研究課題/領域番号 |
20J15151
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研究種目 |
特別研究員奨励費
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配分区分 | 補助金 |
応募区分 | 国内 |
審査区分 |
小区分34020:分析化学関連
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研究機関 | 京都大学 |
研究代表者 |
劉 晨晨 京都大学, 工学研究科, 特別研究員(PD)
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研究期間 (年度) |
2020-04-24 – 2022-03-31
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研究課題ステータス |
完了 (2021年度)
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配分額 *注記 |
1,900千円 (直接経費: 1,900千円)
2021年度: 900千円 (直接経費: 900千円)
2020年度: 1,000千円 (直接経費: 1,000千円)
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キーワード | poly(ethylene glycol) / protein analysis / affinity electrophoresis / electrophoresis / DNA analysis |
研究開始時の研究の概要 |
A poly(ethylene glycol) (PEG) gel-based molecularly imprinted polymer (MIP) is studied aiming at enhanced separation performance for proteins. The gel-based MIP is promising in separation science but suffers from low reproducibility. For solving this issue, a novel PEG gel with enhanced mechanical property will be investigated.
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研究実績の概要 |
The poly(ethylene glycol) (PEG) based molecularly imprinted hydrogel (MIH) was fabricated for the molecular recognition of the target protein. Copoly(PEG acrylate/PEG diacrylate) (copoly(PEGA/PEGDA)) hydrogel was employed in the preparation of MIH for cytochrome c recognition. A highly specific molecular recognition was realized when using the MIH as a sorbent, evidenced by (1) the MIH showed adsorption to the cytochrome c target while the non-imprinted hydrogel showed no adsorption to the target, and (2) the MIH hydrogel showed higher adsorption to the cytochrome c target than to the other unspecific proteins. When tested as the affinity substance in electrophoresis, the MIH retarded the migration of cytochrome c to a higher extent compared with the other unspecific proteins.
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現在までの達成度 (段落) |
令和3年度が最終年度であるため、記入しない。
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今後の研究の推進方策 |
令和3年度が最終年度であるため、記入しない。
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