| 研究開始時の研究の概要 |
In last decades, there are increasing number of diseases caused by rapid-mutated Influenza virus and similar transmitted disease, such as mosquitoes-transmitted disease of flavivirus family. This research aims to develop a biosensor with nanocarrier system for indicator molecules. This biosensor can exhibit unique signal for each target virus to establish the identification system with high sensitivity to detect low virus concentration. With this concept of biosensor, the biosensor is possibly established in colorimetric and electrochemical-based detection for on-site paper-based platform.
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| 研究実績の概要 |
In the last year of this fellowship, the aim focused on developing highly sensitive biosensor for virus detection. Compared to previous year, the developed chromogenic nanocarrier using encapsulation is not suitable for electrochemical sensing system. Then, we adjusted the method and chelating the signal molecules carried within the aptamer network on the electrode to amplify the signal in the electrochemical detection (Analytica Chimica Acta, 1207, 339817 (2022))
On the other point, understanding the limitation of the signal amplification and uniformity of the nanocarrier, this year, metal-organic framework, a recent uprising nanocarrier, was prepared together with small nanozyme Pt to amplify detection signal for colorimetric biosensor. Here, the nanozyme (NZ) showcased highly sensitive detection limit which is superior to the precedent research of this nanozyme-based immunoassay. (Biosensors and Bioelectronics, 126, 114602, (2022)).
Several works have also been done however those works are not yet done in reporting to the society, on how we improve the immunoassay-based biosensor for higher reliability in the detection and how to implement the nanozyme-based detection to the paper-based immunoassay. Those pending writings are the drawback of this year progress.
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