| 研究課題/領域番号 |
20F20406
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| 研究種目 |
特別研究員奨励費
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| 配分区分 | 補助金 |
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| 応募区分 | 外国 |
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| 審査区分 |
小区分48040:医化学関連
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| 研究機関 | 東京大学 |
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| 受入研究者 |
水島 昇 東京大学, 大学院医学系研究科(医学部), 教授 (10353434)
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| 外国人特別研究員 |
SOLTYSIK KAMIL 東京大学, 医学(系)研究科(研究院), 外国人特別研究員
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| 研究期間 (年度) |
2020-11-13 – 2023-03-31
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| 研究課題ステータス |
完了 (2022年度)
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| 配分額 *注記 |
2,400千円 (直接経費: 2,400千円)
2022年度: 400千円 (直接経費: 400千円)
2021年度: 1,200千円 (直接経費: 1,200千円)
2020年度: 800千円 (直接経費: 800千円)
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| キーワード | nuclear membrane / Organelle / Biomembrane / Proteome / Protein degradation / Nuclear structure |
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| 研究開始時の研究の概要 |
In starved cells, organelles are digested in bulk autophagy to supply nutrients for survival. But the concept of selective autophagy, of individually damaged organelles, gains increasing attention. One of the understudied substrates for selective autophagy is the inner nuclear membrane that faces chromatin and specializes in chromatin organization, cell cycle progression, and transcription. The machinery for autophagic degradation of nuclear membrane, in yeast, has recently been identified. However, in mammals, the substrates and meaning of this process are virtually not known.
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| 研究実績の概要 |
Over a period of two years, we have developed and optimized a proximity labeling system based on TurboID. This system was utilized to uncover novel nuclear membrane proteins via the covalent attachment of biotin to peripheral nuclear proteins. The biotinylated proteins were purified and subsequently identified using mass spectrometry. A series of biochemical and microscopic techniques further validated promising hits.
One of our top candidates, a previously uncharacterized E3-ubiquitin ligase was found to exist in a high molecular weight complex via blue native PAGE analysis. To identify the components of this complex, we pull down the E3 ligase under native conditions followed by mass spectrometry. An interactome revealed proteins involved in classic nuclear processes and suggested the E3’s role in metabolic regulation. Moreover, we confirmed that the E3 responds to the cell’s metabolic state.
Furthermore, we used ubiquitome analysis (PTM scan) to find candidate target proteins for the E3. Although numerous proteins were identified in this assay, further validation of the candidate list and replication of the result is warranted to draw a final conclusion.
This critical finding requires further investigation, and we hope that it will aid in our understanding of the role of nuclear membranes in the overall function of the cell.
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| 現在までの達成度 (段落) |
令和4年度が最終年度であるため、記入しない。
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| 今後の研究の推進方策 |
令和4年度が最終年度であるため、記入しない。
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