| 研究課題/領域番号 |
20K16262
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| 研究種目 |
若手研究
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| 配分区分 | 基金 |
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| 審査区分 |
小区分49060:ウイルス学関連
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| 研究機関 | 金沢大学 |
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研究代表者 |
LIM KEE・SIANG 金沢大学, ナノ生命科学研究所, 特任助教 (60842987)
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| 研究期間 (年度) |
2020-04-01 – 2024-03-31
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| 研究課題ステータス |
完了 (2023年度)
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| 配分額 *注記 |
4,160千円 (直接経費: 3,200千円、間接経費: 960千円)
2023年度: 780千円 (直接経費: 600千円、間接経費: 180千円)
2022年度: 910千円 (直接経費: 700千円、間接経費: 210千円)
2021年度: 1,170千円 (直接経費: 900千円、間接経費: 270千円)
2020年度: 1,300千円 (直接経費: 1,000千円、間接経費: 300千円)
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| キーワード | HS-AFM nanoimaging / viral fusion protein / Hemagglutinin / Spike protein / Influenza A / SARS-CoV-2 / SARS-CoV-2 spike protein / High-speed AFM / Neutralizing antibodies / COVID-19 / HS-AFM / Viral Fusion Protein / Viral fusion protein / fusogenic transition / exosome / Direct visualization / Virulence factors |
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| 研究開始時の研究の概要 |
HS-AFM is an ideal tool for investigating properties of viral fusion proteins that include native conformation, binding activity with host factors, and structural dynamic during fusogenic transition. Furthermore, interaction between fusion proteins and exosomes will be also be recorded.
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| 研究実績の概要 |
From 2020-2023, the applicant conducted HS-AFM nanoimaging to study viral fusion proteins like hemagglutinin (HA; Influenza A) and spike protein (S protein; SARS-CoV-2). The results indicate HS-AFM's capability in capturing nanoscale structural dynamics of these proteins, including fusogenic transitions of HA, S protein-ACE2 interactions, and more. These transient structures are challenging to observe with cryo-EM and X-ray crystallography. Additionally, the applicant investigated neutralizing antibody-S protein interactions. Overall, the applicant showcases HS-AFM's potential for studying viral fusion proteins and identifying potential antiviral targets.
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