimitsu Okamoto
  • 学会等名: 11th International Chemistry Conference and Exhibition in Africa (11 ICCA)
  • 発表場所: Luxor, Egypt(Invited)
• [学会発表] A short talk on ICON and ECHO probes (2010)
  • 著者名/発表者名: Akimitsu Okamoto
  • 学会等名: Asian 3 Roundtable on Nucleic Acids (A3RONA 2010 JAPAN)
  • 発表場所: Osaka, Japan(Invited)
• [学会発表] Application of artificial DNA probes in genome-scale DNA methylation analysis and evaluation of nucleic acid functions (2010)
  • 著者名/発表者名: Akimitsu Okamoto
  • 学会等名: BIG-CAS Seminar
  • 発表場所: Beijing, China(Invited)
• [学会発表] Hybridization-Sensitive Fluorescent Nucleic Acids for Live Cell RNA Imaging (2010)
  • 著者名/発表者名: Akimitsu Okamoto
  • 学会等名: 2nd Workshop on Fluorescence Chemosensor and Bio-imaging
  • 発表場所: Dalian, China(Invited)
• [学会発表] Exciton-controlled Hybridization-sensitive Fluorescent Oligonucleotides (2010)
  • 著者名/発表者名: Takeshi Kubota, Shuji Ikeda, Hiroyuki Yanagisawa, Mizue Yuki, Kaori Sugizaki, Akimitsu Okamoto
  • 学会等名: 29th International Round Table on Nucleosides, Nucleotides and Nucleic Acids (IRT 2010)
  • 発表場所: Lyon, France
• [学会発表] Thiazole Orange Dimer on a Nucleotide : Application to Live Cell RNA Imaging (2010)
  • 著者名/発表者名: Akimitsu Okamoto, Shuji Ikeda, Takeshi Kubota
  • 学会等名: 23th IUPAC Symposium on Photochemistry
  • 発表場所: Ferrara, Italy
• [学会発表] Hybridization-Sensitive Fluorescent Nucleic Acids for Effective RNA Imaging (2010)
  • 著者名/発表者名: Shuji Ikeda, Takeshi Kubota, Akimitsu Okamoto
  • 学会等名: Challenges in Organic Chemistry and Chemical Biology (ISACS1)
  • 発表場所: San Francisco, USA
• [学会発表] Addition of Osmium to DNA Pyrimidine Bases (2009)
  • 著者名/発表者名: 岡本晃充
  • 学会等名: 14th International Conference on Biological Inorganic Chemistry(ICBIC 14)
  • 発表場所: Nagoya Congress Center, Nagoya
  • 年月日: 2009-07-28
• [学会発表] ICON Probe : Osmium Complexation for Pinpoint Detection of Methylated Cytosines (2009)
  • 著者名/発表者名: Akimitsu Okamoto
  • 学会等名: BIT Life Sciences' 2nd Annual Congress and Expo of Molecular Diagnostics (CEMD-2009)
  • 発表場所: Beijing, China(Invited)
• [学会発表] Photochemical Probe Design for Multicolor RNA Imaging in Living Cells (2009)
  • 著者名/発表者名: Takeshi Kubota, Shuji Ikeda, Akimitsu Okamoto
  • 学会等名: 11th International Kyoto Conference on New Aspects of Organic Chemistry (IKCOC-11)
  • 発表場所: Kyoto, Japan
• [学会発表] Exciton-controlled fluorescence : Application to hybridization-sensitive fluorescent DNA probe (2009)
  • 著者名/発表者名: Akimitsu Okamoto, Shuji Ikeda, Takeshi Kubota, Mizue Yuki, Hiroyuki Yanagisawa
  • 学会等名: The 6th International symposium on Nucleic Acid Chemistry (61SNAC2009)
  • 発表場所: Takayama, Japan
• [学会発表] Doubly Dye-Labeled Nucleotide : Toward Efficient RNA Imaging (2009)
  • 著者名/発表者名: Akimitsu Okamoto
  • 学会等名: The 13th Asian Chemical Congress
  • 発表場所: Shanghai, China(Invited)
• [学会発表] Exciton-controlled probes for live cell RNA imaging (2009)
  • 著者名/発表者名: Akimitsu Okamoto
  • 学会等名: 42nd IUPAC Congress
