| 研究開始時の研究の概要 |
With circulating free DNA (cfDNA) hybridization-based analysis, most genetic diseases can be identified. However, challenging factors are: (1) the low concentration and small size of cfDNA, and (2) the reannealing between a complementary single-stranded DNA (ssDNA) resulting in lower efficiency analysis. Thus, a device that can retain ssDNA in room temperature will enhance the sensitivity. Here we purpose microheater nanowire microfluidic devices for analyzing methylated cfDNA via the combination of capturing ssDNA on nanowire surfaces, followed-by sequence-specific detection of target DNA.
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| 研究実績の概要 |
The development of nanowire devices integrated with a microheater (Microheater - ZnO nanowire device) as nucleic acid detection platform using in situ annealed Serinol Nucleic Acid (SNA) probes was demonstrated. First, the microheater could constantly generate suitable heat was confirmed. Second, the use of SNA-MBs to detect target ssDNAs specifically was confirmed. By combining the unique properties of ZnO-NWs and the microheater, the integrated device could detect target ssDNAs using in-situ annealed probes was confirmed. This confirms that this microheater-ZnO-NW device will be applied to fundamental studies related to nanobiodevice-based DNA detection.
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