| 研究課題/領域番号 |
20J11059
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| 研究種目 |
特別研究員奨励費
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| 配分区分 | 補助金 |
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| 応募区分 | 国内 |
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| 審査区分 |
小区分62010:生命、健康および医療情報学関連
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| 研究機関 | 東京工業大学 |
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研究代表者 |
MASUKAWA Marcos 東京工業大学, 情報理工学院, 特別研究員(DC2)
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| 研究期間 (年度) |
2020-04-24 – 2022-03-31
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| 研究課題ステータス |
完了 (2021年度)
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| 配分額 *注記 |
1,700千円 (直接経費: 1,700千円)
2021年度: 800千円 (直接経費: 800千円)
2020年度: 900千円 (直接経費: 900千円)
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| キーワード | DNA origami / colloidosome / self-assembly / aqueous two-phase system / aqueous-aqueous emulsion / DNA hydrogel / purification / monodisperse / microfluidic |
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| 研究開始時の研究の概要 |
DNA and proteins can be inserted into the cells to modify their metabolism and genetic material. This process is called transfection and it is a key technique needed in gene editing, a prospective cure for degenerative diseases. To increase the functionality of transfected structures, we are developing a DNA hydrogel whose biological function can be activated by a chemical signal. Inside the cells, these structures can perform as molecular computers, processing signals and producing outputs within the cell. These cell hybrids will lead to novel diagnostics and treatments.
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| 研究実績の概要 |
In recent research, the interaction between self-assembled DNA structures and liquid-liquid phase-separated systems composed of polymers was investigated. It was observed that DNA structures can be selectively taken up by these compartments, leading to self-assembly that mimics artificial cells and the interaction of nucleic acids and organelles. Based on these findings, I developed a method for purifying self-assembled DNA structures and created a new category of colloidossomes based on the accumulation of DNA origami at the interface of droplets. This enabled the creation of colloidosomes that can self-organise into cellular like structures. In the future, I will explore the possibility of translocating nucleic through membranes of DNA coloidosomes, which might find application in DNA sequencing technology and on synthetic cell models for the transport of nucleic acids thourgh membranes.
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| 現在までの達成度 (段落) |
令和3年度が最終年度であるため、記入しない。
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| 今後の研究の推進方策 |
令和3年度が最終年度であるため、記入しない。
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