  • 発表場所: Glasgow, UK(Invited)
• [学会発表] Addition of Osmium to DNA Pyrimidine Bases (2009)
  • 著者名/発表者名: Akimitsu Okamoto
  • 学会等名: 14th International Conference on Biological Inorganic Chemistry
  • 発表場所: Nagoya, Japan(Invited)
• [学会発表] Excitonic Interaction : New Photochemical Control for Nucleic Acid Imaging (2009)
  • 著者名/発表者名: Akimitsu Okamoto
  • 学会等名: The Fifth iCeMS International Symposium
  • 発表場所: Kyoto, Japan(Invited)
• [学会発表] Doubly Dye-Labeled Nucleotide: Synthesis, Photochemistry, and Application to RNA Imaging (2009)
  • 著者名/発表者名: Akimitsu Okamoto, Shuji Ikeda, Mizue Yuki, Takeshi Kubota
  • 学会等名: Tenth Tetrahedron Symposium
  • 発表場所: Paris, France
• [学会発表] DNA-Osmium Complex for DNA Methylation Analysis (2008)
  • 著者名/発表者名: 岡本晃充
  • 学会等名: 31st National Medicinal Chemistry Symposium
  • 発表場所: University of Pittsburgh
  • 年月日: 2008-06-18
• [学会発表] Control of DNA binding by a zinc finger peptide having and azobenzene photo-switch (2008)
  • 著者名/発表者名: Akiko Nomura, Akimitsu Okamoto
  • 学会等名: 4th Asian Biological Inorganic Chemistry Conference
  • 発表場所: Jeju, Korea
• [学会発表] Reactivity of Thymine Doublet in Single Strand DNA with Osmium Reagent (2008)
  • 著者名/発表者名: Akiko Nomura, Akimitsu Okamoto
  • 学会等名: joint Symposium of 18th International Roundtable on Nucleosides, Nucleotides and Nucleic Acids and 35th International Symposium on Nucleic Acids Chemistry
  • 発表場所: Kyoto, Japan
• [学会発表] Intracellular mRNA imaging with a hybridization sensitive fluorescent nucleotide (2008)
  • 著者名/発表者名: Takeshi Kubota, Shuji Ikeda, Akimitsu Okamoto
  • 学会等名: joint Symposium of 18th International Roundtable on Nucleosides, Nucleotides and Nucleic Acids and 35th International Symposium on Nucleic Acids Chemistry
  • 発表場所: Kyoto, Japan
• [学会発表] Design of a fluorescent probe for DNA/RNA imaging (2008)
  • 著者名/発表者名: Akimitsu Okamoto, Takeshi Kubota, Shuji Ikeda
  • 学会等名: joint Symposium of 18th International Roundtable on Nucleosides, Nucleotides and Nucleic Acids and 35th International Symposium on Nucleic Acids Chemistry
  • 発表場所: Kyoto, Japan
• [学会発表] Doubly Thiazole Orange-Labeled Nucleotide Application to Hybridization-Sensitive DNA Probe (2008)
  • 著者名/発表者名: Takeshi Kubota, Shuji Ikeda, Akimitsu Okamoto
  • 学会等名: 2008 Korea-Japan Symposium on Frontier Photoscience (KJFP 2008)
  • 発表場所: Jeju, Korea(Invited)
• [学会発表] DNA-Osmium Complex for DNA Methylation Analysis (2008)
  • 著者名/発表者名: Akimitsu Okamoto
  • 学会等名: 31st National Medicinal Chemistry Symposium, American Chemical Society
  • 発表場所: Pittsburgh, USA(Invited)
• [学会発表] Synthesis of bipyridine-adenine conjugates for DNA methylation analysis (2008)
  • 著者名/発表者名: Kazuki Tainaka, Akimitsu Okamoto
  • 学会等名: 14th Symposium on Chemistry of Nucleic Acid Components
  • 発表場所: Cesky Krumlov, Czech
• [図書] 実験医学増刊 生命現象の動的理解を目指すライブイメージング「第3章-5生細胞内mRNAの検出」 (2008)
  • 著者名/発表者名: 岡本晃充
  • 総ページ数: 7
  • 出版者: 羊土社
• [備考]
  • URL: http://www.riken.jp/lab-www/okamotoiru/indexj.htm